BacMap

Definition	Escherichia coli HS, complete genome.
Accession	NC_009800
Length	4,643,538

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The map label for this gene is paaF

Identifier: 157160875

GI number: 157160875

Start: 1480690

End: 1481457

Strand: Direct

Name: paaF

Synonym: EcHS_A1480

Alternate gene names: 157160875

Gene position: 1480690-1481457 (Clockwise)

Preceding gene: 157160874

Following gene: 157160876

Centisome position: 31.89

GC content: 54.69

Gene sequence:

>768_bases
ATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAA
TAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCG
GTAATGCACGCTTTTTTGCCGCCGGGGCCGATCTCAACGAAATGGCAGAAAAAGATCTCGCGGCCACCTTAAACGATACA
CGTCCGCAGCTATGGGCGCGATTGCAGGCCTTTAATAAACCACTTATCGCAGCCGTCAATGGTTACGCGCTTGGGGCGGG
TTGCGAACTGGCATTGTTGTGCGATGTGGTGGTTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCA
TCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGA
GAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGC
CTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGG
AAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGC
ATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAA

Upstream 100 bases:

>100_bases
GTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGAC
TTTGACGCGAAGGGGATGGC

Downstream 100 bases:

>100_bases
TGATGGAATTCATCCTCAGTCATGTAGAAAAGGGCGTGATGACACTAACGCTCAACCGCCCGGAACGCCTGAACAGTTTT
AATGATGAGATGCACGCACA

Product: enoyl-CoA hydratase-isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATDTSISVCVITGNARFFAAGADLNEMAEKDLAATLNDT
RPQLWARLQAFNKPLIAAVNGYALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQAGLAQERQLFTLLAATEDRHEG
ISAFLQKRTPDFKGR

Sequences:

>Translated_255_residues
MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATDTSISVCVITGNARFFAAGADLNEMAEKDLAATLNDT
RPQLWARLQAFNKPLIAAVNGYALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQAGLAQERQLFTLLAATEDRHEG
ISAFLQKRTPDFKGR
>Mature_254_residues
SELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATDTSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTR
PQLWARLQAFNKPLIAAVNGYALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSGE
SITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQAGLAQERQLFTLLAATEDRHEGI
SAFLQKRTPDFKGR

Specific function: Could possibly oxidize fatty acids using specific components

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family

Homologues:

Organism=Homo sapiens, GI194097323, Length=246, Percent_Identity=45.5284552845528, Blast_Score=222, Evalue=3e-58,
Organism=Homo sapiens, GI4502327, Length=258, Percent_Identity=36.046511627907, Blast_Score=131, Evalue=7e-31,
Organism=Homo sapiens, GI31542718, Length=239, Percent_Identity=31.3807531380753, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI70995211, Length=232, Percent_Identity=32.7586206896552, Blast_Score=111, Evalue=6e-25,
Organism=Homo sapiens, GI150378541, Length=254, Percent_Identity=30.3149606299213, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI260274832, Length=256, Percent_Identity=27.34375, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI45643119, Length=256, Percent_Identity=27.34375, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI260275230, Length=256, Percent_Identity=27.34375, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI68989263, Length=253, Percent_Identity=31.6205533596838, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI20127408, Length=203, Percent_Identity=33.9901477832512, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI25453481, Length=259, Percent_Identity=26.6409266409266, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI51538573, Length=259, Percent_Identity=26.6409266409266, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI4757966, Length=253, Percent_Identity=28.8537549407115, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI51538581, Length=253, Percent_Identity=28.8537549407115, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI221136756, Length=264, Percent_Identity=28.4090909090909, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI221136753, Length=264, Percent_Identity=28.4090909090909, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI221307494, Length=264, Percent_Identity=28.4090909090909, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI4757968, Length=258, Percent_Identity=25.5813953488372, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI49355787, Length=258, Percent_Identity=25.5813953488372, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI116235487, Length=258, Percent_Identity=27.1317829457364, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI213417737, Length=223, Percent_Identity=28.6995515695067, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI157694516, Length=223, Percent_Identity=28.6995515695067, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI37594469, Length=185, Percent_Identity=26.4864864864865, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI62530384, Length=229, Percent_Identity=27.0742358078603, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI37594471, Length=185, Percent_Identity=26.4864864864865, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI261878539, Length=163, Percent_Identity=31.9018404907975, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI157694520, Length=207, Percent_Identity=27.536231884058, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=100, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI221142681, Length=264, Percent_Identity=36.3636363636364, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=35.8778625954198, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1788597, Length=247, Percent_Identity=31.9838056680162, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1788682, Length=150, Percent_Identity=41.3333333333333, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082183, Length=237, Percent_Identity=27.4261603375527, Blast_Score=100, Evalue=6e-23,
Organism=Escherichia coli, GI1790281, Length=179, Percent_Identity=34.0782122905028, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=47.6, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=47.7366255144033, Blast_Score=226, Evalue=6e-60,
Organism=Caenorhabditis elegans, GI17540714, Length=230, Percent_Identity=30.8695652173913, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17560910, Length=217, Percent_Identity=29.9539170506912, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17549921, Length=212, Percent_Identity=31.1320754716981, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17535521, Length=254, Percent_Identity=29.1338582677165, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17558304, Length=208, Percent_Identity=32.6923076923077, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17540306, Length=233, Percent_Identity=29.6137339055794, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=238, Percent_Identity=28.9915966386555, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=232, Percent_Identity=25.8620689655172, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17508953, Length=179, Percent_Identity=33.5195530726257, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI25144276, Length=180, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17508951, Length=180, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI25144160, Length=267, Percent_Identity=25.8426966292135, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25144157, Length=267, Percent_Identity=25.8426966292135, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=191, Percent_Identity=28.2722513089005, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=45.9677419354839, Blast_Score=225, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=45.9677419354839, Blast_Score=225, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24653139, Length=265, Percent_Identity=33.9622641509434, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI21357171, Length=253, Percent_Identity=29.6442687747036, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19920382, Length=259, Percent_Identity=30.1158301158301, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24654903, Length=212, Percent_Identity=28.7735849056604, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19922422, Length=255, Percent_Identity=24.7058823529412, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24650670, Length=257, Percent_Identity=26.4591439688716, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24583165, Length=248, Percent_Identity=25.4032258064516, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921018, Length=235, Percent_Identity=26.3829787234043, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19921000, Length=174, Percent_Identity=33.9080459770115, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24583077, Length=174, Percent_Identity=33.9080459770115, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24583079, Length=174, Percent_Identity=33.9080459770115, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PAAF_ECOLI (P76082)

Other databases:

- EMBL:   X97452
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   D64890
- RefSeq:   AP_002020.1
- RefSeq:   NP_415911.1
- ProteinModelPortal:   P76082
- SMR:   P76082
- DIP:   DIP-10425N
- STRING:   P76082
- EnsemblBacteria:   EBESCT00000004811
- EnsemblBacteria:   EBESCT00000016921
- GeneID:   946011
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1388
- KEGG:   eco:b1393
- EchoBASE:   EB3503
- EcoGene:   EG13740
- eggNOG:   COG1024
- GeneTree:   EBGT00050000009097
- HOGENOM:   HBG748731
- OMA:   WGRIDAF
- ProtClustDB:   PRK09674
- BioCyc:   EcoCyc:G6714-MONOMER
- BioCyc:   MetaCyc:G6714-MONOMER
- Genevestigator:   P76082
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376
- Gene3D:   G3DSA:1.10.12.10

Pfam domain/function: PF00378 ECH

EC number: =4.2.1.17

Molecular weight: Translated: 27238; Mature: 27107

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATDTSISVCVITGNARFFA
CCCHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEE
AGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGYALGAGCELALLCDVVVAG
ECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCEEECCCCHHHHHHHHHHCC
ENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSGESITAQQAQQAGLVSDVFPS
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHCCCC
DLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQAGLAQERQLFTLLAATEDRHEG
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHH
ISAFLQKRTPDFKGR
HHHHHHHCCCCCCCC
>Mature Secondary Structure 
SELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATDTSISVCVITGNARFFA
CCHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEE
AGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGYALGAGCELALLCDVVVAG
ECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCEEECCCCHHHHHHHHHHCC
ENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSGESITAQQAQQAGLVSDVFPS
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHCCCC
DLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQAGLAQERQLFTLLAATEDRHEG
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHH
ISAFLQKRTPDFKGR
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858