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The map label for this gene is wcaL [H]

Identifier: 157158914

GI number: 157158914

Start: 2298830

End: 2300050

Strand: Reverse

Name: wcaL [H]

Synonym: EcE24377A_2336

Alternate gene names: 157158914

Gene position: 2300050-2298830 (Counterclockwise)

Preceding gene: 157156059

Following gene: 157159381

Centisome position: 46.19

GC content: 54.3

Gene sequence:

>1221_bases
ATGAAGGTCGGCTTCTTTTTACTGAAATTTCCGCTGTCGTCGGAAACCTTCGTTCTTAACCAAATTACCGCGTTTATTGA
TATGGGATTCGAGGTGGAGATTGTTGCGCTGCAAAAAGGCGACACCCAGAACACCCACGCGGCATGGACGAAATACAACC
TTGCCGCAAGAACCCGCTGGTTACAGGACGAACCACAAGGCAAAGTGGCGAAACTGCGCCACCGCGCCAGCCAGACCTTA
CGCGGCATTCATCGTAAAAATACCTGGCAGGCGCTCAATCTCAAACGCTATGGTGCCGAGTCGCGGAACCTGATTTTGTC
TGCCATTTGCGGTCAGGTCGCAACACCGTTTCATGCCGATGTCTTTATCGCTCATTTTGGTCCTGCGGGGGTAGCCGCAG
CAAAACTACGCGAACTGGGTGTCATTCGCGGCAAAATTGCCACTATCTTCCACGGTATTGATATCTCCAGTCGGGAAGTG
CTCAACCACTACACTCCCGAATATCAGCAACTGTTTTGCCGTGGCGACCTGATGTTACCGATAAGCGATCTGTGGGCCGG
AAGGCTGCAAAAAATGGGCTGCCCGAGGGAAAAAATCGCCGTATCGCGCATGGGCGTAGATATGACGCGCTTTAGCCCGC
GTCCCGTGAAAGCGCCCGCAACGCCGCTGGAAATCATCTCCGTCGCACGTTTAACCGAGAAAAAAGGCCTGCATGTGGCG
ATTGAAGCCTGCCGTCAGTTGAAAGAGCAGGGCGTGGCATTTCGCTATCGCATCCTCGGCATTGGCCCGTGGGAACGACG
CCTGCGCACGCTCATCGAACAATATCAACTGGAAGATGTGGTGGAGATGCCTGGCTTTAAACCGAGCCATGAAGTGAAAG
CGATGCTCGACGACGCGGATGTCTTCCTGTTGCCATCGGTTACAGGTGCGGATGGTGATATGGAAGGCATTCCGGTGGCG
CTAATGGAAGCGATGGCGGTCGGCATTCCGGTGGTTTCTACTCTGCATAGCGGAATACCGGAACTGGTGGAGGCTGACAA
ATCCGGCTGGCTGGTGCCTGAGAACGATGCTCGCGCACTGGCGCAACGACTGGCGGCGTTTAGCCAACTGGACACCGACG
AACTGGCTCCGGTTGTCAAACGTGCGCGCGAAAAAGTCGAACACGATTTTAACCAGCAGGTGATTAATCGAGAACTCGCC
AGCTTGTTACAGGCTTTATAG

Upstream 100 bases:

>100_bases
TTCCGGTGCTGAACGCTCGACTTAACGAAGCCGTCAGTCGCGAACGTCAGACGGGAATGCAGATGGTGCAATCTGTGCTT
GAACGCATCGGGGAGGTGAA

Downstream 100 bases:

>100_bases
AGGAGAACGTATGCCATTTAAAAAACTCTCCCGACGCACCTTCCTGACGGCAAGCTCGGCGCTTGCCTTCCTCCATACCC
CTTTCGCTCGCGCACTTCCC

Product: colanic acid biosynthesis glycosyl transferase WcaL

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQNTHAAWTKYNLAARTRWLQDEPQGKVAKLRHRASQTL
RGIHRKNTWQALNLKRYGAESRNLILSAICGQVATPFHADVFIAHFGPAGVAAAKLRELGVIRGKIATIFHGIDISSREV
LNHYTPEYQQLFCRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKAPATPLEIISVARLTEKKGLHVA
IEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQLEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVA
LMEAMAVGIPVVSTLHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQQVINRELA
SLLQAL

Sequences:

>Translated_406_residues
MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQNTHAAWTKYNLAARTRWLQDEPQGKVAKLRHRASQTL
RGIHRKNTWQALNLKRYGAESRNLILSAICGQVATPFHADVFIAHFGPAGVAAAKLRELGVIRGKIATIFHGIDISSREV
LNHYTPEYQQLFCRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKAPATPLEIISVARLTEKKGLHVA
IEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQLEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVA
LMEAMAVGIPVVSTLHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQQVINRELA
SLLQAL
>Mature_406_residues
MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQNTHAAWTKYNLAARTRWLQDEPQGKVAKLRHRASQTL
RGIHRKNTWQALNLKRYGAESRNLILSAICGQVATPFHADVFIAHFGPAGVAAAKLRELGVIRGKIATIFHGIDISSREV
LNHYTPEYQQLFCRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKAPATPLEIISVARLTEKKGLHVA
IEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQLEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVA
LMEAMAVGIPVVSTLHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQQVINRELA
SLLQAL

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=406, Percent_Identity=98.7684729064039, Blast_Score=823, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 45273; Mature: 45273

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQNTHAAWTKYNLAARTRW
CCCEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHH
LQDEPQGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESRNLILSAICGQVATPFHAD
CCCCCCCHHHHHHHHHHHHHHHHHHCCCHHEECHHHCCCCHHHHHHHHHHHHHCCCCCCE
VFIAHFGPAGVAAAKLRELGVIRGKIATIFHGIDISSREVLNHYTPEYQQLFCRGDLMLP
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHCCCCCCC
ISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKAPATPLEIISVARLTEKKGLHVA
HHHHHHHHHHHCCCCHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHH
IEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQLEDVVEMPGFKPSHEVKAMLDDAD
HHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
VFLLPSVTGADGDMEGIPVALMEAMAVGIPVVSTLHSGIPELVEADKSGWLVPENDARAL
EEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHH
AQRLAAFSQLDTDELAPVVKRAREKVEHDFNQQVINRELASLLQAL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQNTHAAWTKYNLAARTRW
CCCEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHH
LQDEPQGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESRNLILSAICGQVATPFHAD
CCCCCCCHHHHHHHHHHHHHHHHHHCCCHHEECHHHCCCCHHHHHHHHHHHHHCCCCCCE
VFIAHFGPAGVAAAKLRELGVIRGKIATIFHGIDISSREVLNHYTPEYQQLFCRGDLMLP
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHCCCCCCC
ISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKAPATPLEIISVARLTEKKGLHVA
HHHHHHHHHHHCCCCHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHH
IEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQLEDVVEMPGFKPSHEVKAMLDDAD
HHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
VFLLPSVTGADGDMEGIPVALMEAMAVGIPVVSTLHSGIPELVEADKSGWLVPENDARAL
EEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHH
AQRLAAFSQLDTDELAPVVKRAREKVEHDFNQQVINRELASLLQAL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]