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The map label for this gene is 154248124

Identifier: 154248124

GI number: 154248124

Start: 4654216

End: 4655586

Strand: Direct

Name: 154248124

Synonym: Xaut_4204

Alternate gene names: NA

Gene position: 4654216-4655586 (Clockwise)

Preceding gene: 154248123

Following gene: 154248125

Centisome position: 87.67

GC content: 67.98

Gene sequence:

>1371_bases
ATGGCCCGGGACACCCTTGACGCGACCCCCATTGCCGGCCGGCACGAGCTGGTGGAGTGGATGGAACAGGGGTCCAAACC
GGAGCGGGCCTTTCGCATCGGTACCGAGCACGAGAAGATCCCCTTCACCCTCGGCCGCCACGAACCGGTGCCCTACGAGG
GGCCCAAGGGCATCCGCAAGCTCCTGGAGGGCATGCGCTCCCTGCTCGGCTGGGAGCCCATCATGGAAGGCCCCACCATC
ATCGGCCTCGCCGATGTGACCGGGGGCGGCGCCATCTCGCTGGAGCCGGGCGGACAGTTCGAGCTGTCGGGCGCGCCGGT
CTCCACCATCCACGAGACCTGCGCGGAGCTGAATGCGCATCTGGCGCAGGTGCGCGAAGTTGCGGAGCCGCTGGGCATCG
GCTTCCTCGGCATCGGCATGAGCCCCAAGTGGACCCGCACCGAGACCCCGGTGATGCCCAAGGGCCGTTACAAGATCATG
GCCGGCTACATGCCTAAGGTGGGCAAGCTGGGCCTCGACATGATGTTCCGCACCTGCACCGTGCAGGTGAATCTCGACTT
CTCGTCCGAAGCCGACATGGTGCGGAAGATGCGCGTCGGCCTCGCGCTCCAGCCGGTGGCGACCGCCCTGTTCGCCAATT
CGCCGTTCACCGAAGGCAAGCCCAACGGCTTCCTCTCGTTCCGCTCCGAGATCTGGCGCGACACCGACAATGCCCGCTCG
GGCATGCTGCCCTTCGCCTTCGAGGACGGCTTCGGCTTCGATCGTTACGTGGACTACGCCCTCGACGTGCCCATGTATTT
CGTGAAGCGCGGCGAGAGCTACGTGGACGTGTCGGGCACCTCGTTCCGCGACCTGCTCGCCGGCCGCCATCCGCTGCTGC
CGGGCGAGACCGCCACCCTCTCGGACTGGATCAACCACCTCTCCACCATCTTCCCCGAGGTACGCCTGAAGCGCTTTTTG
GAGATGCGCGGCGCCGATGCCGGCCCCTGGGCCAAGCTGTGCGCGCTGCCCGCGCTCTGGGTGGGCCTGCTCTACGACCA
GCAAAGCCTCGATGCCGCCTGGGACCTCGCCAAGGACTGGACCGCCGAGCAGCGCCAGCAGCTGCGGGACGACGTGCCCC
GCCTTGGCTTGAAGGCCGAGATCGCCGGCCGCCAGCTGCGCGATATCGCCCGCGACGTGCTCAAGCTGTCGGAAGCCGGG
CTGAAGCGGCGCGGCTTCCACGACCGGCTGGGCCGCGACGAGACGCGCTTCCTGGAGCCGCTGGAAGAGATCGTGCTCAC
CGGCCGCTCCCCCGCCGACAAGCTGCTGGCGCTCTACAACGGCGAATGGGACCACTCGGTGGAACCGGCCTTCCAGGCGC
TGGCTTACTGA

Upstream 100 bases:

>100_bases
TGAAGGGTGCGTCCGCGCGGTATTGAGAAACCGAAGCGTCTGTTGCCTTGGCCGGGCCGCCGTGTATGGTCTCGCGCCCA
TTTTGTTGAAGAGATTTCGT

Downstream 100 bases:

>100_bases
GTCGGGGTGGGGCGGTCAGCAACTCCGCCCGCAGTTAACGCATGATCTAGGCGCATTGCAGCCATGCGCTGATGCCATGC
GACGAAGTCCGGTTTCCACC

Product: glutamate--cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate/Cysteine Ligase Family Protein; Glutamate-Cysteine Ligase GshA; Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase Protein; Glutathione Synthetase; Glutamate-Cysteine Ligase Gsha; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family Protein; Glutamate Cystein Ligase Protein; Glutamate-Cysteine Ligase GSHA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MARDTLDATPIAGRHELVEWMEQGSKPERAFRIGTEHEKIPFTLGRHEPVPYEGPKGIRKLLEGMRSLLGWEPIMEGPTI
IGLADVTGGGAISLEPGGQFELSGAPVSTIHETCAELNAHLAQVREVAEPLGIGFLGIGMSPKWTRTETPVMPKGRYKIM
AGYMPKVGKLGLDMMFRTCTVQVNLDFSSEADMVRKMRVGLALQPVATALFANSPFTEGKPNGFLSFRSEIWRDTDNARS
GMLPFAFEDGFGFDRYVDYALDVPMYFVKRGESYVDVSGTSFRDLLAGRHPLLPGETATLSDWINHLSTIFPEVRLKRFL
EMRGADAGPWAKLCALPALWVGLLYDQQSLDAAWDLAKDWTAEQRQQLRDDVPRLGLKAEIAGRQLRDIARDVLKLSEAG
LKRRGFHDRLGRDETRFLEPLEEIVLTGRSPADKLLALYNGEWDHSVEPAFQALAY

Sequences:

>Translated_456_residues
MARDTLDATPIAGRHELVEWMEQGSKPERAFRIGTEHEKIPFTLGRHEPVPYEGPKGIRKLLEGMRSLLGWEPIMEGPTI
IGLADVTGGGAISLEPGGQFELSGAPVSTIHETCAELNAHLAQVREVAEPLGIGFLGIGMSPKWTRTETPVMPKGRYKIM
AGYMPKVGKLGLDMMFRTCTVQVNLDFSSEADMVRKMRVGLALQPVATALFANSPFTEGKPNGFLSFRSEIWRDTDNARS
GMLPFAFEDGFGFDRYVDYALDVPMYFVKRGESYVDVSGTSFRDLLAGRHPLLPGETATLSDWINHLSTIFPEVRLKRFL
EMRGADAGPWAKLCALPALWVGLLYDQQSLDAAWDLAKDWTAEQRQQLRDDVPRLGLKAEIAGRQLRDIARDVLKLSEAG
LKRRGFHDRLGRDETRFLEPLEEIVLTGRSPADKLLALYNGEWDHSVEPAFQALAY
>Mature_455_residues
ARDTLDATPIAGRHELVEWMEQGSKPERAFRIGTEHEKIPFTLGRHEPVPYEGPKGIRKLLEGMRSLLGWEPIMEGPTII
GLADVTGGGAISLEPGGQFELSGAPVSTIHETCAELNAHLAQVREVAEPLGIGFLGIGMSPKWTRTETPVMPKGRYKIMA
GYMPKVGKLGLDMMFRTCTVQVNLDFSSEADMVRKMRVGLALQPVATALFANSPFTEGKPNGFLSFRSEIWRDTDNARSG
MLPFAFEDGFGFDRYVDYALDVPMYFVKRGESYVDVSGTSFRDLLAGRHPLLPGETATLSDWINHLSTIFPEVRLKRFLE
MRGADAGPWAKLCALPALWVGLLYDQQSLDAAWDLAKDWTAEQRQQLRDDVPRLGLKAEIAGRQLRDIARDVLKLSEAGL
KRRGFHDRLGRDETRFLEPLEEIVLTGRSPADKLLALYNGEWDHSVEPAFQALAY

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 50832; Mature: 50701

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDTLDATPIAGRHELVEWMEQGSKPERAFRIGTEHEKIPFTLGRHEPVPYEGPKGIRK
CCCCCCCCCCCCCHHHHHHHHHCCCCCCHHEECCCCCCCCCEECCCCCCCCCCCCHHHHH
LLEGMRSLLGWEPIMEGPTIIGLADVTGGGAISLEPGGQFELSGAPVSTIHETCAELNAH
HHHHHHHHHCCCHHHCCCCEEEEEECCCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHH
LAQVREVAEPLGIGFLGIGMSPKWTRTETPVMPKGRYKIMAGYMPKVGKLGLDMMFRTCT
HHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHEEEEE
VQVNLDFSSEADMVRKMRVGLALQPVATALFANSPFTEGKPNGFLSFRSEIWRDTDNARS
EEEEECCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
GMLPFAFEDGFGFDRYVDYALDVPMYFVKRGESYVDVSGTSFRDLLAGRHPLLPGETATL
CCCCEEECCCCCHHHHHHHHHCCHHHHHHCCCCEEECCCCHHHHHHCCCCCCCCCCCHHH
SDWINHLSTIFPEVRLKRFLEMRGADAGPWAKLCALPALWVGLLYDQQSLDAAWDLAKDW
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHC
TAEQRQQLRDDVPRLGLKAEIAGRQLRDIARDVLKLSEAGLKRRGFHDRLGRDETRFLEP
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHH
LEEIVLTGRSPADKLLALYNGEWDHSVEPAFQALAY
HHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHCC
>Mature Secondary Structure 
ARDTLDATPIAGRHELVEWMEQGSKPERAFRIGTEHEKIPFTLGRHEPVPYEGPKGIRK
CCCCCCCCCCCCHHHHHHHHHCCCCCCHHEECCCCCCCCCEECCCCCCCCCCCCHHHHH
LLEGMRSLLGWEPIMEGPTIIGLADVTGGGAISLEPGGQFELSGAPVSTIHETCAELNAH
HHHHHHHHHCCCHHHCCCCEEEEEECCCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHH
LAQVREVAEPLGIGFLGIGMSPKWTRTETPVMPKGRYKIMAGYMPKVGKLGLDMMFRTCT
HHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHEEEEE
VQVNLDFSSEADMVRKMRVGLALQPVATALFANSPFTEGKPNGFLSFRSEIWRDTDNARS
EEEEECCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
GMLPFAFEDGFGFDRYVDYALDVPMYFVKRGESYVDVSGTSFRDLLAGRHPLLPGETATL
CCCCEEECCCCCHHHHHHHHHCCHHHHHHCCCCEEECCCCHHHHHHCCCCCCCCCCCHHH
SDWINHLSTIFPEVRLKRFLEMRGADAGPWAKLCALPALWVGLLYDQQSLDAAWDLAKDW
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHC
TAEQRQQLRDDVPRLGLKAEIAGRQLRDIARDVLKLSEAGLKRRGFHDRLGRDETRFLEP
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHH
LEEIVLTGRSPADKLLALYNGEWDHSVEPAFQALAY
HHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA