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The map label for this gene is nuoM [H]

Identifier: 157371534

GI number: 157371534

Start: 3649875

End: 3651404

Strand: Reverse

Name: nuoM [H]

Synonym: Spro_3297

Alternate gene names: 157371534

Gene position: 3651404-3649875 (Counterclockwise)

Preceding gene: 157371535

Following gene: 157371533

Centisome position: 67.01

GC content: 55.42

Gene sequence:

>1530_bases
ATGCTATTACCTTGGCTAATTCTTATCCCCTTTATTGGCGGTCTGCTGTGCTGGCAGTTTGAGCGCTTCGGTACTAAGGT
GCCGCGCTGGATTGCGTTGATCGCAATGGGGCTGACATTGGCGCTTTCTCTGTATCTGTGGATGCAGGGTGGCTACACAT
TGACTACGCCGAAAGGCATTCCGCAGTGGCAGTCTGAATTCCTGCTGCCGTGGATCCCACGCTTTGGTATTTCCATCCAC
CTGGCGCTGGACGGCCTGTCACTGCTGATGGTGGTGCTGACCGGCTTGCTGGGCGTGCTGGCCATTCTCTGTTCCTGGCG
TGAAATCCAGAAATACCAGGGCTTCTTCCACCTGAACCTGCTGTGGATCCTGGGTGGGGTAATCGGCGTGTTCCTCGCCA
TCGACATGTTCCTGTTCTTCTTCTTCTGGGAAATGATGTTGGTGCCGATGTACTTCCTGATTGCACTCTGGGGTCACAAG
GCGTCGGACGGGAAGACCCGTATCACTGCGGCTACCAAGTTCTTCATCTATACCCAGGCCAGTGGTCTGGTGATGTTGAT
TGCCATTATGGGCCTGGTGTTCGTGCACTATAACGCGACCGGCGTGTGGACCTTCGATTACGAAGACCTGCTGCAGACGC
CAATGTCCCATAACGTGCAATACCTGTTGATGCTGGGCTTCTTCATCGCCTTTGCGGTGAAAATGCCGGTGGTGCCATTG
CATGGCTGGTTGCCTGATGCGCATAGCCAGGCACCCACCGCAGGTTCTGTTGACTTGGCGGGGATCTTGCTGAAAACCGC
GGCCTACGGCATGTTGCGTTTCAGCCTGCCGCTGTTCCCGGAAGCGTCGCACGAGTTTGCGCCAATCGCCATGTGGCTGG
GCGTGGTCGGTATCTTCTACGGTGCCTGGATGGCATTTGCGCAGACTGATATCAAGCGTCTGATTGCTTATACCTCGGTT
TCCCATATGGGCTTCGTGCTGATTGCCATCTACACCGGCAGCCAACTGGCTTACCAGGGTGCGGTGATCCAGATGATTGC
GCACGGTCTGTCTGCGGCCGGTATGTTCATCATCTGTGGCCAGCTGTACGAACGTCTGCATACCCGCGACATGCGTGAAA
TGGGCGGCCTGTGGGGACGTATTAAATACATCCCGGCGCTGTCGCTGTTCTTCGCAGTGGCGACCCTGGGGATGCCGGGT
ACCGGTAACTTCGTCGGTGAATTCATGATCCTGTTCGGCAGCTATCAGGTTGTGCCGGTGATCACCGTGATTTCTACCTT
CGGTCTGGTGTTTGCTTCGGTTTACTCGTTGATCATGATGCAGCGCGCCTATTACGGCAAAGCCAAGTCTGACCAACCGT
TACCGGGCATGACCGCGCGTGAGCTGTTCATCATTCTGCTGCTGGTGGTGTTGCTGGTTCTGCTGGGGGTTTACCCGCAG
CCGATTCTGGATACTTCCAATGCGGCGATGAGCAACGTGCAACACTGGTTTGGTTCGTCAGTTTCAGCAATTTCAACAAC
AAGGCCGTAA

Upstream 100 bases:

>100_bases
TTGAATAATTTAAACAGACGCATTTTTCGGGCGTAGCGCCCTGCAGGGCAGGCGCTACGTACAAAGTTTGAAATTATTCG
TATAAGGGACACAAAACGCC

Downstream 100 bases:

>100_bases
TTCGCCATGACAATAACTCCTCAACAACTGATCGCATTGCTACCACTGTTGATCGTCGGATTGACGGTGGTGGTTGTGAT
GCTAGGCATTGCGTGGCGAC

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M; NUO13 [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MLLPWLILIPFIGGLLCWQFERFGTKVPRWIALIAMGLTLALSLYLWMQGGYTLTTPKGIPQWQSEFLLPWIPRFGISIH
LALDGLSLLMVVLTGLLGVLAILCSWREIQKYQGFFHLNLLWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
ASDGKTRITAATKFFIYTQASGLVMLIAIMGLVFVHYNATGVWTFDYEDLLQTPMSHNVQYLLMLGFFIAFAVKMPVVPL
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGMLRFSLPLFPEASHEFAPIAMWLGVVGIFYGAWMAFAQTDIKRLIAYTSV
SHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGMFIICGQLYERLHTRDMREMGGLWGRIKYIPALSLFFAVATLGMPG
TGNFVGEFMILFGSYQVVPVITVISTFGLVFASVYSLIMMQRAYYGKAKSDQPLPGMTARELFIILLLVVLLVLLGVYPQ
PILDTSNAAMSNVQHWFGSSVSAISTTRP

Sequences:

>Translated_509_residues
MLLPWLILIPFIGGLLCWQFERFGTKVPRWIALIAMGLTLALSLYLWMQGGYTLTTPKGIPQWQSEFLLPWIPRFGISIH
LALDGLSLLMVVLTGLLGVLAILCSWREIQKYQGFFHLNLLWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
ASDGKTRITAATKFFIYTQASGLVMLIAIMGLVFVHYNATGVWTFDYEDLLQTPMSHNVQYLLMLGFFIAFAVKMPVVPL
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGMLRFSLPLFPEASHEFAPIAMWLGVVGIFYGAWMAFAQTDIKRLIAYTSV
SHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGMFIICGQLYERLHTRDMREMGGLWGRIKYIPALSLFFAVATLGMPG
TGNFVGEFMILFGSYQVVPVITVISTFGLVFASVYSLIMMQRAYYGKAKSDQPLPGMTARELFIILLLVVLLVLLGVYPQ
PILDTSNAAMSNVQHWFGSSVSAISTTRP
>Mature_509_residues
MLLPWLILIPFIGGLLCWQFERFGTKVPRWIALIAMGLTLALSLYLWMQGGYTLTTPKGIPQWQSEFLLPWIPRFGISIH
LALDGLSLLMVVLTGLLGVLAILCSWREIQKYQGFFHLNLLWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
ASDGKTRITAATKFFIYTQASGLVMLIAIMGLVFVHYNATGVWTFDYEDLLQTPMSHNVQYLLMLGFFIAFAVKMPVVPL
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGMLRFSLPLFPEASHEFAPIAMWLGVVGIFYGAWMAFAQTDIKRLIAYTSV
SHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGMFIICGQLYERLHTRDMREMGGLWGRIKYIPALSLFFAVATLGMPG
TGNFVGEFMILFGSYQVVPVITVISTFGLVFASVYSLIMMQRAYYGKAKSDQPLPGMTARELFIILLLVVLLVLLGVYPQ
PILDTSNAAMSNVQHWFGSSVSAISTTRP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=360, Percent_Identity=33.0555555555556, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI1788613, Length=512, Percent_Identity=87.3046875, Blast_Score=884, Evalue=0.0,
Organism=Escherichia coli, GI1788827, Length=441, Percent_Identity=24.7165532879819, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788831, Length=312, Percent_Identity=28.8461538461538, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1788614, Length=516, Percent_Identity=26.1627906976744, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI145693160, Length=433, Percent_Identity=25.4041570438799, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1788829, Length=234, Percent_Identity=27.3504273504274, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 56654; Mature: 56654

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLPWLILIPFIGGLLCWQFERFGTKVPRWIALIAMGLTLALSLYLWMQGGYTLTTPKGI
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
PQWQSEFLLPWIPRFGISIHLALDGLSLLMVVLTGLLGVLAILCSWREIQKYQGFFHLNL
CCHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHKASDGKTRITAATKFFIYTQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECC
SGLVMLIAIMGLVFVHYNATGVWTFDYEDLLQTPMSHNVQYLLMLGFFIAFAVKMPVVPL
CHHHHHHHHHHHHHHEECCCEEEEECHHHHHCCCHHCCHHHHHHHHHHHHHHHHCCCEEC
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGMLRFSLPLFPEASHEFAPIAMWLGVVGIFY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
GAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGMFIICG
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
QLYERLHTRDMREMGGLWGRIKYIPALSLFFAVATLGMPGTGNFVGEFMILFGSYQVVPV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHH
ITVISTFGLVFASVYSLIMMQRAYYGKAKSDQPLPGMTARELFIILLLVVLLVLLGVYPQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
PILDTSNAAMSNVQHWFGSSVSAISTTRP
CCCCCCHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLLPWLILIPFIGGLLCWQFERFGTKVPRWIALIAMGLTLALSLYLWMQGGYTLTTPKGI
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
PQWQSEFLLPWIPRFGISIHLALDGLSLLMVVLTGLLGVLAILCSWREIQKYQGFFHLNL
CCHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHKASDGKTRITAATKFFIYTQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECC
SGLVMLIAIMGLVFVHYNATGVWTFDYEDLLQTPMSHNVQYLLMLGFFIAFAVKMPVVPL
CHHHHHHHHHHHHHHEECCCEEEEECHHHHHCCCHHCCHHHHHHHHHHHHHHHHCCCEEC
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGMLRFSLPLFPEASHEFAPIAMWLGVVGIFY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
GAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGMFIICG
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
QLYERLHTRDMREMGGLWGRIKYIPALSLFFAVATLGMPGTGNFVGEFMILFGSYQVVPV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHH
ITVISTFGLVFASVYSLIMMQRAYYGKAKSDQPLPGMTARELFIILLLVVLLVLLGVYPQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
PILDTSNAAMSNVQHWFGSSVSAISTTRP
CCCCCCHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]