Click here to switch to the map view.

The map label for this gene is wcaM [H]

Identifier: 157159381

GI number: 157159381

Start: 2297425

End: 2298819

Strand: Reverse

Name: wcaM [H]

Synonym: EcE24377A_2335

Alternate gene names: 157159381

Gene position: 2298819-2297425 (Counterclockwise)

Preceding gene: 157158914

Following gene: 157158858

Centisome position: 46.16

GC content: 47.53

Gene sequence:

>1395_bases
ATGCCATTTAAAAAACTCTCCCGACGCACCTTCCTGACGGCAAGCTCGGCGCTTGCCTTCCTCCATACCCCTTTCGCTCG
CGCACTTCCCGCCCGACAAAGCGTTAACATTAACGACTACAACCCACACGACTGGATCGCCTCATTTAAACAAGCCTTCA
GCGAAGGGCAAACAGTCGTCGTGCCTGCCGGATTGGTTTGTGACAATATCAACACCGGCATCTTCATTCCTCCAGGCAAA
ACGTTACACATCCTGGGAAGCCTGCGCGGTAACGGCAGAGGGCGTTTTGTCTTACAGGACGGCAGCCAGGTGACAGGGAA
GGAGGGCGGCGGTATGCATAACATCACCCTGGATGTGCGTGGCTCTGACTGCACCATCAAAGGGCTGACGATGAGCGGCT
TTGGTCCGGTAACGCAGATTTATATCGGCGGCAAAAACAAACGGGTCATGCGCAACCTGACCATCGATAACCTCACTGTC
AGCCACGCTAATTACGCCATCTTACGCCAGGGATTTCATAACCAGATAATCGGTGCCAACATCACCAACTGTAAATTTAG
TGATTTACAGGGCGACGCCATTGAATGGAACGTGGCGATTAACGACCGTGATATCTTGATCTCCGACCATGTCATCGAGC
GCATCAACTGTACTAATGGCAAAATCAACTGGGGCATCGGCATAGGTCTTGCGGGAAGTACCTACGATAACAACTACCCG
GAAGACCAGGCAGTGAAAAACTTTGTCGTGGCGAATATCACGGGATCGGATTGTCGGCAGTTGATCCATGTTGAAAATGG
TAAACATTTTGTTATTCGTAATATCAAAGCCCGCAATATCACGCCGGATTTTAGTAAGAAAGCAGGTATTGATAACGCCA
CAGTCGCTATTTACGGTTGTGACAATTTCGTGATTGATAATATTGAAATGACTAATAGCGCCGGGATGTTAATCGGTTAT
GGGGTAATTAAAGGCAAATATCTCTCGATACCGCAAAATTTCCGAGTGAATAATATTCAACTGGATAACACCCATCTTGC
TTATAAATTGCGCGGCATCCAAATCTCCGCCGGGAATGCTGTCTCCTTTGTGGCACTGACTAACATTGAGATGAAGCGTG
CATCGCTGGAGTTACACAACAAACCGCAACATCTTTTTATGCGTAATATCAAGGTGATGCAGGAATCCTCAGTTGGACCA
GCATTGAGCATGAACTTCGACATGCGCAAAGACGTTCGCGGCGTCTTTATGGCGAAAAAAGAAACACTGCTGTCTCTTGC
AAATGTTCATGCGGTGAATGAAAAAGGGCAAAGCTCCGTCGATATCGACAGAGTTAATCACCATATTGTTAATGTGGAAA
AGATTAACTTTAGATTGCCGGAACGGAGAGAGTAG

Upstream 100 bases:

>100_bases
GTTGTCAAACGTGCGCGCGAAAAAGTCGAACACGATTTTAACCAGCAGGTGATTAATCGAGAACTCGCCAGCTTGTTACA
GGCTTTATAGAGGAGAACGT

Downstream 100 bases:

>100_bases
ATTTGCGACCATTCCTGGAAAAATGGAGCCATACTTAGGAACAATGCTACTGCAATCCACAACGAAGCGGCGTAACATCA
CAAGTAATTCAGTAATCAAT

Product: putative colanic acid biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHDWIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGK
TLHILGSLRGNGRGRFVLQDGSQVTGKEGGGMHNITLDVRGSDCTIKGLTMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYP
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMTNSAGMLIGY
GVIKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKVMQESSVGP
ALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIVNVEKINFRLPERRE

Sequences:

>Translated_464_residues
MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHDWIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGK
TLHILGSLRGNGRGRFVLQDGSQVTGKEGGGMHNITLDVRGSDCTIKGLTMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYP
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMTNSAGMLIGY
GVIKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKVMQESSVGP
ALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIVNVEKINFRLPERRE
>Mature_463_residues
PFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHDWIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKT
LHILGSLRGNGRGRFVLQDGSQVTGKEGGGMHNITLDVRGSDCTIKGLTMSGFGPVTQIYIGGKNKRVMRNLTIDNLTVS
HANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYPE
DQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMTNSAGMLIGYG
VIKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKVMQESSVGPA
LSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIVNVEKINFRLPERRE

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788356, Length=464, Percent_Identity=97.8448275862069, Blast_Score=937, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011050 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 51283; Mature: 51152

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHDWIASFKQAFSEGQTVV
CCHHHHCCEEEEEHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEE
VPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGSQVTGKEGGGMHNITLDVR
EECCEEECCCCCCEEECCCCEEEEEEECCCCCCEEEEEECCCEECCCCCCCEEEEEEEEE
GSDCTIKGLTMSGFGPVTQIYIGGKNKRVMRNLTIDNLTVSHANYAILRQGFHNQIIGAN
CCCEEEEEEEECCCCCEEEEEECCCCCEEEEEEEECEEEECCCCHHHHHCCCCCCCCCCC
ITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYP
CCCCEECCCCCCEEEEEEEECCCEEEEECCHHHEEECCCCEEEEEEEEEECCCCCCCCCC
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGC
CHHHHCEEEEEECCCHHHHEEEEECCCCEEEEEEEEECCCCCCCHHHCCCCCCEEEEEEC
DNFVIDNIEMTNSAGMLIGYGVIKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNA
CCEEEECEEECCCCCEEEEEEEECCEEECCCCCCEEEEEEECCCEEEEEEEEEEEECCCE
VSFVALTNIEMKRASLELHNKPQHLFMRNIKVMQESSVGPALSMNFDMRKDVRGVFMAKK
EEEEEEECEEEEEEEEEECCCCHHHHHHHHHHEECCCCCCEEEECCCHHHCCCEEEEEHH
ETLLSLANVHAVNEKGQSSVDIDRVNHHIVNVEKINFRLPERRE
HHHHHHHHEEEECCCCCCCCCHHHCCEEEEEEEEEEEECCCCCC
>Mature Secondary Structure 
PFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHDWIASFKQAFSEGQTVV
CHHHHCCEEEEEHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEE
VPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGSQVTGKEGGGMHNITLDVR
EECCEEECCCCCCEEECCCCEEEEEEECCCCCCEEEEEECCCEECCCCCCCEEEEEEEEE
GSDCTIKGLTMSGFGPVTQIYIGGKNKRVMRNLTIDNLTVSHANYAILRQGFHNQIIGAN
CCCEEEEEEEECCCCCEEEEEECCCCCEEEEEEEECEEEECCCCHHHHHCCCCCCCCCCC
ITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYP
CCCCEECCCCCCEEEEEEEECCCEEEEECCHHHEEECCCCEEEEEEEEEECCCCCCCCCC
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPDFSKKAGIDNATVAIYGC
CHHHHCEEEEEECCCHHHHEEEEECCCCEEEEEEEEECCCCCCCHHHCCCCCCEEEEEEC
DNFVIDNIEMTNSAGMLIGYGVIKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNA
CCEEEECEEECCCCCEEEEEEEECCEEECCCCCCEEEEEEECCCEEEEEEEEEEEECCCE
VSFVALTNIEMKRASLELHNKPQHLFMRNIKVMQESSVGPALSMNFDMRKDVRGVFMAKK
EEEEEEECEEEEEEEEEECCCCHHHHHHHHHHEECCCCCCEEEECCCHHHCCCEEEEEHH
ETLLSLANVHAVNEKGQSSVDIDRVNHHIVNVEKINFRLPERRE
HHHHHHHHEEEECCCCCCCCCHHHCCEEEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]