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The map label for this gene is gspJ2 [H]

Identifier: 157162432

GI number: 157162432

Start: 3142196

End: 3142801

Strand: Reverse

Name: gspJ2 [H]

Synonym: EcHS_A3132

Alternate gene names: 157162432

Gene position: 3142801-3142196 (Counterclockwise)

Preceding gene: 157162433

Following gene: 157162431

Centisome position: 67.68

GC content: 57.59

Gene sequence:

>606_bases
GTGAGAAGGGCTCGCGCTGGTTTCACGTTACTGGAAATGCTGGTGGCAATTGCCATTTTTGCCTCGCTGGCACTGATGGC
GCAGCAGGTGACTAACGGCGTCACGCGCGTGAATAGCGCCGTCGCCGGACACGATCAAAAACTAAACCTCATGCAGCAAA
CGATGAGTTTTCTGACCCACGATCTGACCCAAATGATGCCGCGTCCGGTAAGAGGCGATCAGGGCCAGCGTGAACCAGCG
TTACTGGCTGGCGCTGGCGTGCTGGCGTCTGAGAGTGAAGGAATGCGTTTTGTGCGCGGCGGTGTGGTTAACCCGTTGAT
GCGTATGCCACGCAGCAATCTGCTCACCGTCGGTTACCGCATTCATGACGGTTATCTCGAACGGTTGTCCTGGCCGCTGA
CCGATGCCGCAGGCAGCGTGAAGCCGACAATGCAAAAGTTGATTCCGGCCGATTCGCTCCATTTGCAGTTCTACGACGGC
ACGCGCTGGCAGGAGAGCTGGTCGTCAGTACAGGCGATCCCTGTGGCAGTGCGCATGACTCTGCATTCGCCGCAATGGGG
CGAGATTGAACGCATCTGGTTGTTACGCGGGCCGCAATTATCATGA

Upstream 100 bases:

>100_bases
CGCTGCTCCAGGCGGTGGATATTGAAGTCAGCCTTCACGAAGACTTTTCGCCGGTGATTCAGTCACGACGCGCCTGGTTT
AGCGCCGTGGGAGGCCAACA

Downstream 100 bases:

>100_bases
TCACCTCACCACCAAAACGCGGAATGGCACTGGTCGTGGTGCTGGTATTGCTGGCAGTTATGATGCTGGTAACCATCACG
CTTTCCGGGCGGATGCAGCA

Product: general secretion pathway protein J

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MRRARAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGHDQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPA
LLAGAGVLASESEGMRFVRGGVVNPLMRMPRSNLLTVGYRIHDGYLERLSWPLTDAAGSVKPTMQKLIPADSLHLQFYDG
TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS

Sequences:

>Translated_201_residues
MRRARAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGHDQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPA
LLAGAGVLASESEGMRFVRGGVVNPLMRMPRSNLLTVGYRIHDGYLERLSWPLTDAAGSVKPTMQKLIPADSLHLQFYDG
TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS
>Mature_201_residues
MRRARAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGHDQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPA
LLAGAGVLASESEGMRFVRGGVVNPLMRMPRSNLLTVGYRIHDGYLERLSWPLTDAAGSVKPTMQKLIPADSLHLQFYDG
TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG4795

COG function: function code U; Type II secretory pathway, component PulJ

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP J family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010055
- InterPro:   IPR012902
- InterPro:   IPR001120 [H]

Pfam domain/function: PF07963 N_methyl [H]

EC number: NA

Molecular weight: Translated: 22377; Mature: 22377

Theoretical pI: Translated: 10.70; Mature: 10.70

Prosite motif: PS00409 PROKAR_NTER_METHYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRARAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGHDQKLNLMQQTMSFLTH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGVVNPLMRMPRSNLLTVGYR
HHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHCCHHHHHHHCCCCCEEEEEEE
IHDGYLERLSWPLTDAAGSVKPTMQKLIPADSLHLQFYDGTRWQESWSSVQAIPVAVRMT
EHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHEEEEEE
LHSPQWGEIERIWLLRGPQLS
ECCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MRRARAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGHDQKLNLMQQTMSFLTH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGVVNPLMRMPRSNLLTVGYR
HHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHCCHHHHHHHCCCCCEEEEEEE
IHDGYLERLSWPLTDAAGSVKPTMQKLIPADSLHLQFYDGTRWQESWSSVQAIPVAVRMT
EHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHEEEEEE
LHSPQWGEIERIWLLRGPQLS
ECCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407845 [H]