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The map label for this gene is azoR [H]

Identifier: 157370836

GI number: 157370836

Start: 2857436

End: 2858041

Strand: Direct

Name: azoR [H]

Synonym: Spro_2596

Alternate gene names: 157370836

Gene position: 2857436-2858041 (Clockwise)

Preceding gene: 157370834

Following gene: 157370840

Centisome position: 52.44

GC content: 57.26

Gene sequence:

>606_bases
ATGAGCAAAGTATTGGTTCTTAAATCAAGCATCCTGGCAGGTTACTCTCAGTCTAACCAACTGGCCGATTTCTTCGTTGA
GCAATGGGCAAGCGCTCATGGCAACGACACTATCACCGTACGTGACCTGGCTGCACAGCCAATCCCGGTACTGGACGGTG
AGCTGGTGGGTGCACTGCGCCCTTCCGATACGCCGCTGACCCCACGTCAGACCGAAGCGCTGGCCTTGTCCGATGAACTG
ATCGCTGAACTGCAGGCCAATGACACCATCGTTATGGCGGCACCGATGTACAACTTCAACATCCCGACCCAGTTGAAAAA
CTATTTCGACCTGATTGCCCGTGCCGGCGTCACCTTCCGTTACACCGAAAACGGCCCTGAAGGCCTGGTGAAAGGCAAAC
GCGCCATCATCCTGACCAGCCGCGGCGGCATTCATAAAGGCACCCCGACTGACCTGGTTGAACCTTACCTGCGTCTGTTC
CTGGGCTTTATCGGCATCACCGACGTTGAGTTCGTGTTTGCGGAAGGCATCGCTTACGGCCCGGACGTCGCGACCAAAGC
ACAGGCCGACGCCAAGGCCGTGCTGACTCAGGTGGTTGCCGCCTGA

Upstream 100 bases:

>100_bases
GAAATATTGCGCTATCACTGCTGGAAAAATGTGAATAGAGTGTACTGCATGCAAGGGACGCACCGTCCTGATGTCACTGT
CTTAACCTAAGGAATTACCG

Downstream 100 bases:

>100_bases
TGTCCCTCGCTGGGGCGTAATGCTTGTAAGTTAAGGTGTATTGTTCCCTCTCCTTTGAGAGAGGGTTAGGGTGAGGGGAT
ACATACCAACACGAAAGCCC

Product: azoreductase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase; FMN-dependent NADH-azo compound oxidoreductase [H]

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MSKVLVLKSSILAGYSQSNQLADFFVEQWASAHGNDTITVRDLAAQPIPVLDGELVGALRPSDTPLTPRQTEALALSDEL
IAELQANDTIVMAAPMYNFNIPTQLKNYFDLIARAGVTFRYTENGPEGLVKGKRAIILTSRGGIHKGTPTDLVEPYLRLF
LGFIGITDVEFVFAEGIAYGPDVATKAQADAKAVLTQVVAA

Sequences:

>Translated_201_residues
MSKVLVLKSSILAGYSQSNQLADFFVEQWASAHGNDTITVRDLAAQPIPVLDGELVGALRPSDTPLTPRQTEALALSDEL
IAELQANDTIVMAAPMYNFNIPTQLKNYFDLIARAGVTFRYTENGPEGLVKGKRAIILTSRGGIHKGTPTDLVEPYLRLF
LGFIGITDVEFVFAEGIAYGPDVATKAQADAKAVLTQVVAA
>Mature_200_residues
SKVLVLKSSILAGYSQSNQLADFFVEQWASAHGNDTITVRDLAAQPIPVLDGELVGALRPSDTPLTPRQTEALALSDELI
AELQANDTIVMAAPMYNFNIPTQLKNYFDLIARAGVTFRYTENGPEGLVKGKRAIILTSRGGIHKGTPTDLVEPYLRLFL
GFIGITDVEFVFAEGIAYGPDVATKAQADAKAVLTQVVAA

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=201, Percent_Identity=81.592039800995, Blast_Score=342, Evalue=7e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 21623; Mature: 21492

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVLVLKSSILAGYSQSNQLADFFVEQWASAHGNDTITVRDLAAQPIPVLDGELVGALR
CCCEEEEHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEECCC
PSDTPLTPRQTEALALSDELIAELQANDTIVMAAPMYNFNIPTQLKNYFDLIARAGVTFR
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEE
YTENGPEGLVKGKRAIILTSRGGIHKGTPTDLVEPYLRLFLGFIGITDVEFVFAEGIAYG
ECCCCCCHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCC
PDVATKAQADAKAVLTQVVAA
CCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKVLVLKSSILAGYSQSNQLADFFVEQWASAHGNDTITVRDLAAQPIPVLDGELVGALR
CCEEEEHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEECCC
PSDTPLTPRQTEALALSDELIAELQANDTIVMAAPMYNFNIPTQLKNYFDLIARAGVTFR
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEE
YTENGPEGLVKGKRAIILTSRGGIHKGTPTDLVEPYLRLFLGFIGITDVEFVFAEGIAYG
ECCCCCCHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCC
PDVATKAQADAKAVLTQVVAA
CCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA