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The map label for this gene is ldhA [H]

Identifier: 157370840

GI number: 157370840

Start: 2861949

End: 2862941

Strand: Direct

Name: ldhA [H]

Synonym: Spro_2600

Alternate gene names: 157370840

Gene position: 2861949-2862941 (Clockwise)

Preceding gene: 157370836

Following gene: 157370841

Centisome position: 52.52

GC content: 53.27

Gene sequence:

>993_bases
ATGAAAATGGCGATATACAGCACTAAACAGTATGACCGTAAATATCTCGAACTGGTGAATCAGCAGTTTGGCTACGAGCT
GGAGTTTTTCGATTTCCTGCTCAGTAAAAGAACCGCCAAAAACGCCGCCGGTTGCAAAGCCATCTGTATCTTCGTTAACG
ACGACGGCAGCCGTGAAGTCCTTGAAGAACTGGCGGCGCTGGGGGTGGAAATCCTGGCCCTGCGCTGCGCCGGTTTCAAT
AACGTGGATCTGGACGCCGCCAAAGAATTGGGCATCAAAGTGGTACGCGTCCCGGCCTATTCGCCGGAAGCGGTCGCTGA
ACACGCCGTGGGTATGATGATGTGCCTGAATCGTCGTATTCATCGCGCTTATCAGCGTACCCGCGATGCCAACTTCTCGC
TCGAGGGGTTGATTGGCTTCAATATGCATAACCGCACCGCCGGGGTGATTGGCACCGGTAAAATTGGCATCGCCACCATG
CGGATCCTGAAAGGGTTTGGCATGAAACTATTGGCTTATGATCCCTACCCAAGCGAACAAGCGCTGGAGATGGGTGCCGA
ATATGTCGATCTGAAAACGCTGTACGCACAGTCCGACGTGATCACCCTGCACTGCCCGTTGACGCCGGAAAACCACCATC
TGCTGGACAGTGATGCTTTCAGCCTGATGAAAAACGGCGTGATGATTATCAACACCAGCCGTGGCGCACTGATTGACTCC
TCGGCGGCGATTGATGCCCTGAAGCAGCAGAAAATCGGCGCACTGGGAATGGATGTGTATGAGAATGAACGCGATCTGTT
CTTTGAAGACAAATCCAATGACGTGATCCAGGACGATATCTTCCGCCGCCTGTCAGCCTGCCACAACGTGCTGTTTACCG
GCCATCAGGCTTTCCTGACCGAAGAGGCGCTGACCAGCATCTCGGAAACCACGCTGAAAAACATTCACCAATTGGAACAG
GGCGAGAACTGCCCGAATCAGCTTAATGCCTGA

Upstream 100 bases:

>100_bases
GTAGTTACCCCTTTTTAACTTTACCGAAGCCGGCTAACCAATTGTAGAATTTACTAAAATCTGTTAAATTGATCACAAAA
ATGACGCTGGAGAAACCACG

Downstream 100 bases:

>100_bases
TATTTTTAACAAGGAAAGTCGATGAAAAAGTCTATCTCAGTGGCGTTAACGGCGCTGCTGTTAGCGGGGTGTGGTATGAG
CCAGAACGGTAAAACGGTGA

Product: NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: D-LDH; Fermentative lactate dehydrogenase [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKMAIYSTKQYDRKYLELVNQQFGYELEFFDFLLSKRTAKNAAGCKAICIFVNDDGSREVLEELAALGVEILALRCAGFN
NVDLDAAKELGIKVVRVPAYSPEAVAEHAVGMMMCLNRRIHRAYQRTRDANFSLEGLIGFNMHNRTAGVIGTGKIGIATM
RILKGFGMKLLAYDPYPSEQALEMGAEYVDLKTLYAQSDVITLHCPLTPENHHLLDSDAFSLMKNGVMIINTSRGALIDS
SAAIDALKQQKIGALGMDVYENERDLFFEDKSNDVIQDDIFRRLSACHNVLFTGHQAFLTEEALTSISETTLKNIHQLEQ
GENCPNQLNA

Sequences:

>Translated_330_residues
MKMAIYSTKQYDRKYLELVNQQFGYELEFFDFLLSKRTAKNAAGCKAICIFVNDDGSREVLEELAALGVEILALRCAGFN
NVDLDAAKELGIKVVRVPAYSPEAVAEHAVGMMMCLNRRIHRAYQRTRDANFSLEGLIGFNMHNRTAGVIGTGKIGIATM
RILKGFGMKLLAYDPYPSEQALEMGAEYVDLKTLYAQSDVITLHCPLTPENHHLLDSDAFSLMKNGVMIINTSRGALIDS
SAAIDALKQQKIGALGMDVYENERDLFFEDKSNDVIQDDIFRRLSACHNVLFTGHQAFLTEEALTSISETTLKNIHQLEQ
GENCPNQLNA
>Mature_330_residues
MKMAIYSTKQYDRKYLELVNQQFGYELEFFDFLLSKRTAKNAAGCKAICIFVNDDGSREVLEELAALGVEILALRCAGFN
NVDLDAAKELGIKVVRVPAYSPEAVAEHAVGMMMCLNRRIHRAYQRTRDANFSLEGLIGFNMHNRTAGVIGTGKIGIATM
RILKGFGMKLLAYDPYPSEQALEMGAEYVDLKTLYAQSDVITLHCPLTPENHHLLDSDAFSLMKNGVMIINTSRGALIDS
SAAIDALKQQKIGALGMDVYENERDLFFEDKSNDVIQDDIFRRLSACHNVLFTGHQAFLTEEALTSISETTLKNIHQLEQ
GENCPNQLNA

Specific function: Fermentative lactate dehydrogenase [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=212, Percent_Identity=32.5471698113208, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI61743967, Length=288, Percent_Identity=32.6388888888889, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI4557497, Length=288, Percent_Identity=32.6388888888889, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI145580578, Length=287, Percent_Identity=30.6620209059233, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI4557499, Length=287, Percent_Identity=30.6620209059233, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI145580575, Length=287, Percent_Identity=30.6620209059233, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI6912396, Length=270, Percent_Identity=29.2592592592593, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI1787645, Length=328, Percent_Identity=79.8780487804878, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI87082289, Length=191, Percent_Identity=33.5078534031414, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789279, Length=248, Percent_Identity=31.0483870967742, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788660, Length=210, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25147481, Length=277, Percent_Identity=28.5198555956679, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17532191, Length=193, Percent_Identity=29.0155440414508, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320925, Length=310, Percent_Identity=29.6774193548387, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324055, Length=213, Percent_Identity=33.8028169014084, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6322116, Length=232, Percent_Identity=31.8965517241379, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=209, Percent_Identity=27.2727272727273, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324980, Length=145, Percent_Identity=26.8965517241379, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24646446, Length=281, Percent_Identity=32.3843416370107, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24646448, Length=281, Percent_Identity=32.3843416370107, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24646452, Length=281, Percent_Identity=32.3843416370107, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24646450, Length=281, Percent_Identity=32.3843416370107, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI62472511, Length=281, Percent_Identity=32.3843416370107, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19921140, Length=233, Percent_Identity=26.6094420600858, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28574286, Length=277, Percent_Identity=29.2418772563177, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI45552429, Length=210, Percent_Identity=30, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585514, Length=210, Percent_Identity=30, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI28574282, Length=210, Percent_Identity=30, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI28574284, Length=210, Percent_Identity=30, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI45551003, Length=210, Percent_Identity=30, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI28571528, Length=278, Percent_Identity=26.2589928057554, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585516, Length=199, Percent_Identity=27.6381909547739, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 36774; Mature: 36774

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMAIYSTKQYDRKYLELVNQQFGYELEFFDFLLSKRTAKNAAGCKAICIFVNDDGSREV
CCEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHH
LEELAALGVEILALRCAGFNNVDLDAAKELGIKVVRVPAYSPEAVAEHAVGMMMCLNRRI
HHHHHHCCHHHHHHHHCCCCCCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
HRAYQRTRDANFSLEGLIGFNMHNRTAGVIGTGKIGIATMRILKGFGMKLLAYDPYPSEQ
HHHHHHHCCCCCEEEEEEEECCCCCCCCEEECCCHHHHHHHHHHHCCCEEEEECCCCCHH
ALEMGAEYVDLKTLYAQSDVITLHCPLTPENHHLLDSDAFSLMKNGVMIINTSRGALIDS
HHHCCCHHHHHHHHEECCCEEEEECCCCCCCCCCCCHHHHHHHHCCEEEEECCCCCEEEC
SAAIDALKQQKIGALGMDVYENERDLFFEDKSNDVIQDDIFRRLSACHNVLFTGHQAFLT
HHHHHHHHHHHCCCCCCHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
EEALTSISETTLKNIHQLEQGENCPNQLNA
HHHHHHHHHHHHHHHHHHHCCCCCCHHCCC
>Mature Secondary Structure
MKMAIYSTKQYDRKYLELVNQQFGYELEFFDFLLSKRTAKNAAGCKAICIFVNDDGSREV
CCEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHH
LEELAALGVEILALRCAGFNNVDLDAAKELGIKVVRVPAYSPEAVAEHAVGMMMCLNRRI
HHHHHHCCHHHHHHHHCCCCCCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
HRAYQRTRDANFSLEGLIGFNMHNRTAGVIGTGKIGIATMRILKGFGMKLLAYDPYPSEQ
HHHHHHHCCCCCEEEEEEEECCCCCCCCEEECCCHHHHHHHHHHHCCCEEEEECCCCCHH
ALEMGAEYVDLKTLYAQSDVITLHCPLTPENHHLLDSDAFSLMKNGVMIINTSRGALIDS
HHHCCCHHHHHHHHEECCCEEEEECCCCCCCCCCCCHHHHHHHHCCEEEEECCCCCEEEC
SAAIDALKQQKIGALGMDVYENERDLFFEDKSNDVIQDDIFRRLSACHNVLFTGHQAFLT
HHHHHHHHHHHCCCCCCHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
EEALTSISETTLKNIHQLEQGENCPNQLNA
HHHHHHHHHHHHHHHHHHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9025293; 9097039; 9278503; 8349564 [H]