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The map label for this gene is nagE [H]

Identifier: 157369471

GI number: 157369471

Start: 1348091

End: 1350037

Strand: Direct

Name: nagE [H]

Synonym: Spro_1228

Alternate gene names: 157369471

Gene position: 1348091-1350037 (Clockwise)

Preceding gene: 157369464

Following gene: 157369472

Centisome position: 24.74

GC content: 56.39

Gene sequence:

>1947_bases
GTGAACATACTCGGCTTTTTCCAAAGATTGGGCAGGTCGTTACAACTGCCAATCGCAGTACTGCCCGTTGCGGCGCTTTT
GCTACGCTTCGGCCAGCCTGATTTACTGAATATTTCATTCATCGCACAGGCCGGTGGATCAATCTTCGATAACCTGGCGT
TAATCTTTGCTATCGGTGTGGCGGCAACCTGGTCGAAAGACAATGCCGGTTCAGCCGCATTGGCGGGCGCGGTGGGTTAC
TTCATCCTGACCAAGGCAATGGTCACCATTAACCCGGCGATCAATATGGGTGTGCTGGCGGGTATCATTACCGGCCTGGT
GGGCGGTGCGGTCTATAACCGCTGGTCCGGCATCAAACTGCCCGAGTTCCTCTCCTTCTTCGGCGGCAAGCGTTTCGTCC
CAATCGCTACCGGCTTTTTCTGTTTAATCCTTGCTGCCATCTTTGGTTATATCTGGCCACCGGTACAAAATGCGATCCAC
GCAGGCGGTGAATGGATTGTTTCGATGGGCGCTATCGGTTCCGGTATTTTCGGCTTCGTTAACCGTCTGCTGATCCCAAC
CGGTCTGCATCAGGTTCTGAACACCATTGCCTGGTTCCAGATTGGTGAGTTTACCAACGCTGCCGGTACCATCTTCCATG
GCGACATCAACCGCTTCTACGCGGGTGACGGTACTGCCGGGATGTTCATGTCAGGCTTCTTCCCGATCATGATGTTCGGT
TTGCCGGGTGCGGCATTGGCCATGTACTTCGCTGCGCCGAAAGAACGTCGCCCAATGGTTGGCGGTATGCTGCTGTCCGT
TGCGCTGACCGCATTCCTGACCGGTGTGACCGAGCCGCTTGAATTCCTGTTCATGTTCCTGGCTCCGCTGCTGTATCTGG
TCCACGCCATTTTGACCGGTATCAGCCTGTTTGTAGCCACCTTGCTGGGTATTCACGCCGGGTTCTCCTTCTCTGCAGGT
GCCATCGACTATGTGCTGATGTACAACCTGCCGGCTGCGAGCAAGAACGTCTGGATGCTGGTGTTGATGGGTCTGGTCGC
CTTCGCGGTGTACTTCGTCCTGTTCTCCGTCATTATCCGTGCATTCAACCTGAAAACCCCGGGCCGTGAAGATGCGTCCG
ACGATGTCGTCGCACCTGAAGCCAACAGCAATACCGATGAAGGCCTGCGCGCACTGTCCCGCAGCTATATTGGTGCCATC
GGCGGTTCTGACAACCTGACCGGCATTGATGCCTGTATCACCCGTTTACGCCTGACCGTTAAAGACTCTTCTAAAGTGCT
GGATGCCAGCTGTAAACGCCTGGGTGCATCAGGCGTGGTGAAACTGAACAAGCAAACCGTTCAGGTTATCGTGGGTTCTA
AAGCCGAAGCCATTGCTGACGGCATGCGTGCGGTGATTGCTGCCGGCCCGGTGCCTGCGGCAGAAGTGGCACCGGCTCCA
GGCGTGGCTGCGGTTAAATCGCAGGCAGTGCCTAATACGCCGAAGGTGGCCTTCGAGTCGCTGGTGGCCCCGGTGACTGG
TGAAGTCGTGGCCCTGGATCAGGTGCCTGACGAAGCCTTTGCCAGCAAAGCGGTAGGCGACGGCCTGGCGATTCGCCCGA
CCGACAAAACCGTAGTAGCACCGGCAGATGGCACCATCGTGAAAATCTTCAACACCAACCACGCTTTCTGTCTGGAAACC
GACAAGGGCGCGGAGATTGTGGTGCACATGGGGATCGACACCGTGGCATTGAACGGTCAGGGCTTCAAACGTCTGGTTGA
AGAGGGGGCTGAGGTGAAAGCCGGCCAGCCAATCCTTGAATTGGATCTGGAGTTCCTGAACGCCAATGCCCGCTCGATGA
TAAGCCCGGTAGTGGTCAGCAACTCTGACGATTACGCTGGCCTGACGGCGCTGGCGACCGGTTCTGTAGTAGCCGGCCAG
ACCAAACTGTTCGAGATCCAGAAATAA

Upstream 100 bases:

>100_bases
CTCCACGTGTTGTCGTTCGTCCAGAGTTAATCGCAGTGTGTGATGGCGCTTGCGTAAAATATGATAAAGCTCATTAAAGG
GCATTAGGGGGAATATTAAG

Downstream 100 bases:

>100_bases
ACCGTTTTACCTGAGTAGTACCCGGGGTGTTTAACGAGTCATGACGGAACGCACCCTACCTTTGGCGGGAAGAGAGCAAT
CTCTTCCCGCTTTTTTATGG

Product: PTS system N-acetyl glucosamine specific transporter subunits IIABC

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 648; Mature: 648

Protein sequence:

>648_residues
MNILGFFQRLGRSLQLPIAVLPVAALLLRFGQPDLLNISFIAQAGGSIFDNLALIFAIGVAATWSKDNAGSAALAGAVGY
FILTKAMVTINPAINMGVLAGIITGLVGGAVYNRWSGIKLPEFLSFFGGKRFVPIATGFFCLILAAIFGYIWPPVQNAIH
AGGEWIVSMGAIGSGIFGFVNRLLIPTGLHQVLNTIAWFQIGEFTNAAGTIFHGDINRFYAGDGTAGMFMSGFFPIMMFG
LPGAALAMYFAAPKERRPMVGGMLLSVALTAFLTGVTEPLEFLFMFLAPLLYLVHAILTGISLFVATLLGIHAGFSFSAG
AIDYVLMYNLPAASKNVWMLVLMGLVAFAVYFVLFSVIIRAFNLKTPGREDASDDVVAPEANSNTDEGLRALSRSYIGAI
GGSDNLTGIDACITRLRLTVKDSSKVLDASCKRLGASGVVKLNKQTVQVIVGSKAEAIADGMRAVIAAGPVPAAEVAPAP
GVAAVKSQAVPNTPKVAFESLVAPVTGEVVALDQVPDEAFASKAVGDGLAIRPTDKTVVAPADGTIVKIFNTNHAFCLET
DKGAEIVVHMGIDTVALNGQGFKRLVEEGAEVKAGQPILELDLEFLNANARSMISPVVVSNSDDYAGLTALATGSVVAGQ
TKLFEIQK

Sequences:

>Translated_648_residues
MNILGFFQRLGRSLQLPIAVLPVAALLLRFGQPDLLNISFIAQAGGSIFDNLALIFAIGVAATWSKDNAGSAALAGAVGY
FILTKAMVTINPAINMGVLAGIITGLVGGAVYNRWSGIKLPEFLSFFGGKRFVPIATGFFCLILAAIFGYIWPPVQNAIH
AGGEWIVSMGAIGSGIFGFVNRLLIPTGLHQVLNTIAWFQIGEFTNAAGTIFHGDINRFYAGDGTAGMFMSGFFPIMMFG
LPGAALAMYFAAPKERRPMVGGMLLSVALTAFLTGVTEPLEFLFMFLAPLLYLVHAILTGISLFVATLLGIHAGFSFSAG
AIDYVLMYNLPAASKNVWMLVLMGLVAFAVYFVLFSVIIRAFNLKTPGREDASDDVVAPEANSNTDEGLRALSRSYIGAI
GGSDNLTGIDACITRLRLTVKDSSKVLDASCKRLGASGVVKLNKQTVQVIVGSKAEAIADGMRAVIAAGPVPAAEVAPAP
GVAAVKSQAVPNTPKVAFESLVAPVTGEVVALDQVPDEAFASKAVGDGLAIRPTDKTVVAPADGTIVKIFNTNHAFCLET
DKGAEIVVHMGIDTVALNGQGFKRLVEEGAEVKAGQPILELDLEFLNANARSMISPVVVSNSDDYAGLTALATGSVVAGQ
TKLFEIQK
>Mature_648_residues
MNILGFFQRLGRSLQLPIAVLPVAALLLRFGQPDLLNISFIAQAGGSIFDNLALIFAIGVAATWSKDNAGSAALAGAVGY
FILTKAMVTINPAINMGVLAGIITGLVGGAVYNRWSGIKLPEFLSFFGGKRFVPIATGFFCLILAAIFGYIWPPVQNAIH
AGGEWIVSMGAIGSGIFGFVNRLLIPTGLHQVLNTIAWFQIGEFTNAAGTIFHGDINRFYAGDGTAGMFMSGFFPIMMFG
LPGAALAMYFAAPKERRPMVGGMLLSVALTAFLTGVTEPLEFLFMFLAPLLYLVHAILTGISLFVATLLGIHAGFSFSAG
AIDYVLMYNLPAASKNVWMLVLMGLVAFAVYFVLFSVIIRAFNLKTPGREDASDDVVAPEANSNTDEGLRALSRSYIGAI
GGSDNLTGIDACITRLRLTVKDSSKVLDASCKRLGASGVVKLNKQTVQVIVGSKAEAIADGMRAVIAAGPVPAAEVAPAP
GVAAVKSQAVPNTPKVAFESLVAPVTGEVVALDQVPDEAFASKAVGDGLAIRPTDKTVVAPADGTIVKIFNTNHAFCLET
DKGAEIVVHMGIDTVALNGQGFKRLVEEGAEVKAGQPILELDLEFLNANARSMISPVVVSNSDDYAGLTALATGSVVAGQ
TKLFEIQK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=650, Percent_Identity=82, Blast_Score=1041, Evalue=0.0,
Organism=Escherichia coli, GI1787343, Length=485, Percent_Identity=43.298969072165, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1787908, Length=511, Percent_Identity=33.4637964774951, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI1788757, Length=148, Percent_Identity=52.7027027027027, Blast_Score=162, Evalue=7e-41,
Organism=Escherichia coli, GI1790159, Length=170, Percent_Identity=35.8823529411765, Blast_Score=109, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 67990; Mature: 67990

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1 ; PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILGFFQRLGRSLQLPIAVLPVAALLLRFGQPDLLNISFIAQAGGSIFDNLALIFAIGV
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHH
AATWSKDNAGSAALAGAVGYFILTKAMVTINPAINMGVLAGIITGLVGGAVYNRWSGIKL
HEECCCCCCCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
PEFLSFFGGKRFVPIATGFFCLILAAIFGYIWPPVQNAIHAGGEWIVSMGAIGSGIFGFV
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHEEECCCCCCHHHHHH
NRLLIPTGLHQVLNTIAWFQIGEFTNAAGTIFHGDINRFYAGDGTAGMFMSGFFPIMMFG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHHC
LPGAALAMYFAAPKERRPMVGGMLLSVALTAFLTGVTEPLEFLFMFLAPLLYLVHAILTG
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
ISLFVATLLGIHAGFSFSAGAIDYVLMYNLPAASKNVWMLVLMGLVAFAVYFVLFSVIIR
HHHHHHHHHHHHCCCCCCCCHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AFNLKTPGREDASDDVVAPEANSNTDEGLRALSRSYIGAIGGSDNLTGIDACITRLRLTV
HHCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEEE
KDSSKVLDASCKRLGASGVVKLNKQTVQVIVGSKAEAIADGMRAVIAAGPVPAAEVAPAP
CCCHHHHHHHHHHCCCCCEEEECCEEEEEEECCCHHHHHHHHHHHHHCCCCCHHHCCCCC
GVAAVKSQAVPNTPKVAFESLVAPVTGEVVALDQVPDEAFASKAVGDGLAIRPTDKTVVA
CHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHCCCCEEECCCCCEEEE
PADGTIVKIFNTNHAFCLETDKGAEIVVHMGIDTVALNGQGFKRLVEEGAEVKAGQPILE
CCCCCEEEEEECCCEEEEECCCCCEEEEEECCCEEEECCHHHHHHHHCCCCCCCCCCHHH
LDLEFLNANARSMISPVVVSNSDDYAGLTALATGSVVAGQTKLFEIQK
HHHHHHCCCHHHHHCCEEEECCCCCCCHHHHCCCCEEECCCEEEEECC
>Mature Secondary Structure
MNILGFFQRLGRSLQLPIAVLPVAALLLRFGQPDLLNISFIAQAGGSIFDNLALIFAIGV
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHH
AATWSKDNAGSAALAGAVGYFILTKAMVTINPAINMGVLAGIITGLVGGAVYNRWSGIKL
HEECCCCCCCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
PEFLSFFGGKRFVPIATGFFCLILAAIFGYIWPPVQNAIHAGGEWIVSMGAIGSGIFGFV
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHEEECCCCCCHHHHHH
NRLLIPTGLHQVLNTIAWFQIGEFTNAAGTIFHGDINRFYAGDGTAGMFMSGFFPIMMFG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHHC
LPGAALAMYFAAPKERRPMVGGMLLSVALTAFLTGVTEPLEFLFMFLAPLLYLVHAILTG
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
ISLFVATLLGIHAGFSFSAGAIDYVLMYNLPAASKNVWMLVLMGLVAFAVYFVLFSVIIR
HHHHHHHHHHHHCCCCCCCCHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AFNLKTPGREDASDDVVAPEANSNTDEGLRALSRSYIGAIGGSDNLTGIDACITRLRLTV
HHCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEEE
KDSSKVLDASCKRLGASGVVKLNKQTVQVIVGSKAEAIADGMRAVIAAGPVPAAEVAPAP
CCCHHHHHHHHHHCCCCCEEEECCEEEEEEECCCHHHHHHHHHHHHHCCCCCHHHCCCCC
GVAAVKSQAVPNTPKVAFESLVAPVTGEVVALDQVPDEAFASKAVGDGLAIRPTDKTVVA
CHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHCCCCEEECCCCCEEEE
PADGTIVKIFNTNHAFCLETDKGAEIVVHMGIDTVALNGQGFKRLVEEGAEVKAGQPILE
CCCCCEEEEEECCCEEEEECCCCCEEEEEECCCEEEECCHHHHHHHHCCCCCCCCCCHHH
LDLEFLNANARSMISPVVVSNSDDYAGLTALATGSVVAGQTKLFEIQK
HHHHHHCCCHHHHHCCEEEECCCCCCCHHHHCCCCEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3284790; 3056518; 8905232; 9278503 [H]