Click here to switch to the map view.

The map label for this gene is gspK [H]

Identifier: 157162431

GI number: 157162431

Start: 3141222

End: 3142199

Strand: Reverse

Name: gspK [H]

Synonym: EcHS_A3131

Alternate gene names: 157162431

Gene position: 3142199-3141222 (Counterclockwise)

Preceding gene: 157162432

Following gene: 157162430

Centisome position: 67.67

GC content: 56.13

Gene sequence:

>978_bases
ATGATCACCTCACCACCAAAACGCGGAATGGCACTGGTCGTGGTGCTGGTATTGCTGGCAGTTATGATGCTGGTAACCAT
CACGCTTTCCGGGCGGATGCAGCAGCAACTTGGGCGAACGCGCAGCCAGCAGGAGTACCAGCAGGCGCTGTGGTACAGCG
CCAGTGCAGAAAGCCTGGCGCTGAGCGCGCTCAGTCTGAGCCTGAAAAATGAAAAGCGCGTGCATCTGGAACAGCCGTGG
GCTTCCGGCCCTCGTTTTTTCCCACTGCCGCAGGGGCAAATCGCCGTCACTCTGCGTGACGCACAGGCCTGCTTTAACCT
GAATGCCCTCGCTCAGCCCACAACGGCGTCGCGTCCGCTCGCGGTACAACAACTGATTGCCCTGATCACGCGCCTGGATG
TGCCTGCTTATCGGGCCGAACTGATAGCCGAAAGCCTGTGGGAGTTTATTGATGAAGACCGCAGCGTGCAGACGCGTCTG
GGCCGTGAAGACAGCGAATATCTCGCCCGCTCGGTGCCTTTCTACGCCGCCAATCAACCGCTGGCCGATATCAGCGAGAT
GCGCGTGGTGCAGGGAATGGACGCCGGGCTTTATCAAAAACTGAAACCGCTGGTCTGTGCGCTGCCGATGACCCGCCAGC
AAATCAACATCAATACTTTAGACGTCACGCAAAGTGTGATTCTTGAGGCGCTGTTTGACCCGTGGTTAAGCCCTGTTCAG
GCGCGGGCGTTATTACAACAACGTCCGGCGAAGGGCTGGGAAGATGTCGATCAGTTTCTTGCACAGCCGCTACTTGCTGA
CGTCGATGAGCGTACTAAAAAACAGCTAAAAACCGTCCTGAGCGTGGACAGCAATTACTTCTGGCTGCGTTCAGATATCA
CCGTGAATGAGATTGAACTGACGATGAACTCGTTAATTGTCCGCATGGGCCCACAACACTTTTCGGTTCTCTGGCATCAG
ACAGGAGAAAGTGAGTGA

Upstream 100 bases:

>100_bases
CGTCAGTACAGGCGATCCCTGTGGCAGTGCGCATGACTCTGCATTCGCCGCAATGGGGCGAGATTGAACGCATCTGGTTG
TTACGCGGGCCGCAATTATC

Downstream 100 bases:

>100_bases
GTTCCATCCTTGAGATTTTTTTCCCGCTTTGCGCCGCTGAGCCCATCCATTGGCAGCGCCGTACACCCGATGTGGAGCAC
GGTATCTGGTCTGACGTTGC

Product: general secretion pathway protein K

Products: NA

Alternate protein names: Cholera toxin secretion protein epsK [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLALSALSLSLKNEKRVHLEQPW
ASGPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTASRPLAVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRL
GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININTLDVTQSVILEALFDPWLSPVQ
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQLKTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQ
TGESE

Sequences:

>Translated_325_residues
MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLALSALSLSLKNEKRVHLEQPW
ASGPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTASRPLAVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRL
GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININTLDVTQSVILEALFDPWLSPVQ
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQLKTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQ
TGESE
>Mature_325_residues
MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLALSALSLSLKNEKRVHLEQPW
ASGPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTASRPLAVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRL
GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININTLDVTQSVILEALFDPWLSPVQ
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQLKTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQ
TGESE

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG3156

COG function: function code U; Type II secretory pathway, component PulK

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP K family [H]

Homologues:

Organism=Escherichia coli, GI1789729, Length=285, Percent_Identity=35.0877192982456, Blast_Score=126, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005628 [H]

Pfam domain/function: PF03934 GspK [H]

EC number: NA

Molecular weight: Translated: 36601; Mature: 36601

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHCCCHHHHH
LSALSLSLKNEKRVHLEQPWASGPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTASRPL
HHHHHHHHCCCCCEEECCCCCCCCEECCCCCCCEEEEEECHHHHHCCCCCCCCCCCCCCH
AVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRLGREDSEYLARSVPFYAANQP
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHCCCCCCCCCC
LADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININTLDVTQSVILEALFDPWLSPVQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHHH
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQLKTVLSVDSNYFWLRSDITVNEIEL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHH
TMNSLIVRMGPQHFSVLWHQTGESE
HHHHHHHHCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHCCCHHHHH
LSALSLSLKNEKRVHLEQPWASGPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTASRPL
HHHHHHHHCCCCCEEECCCCCCCCEECCCCCCCEEEEEECHHHHHCCCCCCCCCCCCCCH
AVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRLGREDSEYLARSVPFYAANQP
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHCCCCCCCCCC
LADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININTLDVTQSVILEALFDPWLSPVQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHHH
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQLKTVLSVDSNYFWLRSDITVNEIEL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHH
TMNSLIVRMGPQHFSVLWHQTGESE
HHHHHHHHCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]