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The map label for this gene is opdE [H]

Identifier: 157370696

GI number: 157370696

Start: 2690264

End: 2691460

Strand: Direct

Name: opdE [H]

Synonym: Spro_2456

Alternate gene names: 157370696

Gene position: 2690264-2691460 (Clockwise)

Preceding gene: 157370694

Following gene: 157370697

Centisome position: 49.37

GC content: 52.88

Gene sequence:

>1197_bases
ATGCCCAGATCGTCGAGTGACACACCACTTCACAATGAGCGCGCGTACTGGAGTGGCGTATTCGCCATGACATTGTGCGT
CTTCACCTTAATTGCTTCGGAATTTATGCCAGTCAGCCTACTGACACCAATATCCGCGGACCTGGCCGTGAGTGAGGGAC
TGGTTGGTCAGGGGATCGCTATTTCCGGACTTTTTGCCGTATTGACGAGTCTCTCCATTTCTACGCTGGCTGGGGCTGCG
AATCGCAAAATCTTGCTGTTGGGTATGACGGCCTTGATGGCGCTTTCCGGAGCCATCATAGCGTTAGCGCCCAGCTATTT
TATTTACATGGCTGGCCGAGCCCTGATTGGAATTGCTATTGGTGGCTTCTGGTCACTATCGGCTGCCACGGCTATTCGCC
TGGTTCCGCAACATCGGGTACCTCGCGCACTGGCTATTTTTAATGGGGGCAACGCGCTGGCCACTGTTATAGCCGCTCCA
TTGGGGAGTTATCTGGGTTCTGTCATCGGCTGGCGTGGCGCATTCTTTTGCATTGTGCCGGTCGCGGTAATTGCGTTTAT
TTGGCAGTTGTTCAGCTTGCCGGCCATGACTACGGATAAAGACGCCAAGCGTAGCGGCACTGTATTTCGCCTTTTTGCCA
ATCCGTTGGTGACCCTTGGCCTTGCCGCCTGTGGATTGTTCTTTATGGGCCAGTTTTCTTTGTTTACCTATTTGCGTCCG
TTTCTTGAAACGGTAACACGGGTGGATATCACCACGCTGTCGCTCATTTTGCTGACCATCGGGGTTGCGGGCTTTATCGG
TACTTTGCTGATTGGCATTTTCTTGAAATCCACCTTCTATCTGACGTTGATCGCCATCCCATTGCTGATGGCTGCCATAG
CCGGAGCGCTGATCGCTTTTGGCCACAGCGTCTGGATTGTCACACTGCTGCTGGGTTTATGGGGGTTGTTCGCTACGGCT
GCTCCGACCGGTTGGTGGACCTGGATCGCCAGAACATTGCCTGATGATGCGGAAGCCGGCGGTGGGTTGATGGTTGCCAT
AGTGCAGCTGTCAATCGCACTGGGTTCCACCCTCGGTGGTTTCGCATTTGACCATAGCGGTTATCAAAGCACTTTCACCA
TCAGCATCGTTTTATTAGTGATCGCGGCTGCCCTGACTTTATGGACGTCACGTAAAGATCTCTACCAAACCAATTGA

Upstream 100 bases:

>100_bases
ATTTCATCTTTCATTCACCTTTTCTGACGTTATTCCTGCCAATAGCGTGCGGCCCCGGCTTGCCTAAACACAGGCGGGGA
TTATTCGGAGTCAGCAGTTT

Downstream 100 bases:

>100_bases
GGATTATTCAGTGAGAACATTAACCATGTTATTGGGGCTGTTAATCAGCTCGTTTGCTGCCACAGGAGCCGATATGTCTA
ACGGAGCAGATAACTTTTAC

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MPRSSSDTPLHNERAYWSGVFAMTLCVFTLIASEFMPVSLLTPISADLAVSEGLVGQGIAISGLFAVLTSLSISTLAGAA
NRKILLLGMTALMALSGAIIALAPSYFIYMAGRALIGIAIGGFWSLSAATAIRLVPQHRVPRALAIFNGGNALATVIAAP
LGSYLGSVIGWRGAFFCIVPVAVIAFIWQLFSLPAMTTDKDAKRSGTVFRLFANPLVTLGLAACGLFFMGQFSLFTYLRP
FLETVTRVDITTLSLILLTIGVAGFIGTLLIGIFLKSTFYLTLIAIPLLMAAIAGALIAFGHSVWIVTLLLGLWGLFATA
APTGWWTWIARTLPDDAEAGGGLMVAIVQLSIALGSTLGGFAFDHSGYQSTFTISIVLLVIAAALTLWTSRKDLYQTN

Sequences:

>Translated_398_residues
MPRSSSDTPLHNERAYWSGVFAMTLCVFTLIASEFMPVSLLTPISADLAVSEGLVGQGIAISGLFAVLTSLSISTLAGAA
NRKILLLGMTALMALSGAIIALAPSYFIYMAGRALIGIAIGGFWSLSAATAIRLVPQHRVPRALAIFNGGNALATVIAAP
LGSYLGSVIGWRGAFFCIVPVAVIAFIWQLFSLPAMTTDKDAKRSGTVFRLFANPLVTLGLAACGLFFMGQFSLFTYLRP
FLETVTRVDITTLSLILLTIGVAGFIGTLLIGIFLKSTFYLTLIAIPLLMAAIAGALIAFGHSVWIVTLLLGLWGLFATA
APTGWWTWIARTLPDDAEAGGGLMVAIVQLSIALGSTLGGFAFDHSGYQSTFTISIVLLVIAAALTLWTSRKDLYQTN
>Mature_397_residues
PRSSSDTPLHNERAYWSGVFAMTLCVFTLIASEFMPVSLLTPISADLAVSEGLVGQGIAISGLFAVLTSLSISTLAGAAN
RKILLLGMTALMALSGAIIALAPSYFIYMAGRALIGIAIGGFWSLSAATAIRLVPQHRVPRALAIFNGGNALATVIAAPL
GSYLGSVIGWRGAFFCIVPVAVIAFIWQLFSLPAMTTDKDAKRSGTVFRLFANPLVTLGLAACGLFFMGQFSLFTYLRPF
LETVTRVDITTLSLILLTIGVAGFIGTLLIGIFLKSTFYLTLIAIPLLMAAIAGALIAFGHSVWIVTLLLGLWGLFATAA
PTGWWTWIARTLPDDAEAGGGLMVAIVQLSIALGSTLGGFAFDHSGYQSTFTISIVLLVIAAALTLWTSRKDLYQTN

Specific function: Regulates the expression of oprD which encodes the imipenem-specific porin [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis ywfA [H]

Homologues:

Organism=Escherichia coli, GI145693198, Length=372, Percent_Identity=39.247311827957, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1787947, Length=276, Percent_Identity=27.1739130434783, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787808, Length=240, Percent_Identity=25.8333333333333, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1786595, Length=348, Percent_Identity=24.4252873563218, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42040; Mature: 41909

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRSSSDTPLHNERAYWSGVFAMTLCVFTLIASEFMPVSLLTPISADLAVSEGLVGQGIA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHCCCCCCCHH
ISGLFAVLTSLSISTLAGAANRKILLLGMTALMALSGAIIALAPSYFIYMAGRALIGIAI
HHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
GGFWSLSAATAIRLVPQHRVPRALAIFNGGNALATVIAAPLGSYLGSVIGWRGAFFCIVP
CCHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAVIAFIWQLFSLPAMTTDKDAKRSGTVFRLFANPLVTLGLAACGLFFMGQFSLFTYLRP
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLETVTRVDITTLSLILLTIGVAGFIGTLLIGIFLKSTFYLTLIAIPLLMAAIAGALIAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GHSVWIVTLLLGLWGLFATAAPTGWWTWIARTLPDDAEAGGGLMVAIVQLSIALGSTLGG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCC
FAFDHSGYQSTFTISIVLLVIAAALTLWTSRKDLYQTN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCC
>Mature Secondary Structure 
PRSSSDTPLHNERAYWSGVFAMTLCVFTLIASEFMPVSLLTPISADLAVSEGLVGQGIA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHCCCCCCCHH
ISGLFAVLTSLSISTLAGAANRKILLLGMTALMALSGAIIALAPSYFIYMAGRALIGIAI
HHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
GGFWSLSAATAIRLVPQHRVPRALAIFNGGNALATVIAAPLGSYLGSVIGWRGAFFCIVP
CCHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAVIAFIWQLFSLPAMTTDKDAKRSGTVFRLFANPLVTLGLAACGLFFMGQFSLFTYLRP
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLETVTRVDITTLSLILLTIGVAGFIGTLLIGIFLKSTFYLTLIAIPLLMAAIAGALIAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GHSVWIVTLLLGLWGLFATAAPTGWWTWIARTLPDDAEAGGGLMVAIVQLSIALGSTLGG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCC
FAFDHSGYQSTFTISIVLLVIAAALTLWTSRKDLYQTN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1427017; 10984043 [H]