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The map label for this gene is glnS

Identifier: 157369472

GI number: 157369472

Start: 1350279

End: 1351943

Strand: Direct

Name: glnS

Synonym: Spro_1229

Alternate gene names: 157369472

Gene position: 1350279-1351943 (Clockwise)

Preceding gene: 157369471

Following gene: 157369473

Centisome position: 24.78

GC content: 54.71

Gene sequence:

>1665_bases
ATGAGTGAGGCTGAAGCCCGCCCAACCAATTTTATCCGTCAGATCGTTGATGAAGATCTGGCGTCCGGGAAACACAAGTC
AGTCCATACCCGTTTCCCGCCGGAGCCAAATGGCTATCTGCATATCGGCCATGCGAAATCCATCTGCCTGAATTTCGGTA
TCGCTCGCGATTACCAGGGCCAATGCAACCTGCGTTTTGATGACACCAACCCGGTGAAAGAAGACATCGAGTTCGTTGAG
TCAATCAAACATGACGTCGAGTGGCTGGGCTTTGAGTGGAGCGGCAACGTTCGTTACTCCTCGGATTACTTCGATCAACT
GCACAACTATGCGGTGGAGCTGATCAACAAGGGCCTGGCTTACGTCGACGAACTGTCGCCTGAGCAAATCCGTGAATATC
GCGGCAGCCTGACTGCGCCGGGTAAAGACAGCCCGTACCGTGGCCGCAGCGTGGAAGAGAACCTGGCGCTGTTTGAAAAA
ATGCGTAACGGTGAATTCGCCGAAGGGACCGCTTGCCTGCGTGCCAAGATTGACATGGCTTCCTCGTTTATCGTGATGCG
CGATCCTGTGTTGTACCGTATCAAATTTGCAGAACACCACCAGACCGGCAATAAATGGTGCATCTACCCGATGTACGATT
TCACCCACTGCATTTCCGATGCGCTGGAAGGGATCACCCATTCGCTATGTACGCTGGAGTTCCAGGACAACCGCCGTCTG
TATGACTGGGTGCTGGATAACATCACCATCCCTTGTCATCCGCGTCAGTACGAGTTCTCGCGTCTGAATCTTGAGTACGC
CATCATGTCGAAGCGCAAGCTGAACCTGTTGGTGACCGAGAAAATCGTTGAGGGCTGGGATGACCCGCGTATGCCGACCG
TTTCCGGCCTGCGTCGCCGTGGTTATACCGCTGCGTCAATCCGCGAATTCTGCCAGCGTATCGGCGTGACCAAGCAGGAT
AACAACGTCGAAATGATGGCGCTTGAGGCCTGTATTCGTGAAGAGCTGAACGAGAACGCACCACGTGCGATGGCCGTGCT
GGATCCGGTGAAGGTAGTCATTGAAAACCTGACCACCGGCGTGGAAATGGTGACCATGCCTAACCACCCGAGCAAGCCGG
AAATGGGCAGCCGTGAAGTGCCATTCAGCCGTGATATTTATATCGATCGCGCCGACTTCCGTGAAGAAGCCAACAAGCAG
TACAAGCGCCTGGTGCTGGGTAAAGAAGTTCGCCTGCGCAATGCATACGTGATCAAGGCGGAACGCATTGAGAAAGACGC
TGAAGGTGAGATCACCACTATTTTCTGCAGCTACGATCCTGATACCTTGAGCAAGGATCCTGCCGATGGCCGCAAGGTGA
AAGGCGTGATCCACTGGGTGTCTGCCGAGCATGCGTTGCCGGCGGAAATTCGCGTGTATGACCGTCTGTTCAGCGTGCCA
AACCCGGCGGCGGCAGAGGACTTCCTCACCACCATTAACCCGGAGTCACTGGTTATCAAGCACGGCTTTGTTGAGCCAAG
TCTGGCGGCCGCACAGCCGGAAAAAGCCTACCAGTTCGAACGTGAAGGTTATTTCTGTGCTGACAACCGTCACTCTTCGG
CCGAGCATCTGGTGTTTAACCGTACCGTGGGCCTGCGCGATACCTGGGCTAAAATTGGCGCCTGA

Upstream 100 bases:

>100_bases
CAATGCTCCGCATTTTTCGTGAAACAAACGGTTGTTTCCGGGCGTGGCTTGTTGGAACATAGGCGGTTATGAGTAGCGCT
TTGCATTGAGGAATAGTGAA

Downstream 100 bases:

>100_bases
GTGCTGATCAAAATCTAATGCGATAAAGCGCAACTTCGGTTGCGCTTTTTTTTGTCCGCCAGCCCGGTTTTTCTCCCCGT
TAATGCGTTTAATTCCTCAA

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MSEAEARPTNFIRQIVDEDLASGKHKSVHTRFPPEPNGYLHIGHAKSICLNFGIARDYQGQCNLRFDDTNPVKEDIEFVE
SIKHDVEWLGFEWSGNVRYSSDYFDQLHNYAVELINKGLAYVDELSPEQIREYRGSLTAPGKDSPYRGRSVEENLALFEK
MRNGEFAEGTACLRAKIDMASSFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCISDALEGITHSLCTLEFQDNRRL
YDWVLDNITIPCHPRQYEFSRLNLEYAIMSKRKLNLLVTEKIVEGWDDPRMPTVSGLRRRGYTAASIREFCQRIGVTKQD
NNVEMMALEACIREELNENAPRAMAVLDPVKVVIENLTTGVEMVTMPNHPSKPEMGSREVPFSRDIYIDRADFREEANKQ
YKRLVLGKEVRLRNAYVIKAERIEKDAEGEITTIFCSYDPDTLSKDPADGRKVKGVIHWVSAEHALPAEIRVYDRLFSVP
NPAAAEDFLTTINPESLVIKHGFVEPSLAAAQPEKAYQFEREGYFCADNRHSSAEHLVFNRTVGLRDTWAKIGA

Sequences:

>Translated_554_residues
MSEAEARPTNFIRQIVDEDLASGKHKSVHTRFPPEPNGYLHIGHAKSICLNFGIARDYQGQCNLRFDDTNPVKEDIEFVE
SIKHDVEWLGFEWSGNVRYSSDYFDQLHNYAVELINKGLAYVDELSPEQIREYRGSLTAPGKDSPYRGRSVEENLALFEK
MRNGEFAEGTACLRAKIDMASSFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCISDALEGITHSLCTLEFQDNRRL
YDWVLDNITIPCHPRQYEFSRLNLEYAIMSKRKLNLLVTEKIVEGWDDPRMPTVSGLRRRGYTAASIREFCQRIGVTKQD
NNVEMMALEACIREELNENAPRAMAVLDPVKVVIENLTTGVEMVTMPNHPSKPEMGSREVPFSRDIYIDRADFREEANKQ
YKRLVLGKEVRLRNAYVIKAERIEKDAEGEITTIFCSYDPDTLSKDPADGRKVKGVIHWVSAEHALPAEIRVYDRLFSVP
NPAAAEDFLTTINPESLVIKHGFVEPSLAAAQPEKAYQFEREGYFCADNRHSSAEHLVFNRTVGLRDTWAKIGA
>Mature_553_residues
SEAEARPTNFIRQIVDEDLASGKHKSVHTRFPPEPNGYLHIGHAKSICLNFGIARDYQGQCNLRFDDTNPVKEDIEFVES
IKHDVEWLGFEWSGNVRYSSDYFDQLHNYAVELINKGLAYVDELSPEQIREYRGSLTAPGKDSPYRGRSVEENLALFEKM
RNGEFAEGTACLRAKIDMASSFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCISDALEGITHSLCTLEFQDNRRLY
DWVLDNITIPCHPRQYEFSRLNLEYAIMSKRKLNLLVTEKIVEGWDDPRMPTVSGLRRRGYTAASIREFCQRIGVTKQDN
NVEMMALEACIREELNENAPRAMAVLDPVKVVIENLTTGVEMVTMPNHPSKPEMGSREVPFSRDIYIDRADFREEANKQY
KRLVLGKEVRLRNAYVIKAERIEKDAEGEITTIFCSYDPDTLSKDPADGRKVKGVIHWVSAEHALPAEIRVYDRLFSVPN
PAAAEDFLTTINPESLVIKHGFVEPSLAAAQPEKAYQFEREGYFCADNRHSSAEHLVFNRTVGLRDTWAKIGA

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI4826960, Length=532, Percent_Identity=46.4285714285714, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI62241042, Length=516, Percent_Identity=35.077519379845, Blast_Score=298, Evalue=1e-80,
Organism=Escherichia coli, GI1786895, Length=553, Percent_Identity=87.3417721518987, Blast_Score=1028, Evalue=0.0,
Organism=Escherichia coli, GI1788743, Length=122, Percent_Identity=31.9672131147541, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17543332, Length=535, Percent_Identity=43.7383177570093, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17511007, Length=529, Percent_Identity=33.648393194707, Blast_Score=267, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6324742, Length=564, Percent_Identity=41.6666666666667, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321192, Length=528, Percent_Identity=35.0378787878788, Blast_Score=304, Evalue=2e-83,
Organism=Drosophila melanogaster, GI17864488, Length=550, Percent_Identity=44.1818181818182, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24649466, Length=514, Percent_Identity=35.2140077821012, Blast_Score=275, Evalue=7e-74,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYQ_SERP5 (A8GB43)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477461.1
- ProteinModelPortal:   A8GB43
- SMR:   A8GB43
- STRING:   A8GB43
- GeneID:   5603593
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1229
- eggNOG:   COG0008
- HOGENOM:   HBG334108
- OMA:   RMPTIAG
- ProtClustDB:   PRK05347
- BioCyc:   SPRO399741:SPRO_1229-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00126
- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:2.40.240.10
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119:SF3
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00440

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C; SSF50715 Ribosomal_L25rel

EC number: =6.1.1.18

Molecular weight: Translated: 63508; Mature: 63376

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 271-271

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAEARPTNFIRQIVDEDLASGKHKSVHTRFPPEPNGYLHIGHAKSICLNFGIARDYQG
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCHHHHHHCCCCCCCCCC
QCNLRFDDTNPVKEDIEFVESIKHDVEWLGFEWSGNVRYSSDYFDQLHNYAVELINKGLA
CEEEEECCCCCHHHHHHHHHHHHHHHHEEEEEECCCEEECHHHHHHHHHHHHHHHHCCHH
YVDELSPEQIREYRGSLTAPGKDSPYRGRSVEENLALFEKMRNGEFAEGTACLRAKIDMA
HHHCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHEEHHHH
SSFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCISDALEGITHSLCTLEFQDNRRL
CCEEEEECCEEEEEEEHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHEEEEECCCCEE
YDWVLDNITIPCHPRQYEFSRLNLEYAIMSKRKLNLLVTEKIVEGWDDPRMPTVSGLRRR
EHHHHHCEEECCCCCCCCHHHCCEEEEEECCCCCCCEEHHHHHCCCCCCCCCHHHHHHHC
GYTAASIREFCQRIGVTKQDNNVEMMALEACIREELNENAPRAMAVLDPVKVVIENLTTG
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
VEMVTMPNHPSKPEMGSREVPFSRDIYIDRADFREEANKQYKRLVLGKEVRLRNAYVIKA
EEEEECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEECCEEEEEH
ERIEKDAEGEITTIFCSYDPDTLSKDPADGRKVKGVIHWVSAEHALPAEIRVYDRLFSVP
HHCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCC
NPAAAEDFLTTINPESLVIKHGFVEPSLAAAQPEKAYQFEREGYFCADNRHSSAEHLVFN
CCCCHHHHHHHCCCCEEEEEECCCCCCHHHCCCHHHHHHHCCCEEEECCCCCCCCEEEEE
RTVGLRDTWAKIGA
CCCCCCHHHHHHCC
>Mature Secondary Structure 
SEAEARPTNFIRQIVDEDLASGKHKSVHTRFPPEPNGYLHIGHAKSICLNFGIARDYQG
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCHHHHHHCCCCCCCCCC
QCNLRFDDTNPVKEDIEFVESIKHDVEWLGFEWSGNVRYSSDYFDQLHNYAVELINKGLA
CEEEEECCCCCHHHHHHHHHHHHHHHHEEEEEECCCEEECHHHHHHHHHHHHHHHHCCHH
YVDELSPEQIREYRGSLTAPGKDSPYRGRSVEENLALFEKMRNGEFAEGTACLRAKIDMA
HHHCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHEEHHHH
SSFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCISDALEGITHSLCTLEFQDNRRL
CCEEEEECCEEEEEEEHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHEEEEECCCCEE
YDWVLDNITIPCHPRQYEFSRLNLEYAIMSKRKLNLLVTEKIVEGWDDPRMPTVSGLRRR
EHHHHHCEEECCCCCCCCHHHCCEEEEEECCCCCCCEEHHHHHCCCCCCCCCHHHHHHHC
GYTAASIREFCQRIGVTKQDNNVEMMALEACIREELNENAPRAMAVLDPVKVVIENLTTG
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
VEMVTMPNHPSKPEMGSREVPFSRDIYIDRADFREEANKQYKRLVLGKEVRLRNAYVIKA
EEEEECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEECCEEEEEH
ERIEKDAEGEITTIFCSYDPDTLSKDPADGRKVKGVIHWVSAEHALPAEIRVYDRLFSVP
HHCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCC
NPAAAEDFLTTINPESLVIKHGFVEPSLAAAQPEKAYQFEREGYFCADNRHSSAEHLVFN
CCCCHHHHHHHCCCCEEEEEECCCCCCHHHCCCHHHHHHHCCCEEEECCCCCCCCEEEEE
RTVGLRDTWAKIGA
CCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA