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The map label for this gene is paaK [H]

Identifier: 157160874

GI number: 157160874

Start: 1479623

End: 1480693

Strand: Direct

Name: paaK [H]

Synonym: EcHS_A1479

Alternate gene names: 157160874

Gene position: 1479623-1480693 (Clockwise)

Preceding gene: 157160873

Following gene: 157160875

Centisome position: 31.86

GC content: 53.41

Gene sequence:

>1071_bases
ATGACAACGTTTCATTCCTTAACGGTGGCAAAAGTGGAGCCGGAAACCCGTGATGCGGTGACCATTACCTTTGCGGTGCC
CCAGCCTTTGCAGGAGGCGTATCGCTTTCGCCCCGGTCAACATTTGACCTTAAAAGCCAGCTTTGATGGTGAAGAATTAC
GCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTC
TCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCC
GCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCA
CTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCA
CTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCT
GCTTAACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCAT
TTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCTTAAAAGCACTGGGAATGCCAGATAAAACCATTCAT
CTGGAGCGGTTTAATACGCCTGGCACGCGCGTCAAACGTAGCGTTAACGTGCAAAGTGACGGACAAAAAGTGACTGTACG
TCAGGATGGGCGGGATCGGGAAATCGTGCTTAATGCCGACGATGAAAGCATTCTCGATGCGGCATTGCGCCAGGGGGCGG
ATCTGCCCTATGCCTGCAAAGGCGGCGTCTGTGCGACCTGCAAATGCAAAGTGCTGCGTGGCAAAGTGGCGATGGAAACC
AATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGT
GGTTGACTTTGACGCGAAGGGGATGGCATGA

Upstream 100 bases:

>100_bases
ACACTTATCAGTGAATTTGGTTCCACGGCCTGCAAAGCATTGTATCGCTGCGATAGTTGCCGCGAACCTTTCGATTATTT
CAAATGTATTTGAGGATGCC

Downstream 100 bases:

>100_bases
GCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAAT
GCCCTGCTGATGCAACTGGT

Product: phenylacetate-CoA oxygenase/reductase, PaaK subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTLKASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRF
SRYAREHIRQGMTLEVMVPQGHFGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
LADLKDKYPQRLQLLCIFSQETLDSDLLNGRIDGEKLQSLGASLINFRLYDEAFICGPAAMMDDAETALKALGMPDKTIH
LERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIVLNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMET
NYSLEPDELAAGYVLSCQALPLTSDVVVDFDAKGMA

Sequences:

>Translated_356_residues
MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTLKASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRF
SRYAREHIRQGMTLEVMVPQGHFGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
LADLKDKYPQRLQLLCIFSQETLDSDLLNGRIDGEKLQSLGASLINFRLYDEAFICGPAAMMDDAETALKALGMPDKTIH
LERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIVLNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMET
NYSLEPDELAAGYVLSCQALPLTSDVVVDFDAKGMA
>Mature_355_residues
TTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTLKASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRFS
RYAREHIRQGMTLEVMVPQGHFGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQAL
ADLKDKYPQRLQLLCIFSQETLDSDLLNGRIDGEKLQSLGASLINFRLYDEAFICGPAAMMDDAETALKALGMPDKTIHL
ERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIVLNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETN
YSLEPDELAAGYVLSCQALPLTSDVVVDFDAKGMA

Specific function: May be part of a multicomponent oxygenase involved in phenylacetyl-CoA hydroxylation [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1787658, Length=356, Percent_Identity=99.438202247191, Blast_Score=731, Evalue=0.0,
Organism=Escherichia coli, GI1787098, Length=338, Percent_Identity=28.1065088757396, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788104, Length=351, Percent_Identity=23.0769230769231, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788903, Length=216, Percent_Identity=27.7777777777778, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17563926, Length=212, Percent_Identity=26.8867924528302, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323510, Length=201, Percent_Identity=27.8606965174129, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR011884
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39307; Mature: 39176

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTLKASFDGEELRRCYSIC
CCCEEEEEEEEECCCCCCEEEEEEECCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHH
RSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGHFGYQPQAERQGRYLAIAA
HHHCCCCEEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHCCCEEEEEE
GSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQALADLKDKYPQRLQLLCIFSQ
CCCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECH
ETLDSDLLNGRIDGEKLQSLGASLINFRLYDEAFICGPAAMMDDAETALKALGMPDKTIH
HHHCHHHHCCCCCHHHHHHHHHHHHEEEEECCEEEECCHHHHCCHHHHHHHCCCCCCEEE
LERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIVLNADDESILDAALRQGADLPYACK
EEECCCCCHHHEEEECCCCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHCCCCCCEECC
GGVCATCKCKVLRGKVAMETNYSLEPDELAAGYVLSCQALPLTSDVVVDFDAKGMA
CCEEEEEEEEEEECEEEEECCCCCCHHHHCCCEEEEEEECCCCCCEEEEECCCCCC
>Mature Secondary Structure 
TTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTLKASFDGEELRRCYSIC
CCEEEEEEEEECCCCCCEEEEEEECCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHH
RSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGHFGYQPQAERQGRYLAIAA
HHHCCCCEEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHCCCEEEEEE
GSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQALADLKDKYPQRLQLLCIFSQ
CCCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECH
ETLDSDLLNGRIDGEKLQSLGASLINFRLYDEAFICGPAAMMDDAETALKALGMPDKTIH
HHHCHHHHCCCCCHHHHHHHHHHHHEEEEECCEEEECCHHHHCCHHHHHHHCCCCCCEEE
LERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIVLNADDESILDAALRQGADLPYACK
EEECCCCCHHHEEEECCCCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHCCCCCCEECC
GGVCATCKCKVLRGKVAMETNYSLEPDELAAGYVLSCQALPLTSDVVVDFDAKGMA
CCEEEEEEEEEEECEEEEECCCCCCHHHHCCCEEEEEEECCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]