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The map label for this gene is dacB [H]

Identifier: 157368726

GI number: 157368726

Start: 528755

End: 530188

Strand: Reverse

Name: dacB [H]

Synonym: Spro_0479

Alternate gene names: 157368726

Gene position: 530188-528755 (Counterclockwise)

Preceding gene: 157368728

Following gene: 157368725

Centisome position: 9.73

GC content: 57.74

Gene sequence:

>1434_bases
ATGCGTTTTTCACGAATTGTCAGTGCATTGGCTTGCGCATTTGTTCTAAATGCGAATGCGGCCCCGGTTGAAGATTACAC
ACAATATTTGCCTGATGGGGCCAACCTTGCCCTGGTAGTTCAGAAGATCGGCGCAGACGCACCGACCATCGATTATCACT
CTCAGCAAATGGCGTTGCCGGCCAGTACCCAGAAAGTGCTGACCGCGCTGGCTGCATTATTGCAGCTCGGCCCCGACTAT
CGCTTCACCACCACGCTGGAAAGCCAGGGTGATATCAGTGACGGCGTGCTGCGCGGCAACCTGATTGCCCGCTTCAGCGG
CGATCCGACCTTTAAGCGCCAAAGCCTGCGTAACATGGTGGCGATCCTGAAAAAGCAGGGTGTCCGCCAGATCACCGGCG
ATGTGCTGGTCGATACTTCGGTATTCGCCAGCCATGACAAAGCCCCCGGCTGGCCGTGGAACGACCTGACCCAGTGCTTC
AGCGCACCGCCGGCAGCGGCGATAGTCGATCGCAACTGTTTCTCTGTGTCGTTGTACAGCGCGCCGAATCCGGGCGATAT
GGCCTTTATTCGCGTGGCCTCGTATTACCCGGTCAATATGTTCAGCCAGGTACGTACGCTGGCGAAAGGCTCCGCAGATG
CCCAATATTGTGAGCTGGACGTGGTGCCGGGCGAGTTGAATCGCTTTACCCTGACCGGCTGTCTGACCCAACGCAGCGAT
CCGCTGCCGCTGGCCTTCGCCATTCAGGATGGTGCCAGCTATGCCGGGGCTATTTTGAAAGATGAGCTGACGCAGGCCGG
CATTCAGATCGACGGCCACCTGAAGCGTCAGACCCAACCTGGCCTGAAGGGAACGGTGATCGCACAGGCTCAATCGGCAC
CTCTGCACGAGTTGCTGAAGATCATGCTGAAGAAATCGGACAACATGATTGCCGATACCGTATTCCGCACTATCGGCCAT
GAACGTTTTGGCGTGCCGGGCACCTGGCGTGCCGGTGCCGATGCCGTGCGTCAGGTACTTCGTCAGAAAGCCGGTGTCGA
TTTGGGCAACAGCATCGTGGTGGACGGCTCAGGCCTGTCACGCCATAACCTGCTGGCCCCGGCAACCATGATGCAGGCGT
TGCAGTACATCGCGCAGCATGACAGTGAGCTGAACTTTATTTCAATGCTGCCGCTGTCTGGCTATGACGGCACCTTACGT
TACCGTGGCGGCCTGCATGAAGCCGGTGTCGACGGGAAGGTTTCCGCTAAAACCGGGGCATTGCAAGGCGTGTACAACCT
GGCCGGGTTTATCACCACCGCCAGCGGCCAGCGTTTTGCATTCGTACAGTATCTGTCGGGCTATGCCGTGCCGCCGGAAG
ATCAAAAACAGCGCCGCGCTCCACTGGTGCGTTTCGAAAGCCGTCTGTACCGGGATATTTATCAGAATAACTGA

Upstream 100 bases:

>100_bases
ATACGCATTTTAGCTTAGAGTTCCTTGAGGGTCATCGTTTACTTTTTCCCTGAATGCACCGTAGTATGACGGCACGTTAT
CCAGCTGTTATCCCGAGATT

Downstream 100 bases:

>100_bases
GGGTTATGATGAAACTGCTGATTGTTGAAGACGATGAGCTGCTGCAGCAAGGCCTGGCCCTGGCATTAGCTGGCGAGGGC
TATGCCTGCGACTGCGCGGC

Product: D-alanyl-D-alanine carboxypeptidase/endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MRFSRIVSALACAFVLNANAAPVEDYTQYLPDGANLALVVQKIGADAPTIDYHSQQMALPASTQKVLTALAALLQLGPDY
RFTTTLESQGDISDGVLRGNLIARFSGDPTFKRQSLRNMVAILKKQGVRQITGDVLVDTSVFASHDKAPGWPWNDLTQCF
SAPPAAAIVDRNCFSVSLYSAPNPGDMAFIRVASYYPVNMFSQVRTLAKGSADAQYCELDVVPGELNRFTLTGCLTQRSD
PLPLAFAIQDGASYAGAILKDELTQAGIQIDGHLKRQTQPGLKGTVIAQAQSAPLHELLKIMLKKSDNMIADTVFRTIGH
ERFGVPGTWRAGADAVRQVLRQKAGVDLGNSIVVDGSGLSRHNLLAPATMMQALQYIAQHDSELNFISMLPLSGYDGTLR
YRGGLHEAGVDGKVSAKTGALQGVYNLAGFITTASGQRFAFVQYLSGYAVPPEDQKQRRAPLVRFESRLYRDIYQNN

Sequences:

>Translated_477_residues
MRFSRIVSALACAFVLNANAAPVEDYTQYLPDGANLALVVQKIGADAPTIDYHSQQMALPASTQKVLTALAALLQLGPDY
RFTTTLESQGDISDGVLRGNLIARFSGDPTFKRQSLRNMVAILKKQGVRQITGDVLVDTSVFASHDKAPGWPWNDLTQCF
SAPPAAAIVDRNCFSVSLYSAPNPGDMAFIRVASYYPVNMFSQVRTLAKGSADAQYCELDVVPGELNRFTLTGCLTQRSD
PLPLAFAIQDGASYAGAILKDELTQAGIQIDGHLKRQTQPGLKGTVIAQAQSAPLHELLKIMLKKSDNMIADTVFRTIGH
ERFGVPGTWRAGADAVRQVLRQKAGVDLGNSIVVDGSGLSRHNLLAPATMMQALQYIAQHDSELNFISMLPLSGYDGTLR
YRGGLHEAGVDGKVSAKTGALQGVYNLAGFITTASGQRFAFVQYLSGYAVPPEDQKQRRAPLVRFESRLYRDIYQNN
>Mature_477_residues
MRFSRIVSALACAFVLNANAAPVEDYTQYLPDGANLALVVQKIGADAPTIDYHSQQMALPASTQKVLTALAALLQLGPDY
RFTTTLESQGDISDGVLRGNLIARFSGDPTFKRQSLRNMVAILKKQGVRQITGDVLVDTSVFASHDKAPGWPWNDLTQCF
SAPPAAAIVDRNCFSVSLYSAPNPGDMAFIRVASYYPVNMFSQVRTLAKGSADAQYCELDVVPGELNRFTLTGCLTQRSD
PLPLAFAIQDGASYAGAILKDELTQAGIQIDGHLKRQTQPGLKGTVIAQAQSAPLHELLKIMLKKSDNMIADTVFRTIGH
ERFGVPGTWRAGADAVRQVLRQKAGVDLGNSIVVDGSGLSRHNLLAPATMMQALQYIAQHDSELNFISMLPLSGYDGTLR
YRGGLHEAGVDGKVSAKTGALQGVYNLAGFITTASGQRFAFVQYLSGYAVPPEDQKQRRAPLVRFESRLYRDIYQNN

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=477, Percent_Identity=79.4549266247379, Blast_Score=806, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 51750; Mature: 51750

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSRIVSALACAFVLNANAAPVEDYTQYLPDGANLALVVQKIGADAPTIDYHSQQMALP
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEEHHHCCCCCCCCCCCCCEECC
ASTQKVLTALAALLQLGPDYRFTTTLESQGDISDGVLRGNLIARFSGDPTFKRQSLRNMV
CHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEECCEEEEECCCCCHHHHHHHHHH
AILKKQGVRQITGDVLVDTSVFASHDKAPGWPWNDLTQCFSAPPAAAIVDRNCFSVSLYS
HHHHHCCHHHHCCCEEEECHHHHCCCCCCCCCHHHHHHHHCCCCCHHEECCCCEEEEEEC
APNPGDMAFIRVASYYPVNMFSQVRTLAKGSADAQYCELDVVPGELNRFTLTGCLTQRSD
CCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEECCCCCC
PLPLAFAIQDGASYAGAILKDELTQAGIQIDGHLKRQTQPGLKGTVIAQAQSAPLHELLK
CCCEEEEEECCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCCCEEEEECCCCCCHHHHHH
IMLKKSDNMIADTVFRTIGHERFGVPGTWRAGADAVRQVLRQKAGVDLGNSIVVDGSGLS
HHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCC
RHNLLAPATMMQALQYIAQHDSELNFISMLPLSGYDGTLRYRGGLHEAGVDGKVSAKTGA
CCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCCCCCCCCCCEEECCHHH
LQGVYNLAGFITTASGQRFAFVQYLSGYAVPPEDQKQRRAPLVRFESRLYRDIYQNN
HHHHHHHHHHEEECCCCEEEHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRFSRIVSALACAFVLNANAAPVEDYTQYLPDGANLALVVQKIGADAPTIDYHSQQMALP
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEEHHHCCCCCCCCCCCCCEECC
ASTQKVLTALAALLQLGPDYRFTTTLESQGDISDGVLRGNLIARFSGDPTFKRQSLRNMV
CHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEECCEEEEECCCCCHHHHHHHHHH
AILKKQGVRQITGDVLVDTSVFASHDKAPGWPWNDLTQCFSAPPAAAIVDRNCFSVSLYS
HHHHHCCHHHHCCCEEEECHHHHCCCCCCCCCHHHHHHHHCCCCCHHEECCCCEEEEEEC
APNPGDMAFIRVASYYPVNMFSQVRTLAKGSADAQYCELDVVPGELNRFTLTGCLTQRSD
CCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEECCCCCC
PLPLAFAIQDGASYAGAILKDELTQAGIQIDGHLKRQTQPGLKGTVIAQAQSAPLHELLK
CCCEEEEEECCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCCCEEEEECCCCCCHHHHHH
IMLKKSDNMIADTVFRTIGHERFGVPGTWRAGADAVRQVLRQKAGVDLGNSIVVDGSGLS
HHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCC
RHNLLAPATMMQALQYIAQHDSELNFISMLPLSGYDGTLRYRGGLHEAGVDGKVSAKTGA
CCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCCCCCCCCCCEEECCHHH
LQGVYNLAGFITTASGQRFAFVQYLSGYAVPPEDQKQRRAPLVRFESRLYRDIYQNN
HHHHHHHHHHEEECCCCEEEHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]