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The map label for this gene is lsrK [H]

Identifier: 157160983

GI number: 157160983

Start: 1615439

End: 1617031

Strand: Reverse

Name: lsrK [H]

Synonym: EcHS_A1593

Alternate gene names: 157160983

Gene position: 1617031-1615439 (Counterclockwise)

Preceding gene: 157160984

Following gene: 157160982

Centisome position: 34.82

GC content: 52.17

Gene sequence:

>1593_bases
ATGGCTCGACTCTTTACCCCTTCAGAATCAAAGTACTACCTGATGGCGCTGGATGCAGGCACCGGAAGTATTCGGGCTGT
GATATTCGACCTGGAAGGCAATCAAATAGCAGTGGGACAGGCGGAGTGGCGGCATCTGGCAGTACCGGACGTTCCTGGTT
CTATGGAATTTGATCTCAACAAAAACTGGCAACTGGCGTGTGAGTGTATGCGCCAGGCGTTGCACAACGCCGGCATAGCC
CCGGAGTATATCGCTGCCGTTTCGGCATGTTCGATGCGTGAAGGCATTGTTTTATATAACAATGAAGGAACACCGATCTG
GGCCTGCGCCAATGTGGATGCCAGAGCGGCGCGCGAAGTTAGCGAACTTAAAGAACTGCACAACAATACCTTTGAAAACG
AAGTTTATCGCGCGACCGGACAAACACTGGCTTTAAGTGCCATCCCCAGATTACTTTGGCTGGCGCACCATCGTTCCGAT
ATTTACCGTCAGGCATCAACCATCACCATGATCAGCGACTGGCTGGCCTATATGCTCAGCGGCGAACTTGCGGTGGATCC
CTCTAACGCTGGCACCACGGGACTTCTTGATCTAACCACCCGTGACTGGAAACCTGCATTGCTGGATATGGCTGGCCTGC
GTGCCGATATTCTTTCTCCTGTCAAAGAAACCGGCACATTGCTGGGCGTGGTAAGTTCACAAGCGGCGGAACTCTGCGGT
CTGAAGGCGGGCACTCCTGTGGTCGTTGGAGGAGGCGACGTGCAGCTTGGTTGCCTGGGGTTAGGCGTTGTGCGTCCGGC
ACAAACCGCGGTTCTTGGCGGCACATTCTGGCAGCAAGTTGTAAACTTAGCCGCGCCGGTGACAGACCCAGAAATGAACG
TGCGCGTTAATCCCCATGTAATTCCTGGCATGGTACAAGCTGAATCTATAAGCTTTTTTACCGGACTCACCATGCGCTGG
TTCCGCGATGCTTTCTGTGCCGAAGAAAAATTGATTGCCGAACGTTTAGGCATCGACACCTATACGCTGCTGGAAGAGAT
GACCAGTCGGGTGCCGCCTGGGTCGTGGGGCGTAATGCCGATCTTCTCCGACAGAATGCGCTTTAAAACCTGGTATCACG
CTGCGCCTTCCTTTATTAATTTGTCCATTGACCCGGATAAATGTAACAAAGCGACATTGTTCCGTGCGCTGGAAGAAAAT
GCGGCGATTGTTTCAGCGTGTAACTTGCAGCAAATTGCTGATTTTTCGAATATTCATCCTTCATCGTTAGTCTTTGCAGG
CGGAGGTTCAAAAGGGAAATTATGGAGTCAAATTCTCGCTGATGTCTCGGGATTACCCGTCAATATTCCGGTGGTCAAAG
AAGCCACTGCATTAGGATGTGCCATTGCAGCTGGCGTCGGTGCCGGAATTTTTTCATCAATGGCAGAAACCGGAGAACGG
CTGGTTCGCTGGGAACGGACGCACACACCAGCCCCGGAAAAGCATGAACTTTATCAGGATTCACGCGATAAGTGGCAGGC
AGTTTATCAGGATCAGCTGGGGCTGGTTGATCATGGACTGACGACGTCGTTATGGAAAGCGCCTGGGTTATAG

Upstream 100 bases:

>100_bases
AGCGGCGATTTTACGTAGTTAAATTTGCATGACCTTATTACCGGGAATGATGGAGAACGTATAGAGGAACAGACAGAACG
CATAAGCCGAGGATAATCTA

Downstream 100 bases:

>100_bases
TTTCATTGACAGCCACATCATGATGACCGTGGCTGTTAGTTTTTGAGTATAGTTATTCTTATTTCCCTTCTTTTAGAACT
GGCTTGTTAAATGCCAGCTT

Product: autoinducer-2 (AI-2) kinase

Products: NA

Alternate protein names: AI-2 kinase [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWRHLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIA
PEYIAAVSACSMREGIVLYNNEGTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADILSPVKETGTLLGVVSSQAAELCG
LKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTFWQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRW
FRDAFCAEEKLIAERLGIDTYTLLEEMTSRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKATLFRALEEN
AAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIPVVKEATALGCAIAAGVGAGIFSSMAETGER
LVRWERTHTPAPEKHELYQDSRDKWQAVYQDQLGLVDHGLTTSLWKAPGL

Sequences:

>Translated_530_residues
MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWRHLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIA
PEYIAAVSACSMREGIVLYNNEGTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADILSPVKETGTLLGVVSSQAAELCG
LKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTFWQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRW
FRDAFCAEEKLIAERLGIDTYTLLEEMTSRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKATLFRALEEN
AAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIPVVKEATALGCAIAAGVGAGIFSSMAETGER
LVRWERTHTPAPEKHELYQDSRDKWQAVYQDQLGLVDHGLTTSLWKAPGL
>Mature_529_residues
ARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWRHLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAP
EYIAAVSACSMREGIVLYNNEGTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSDI
YRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADILSPVKETGTLLGVVSSQAAELCGL
KAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTFWQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWF
RDAFCAEEKLIAERLGIDTYTLLEEMTSRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKATLFRALEENA
AIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIPVVKEATALGCAIAAGVGAGIFSSMAETGERL
VRWERTHTPAPEKHELYQDSRDKWQAVYQDQLGLVDHGLTTSLWKAPGL

Specific function: Catalyzes the phosphorylation of autoinducer 2 (AI-2) to phospho-AI-2, which subsequently inactivates the transcriptional regulator LsrR and leads to the transcription of the lsr operon. Phosphorylates the ring-open form of 4,5-dihydroxy-2,3- pentanedione

COG id: COG1070

COG function: function code G; Sugar (pentulose and hexulose) kinases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787789, Length=530, Percent_Identity=99.2452830188679, Blast_Score=1084, Evalue=0.0,
Organism=Escherichia coli, GI1790005, Length=505, Percent_Identity=25.1485148514851, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1790361, Length=501, Percent_Identity=26.3473053892216, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1789138, Length=508, Percent_Identity=24.0157480314961, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1789168, Length=505, Percent_Identity=23.1683168316832, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789987, Length=510, Percent_Identity=25.0980392156863, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17535599, Length=274, Percent_Identity=26.2773722627737, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018484 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: NA

Molecular weight: Translated: 57545; Mature: 57414

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWRHLAVPDVPGSMEFDLN
CCCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCEEEECCCCCCEEECCCCCCCEEECCC
KNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNEGTPIWACANVDARAAREV
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEECCCCCEEEEECCCCHHHHHHH
SELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSDIYRQASTITMISDWLAYMLS
HHHHHHHCCCHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADILSPVKETGTLLGVVSSQAAELCG
CCEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHC
LKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTFWQQVVNLAAPVTDPEMNVRVNPHV
CCCCCEEEECCCCEEEEEEEECCCCCCHHHHCCHHHHHHHHHHHCCCCCCCCEEEECCCC
IPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAERLGIDTYTLLEEMTSRVPPGSWGVMP
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCH
IFSDRMRFKTWYHAAPSFINLSIDPDKCNKATLFRALEENAAIVSACNLQQIADFSNIHP
HHHCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHCCCCC
SSLVFAGGGSKGKLWSQILADVSGLPVNIPVVKEATALGCAIAAGVGAGIFSSMAETGER
CCEEEECCCCCCHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVRWERTHTPAPEKHELYQDSRDKWQAVYQDQLGLVDHGLTTSLWKAPGL
HHHHHCCCCCCCCHHHHHHCHHHHHHHHHHHCCCCCCCCCCHHHHCCCCC
>Mature Secondary Structure 
ARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWRHLAVPDVPGSMEFDLN
CCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCEEEECCCCCCEEECCCCCCCEEECCC
KNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNEGTPIWACANVDARAAREV
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEECCCCCEEEEECCCCHHHHHHH
SELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSDIYRQASTITMISDWLAYMLS
HHHHHHHCCCHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADILSPVKETGTLLGVVSSQAAELCG
CCEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHC
LKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTFWQQVVNLAAPVTDPEMNVRVNPHV
CCCCCEEEECCCCEEEEEEEECCCCCCHHHHCCHHHHHHHHHHHCCCCCCCCEEEECCCC
IPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAERLGIDTYTLLEEMTSRVPPGSWGVMP
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCH
IFSDRMRFKTWYHAAPSFINLSIDPDKCNKATLFRALEENAAIVSACNLQQIADFSNIHP
HHHCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHCCCCC
SSLVFAGGGSKGKLWSQILADVSGLPVNIPVVKEATALGCAIAAGVGAGIFSSMAETGER
CCEEEECCCCCCHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVRWERTHTPAPEKHELYQDSRDKWQAVYQDQLGLVDHGLTTSLWKAPGL
HHHHHCCCCCCCCHHHHHHCHHHHHHHHHHHCCCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA