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The map label for this gene is gspI2 [H]

Identifier: 157162433

GI number: 157162433

Start: 3142798

End: 3143169

Strand: Reverse

Name: gspI2 [H]

Synonym: EcHS_A3133

Alternate gene names: 157162433

Gene position: 3143169-3142798 (Counterclockwise)

Preceding gene: 157162434

Following gene: 157162432

Centisome position: 67.69

GC content: 59.41

Gene sequence:

>372_bases
ATGAAGCGCGGATTTACCTTGCTGGAAGTGATGCTCGCGCTGGCGATTTTTGCGCTGGCTGCCACGGCGGTGTTACAGAT
TGCCAGCGGTGCGCTGAGTAATCAGCAAATTCTTGAGGAAAAAACGGTAGCGGGCTGGGTGGCTGAAAACCAGACCGCGC
TGCTCTACCTGATGACCCGCGAGCAGCGGGCGGTCAGGCAGCAGGGCGAGAGCGATATGGCAGGAAGCCGCTGGTACTGG
CGAACCACACCGCTGAGTACAGGCAACGCGCTGCTCCAGGCGGTGGATATTGAAGTCAGCCTTCACGAAGACTTTTCGCC
GGTGATTCAGTCACGACGCGCCTGGTTTAGCGCCGTGGGAGGCCAACAGTGA

Upstream 100 bases:

>100_bases
TTACGCTGCGCTTCTACTCGGCGGCGCAAAACGCATGTTGGGCGGTAAAACTGGCGCACGATGGCGCGTTATCCCTCAAT
CAATGTGATGAGAGGATGCC

Downstream 100 bases:

>100_bases
GAAGGGCTCGCGCTGGTTTCACGTTACTGGAAATGCTGGTGGCAATTGCCATTTTTGCCTCGCTGGCACTGATGGCGCAG
CAGGTGACTAACGGCGTCAC

Product: general secretion pathway protein I

Products: NA

Alternate protein names: Cholera toxin secretion protein epsI [H]

Number of amino acids: Translated: 123; Mature: 123

Protein sequence:

>123_residues
MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQQILEEKTVAGWVAENQTALLYLMTREQRAVRQQGESDMAGSRWYW
RTTPLSTGNALLQAVDIEVSLHEDFSPVIQSRRAWFSAVGGQQ

Sequences:

>Translated_123_residues
MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQQILEEKTVAGWVAENQTALLYLMTREQRAVRQQGESDMAGSRWYW
RTTPLSTGNALLQAVDIEVSLHEDFSPVIQSRRAWFSAVGGQQ
>Mature_123_residues
MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQQILEEKTVAGWVAENQTALLYLMTREQRAVRQQGESDMAGSRWYW
RTTPLSTGNALLQAVDIEVSLHEDFSPVIQSRRAWFSAVGGQQ

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2165

COG function: function code NU; Type II secretory pathway, pseudopilin PulG

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP I family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010052
- InterPro:   IPR012902
- InterPro:   IPR001120
- InterPro:   IPR003413 [H]

Pfam domain/function: PF02501 GSPII_IJ; PF07963 N_methyl [H]

EC number: NA

Molecular weight: Translated: 13583; Mature: 13583

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00409 PROKAR_NTER_METHYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQQILEEKTVAGWVAENQTALLYLMTR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEC
EQRAVRQQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSLHEDFSPVIQSRRAWFSAVG
HHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHCEEEEECCCHHHHHHHHHHHHHHCC
GQQ
CCC
>Mature Secondary Structure
MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQQILEEKTVAGWVAENQTALLYLMTR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEC
EQRAVRQQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSLHEDFSPVIQSRRAWFSAVG
HHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHCEEEEECCCHHHHHHHHHHHHHHCC
GQQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]