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The map label for this gene is pmrB [H]

Identifier: 157368724

GI number: 157368724

Start: 527026

End: 528087

Strand: Reverse

Name: pmrB [H]

Synonym: Spro_0477

Alternate gene names: 157368724

Gene position: 528087-527026 (Counterclockwise)

Preceding gene: 157368725

Following gene: 157368719

Centisome position: 9.69

GC content: 55.46

Gene sequence:

>1062_bases
ATGATCAGCATGCGCCGTCGTCTGTTGTTGATGCTGGCGCTGATCCTTTTGGTCACCCAGCTTATCAGTGCCTTTTGGTT
GTGGCATGAAAGCCAAGAGCAAATCAGCTTCCTGGTGGATGAAACCCTGTCGGCCAAGGTTCGCACCGAGCGTGTCGATA
CGGAAATCGCCGAAGCAATAGCCTCACTGCTCGCCCCTTCACTGATCATGATGGTCGTGACTTTGCTCGCCTCATTTTGG
GCTATCAGCTGGATTATCCGCCCGCTCAATCAGTTGCAACAACGGCTGGAAAAGCGTTCTGCCGATAACCTGACGCCGCT
GCCGCTTAACAGCGACAGCCTCGAAATAATGTCGGTCACCACTACGCTCAACCAATTGTTCTCGCGGCTGGATAACACCA
TCCAGCAGGAACGGCTGTTTACTGCCGACGCCGCCCATGAGCTACGCACGCCTCTGGCGGGCATCCGCCTGCATCTGGAG
TTAATGGAAAAACAGGGGGTCCCGCAAAGTGGGCCACTGATTAAGCGCATTGACCAACTGATGCACACCGTCGAACAATT
GCTGATGCTTGCACGCGCCGGGCAAGATTTTGCCAGTGGCCATTATCAACGCTTCGATTGGGTGAGCGATGTGATCCAAC
CGCTGCGGGAAGAGCTCGAGGAGTTGGCCACACAACGCGGGCAAAGTTTGCAATGGCAGCTTCCGACCACCGCCCCGGTG
CATGGTGACCCGGTACTGTTACGTCTGCTGCTGCGTAACCTGGTGGAGAATGCCCATCGTTATAGCCCTGAGGGTAGCAG
GATCCAGGTACAACTTTCTGCGCAGGAACGCGGTTACCGGCTACAGGTGATCGATGAAGGTCCGGGCGTCAAAGAGAAAA
TAACGGGTGAACTCACGCAGGCCTTCCGCCGCATGGATCAACGCTATGGCGGCAGTGGGTTGGGATTGAATATCGTAACT
CGTATTGTGCAGCTGCATCAGGGGCGTTTAACACTGGAAAACCGCCAGGACACCAGCGGGCTCAATGCACAATGCTGGCT
CCCGGAAAACGTCCTGAAATAA

Upstream 100 bases:

>100_bases
CCAACACGCTGGAAGTACATATCCATAATCTGCGCCGCAAATTGGGTAAAGATCGCATCCGCACCGTTCGCGGTATCGGC
TATCGTCTGGAATCCTCATC

Downstream 100 bases:

>100_bases
AAAAAGGGCCAGTGTTAACTGAGCCCCTCTCTTCACAAGATTACTTCTGGTAAATGATTTCGACGCCTTCGTCGTCCTCT
TCATCCCAGTCGTCATCCCA

Product: sensor protein BasS/PmrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MISMRRRLLLMLALILLVTQLISAFWLWHESQEQISFLVDETLSAKVRTERVDTEIAEAIASLLAPSLIMMVVTLLASFW
AISWIIRPLNQLQQRLEKRSADNLTPLPLNSDSLEIMSVTTTLNQLFSRLDNTIQQERLFTADAAHELRTPLAGIRLHLE
LMEKQGVPQSGPLIKRIDQLMHTVEQLLMLARAGQDFASGHYQRFDWVSDVIQPLREELEELATQRGQSLQWQLPTTAPV
HGDPVLLRLLLRNLVENAHRYSPEGSRIQVQLSAQERGYRLQVIDEGPGVKEKITGELTQAFRRMDQRYGGSGLGLNIVT
RIVQLHQGRLTLENRQDTSGLNAQCWLPENVLK

Sequences:

>Translated_353_residues
MISMRRRLLLMLALILLVTQLISAFWLWHESQEQISFLVDETLSAKVRTERVDTEIAEAIASLLAPSLIMMVVTLLASFW
AISWIIRPLNQLQQRLEKRSADNLTPLPLNSDSLEIMSVTTTLNQLFSRLDNTIQQERLFTADAAHELRTPLAGIRLHLE
LMEKQGVPQSGPLIKRIDQLMHTVEQLLMLARAGQDFASGHYQRFDWVSDVIQPLREELEELATQRGQSLQWQLPTTAPV
HGDPVLLRLLLRNLVENAHRYSPEGSRIQVQLSAQERGYRLQVIDEGPGVKEKITGELTQAFRRMDQRYGGSGLGLNIVT
RIVQLHQGRLTLENRQDTSGLNAQCWLPENVLK
>Mature_353_residues
MISMRRRLLLMLALILLVTQLISAFWLWHESQEQISFLVDETLSAKVRTERVDTEIAEAIASLLAPSLIMMVVTLLASFW
AISWIIRPLNQLQQRLEKRSADNLTPLPLNSDSLEIMSVTTTLNQLFSRLDNTIQQERLFTADAAHELRTPLAGIRLHLE
LMEKQGVPQSGPLIKRIDQLMHTVEQLLMLARAGQDFASGHYQRFDWVSDVIQPLREELEELATQRGQSLQWQLPTTAPV
HGDPVLLRLLLRNLVENAHRYSPEGSRIQVQLSAQERGYRLQVIDEGPGVKEKITGELTQAFRRMDQRYGGSGLGLNIVT
RIVQLHQGRLTLENRQDTSGLNAQCWLPENVLK

Specific function: Member of the two-component regulatory system pmrB/pmrA involved in regulation of virulence. Functions probably as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to pmrA in response to external

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790551, Length=352, Percent_Identity=52.5568181818182, Blast_Score=350, Evalue=5e-98,
Organism=Escherichia coli, GI1789403, Length=302, Percent_Identity=32.7814569536424, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788393, Length=259, Percent_Identity=32.8185328185328, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1790346, Length=293, Percent_Identity=27.6450511945392, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1789808, Length=304, Percent_Identity=28.2894736842105, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786600, Length=265, Percent_Identity=30.9433962264151, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786783, Length=294, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788279, Length=289, Percent_Identity=25.9515570934256, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790861, Length=287, Percent_Identity=25.0871080139373, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789149, Length=221, Percent_Identity=28.5067873303167, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788713, Length=214, Percent_Identity=28.5046728971963, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787894, Length=223, Percent_Identity=26.457399103139, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40212; Mature: 40212

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISMRRRLLLMLALILLVTQLISAFWLWHESQEQISFLVDETLSAKVRTERVDTEIAEAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASLLAPSLIMMVVTLLASFWAISWIIRPLNQLQQRLEKRSADNLTPLPLNSDSLEIMSVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
TTLNQLFSRLDNTIQQERLFTADAAHELRTPLAGIRLHLELMEKQGVPQSGPLIKRIDQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
MHTVEQLLMLARAGQDFASGHYQRFDWVSDVIQPLREELEELATQRGQSLQWQLPTTAPV
HHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
HGDPVLLRLLLRNLVENAHRYSPEGSRIQVQLSAQERGYRLQVIDEGPGVKEKITGELTQ
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHH
AFRRMDQRYGGSGLGLNIVTRIVQLHQGRLTLENRQDTSGLNAQCWLPENVLK
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCEEECCHHHCC
>Mature Secondary Structure
MISMRRRLLLMLALILLVTQLISAFWLWHESQEQISFLVDETLSAKVRTERVDTEIAEAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASLLAPSLIMMVVTLLASFWAISWIIRPLNQLQQRLEKRSADNLTPLPLNSDSLEIMSVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
TTLNQLFSRLDNTIQQERLFTADAAHELRTPLAGIRLHLELMEKQGVPQSGPLIKRIDQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
MHTVEQLLMLARAGQDFASGHYQRFDWVSDVIQPLREELEELATQRGQSLQWQLPTTAPV
HHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
HGDPVLLRLLLRNLVENAHRYSPEGSRIQVQLSAQERGYRLQVIDEGPGVKEKITGELTQ
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHH
AFRRMDQRYGGSGLGLNIVTRIVQLHQGRLTLENRQDTSGLNAQCWLPENVLK
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 14617142 [H]