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The map label for this gene is nuoN [H]

Identifier: 157371533

GI number: 157371533

Start: 3648411

End: 3649868

Strand: Reverse

Name: nuoN [H]

Synonym: Spro_3296

Alternate gene names: 157371533

Gene position: 3649868-3648411 (Counterclockwise)

Preceding gene: 157371534

Following gene: 157371531

Centisome position: 66.98

GC content: 58.37

Gene sequence:

>1458_bases
ATGACAATAACTCCTCAACAACTGATCGCATTGCTACCACTGTTGATCGTCGGATTGACGGTGGTGGTTGTGATGCTAGG
CATTGCGTGGCGACGCGACCACTTTATCAACGCCACCCTGACCGTGATCGGTCTAAACCTGGCGCTGCTTTCGCTGTACT
TTGTCGGCCAGGCAGGCCCAATGGACGTCACCCCGCTGCTGCGGGTTGACGGCTACTCGATGTTCTATACCGGGCTGGTG
ATTCTGGCGAGTCTGGCGACCTGTACCTTTGCCTATCCTTGGCTGGTCGGTTACCCGGACAACCGCGAAGAGTTCTACCT
GCTGGTGCTGATTGCTACCCTGGGTGGCATTCTGCTGGCGAGTGCCAACCATCTGGCGTCGCTGTTCCTCGGTATTGAAC
TGATCTCGCTGCCGCTGTTCGGTCTGGTGGGTTATGCCTACCGCCAGAAGCGTCCGCTGGAAGCGGCCATCAAGTACATG
TTGCTGTCCGCTGCCGCGTCGTCATTCCTGCTGTTTGGTATGGCGCTGCTGTATGCCGAGTCCGGCGACCTGTCACTGGC
TGGCCTGGGCAAAAGCCTGCAAGAAAACATGATGCACCAGCCGCTGGTCCTGGCGGGTATGGGCATGATGATTGTCGGTC
TGGGCTTCAAGCTGTCGCTGGTGCCGTTCCAACTGTGGACGCCGGATGTGTATCAGGGTGCGCCTGCGCCGGTTTCAACC
TTCCTGGCGACCGCCAGCAAGATTGCAATCTTCGCCGTAGTGATGCGTTTGTTCCTGTATGCACCGGCCGCCGACAACGA
AGCGCTGCGCATGGTGCTGTCAATCATTGCCGTCTGCTCAATTTTGTTCGGCAACCTGATGGCTATCAGCCAGACCAATA
TCAAGCGTCTGCTGGGTTACTCGTCGATCGCCCACCTGGGTTATCTGCTGGTGGCGTTGATTGCGGTGCAAACCCATCAG
CTGTCGTTGGAAACCGCCGGGGTTTACCTGGCCGGTTACCTGTTCAGCAGCCTGGGCGCGTTTGGCGTCGTCAGCCTGAT
GTCCAGCCCGTACCGTGGCCCGGATGCCGATTCACTGTTCTCCTACCGTGGTCTGTTCTGGCATAAGCCAATTCTGTCTG
CGGTAATGACGGTGATGATGCTGTCACTGGCCGGTATCCCGATGACTCTGGGCTTTATCGGTAAGTTCTTCGTTATCGCG
ATGGGCGTCAGTGCTCACCTGTGGTGGTTGACCGGCGCAGTGGTGCTGGGTAGTGCCATCGGTTTGTACTACTACCTGCG
TGTAACCGTCAGCCTGTTCCTCAGCGCGCCGGAAGCGCTGCAGCGCGATACCCCGAACAACTGGGCGTTGACCGCAGGCG
GTGTGGTGGTGTTGATCTCCGCCGCGTTGGTGTTGCTGCTGGGGGTTTATCCGCAGCCGCTGATTACGCTGGTGCAGATG
GCACAGCCGATGTTCTGA

Upstream 100 bases:

>100_bases
GCAGCCGATTCTGGATACTTCCAATGCGGCGATGAGCAACGTGCAACACTGGTTTGGTTCGTCAGTTTCAGCAATTTCAA
CAACAAGGCCGTAATTCGCC

Downstream 100 bases:

>100_bases
TTGGCATCAAGGCAGTAAAAAAGGTCGCTTCGGCGACCTTTTTTTATGGGGACGGGGAAGTGATGCAGTACTGGAACGAT
CAACCGGGATTGTTTTTAAA

Product: NADH dehydrogenase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MTITPQQLIALLPLLIVGLTVVVVMLGIAWRRDHFINATLTVIGLNLALLSLYFVGQAGPMDVTPLLRVDGYSMFYTGLV
ILASLATCTFAYPWLVGYPDNREEFYLLVLIATLGGILLASANHLASLFLGIELISLPLFGLVGYAYRQKRPLEAAIKYM
LLSAAASSFLLFGMALLYAESGDLSLAGLGKSLQENMMHQPLVLAGMGMMIVGLGFKLSLVPFQLWTPDVYQGAPAPVST
FLATASKIAIFAVVMRLFLYAPAADNEALRMVLSIIAVCSILFGNLMAISQTNIKRLLGYSSIAHLGYLLVALIAVQTHQ
LSLETAGVYLAGYLFSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHKPILSAVMTVMMLSLAGIPMTLGFIGKFFVIA
MGVSAHLWWLTGAVVLGSAIGLYYYLRVTVSLFLSAPEALQRDTPNNWALTAGGVVVLISAALVLLLGVYPQPLITLVQM
AQPMF

Sequences:

>Translated_485_residues
MTITPQQLIALLPLLIVGLTVVVVMLGIAWRRDHFINATLTVIGLNLALLSLYFVGQAGPMDVTPLLRVDGYSMFYTGLV
ILASLATCTFAYPWLVGYPDNREEFYLLVLIATLGGILLASANHLASLFLGIELISLPLFGLVGYAYRQKRPLEAAIKYM
LLSAAASSFLLFGMALLYAESGDLSLAGLGKSLQENMMHQPLVLAGMGMMIVGLGFKLSLVPFQLWTPDVYQGAPAPVST
FLATASKIAIFAVVMRLFLYAPAADNEALRMVLSIIAVCSILFGNLMAISQTNIKRLLGYSSIAHLGYLLVALIAVQTHQ
LSLETAGVYLAGYLFSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHKPILSAVMTVMMLSLAGIPMTLGFIGKFFVIA
MGVSAHLWWLTGAVVLGSAIGLYYYLRVTVSLFLSAPEALQRDTPNNWALTAGGVVVLISAALVLLLGVYPQPLITLVQM
AQPMF
>Mature_484_residues
TITPQQLIALLPLLIVGLTVVVVMLGIAWRRDHFINATLTVIGLNLALLSLYFVGQAGPMDVTPLLRVDGYSMFYTGLVI
LASLATCTFAYPWLVGYPDNREEFYLLVLIATLGGILLASANHLASLFLGIELISLPLFGLVGYAYRQKRPLEAAIKYML
LSAAASSFLLFGMALLYAESGDLSLAGLGKSLQENMMHQPLVLAGMGMMIVGLGFKLSLVPFQLWTPDVYQGAPAPVSTF
LATASKIAIFAVVMRLFLYAPAADNEALRMVLSIIAVCSILFGNLMAISQTNIKRLLGYSSIAHLGYLLVALIAVQTHQL
SLETAGVYLAGYLFSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHKPILSAVMTVMMLSLAGIPMTLGFIGKFFVIAM
GVSAHLWWLTGAVVLGSAIGLYYYLRVTVSLFLSAPEALQRDTPNNWALTAGGVVVLISAALVLLLGVYPQPLITLVQMA
QPMF

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=336, Percent_Identity=24.702380952381, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI145693160, Length=484, Percent_Identity=81.6115702479339, Blast_Score=793, Evalue=0.0,
Organism=Escherichia coli, GI1788829, Length=324, Percent_Identity=27.1604938271605, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788831, Length=204, Percent_Identity=29.4117647058824, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788614, Length=202, Percent_Identity=28.7128712871287, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1788613, Length=317, Percent_Identity=25.5520504731861, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 52243; Mature: 52112

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITPQQLIALLPLLIVGLTVVVVMLGIAWRRDHFINATLTVIGLNLALLSLYFVGQAGP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
MDVTPLLRVDGYSMFYTGLVILASLATCTFAYPWLVGYPDNREEFYLLVLIATLGGILLA
CCCCCHHEECCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHH
SANHLASLFLGIELISLPLFGLVGYAYRQKRPLEAAIKYMLLSAAASSFLLFGMALLYAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGDLSLAGLGKSLQENMMHQPLVLAGMGMMIVGLGFKLSLVPFQLWTPDVYQGAPAPVST
CCCEEHHHCCHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCHHHCCCCCHHHH
FLATASKIAIFAVVMRLFLYAPAADNEALRMVLSIIAVCSILFGNLMAISQTNIKRLLGY
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSIAHLGYLLVALIAVQTHQLSLETAGVYLAGYLFSSLGAFGVVSLMSSPYRGPDADSLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
SYRGLFWHKPILSAVMTVMMLSLAGIPMTLGFIGKFFVIAMGVSAHLWWLTGAVVLGSAI
HHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLYYYLRVTVSLFLSAPEALQRDTPNNWALTAGGVVVLISAALVLLLGVYPQPLITLVQM
HHHHHHHHHHHHHHCCCHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHH
AQPMF
HCCCC
>Mature Secondary Structure 
TITPQQLIALLPLLIVGLTVVVVMLGIAWRRDHFINATLTVIGLNLALLSLYFVGQAGP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
MDVTPLLRVDGYSMFYTGLVILASLATCTFAYPWLVGYPDNREEFYLLVLIATLGGILLA
CCCCCHHEECCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHH
SANHLASLFLGIELISLPLFGLVGYAYRQKRPLEAAIKYMLLSAAASSFLLFGMALLYAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGDLSLAGLGKSLQENMMHQPLVLAGMGMMIVGLGFKLSLVPFQLWTPDVYQGAPAPVST
CCCEEHHHCCHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCHHHCCCCCHHHH
FLATASKIAIFAVVMRLFLYAPAADNEALRMVLSIIAVCSILFGNLMAISQTNIKRLLGY
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSIAHLGYLLVALIAVQTHQLSLETAGVYLAGYLFSSLGAFGVVSLMSSPYRGPDADSLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
SYRGLFWHKPILSAVMTVMMLSLAGIPMTLGFIGKFFVIAMGVSAHLWWLTGAVVLGSAI
HHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLYYYLRVTVSLFLSAPEALQRDTPNNWALTAGGVVVLISAALVLLLGVYPQPLITLVQM
HHHHHHHHHHHHHHCCCHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHH
AQPMF
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA