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The map label for this gene is potE [H]

Identifier: 157368755

GI number: 157368755

Start: 560448

End: 561770

Strand: Reverse

Name: potE [H]

Synonym: Spro_0508

Alternate gene names: 157368755

Gene position: 561770-560448 (Counterclockwise)

Preceding gene: 157368756

Following gene: 157368754

Centisome position: 10.31

GC content: 55.71

Gene sequence:

>1323_bases
ATGATCATGAGTAAGTCCAATAATAAAATGGGGGTCGTACAGCTAACGATCCTTACTGCGGTAAACATGATGGGCTCCGG
CATAATTATGCTGCCCACCAAGCTGGCCGAAGTGGGTACTATTTCTATCGTTTCCTGGCTGGTTACCGCCGTGGGCTCGA
TGGCGCTAGCCTACGCCTTCGCCAAATGCGGCATGTTCAGCCGCAAATCCGGCGGCATGGGCGGGTATGCTGAATACGCT
TTCGGTAAATCGGGCAATTTCATGGCGAACTACACCTATGGGGTGTCGTTGCTGATCGCCAATATCGCCATCGCCATTTC
CGCCGTTGGTTACGGCACCGAGCTGTTTGGTGCCAACCTGTCGCCGCTGGGGATCTGTATCGCCACCATTGGCGTACTAT
GGCTGGCTACCGTCGCCAACTTCGGCGGGGCACGCCTGACCGGGAAAATCAGCGGCATCACCGTTTGGGGCGTGATTATC
CCGGTGGTGGGGATCTCGATCATCGGCTGGCACTGGTTCAGCGGCAGCGCCTATATTGCCGCCTGGAACCCGCACTCGGT
ACCGACCTTTGAAGCCATTGGCGCTTCTATCTCCATGACGCTGTGGGCATTTCTGGGCCTGGAGTCCGCCTGCGCCAATA
CCGACGTGGTGGAAAACCCGGAGCGTAACGTGCCGATCGCCGTACTCGGCGGTACCCTCAGCGCGGCAGTCATCTATATC
GTTTCAACCAACGTCATCGCCGGTATCGTGCCGAATATGGACCTGGCCAACTCAACCGCGCCATTTGGGCTGGCGTTTTC
CCACATGTTCAACCCAACGGTCGGCAAGATCATTATGGCCCTGATGATCATGTCCTGCGTCGGCTCACTGCTGGGCTGGC
AGTTCACCATCGCCCAGGTGTTCAAATCCTCTGCCGACAGCGGCTTCTTCCCGAAAATTTTCTCCAAACTCAGCAAGGCC
GACGCACCGGTCAAAGGCATGCTGACCATAGTCATGATCCAGAGCGTGCTGTCTTTGATGACCATCAGCCCGTCGCTGAA
TAAACAGTTCAATGTGCTGGTGAATCTGGCGGTGGTGACCAATATCATCCCTTACATTCTGTCGATGGCGGCGCTGATCA
TTATTCAGAAAGTGGCGAAAGTACCGGAGAAAAAAGCGCGAATTGCCAACATCATTGCCGGCATTAGCGCACTGTACAGT
TTCTATGCCCTCTACAGTTCCGGTGAGGAAGCGATGATGTGGGGCGCCATCGCCACCTTCCTCGGCTGGACGCTGTACGG
TATGGTTTCACCGCGTTTCGAAATGGCGGGGAAAAAAGGCTAA

Upstream 100 bases:

>100_bases
AGATGGCTGGAACCGTGCTTACGGTTACGTAATGAAAAATTAATCACCTTACAAGGCGGTGAATTTTATTCACCGCCCGG
ATCGTATTATAAAAGAGAGA

Downstream 100 bases:

>100_bases
CGGTGATAAGCCATAGGCGGCCGGGGATGGGATTACGACGGGGAACATGCCATCATATTCCATCCCTGGCCACCAACGTT
GCTGCTTATTCATGACGGAA

Product: putrescine transporter

Products: Proton [Cytoplasm]; putrescine [Cytoplasm] [C]

Alternate protein names: Putrescine transport protein [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MIMSKSNNKMGVVQLTILTAVNMMGSGIIMLPTKLAEVGTISIVSWLVTAVGSMALAYAFAKCGMFSRKSGGMGGYAEYA
FGKSGNFMANYTYGVSLLIANIAIAISAVGYGTELFGANLSPLGICIATIGVLWLATVANFGGARLTGKISGITVWGVII
PVVGISIIGWHWFSGSAYIAAWNPHSVPTFEAIGASISMTLWAFLGLESACANTDVVENPERNVPIAVLGGTLSAAVIYI
VSTNVIAGIVPNMDLANSTAPFGLAFSHMFNPTVGKIIMALMIMSCVGSLLGWQFTIAQVFKSSADSGFFPKIFSKLSKA
DAPVKGMLTIVMIQSVLSLMTISPSLNKQFNVLVNLAVVTNIIPYILSMAALIIIQKVAKVPEKKARIANIIAGISALYS
FYALYSSGEEAMMWGAIATFLGWTLYGMVSPRFEMAGKKG

Sequences:

>Translated_440_residues
MIMSKSNNKMGVVQLTILTAVNMMGSGIIMLPTKLAEVGTISIVSWLVTAVGSMALAYAFAKCGMFSRKSGGMGGYAEYA
FGKSGNFMANYTYGVSLLIANIAIAISAVGYGTELFGANLSPLGICIATIGVLWLATVANFGGARLTGKISGITVWGVII
PVVGISIIGWHWFSGSAYIAAWNPHSVPTFEAIGASISMTLWAFLGLESACANTDVVENPERNVPIAVLGGTLSAAVIYI
VSTNVIAGIVPNMDLANSTAPFGLAFSHMFNPTVGKIIMALMIMSCVGSLLGWQFTIAQVFKSSADSGFFPKIFSKLSKA
DAPVKGMLTIVMIQSVLSLMTISPSLNKQFNVLVNLAVVTNIIPYILSMAALIIIQKVAKVPEKKARIANIIAGISALYS
FYALYSSGEEAMMWGAIATFLGWTLYGMVSPRFEMAGKKG
>Mature_440_residues
MIMSKSNNKMGVVQLTILTAVNMMGSGIIMLPTKLAEVGTISIVSWLVTAVGSMALAYAFAKCGMFSRKSGGMGGYAEYA
FGKSGNFMANYTYGVSLLIANIAIAISAVGYGTELFGANLSPLGICIATIGVLWLATVANFGGARLTGKISGITVWGVII
PVVGISIIGWHWFSGSAYIAAWNPHSVPTFEAIGASISMTLWAFLGLESACANTDVVENPERNVPIAVLGGTLSAAVIYI
VSTNVIAGIVPNMDLANSTAPFGLAFSHMFNPTVGKIIMALMIMSCVGSLLGWQFTIAQVFKSSADSGFFPKIFSKLSKA
DAPVKGMLTIVMIQSVLSLMTISPSLNKQFNVLVNLAVVTNIIPYILSMAALIIIQKVAKVPEKKARIANIIAGISALYS
FYALYSSGEEAMMWGAIATFLGWTLYGMVSPRFEMAGKKG

Specific function: Probable putrescine-ornithine antiporter [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786908, Length=439, Percent_Identity=82.4601366742597, Blast_Score=734, Evalue=0.0,
Organism=Escherichia coli, GI2367353, Length=407, Percent_Identity=36.3636363636364, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI1790575, Length=382, Percent_Identity=34.5549738219895, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1787890, Length=424, Percent_Identity=27.3584905660377, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1787768, Length=289, Percent_Identity=22.8373702422145, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17540018, Length=331, Percent_Identity=27.190332326284, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004754 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 46386; Mature: 46386

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMSKSNNKMGVVQLTILTAVNMMGSGIIMLPTKLAEVGTISIVSWLVTAVGSMALAYAF
CCCCCCCCCCEEEEEHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANIAIAISAVGYGTELFGANL
HHCCCCCCCCCCCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
SPLGICIATIGVLWLATVANFGGARLTGKISGITVWGVIIPVVGISIIGWHWFSGSAYIA
CHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHEEEEECCCEEEE
AWNPHSVPTFEAIGASISMTLWAFLGLESACANTDVVENPERNVPIAVLGGTLSAAVIYI
EECCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHE
VSTNVIAGIVPNMDLANSTAPFGLAFSHMFNPTVGKIIMALMIMSCVGSLLGWQFTIAQV
EEHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKSSADSGFFPKIFSKLSKADAPVKGMLTIVMIQSVLSLMTISPSLNKQFNVLVNLAVVT
HHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
NIIPYILSMAALIIIQKVAKVPEKKARIANIIAGISALYSFYALYSSGEEAMMWGAIATF
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LGWTLYGMVSPRFEMAGKKG
HHHHHHHHCCCHHHCCCCCC
>Mature Secondary Structure
MIMSKSNNKMGVVQLTILTAVNMMGSGIIMLPTKLAEVGTISIVSWLVTAVGSMALAYAF
CCCCCCCCCCEEEEEHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANIAIAISAVGYGTELFGANL
HHCCCCCCCCCCCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
SPLGICIATIGVLWLATVANFGGARLTGKISGITVWGVIIPVVGISIIGWHWFSGSAYIA
CHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHEEEEECCCEEEE
AWNPHSVPTFEAIGASISMTLWAFLGLESACANTDVVENPERNVPIAVLGGTLSAAVIYI
EECCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHE
VSTNVIAGIVPNMDLANSTAPFGLAFSHMFNPTVGKIIMALMIMSCVGSLLGWQFTIAQV
EEHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKSSADSGFFPKIFSKLSKADAPVKGMLTIVMIQSVLSLMTISPSLNKQFNVLVNLAVVT
HHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
NIIPYILSMAALIIIQKVAKVPEKKARIANIIAGISALYSFYALYSSGEEAMMWGAIATF
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LGWTLYGMVSPRFEMAGKKG
HHHHHHHHCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; putrescine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + putrescine [Periplasm] = Proton [Cytoplasm] + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]