Click here to switch to the map view.

The map label for this gene is pmrA [H]

Identifier: 157368725

GI number: 157368725

Start: 528084

End: 528746

Strand: Reverse

Name: pmrA [H]

Synonym: Spro_0478

Alternate gene names: 157368725

Gene position: 528746-528084 (Counterclockwise)

Preceding gene: 157368726

Following gene: 157368724

Centisome position: 9.7

GC content: 58.37

Gene sequence:

>663_bases
ATGAAACTGCTGATTGTTGAAGACGATGAGCTGCTGCAGCAAGGCCTGGCCCTGGCATTAGCTGGCGAGGGCTATGCCTG
CGACTGCGCGGCTACGGCCGCGGAGGCCAATGCTCTGCTCCTCACCAGTCAGTACAGCATGATTATCCTCGATCTGGGCC
TGCCTGACATGGACGGCGCCAGCCTGCTTCGGCAGTGGCGCCGTCAGCAAGTCGAGCTGCCGGTGCTGATCCTGACCGCT
CGCGATGCGCTTGAAGACCGTGTTGATGGGCTGGACGCCGGCGCCGACGATTATCTGGTGAAACCCTTCGCGTTGGTTGA
ACTGCAGGCGCGCGTGCGGGCGCTGATCCGTCGCTATCAGGGTCACAGTGACAACCTCATGCAGCTCGATGACCTCCAAC
TCAATCTCTCCAGTCAGCAGGTTTATGTTCAGCAACAGCCGGTGGAAGTCACCCCGAAAGAGTTCGCCATCCTGTCACGT
CTGATGATGCGCGCCGGGCAAACGGTCAATCGTGAACTGCTGCAGCAGGATCTCTATACCTGGCAGGACGATTTGGGCTC
CAACACGCTGGAAGTACATATCCATAATCTGCGCCGCAAATTGGGTAAAGATCGCATCCGCACCGTTCGCGGTATCGGCT
ATCGTCTGGAATCCTCATCATGA

Upstream 100 bases:

>100_bases
ATGCCGTGCCGCCGGAAGATCAAAAACAGCGCCGCGCTCCACTGGTGCGTTTCGAAAGCCGTCTGTACCGGGATATTTAT
CAGAATAACTGAGGGTTATG

Downstream 100 bases:

>100_bases
TCAGCATGCGCCGTCGTCTGTTGTTGATGCTGGCGCTGATCCTTTTGGTCACCCAGCTTATCAGTGCCTTTTGGTTGTGG
CATGAAAGCCAAGAGCAAAT

Product: DNA-binding transcriptional regulator BasR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MKLLIVEDDELLQQGLALALAGEGYACDCAATAAEANALLLTSQYSMIILDLGLPDMDGASLLRQWRRQQVELPVLILTA
RDALEDRVDGLDAGADDYLVKPFALVELQARVRALIRRYQGHSDNLMQLDDLQLNLSSQQVYVQQQPVEVTPKEFAILSR
LMMRAGQTVNRELLQQDLYTWQDDLGSNTLEVHIHNLRRKLGKDRIRTVRGIGYRLESSS

Sequences:

>Translated_220_residues
MKLLIVEDDELLQQGLALALAGEGYACDCAATAAEANALLLTSQYSMIILDLGLPDMDGASLLRQWRRQQVELPVLILTA
RDALEDRVDGLDAGADDYLVKPFALVELQARVRALIRRYQGHSDNLMQLDDLQLNLSSQQVYVQQQPVEVTPKEFAILSR
LMMRAGQTVNRELLQQDLYTWQDDLGSNTLEVHIHNLRRKLGKDRIRTVRGIGYRLESSS
>Mature_220_residues
MKLLIVEDDELLQQGLALALAGEGYACDCAATAAEANALLLTSQYSMIILDLGLPDMDGASLLRQWRRQQVELPVLILTA
RDALEDRVDGLDAGADDYLVKPFALVELQARVRALIRRYQGHSDNLMQLDDLQLNLSSQQVYVQQQPVEVTPKEFAILSR
LMMRAGQTVNRELLQQDLYTWQDDLGSNTLEVHIHNLRRKLGKDRIRTVRGIGYRLESSS

Specific function: Member of the two-component regulatory system pmrB/pmrA involved in regulation of virulence. Unphosphorylated pmrA represses extracellular enzyme genes. Phosphorylation of pmrA by pmrB relieves such repression, which leads to activation of extracellular e

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790552, Length=216, Percent_Identity=56.4814814814815, Blast_Score=248, Evalue=2e-67,
Organism=Escherichia coli, GI1789402, Length=216, Percent_Identity=46.2962962962963, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1787375, Length=221, Percent_Identity=38.0090497737557, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1786784, Length=222, Percent_Identity=38.7387387387387, Blast_Score=146, Evalue=9e-37,
Organism=Escherichia coli, GI87082012, Length=220, Percent_Identity=37.2727272727273, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1786911, Length=225, Percent_Identity=37.7777777777778, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1786599, Length=224, Percent_Identity=33.0357142857143, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1789809, Length=225, Percent_Identity=31.5555555555556, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1790860, Length=219, Percent_Identity=34.2465753424658, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1788394, Length=227, Percent_Identity=33.920704845815, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI2367329, Length=232, Percent_Identity=37.0689655172414, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1787229, Length=222, Percent_Identity=31.5315315315315, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790863, Length=228, Percent_Identity=27.1929824561404, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1790299, Length=131, Percent_Identity=35.1145038167939, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 24775; Mature: 24775

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLIVEDDELLQQGLALALAGEGYACDCAATAAEANALLLTSQYSMIILDLGLPDMDGA
CEEEEECCHHHHHCCCEEEECCCCCEECHHHHHHCCCEEEEECCCEEEEEECCCCCCCHH
SLLRQWRRQQVELPVLILTARDALEDRVDGLDAGADDYLVKPFALVELQARVRALIRRYQ
HHHHHHHHHCCCCCEEEEECHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHC
GHSDNLMQLDDLQLNLSSQQVYVQQQPVEVTPKEFAILSRLMMRAGQTVNRELLQQDLYT
CCCCCCEEEECEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
WQDDLGSNTLEVHIHNLRRKLGKDRIRTVRGIGYRLESSS
HHHHCCCCEEEEHHHHHHHHHCHHHHHHHHHCCEEECCCC
>Mature Secondary Structure
MKLLIVEDDELLQQGLALALAGEGYACDCAATAAEANALLLTSQYSMIILDLGLPDMDGA
CEEEEECCHHHHHCCCEEEECCCCCEECHHHHHHCCCEEEEECCCEEEEEECCCCCCCHH
SLLRQWRRQQVELPVLILTARDALEDRVDGLDAGADDYLVKPFALVELQARVRALIRRYQ
HHHHHHHHHCCCCCEEEEECHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHC
GHSDNLMQLDDLQLNLSSQQVYVQQQPVEVTPKEFAILSRLMMRAGQTVNRELLQQDLYT
CCCCCCEEEECEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
WQDDLGSNTLEVHIHNLRRKLGKDRIRTVRGIGYRLESSS
HHHHCCCCEEEEHHHHHHHHHCHHHHHHHHHCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14617142 [H]