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The map label for this gene is galF [H]

Identifier: 157158858

GI number: 157158858

Start: 2296357

End: 2297250

Strand: Reverse

Name: galF [H]

Synonym: EcE24377A_2334

Alternate gene names: 157158858

Gene position: 2297250-2296357 (Counterclockwise)

Preceding gene: 157159381

Following gene: 157159205

Centisome position: 46.13

GC content: 50.34

Gene sequence:

>894_bases
ATGACGAATTTAAAAGCAGTTATTCCTGTAGCGGGTCTTGGGATGCATATGTTGCCTGCCACTAAGGCGATTCCCAAAGA
GATGCTACCGATCGTCGACAAGCCAATGATTCAGTATATTGTTGACGAGATTGTGGCTGCAGGGATCAAAGAAATCCTCC
TGGTAACTCATGCGTCCAAGAACGCGGTCGAAAACCACTTCGACACCTCTTATGAATTAGAATCTCTTCTTGAACTGCGC
GTGAAGCGTCAACTACTGGCGGAAGTTCAGTCCATCTGCCCGCCGGGCGTGACCATTATGAACGTCCGTCAGGGCGAACC
TTTAGGTTTAGGTCACTCCATTTTGTGTGCACGACCCGCCATTGGTGACAACCCATTTGTCGTGGTGCTGCCAGACGTTG
TGATCGACGACGCCAGTGCCGATCCGCTGCGTTACAACCTTGCTGCCATGATTGCGCGTTTCAATGAAACGGGCCGTAGC
CAGGTGCTGGCAAAACGTATGCCGGGTGACCTCTCTGAATACTCCGTTATTCAGACCAAAGAACCACTGGATCGTGAAGG
TAAAGTCAGCCGCATTGTTGAATTTATCGAAAAACCGGATCAGCCGCAGACGCTGGACTCAGACATCATGGCCGTGGGCC
GTTATGTGCTTTCTGCTGATATTTGGCCGGAACTAGAACGCACTCAGCCTGGTGCATGGGGGCGTATTCAGCTGACTGAT
GCCATCGCTGAACTGGCGAAAAAACAGTCCGTTGATGCCATGCTGATGACTGGAGACAGCTACGACTGCGGTAAAAAAAT
GGGTTATATGCAGGCATTTGTGAAGTATGGACTACGCAACCTGAAAGAAGGGGCGAAGTTCCGTAAAGGGATTGAGAAGC
TGTTAAGCGAATAA

Upstream 100 bases:

>100_bases
ACATCACAAGTAATTCAGTAATCAATTCAGGGTAATTGATGCTGGCGAAAAAAATCGAACAAGCTATAATTCAGCAACCA
TTTTACAGGTGGATGAAATA

Downstream 100 bases:

>100_bases
TGAAAATCTGACCGGATGTAACGGTTGATAAGAAAATTATAACGGCAGTGAAGATTCGTGGCGAAAGTAATTTGTTGCGA
ATATTCCTGCCGTTGTTTTA

Product: UTP--glucose-1-phosphate uridylyltransferase subunit GalF

Products: NA

Alternate protein names: Alpha-D-glucosyl-1-phosphate uridylyltransferase; UDP-glucose pyrophosphorylase; UDPGP; Uridine diphosphoglucose pyrophosphorylase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLELR
VKRQLLAEVQSICPPGVTIMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLSADIWPELERTQPGAWGRIQLTD
AIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKYGLRNLKEGAKFRKGIEKLLSE

Sequences:

>Translated_297_residues
MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLELR
VKRQLLAEVQSICPPGVTIMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLSADIWPELERTQPGAWGRIQLTD
AIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKYGLRNLKEGAKFRKGIEKLLSE
>Mature_296_residues
TNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLELRV
KRQLLAEVQSICPPGVTIMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRSQ
VLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLSADIWPELERTQPGAWGRIQLTDA
IAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKYGLRNLKEGAKFRKGIEKLLSE

Specific function: Lipopolysaccharide biosynthesis. [C]

COG id: COG1210

COG function: function code M; UDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDPGP type 2 family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=288, Percent_Identity=23.9583333333333, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI11761619, Length=288, Percent_Identity=23.9583333333333, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1788355, Length=297, Percent_Identity=99.6632996632997, Blast_Score=607, Evalue=1e-175,
Organism=Escherichia coli, GI1787488, Length=289, Percent_Identity=58.477508650519, Blast_Score=340, Evalue=5e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005774
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.9 [H]

Molecular weight: Translated: 32815; Mature: 32684

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEIVAAGIKEILLVTHASK
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCH
NAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNVRQGEPLGLGHSILCARPA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHEEECCC
IGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRSQVLAKRMPGDLSEYSVIQTK
CCCCCEEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEECCC
EPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLSADIWPELERTQPGAWGRIQLTD
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCEEEHHH
AIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKYGLRNLKEGAKFRKGIEKLLSE
HHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEIVAAGIKEILLVTHASK
CCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCH
NAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNVRQGEPLGLGHSILCARPA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHEEECCC
IGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRSQVLAKRMPGDLSEYSVIQTK
CCCCCEEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEECCC
EPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLSADIWPELERTQPGAWGRIQLTD
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCEEEHHH
AIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKYGLRNLKEGAKFRKGIEKLLSE
HHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]