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The map label for this gene is fruA [H]

Identifier: 157371465

GI number: 157371465

Start: 3558602

End: 3560287

Strand: Reverse

Name: fruA [H]

Synonym: Spro_3228

Alternate gene names: 157371465

Gene position: 3560287-3558602 (Counterclockwise)

Preceding gene: 157371466

Following gene: 157371462

Centisome position: 65.34

GC content: 60.97

Gene sequence:

>1686_bases
ATGAAAACACTGCTGATGATAGACAGTTCGCTGGGGCAGGCGCGTGGTCACCTGGCGAAACGCATGCTCGGCGCTGCTGC
AGCGAAGGCCGGCCTGACGCTGGTGGAGTCCCCGGATGAGGCTGAACTGGTGGTGGTGGCGGGGCAATCTGCGCCTGCTG
ACGCCGCGCTGAACGGCAAGCGGGTTTATGTGGGCGATGTGGAACACGCGGTGCGTGAGCCGGAAGCCTTCCTGACGCAG
GCCATAGCCGAGGCAAAAGCCTGGCAGGCACCGGCAACGGCTGAACCGGTCAAAGCGGCGGGTCAAAAACGCATTGTGGC
GATCACCGCTTGTCCAACCGGTGTGGCGCATACCTTTATGGCGGCCGAGGCGATTGAGAGCGAAGCCAAAAAACGCGGCT
GGTGGGTGAAGGTTGAAACCCGCGGTTCCGTGGGTGCCGGTAATGCCATTACGCCAGAAGAAGTGGCGGCGGCGGATCTG
GTGATTGTGGCGGCCGATATCGAAGTGGATCTGGAGAAATTTGCCGGCAAGCCAATGTATCGCACCTCTACCGGGCTGGC
GTTAAAGAAAACCGCGCAGGAACTGGACAAGGCGCAGGCCGAAGCCGAGATTTTCCAGCCGCAAAAAAGCGGTGCTCCGG
CCGCGGCCGGCAAGAAGAAAGAGAGTGCCGGGCCTTATCGGCATCTGTTGACCGGCGTGTCTTATATGCTGCCGATGGTG
GTGGCGGGCGGTCTGTGTATCGCACTGTCGTTCGTATTCGGTATCAAGGCGTTCGAAGTCAAAGGCACGCTGGCGGCGGC
ACTGATGCAGATCGGTGGGGGTTCGGCCTTTGCACTGATGGTGCCGGTGCTGGCTGGTTTTATTGCTTTCTCAATTGCTG
ACCGTCCGGGCCTGACGCCGGGGTTGATTGGCGGCATGTTGGCAGTAAGCACCGGAGCCGGTTTCCTCGGCGGTATCATT
GCCGGTTTCCTGGCGGGTTATGTCGCCAAATTTATCAGCACCAAACTGCGTTTGCCGCAGAGTATGGAAGCGCTGAAGCC
GATCCTGATCATTCCGCTGGTTGCCAGCCTGATCGTGGGTCTGATCATGATCTTCATCGTCGGTACGCCGGTGGCGAAAA
TCATGGAAGGTCTGACCCATTGGTTGCAGTCGCTGGGTACCGCGAATGCGGTGCTGCTGGGCGCAATCCTCGGTGCCATG
ATGTGTACCGATATGGGTGGCCCGGTCAACAAGGCGGCCTATGCTTTTGGCGTGGCGTTGCTGAGTTCTTCGGTGTATGC
GCCGATGGCGGCGATTATGGCTGCCGGTATGGTGCCACCGCTGGCGATGGGGTTGGCAACGCTGCTGGCGCGTCGCAAGT
TCGACAAGTCACAACAGGAAGGCGGGAAAGCGGCGCTGGTGTTGGGCCTGTGCTTTATCTCCGAAGGCGCGATCCCGTTC
GCTGCCCGTGACCCGATGCGCGTGCTGCCATGCTGCATCGCTGGTGGCGCGCTGACCGGTGCGCTGTCCATGGCGTTTGG
TGCCAAACTGATGGCGCCGCACGGCGGGTTGTTCGTGCTGCTGATCCCGGGCGCAATCACTCCGGTACTGCTGTATCTGG
TGGCGATTATCGCCGGTACGCTCTTGGCGGGCGTGTCTTACGCACTGCTGAAACGTGCTGACGTCCCGGCGGCCAGCGTG
GCTTAA

Upstream 100 bases:

>100_bases
CCGTCAGCCAGAGCAACGTTGGCGTGACCGACCGTCCACAGTTGGCCGCGATGATGGCGCGTGTCGACCTGAAACCTTTT
AATCAATAGCGGGAGGCAGA

Downstream 100 bases:

>100_bases
GATTAGCGTTGGTAATAAAAAAACCGGAGTCCAGACTCTAATGCTTGTCAGTTAAGGTGATATTGTTCCCTCTCCTTTGG
GAGAGGGTTAGGGTGAGGGG

Product: PTS system fructose-specific transporter subunits IIBC

Products: NA

Alternate protein names: EIIBC-Fru; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MKTLLMIDSSLGQARGHLAKRMLGAAAAKAGLTLVESPDEAELVVVAGQSAPADAALNGKRVYVGDVEHAVREPEAFLTQ
AIAEAKAWQAPATAEPVKAAGQKRIVAITACPTGVAHTFMAAEAIESEAKKRGWWVKVETRGSVGAGNAITPEEVAAADL
VIVAADIEVDLEKFAGKPMYRTSTGLALKKTAQELDKAQAEAEIFQPQKSGAPAAAGKKKESAGPYRHLLTGVSYMLPMV
VAGGLCIALSFVFGIKAFEVKGTLAAALMQIGGGSAFALMVPVLAGFIAFSIADRPGLTPGLIGGMLAVSTGAGFLGGII
AGFLAGYVAKFISTKLRLPQSMEALKPILIIPLVASLIVGLIMIFIVGTPVAKIMEGLTHWLQSLGTANAVLLGAILGAM
MCTDMGGPVNKAAYAFGVALLSSSVYAPMAAIMAAGMVPPLAMGLATLLARRKFDKSQQEGGKAALVLGLCFISEGAIPF
AARDPMRVLPCCIAGGALTGALSMAFGAKLMAPHGGLFVLLIPGAITPVLLYLVAIIAGTLLAGVSYALLKRADVPAASV
A

Sequences:

>Translated_561_residues
MKTLLMIDSSLGQARGHLAKRMLGAAAAKAGLTLVESPDEAELVVVAGQSAPADAALNGKRVYVGDVEHAVREPEAFLTQ
AIAEAKAWQAPATAEPVKAAGQKRIVAITACPTGVAHTFMAAEAIESEAKKRGWWVKVETRGSVGAGNAITPEEVAAADL
VIVAADIEVDLEKFAGKPMYRTSTGLALKKTAQELDKAQAEAEIFQPQKSGAPAAAGKKKESAGPYRHLLTGVSYMLPMV
VAGGLCIALSFVFGIKAFEVKGTLAAALMQIGGGSAFALMVPVLAGFIAFSIADRPGLTPGLIGGMLAVSTGAGFLGGII
AGFLAGYVAKFISTKLRLPQSMEALKPILIIPLVASLIVGLIMIFIVGTPVAKIMEGLTHWLQSLGTANAVLLGAILGAM
MCTDMGGPVNKAAYAFGVALLSSSVYAPMAAIMAAGMVPPLAMGLATLLARRKFDKSQQEGGKAALVLGLCFISEGAIPF
AARDPMRVLPCCIAGGALTGALSMAFGAKLMAPHGGLFVLLIPGAITPVLLYLVAIIAGTLLAGVSYALLKRADVPAASV
A
>Mature_561_residues
MKTLLMIDSSLGQARGHLAKRMLGAAAAKAGLTLVESPDEAELVVVAGQSAPADAALNGKRVYVGDVEHAVREPEAFLTQ
AIAEAKAWQAPATAEPVKAAGQKRIVAITACPTGVAHTFMAAEAIESEAKKRGWWVKVETRGSVGAGNAITPEEVAAADL
VIVAADIEVDLEKFAGKPMYRTSTGLALKKTAQELDKAQAEAEIFQPQKSGAPAAAGKKKESAGPYRHLLTGVSYMLPMV
VAGGLCIALSFVFGIKAFEVKGTLAAALMQIGGGSAFALMVPVLAGFIAFSIADRPGLTPGLIGGMLAVSTGAGFLGGII
AGFLAGYVAKFISTKLRLPQSMEALKPILIIPLVASLIVGLIMIFIVGTPVAKIMEGLTHWLQSLGTANAVLLGAILGAM
MCTDMGGPVNKAAYAFGVALLSSSVYAPMAAIMAAGMVPPLAMGLATLLARRKFDKSQQEGGKAALVLGLCFISEGAIPF
AARDPMRVLPCCIAGGALTGALSMAFGAKLMAPHGGLFVLLIPGAITPVLLYLVAIIAGTLLAGVSYALLKRADVPAASV
A

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=562, Percent_Identity=79.0035587188612, Blast_Score=875, Evalue=0.0,
Organism=Escherichia coli, GI1786951, Length=427, Percent_Identity=37.2365339578454, Blast_Score=260, Evalue=2e-70,
Organism=Escherichia coli, GI87082348, Length=448, Percent_Identity=35.4910714285714, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI1790386, Length=301, Percent_Identity=44.5182724252492, Blast_Score=213, Evalue=4e-56,
Organism=Escherichia coli, GI1788729, Length=361, Percent_Identity=26.3157894736842, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1790387, Length=88, Percent_Identity=55.6818181818182, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1788730, Length=92, Percent_Identity=41.304347826087, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790390, Length=65, Percent_Identity=43.0769230769231, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 57337; Mature: 57337

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLLMIDSSLGQARGHLAKRMLGAAAAKAGLTLVESPDEAELVVVAGQSAPADAALNGK
CCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCCCCCCCCCC
RVYVGDVEHAVREPEAFLTQAIAEAKAWQAPATAEPVKAAGQKRIVAITACPTGVAHTFM
EEEECCHHHHHHCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEEEECCCHHHHHHH
AAEAIESEAKKRGWWVKVETRGSVGAGNAITPEEVAAADLVIVAADIEVDLEKFAGKPMY
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHCEEEEEECCCHHHHHHCCCCCE
RTSTGLALKKTAQELDKAQAEAEIFQPQKSGAPAAAGKKKESAGPYRHLLTGVSYMLPMV
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VAGGLCIALSFVFGIKAFEVKGTLAAALMQIGGGSAFALMVPVLAGFIAFSIADRPGLTP
HHHHHHHHHHHHHCCHHEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCH
GLIGGMLAVSTGAGFLGGIIAGFLAGYVAKFISTKLRLPQSMEALKPILIIPLVASLIVG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LIMIFIVGTPVAKIMEGLTHWLQSLGTANAVLLGAILGAMMCTDMGGPVNKAAYAFGVAL
HHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LSSSVYAPMAAIMAAGMVPPLAMGLATLLARRKFDKSQQEGGKAALVLGLCFISEGAIPF
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHCCCCCC
AARDPMRVLPCCIAGGALTGALSMAFGAKLMAPHGGLFVLLIPGAITPVLLYLVAIIAGT
CCCCCHHHHHHHHHCHHHHHHHHHHHCCHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHH
LLAGVSYALLKRADVPAASVA
HHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure
MKTLLMIDSSLGQARGHLAKRMLGAAAAKAGLTLVESPDEAELVVVAGQSAPADAALNGK
CCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCCCCCCCCCC
RVYVGDVEHAVREPEAFLTQAIAEAKAWQAPATAEPVKAAGQKRIVAITACPTGVAHTFM
EEEECCHHHHHHCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEEEECCCHHHHHHH
AAEAIESEAKKRGWWVKVETRGSVGAGNAITPEEVAAADLVIVAADIEVDLEKFAGKPMY
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHCEEEEEECCCHHHHHHCCCCCE
RTSTGLALKKTAQELDKAQAEAEIFQPQKSGAPAAAGKKKESAGPYRHLLTGVSYMLPMV
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VAGGLCIALSFVFGIKAFEVKGTLAAALMQIGGGSAFALMVPVLAGFIAFSIADRPGLTP
HHHHHHHHHHHHHCCHHEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCH
GLIGGMLAVSTGAGFLGGIIAGFLAGYVAKFISTKLRLPQSMEALKPILIIPLVASLIVG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LIMIFIVGTPVAKIMEGLTHWLQSLGTANAVLLGAILGAMMCTDMGGPVNKAAYAFGVAL
HHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LSSSVYAPMAAIMAAGMVPPLAMGLATLLARRKFDKSQQEGGKAALVLGLCFISEGAIPF
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHCCCCCC
AARDPMRVLPCCIAGGALTGALSMAFGAKLMAPHGGLFVLLIPGAITPVLLYLVAIIAGT
CCCCCHHHHHHHHHCHHHHHHHHHHHCCHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHH
LLAGVSYALLKRADVPAASVA
HHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3076173; 9278503; 9097040; 1981619 [H]