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The map label for this gene is yeiE [H]

Identifier: 157371462

GI number: 157371462

Start: 3555185

End: 3556060

Strand: Reverse

Name: yeiE [H]

Synonym: Spro_3225

Alternate gene names: 157371462

Gene position: 3556060-3555185 (Counterclockwise)

Preceding gene: 157371465

Following gene: 157371461

Centisome position: 65.26

GC content: 57.19

Gene sequence:

>876_bases
ATGCATATCACCTTACGTCAATTGGAAGTGTTTACCGAAGTTCTTAAGAGCGGTTCGACCACGCAGGCTTCGGTAGTTCT
GGCGTTATCACAATCGGCGGTCAGCGCCGCGCTGGCCGATCTGGAAGGGCAATTGGGCGTGCAGTTGTTTGACCGCGTGG
GCAAGCGTTTGGTCATTAATGAACATGGCAGGTTGCTGTACCCGAAGGCGCTGGCACTGCTGGAGCAGGCGGGGGAGATT
GAACAACTGTTCCGCCATGACGGCGGTGCATTGCGCATCAGTGCCAGCAGCACCATCGGTAACTATATGCTGCCGGAGAT
GATCGCCCATTATCGGCATGATTTCCCCGCTTCACCGTTGGAACTGAACGTCGGCAACAGCCAGGATGTGATCGGCGCTG
TGGCGGATTTCCGTGTCGATCTGGGACTGATTGAAGGCCCTTGTCATATGCCGGAACTGATCACTCTGCCTTGGCTGGAA
GATGAATTGGTGGTATTTGCCGCGCCGGACAACCCGTTGGCCGGGCAACCACTGACCCTGGAAATGCTGGCCACAGCCCC
CTGGATCCTGCGTGAGGGTGGATCGGGGACGCGCGAGGTGCTGCACCATCTGCTGCTGGCGCACTTGCCGCACTTCCAAC
TGGTGATGGAACTGGGTAATTCGGAAGCGATCAAACATGCGGTTCGCCATGGCATGGGGATCAGTTGCCTGTCGCGGCGT
GTGATTGGCGAACAGTTGGCCAGTGGCGTGCTGGTGGAGCTGAATATCCCGTTACCGCCGCTGATGCGCACCCTTTATCT
GATCCATCACCGGCAAAAGCATATCTCCAACGCGCTGCAGCGTTTTCTCGGATACTGTGGGTTTCAGCCTCAATAG

Upstream 100 bases:

>100_bases
ATAGTATTCACCCTGTGCGTTGTTGCCGGTCAGCAGCGCAGATTTTGCATTAATTTCAGTGACTTTTTTCGATGTCACCT
TTGCTTAAAACCGAGACGCT

Downstream 100 bases:

>100_bases
CGTCTCCGAAAGCAGTAAAAAAGCATAAAAAGCTGCTCCCTGTCACATTAATGACAGTAAGTAAGGTATTCTGGCCTGTC
GCTAATAATTAGCCCTGAAT

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MHITLRQLEVFTEVLKSGSTTQASVVLALSQSAVSAALADLEGQLGVQLFDRVGKRLVINEHGRLLYPKALALLEQAGEI
EQLFRHDGGALRISASSTIGNYMLPEMIAHYRHDFPASPLELNVGNSQDVIGAVADFRVDLGLIEGPCHMPELITLPWLE
DELVVFAAPDNPLAGQPLTLEMLATAPWILREGGSGTREVLHHLLLAHLPHFQLVMELGNSEAIKHAVRHGMGISCLSRR
VIGEQLASGVLVELNIPLPPLMRTLYLIHHRQKHISNALQRFLGYCGFQPQ

Sequences:

>Translated_291_residues
MHITLRQLEVFTEVLKSGSTTQASVVLALSQSAVSAALADLEGQLGVQLFDRVGKRLVINEHGRLLYPKALALLEQAGEI
EQLFRHDGGALRISASSTIGNYMLPEMIAHYRHDFPASPLELNVGNSQDVIGAVADFRVDLGLIEGPCHMPELITLPWLE
DELVVFAAPDNPLAGQPLTLEMLATAPWILREGGSGTREVLHHLLLAHLPHFQLVMELGNSEAIKHAVRHGMGISCLSRR
VIGEQLASGVLVELNIPLPPLMRTLYLIHHRQKHISNALQRFLGYCGFQPQ
>Mature_291_residues
MHITLRQLEVFTEVLKSGSTTQASVVLALSQSAVSAALADLEGQLGVQLFDRVGKRLVINEHGRLLYPKALALLEQAGEI
EQLFRHDGGALRISASSTIGNYMLPEMIAHYRHDFPASPLELNVGNSQDVIGAVADFRVDLGLIEGPCHMPELITLPWLE
DELVVFAAPDNPLAGQPLTLEMLATAPWILREGGSGTREVLHHLLLAHLPHFQLVMELGNSEAIKHAVRHGMGISCLSRR
VIGEQLASGVLVELNIPLPPLMRTLYLIHHRQKHISNALQRFLGYCGFQPQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=286, Percent_Identity=78.3216783216783, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI1788296, Length=293, Percent_Identity=29.6928327645051, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI157672245, Length=284, Percent_Identity=27.8169014084507, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788748, Length=286, Percent_Identity=26.2237762237762, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI87082132, Length=288, Percent_Identity=25.6944444444444, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1786401, Length=304, Percent_Identity=25.6578947368421, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787530, Length=244, Percent_Identity=26.2295081967213, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789204, Length=297, Percent_Identity=28.2828282828283, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789506, Length=195, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787879, Length=247, Percent_Identity=26.3157894736842, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787601, Length=197, Percent_Identity=24.3654822335025, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI145693105, Length=294, Percent_Identity=23.8095238095238, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789639, Length=146, Percent_Identity=28.7671232876712, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788297, Length=240, Percent_Identity=23.3333333333333, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31957; Mature: 31957

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHITLRQLEVFTEVLKSGSTTQASVVLALSQSAVSAALADLEGQLGVQLFDRVGKRLVIN
CCCHHHHHHHHHHHHHCCCCCHHHEEEEHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEE
EHGRLLYPKALALLEQAGEIEQLFRHDGGALRISASSTIGNYMLPEMIAHYRHDFPASPL
CCCCEEHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCE
ELNVGNSQDVIGAVADFRVDLGLIEGPCHMPELITLPWLEDELVVFAAPDNPLAGQPLTL
EEECCCCHHHHHHHHHHHHEEEEECCCCCCCCEEECCCCCCCEEEEECCCCCCCCCCEEE
EMLATAPWILREGGSGTREVLHHLLLAHLPHFQLVMELGNSEAIKHAVRHGMGISCLSRR
HHHHCCCHHEECCCCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCCCHHHHHHH
VIGEQLASGVLVELNIPLPPLMRTLYLIHHRQKHISNALQRFLGYCGFQPQ
HHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MHITLRQLEVFTEVLKSGSTTQASVVLALSQSAVSAALADLEGQLGVQLFDRVGKRLVIN
CCCHHHHHHHHHHHHHCCCCCHHHEEEEHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEE
EHGRLLYPKALALLEQAGEIEQLFRHDGGALRISASSTIGNYMLPEMIAHYRHDFPASPL
CCCCEEHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCE
ELNVGNSQDVIGAVADFRVDLGLIEGPCHMPELITLPWLEDELVVFAAPDNPLAGQPLTL
EEECCCCHHHHHHHHHHHHEEEEECCCCCCCCEEECCCCCCCEEEEECCCCCCCCCCEEE
EMLATAPWILREGGSGTREVLHHLLLAHLPHFQLVMELGNSEAIKHAVRHGMGISCLSRR
HHHHCCCHHEECCCCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCCCHHHHHHH
VIGEQLASGVLVELNIPLPPLMRTLYLIHHRQKHISNALQRFLGYCGFQPQ
HHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]