Click here to switch to the map view.

The map label for this gene is fimE

Identifier: 157163757

GI number: 157163757

Start: 4547040

End: 4547636

Strand: Direct

Name: fimE

Synonym: EcHS_A4539

Alternate gene names: 157163757

Gene position: 4547040-4547636 (Clockwise)

Preceding gene: 157163756

Following gene: 157163758

Centisome position: 97.92

GC content: 46.9

Gene sequence:

>597_bases
GTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTACGGGGCAACGGGAGCCAG
AGATTATTGTCTTATTCTGTTGGCATATCGGCATGGGATGCGTATTAGTGAACTGCTTGATCTGCATTATCAGGACCTTG
ACCTTAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGT
GAAGCCGTGGAACGATGGACCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCG
CGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTC
ATCCTCATATGTTAAGGCATGCTTGCGGTTATGAACTGGCGGAGCGTGGTGCCGATACCCGTTTAATTCAGGATTATCTC
GGGCATCGAAATATTCGCCATACTGTGCGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAA
TCTCATAAACGAAAAATTAAAAAGAGAAGAAGTTTAA

Upstream 100 bases:

>100_bases
CCGTGTGTGGTTATCTTTTTATCTATTGGGCTAATTTTGACCGATTGAGGTTTCCTATAGGTATTCATTCAAATATATCT
CAGTTAGGAGTACTACTATT

Downstream 100 bases:

>100_bases
TTTAACTTATTGATAATAAAGTTAAAAAGCAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCATAAATAAGTT
ACGTATTTTTTCTCAAGCAT

Product: tyrosine recombinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISELLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDER
EAVERWTQERANWKGADRTDAIFISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV

Sequences:

>Translated_198_residues
MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISELLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDER
EAVERWTQERANWKGADRTDAIFISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV
>Mature_197_residues
SKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISELLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDERE
AVERWTQERANWKGADRTDAIFISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYLG
HRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV

Specific function: FimE is one of the 2 regulatory proteins which control the phase variation of type 1 fimbriae in E.coli. These proteins mediate the periodic inversion of a 300bp DNA segment that harbors the promoter for the fimbrial structural gene, fimA. FimE switches f

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family

Homologues:

Organism=Escherichia coli, GI1790768, Length=198, Percent_Identity=100, Blast_Score=408, Evalue=1e-115,
Organism=Escherichia coli, GI1790767, Length=183, Percent_Identity=52.4590163934426, Blast_Score=198, Evalue=2e-52,
Organism=Escherichia coli, GI1790244, Length=167, Percent_Identity=29.940119760479, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1789261, Length=184, Percent_Identity=27.7173913043478, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FIME_ECOL6 (P0ADH8)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_757240.1
- ProteinModelPortal:   P0ADH8
- SMR:   P0ADH8
- EnsemblBacteria:   EBESCT00000043640
- GeneID:   1037249
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c5392
- GeneTree:   EBGT00050000008629
- HOGENOM:   HBG287305
- OMA:   TDALFIS
- ProtClustDB:   PRK09871
- GO:   GO:0006350
- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- Gene3D:   G3DSA:1.10.443.10

Pfam domain/function: PF00589 Phage_integrase; SSF56349 DNA_brk_join_enz

EC number: NA

Molecular weight: Translated: 23117; Mature: 22985

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: ACT_SITE 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISELLDLHYQDLDLNEGRI
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCC
NIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAIFISRRGSRLSRQQAYRII
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHH
RDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYLGHRNIRHTVRYTASNAARFA
HHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHCCHHHHH
GLWERNNLINEKLKREEV
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISELLDLHYQDLDLNEGRI
CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCC
NIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAIFISRRGSRLSRQQAYRII
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHH
RDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYLGHRNIRHTVRYTASNAARFA
HHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHCCHHHHH
GLWERNNLINEKLKREEV
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157