Click here to switch to the map view.

The map label for this gene is fimB

Identifier: 157163756

GI number: 157163756

Start: 4545960

End: 4546562

Strand: Direct

Name: fimB

Synonym: EcHS_A4538

Alternate gene names: 157163756

Gene position: 4545960-4546562 (Clockwise)

Preceding gene: 157163745

Following gene: 157163757

Centisome position: 97.9

GC content: 43.45

Gene sequence:

>603_bases
ATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCCAATAC
CGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGAT
TGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATTAAAAAAAGGCTTTTCAACAACGCATCCG
CTATTGAACAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGT
ATTTTTATCTCGTAAGGGAAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATGCCGGGT
TGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTT
ATCCAGGATTATCTTGGGCATCGCAATATTCGTCATACTGTCTGGTATACCGCCAGCAATGCAGGGCGTTTTTACGGCAT
CTGGGATAGAGCCAGAGGACGACAGCGTCACGCTGTTTTATAG

Upstream 100 bases:

>100_bases
AAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCT
GTAATTATAAGGGAAAAACG

Downstream 100 bases:

>100_bases
AGATAGTTGAGATACCAGGGATGGTGTTAAAAACTACATTATTCTTACGATATATCAAAAATGATTAACCATTTATTAAT
TGATGTTATTGTAAGTGTGA

Product: tyrosine recombinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIHGFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHP
LLNKEVQALKNWLSIRTSYPHAESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL

Sequences:

>Translated_200_residues
MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIHGFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHP
LLNKEVQALKNWLSIRTSYPHAESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL
>Mature_200_residues
MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIHGFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHP
LLNKEVQALKNWLSIRTSYPHAESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL

Specific function: FimB is one of the 2 regulatory proteins which control the phase variation of type 1 fimbriae in E.coli. These proteins mediate the periodic inversion of a 300bp DNA segment that harbors the promoter for the fimbrial structural gene, fimA. FimB switches f

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family

Homologues:

Organism=Escherichia coli, GI1790767, Length=200, Percent_Identity=100, Blast_Score=416, Evalue=1e-118,
Organism=Escherichia coli, GI1790768, Length=183, Percent_Identity=52.4590163934426, Blast_Score=198, Evalue=2e-52,
Organism=Escherichia coli, GI1790244, Length=182, Percent_Identity=26.9230769230769, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1789261, Length=170, Percent_Identity=29.4117647058824, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FIMB_ECO57 (P0ADH6)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B86129
- PIR:   G91287
- RefSeq:   NP_290928.1
- RefSeq:   NP_313298.1
- ProteinModelPortal:   P0ADH6
- SMR:   P0ADH6
- MINT:   MINT-1221125
- EnsemblBacteria:   EBESCT00000028809
- EnsemblBacteria:   EBESCT00000059768
- GeneID:   913695
- GeneID:   959741
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5910
- KEGG:   ecs:ECs5271
- GeneTree:   EBGT00050000008629
- HOGENOM:   HBG287305
- OMA:   FIHGLRA
- ProtClustDB:   PRK09870
- BioCyc:   ECOL83334:ECS5271-MONOMER
- GO:   GO:0006350
- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- Gene3D:   G3DSA:1.10.443.10

Pfam domain/function: PF00589 Phage_integrase; SSF56349 DNA_brk_join_enz

EC number: NA

Molecular weight: Translated: 22994; Mature: 22994

Theoretical pI: Translated: 10.69; Mature: 10.69

Prosite motif: NA

Important sites: ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIHGFRASEICRLRISDID
CCCCCCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
LKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHAESEWVFLSRKGNPLSRQQ
CEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHH
FYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRLIQDYLGHRNIRHTVWYTASN
HEEHEECCCCCCCEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEEECC
AGRFYGIWDRARGRQRHAVL
CCCEEEEHHHCCCCCCCCCC
>Mature Secondary Structure
MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIHGFRASEICRLRISDID
CCCCCCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
LKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHAESEWVFLSRKGNPLSRQQ
CEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHH
FYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRLIQDYLGHRNIRHTVWYTASN
HEEHEECCCCCCCEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEEECC
AGRFYGIWDRARGRQRHAVL
CCCEEEEHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796