Click here to switch to the map view.

The map label for this gene is fimF

Identifier: 157163762

GI number: 157163762

Start: 4552750

End: 4553280

Strand: Direct

Name: fimF

Synonym: EcHS_A4544

Alternate gene names: 157163762

Gene position: 4552750-4553280 (Clockwise)

Preceding gene: 157163761

Following gene: 157163763

Centisome position: 98.04

GC content: 49.15

Gene sequence:

>531_bases
ATGAGAAACAAACCTTTTTATCTTCTGTGCGCTTTTTTGTGGCTGGCGGTGAGTCACGCTTTGGCTGCGGATAGCACGAT
TACTATCCGCGGCTATGTCAGGGATAACGGCTGTAGTGTGGCCGCTGAATCAACCAATTTTACTGTTGATCTGATGGAAA
ACGCGGCGAAGCAATTTAACAACATTGGCGCGACGACTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAAT
GCCGTTTCTGCCGTAAAAGTTGGGTTTACCGGCGTTGCAGATAGCCACAATGCCAATCTGCTTGCACTTGAAAATACGGT
GTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTAATGCTCCATCGTCCGCGC
TTTCGTGGACGACCCTGACGCCGGGTAAACCAAATACGCTGAATTTTTACGCCCGGCTAATGGCGACACAGGTGCCTGTC
ACTGCAGGGCATATCAATGCCACGGCTACCTTCACTCTTGAATATCAGTAA

Upstream 100 bases:

>100_bases
AGAGGAAAATGCTCACTGTGTCGCCAATTATCAACTGCCACCAGAGAGGCAGCAGCAGTTATTAACCCAGCTATCAGCTG
AATGTCGTTAAGGGGGCGTG

Downstream 100 bases:

>100_bases
CTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGC
AGCCGATGTCACCATCACGG

Product: fimbrial protein FimF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAESTNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGN
AVSAVKVGFTGVADSHNANLLALENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
TAGHINATATFTLEYQ

Sequences:

>Translated_176_residues
MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAESTNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGN
AVSAVKVGFTGVADSHNANLLALENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
TAGHINATATFTLEYQ
>Mature_176_residues
MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAESTNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGN
AVSAVKVGFTGVADSHNANLLALENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
TAGHINATATFTLEYQ

Specific function: Involved in regulation of length and mediation of adhesion of type 1 fimbriae (but not necessary for the production of fimbriae). Involved in the integration of fimH in the fimbriae

COG id: COG3539

COG function: function code NU; P pilus assembly protein, pilin FimA

Gene ontology:

Cell location: Fimbrium

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fimbrial protein family

Homologues:

Organism=Escherichia coli, GI1790773, Length=176, Percent_Identity=100, Blast_Score=359, Evalue=1e-101,
Organism=Escherichia coli, GI1787781, Length=171, Percent_Identity=45.0292397660819, Blast_Score=149, Evalue=9e-38,
Organism=Escherichia coli, GI87081805, Length=156, Percent_Identity=37.8205128205128, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI87082421, Length=185, Percent_Identity=30.8108108108108, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI87081753, Length=163, Percent_Identity=33.1288343558282, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1786746, Length=179, Percent_Identity=32.9608938547486, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1787174, Length=186, Percent_Identity=28.494623655914, Blast_Score=61, Evalue=4e-11,
Organism=Escherichia coli, GI1790769, Length=154, Percent_Identity=34.4155844155844, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FIMF_ECOLI (P08189)

Other databases:

- EMBL:   X05672
- EMBL:   U14003
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   X51655
- PIR:   S56543
- RefSeq:   AP_004810.1
- RefSeq:   NP_418738.1
- PDB:   2JMR
- PDB:   3BFQ
- PDB:   3BFW
- PDB:   3BWU
- PDB:   3JWN
- PDBsum:   2JMR
- PDBsum:   3BFQ
- PDBsum:   3BFW
- PDBsum:   3BWU
- PDBsum:   3JWN
- ProteinModelPortal:   P08189
- SMR:   P08189
- MINT:   MINT-6491069
- STRING:   P08189
- EnsemblBacteria:   EBESCT00000004548
- EnsemblBacteria:   EBESCT00000015743
- GeneID:   948845
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW4281
- KEGG:   eco:b4318
- EchoBASE:   EB0309
- EcoGene:   EG10313
- eggNOG:   COG3539
- GeneTree:   EBGT00050000008609
- HOGENOM:   HBG701898
- ProtClustDB:   CLSK880032
- BioCyc:   EcoCyc:EG10313-MONOMER
- Genevestigator:   P08189
- GO:   GO:0009289
- InterPro:   IPR008966
- InterPro:   IPR000259
- InterPro:   IPR014779
- Gene3D:   G3DSA:2.60.40.1090

Pfam domain/function: PF00419 Fimbrial; SSF49401 Adhes_bact

EC number: NA

Molecular weight: Translated: 18715; Mature: 18715

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAESTNFTVDLMENAAKQFN
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEECCCCEEEHHHHHHHHHHH
NIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLALENTVSAASGLGIQLLNE
CCCCCCCCHHHHHEECCCCCCEEEEEECEEECCCCCCCCEEEEECCCCCCCCCCEEEECC
QQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPVTAGHINATATFTLEYQ
CCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCEEECCCCEEEEEEEEEC
>Mature Secondary Structure
MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAESTNFTVDLMENAAKQFN
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEECCCCEEEHHHHHHHHHHH
NIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLALENTVSAASGLGIQLLNE
CCCCCCCCHHHHHEECCCCCCEEEEEECEEECCCCCCCCEEEEECCCCCCCCCCEEEECC
QQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPVTAGHINATATFTLEYQ
CCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCEEECCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2890081; 7610040; 9278503; 1970114