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The map label for this gene is kstD [H]

Identifier: 154246642

GI number: 154246642

Start: 2991274

End: 2992920

Strand: Reverse

Name: kstD [H]

Synonym: Xaut_2702

Alternate gene names: 154246642

Gene position: 2992920-2991274 (Counterclockwise)

Preceding gene: 154246643

Following gene: 154246641

Centisome position: 56.38

GC content: 69.64

Gene sequence:

>1647_bases
ATGGCGACGGCGCTGTTCGCCGCCATCGCCGGCCTGAAGGTGCTGCTGGTGGAGCGCACCGAATATGTCGGCGGCACCAC
CGCCTTGTCCGCTGCCACCACCTGGATCCCCATGAACCGGCACTCCTCCGAGGTGGCGGTGGATGACAGTCTCGATAAGG
CCGAGCACTTCCTGGATGGCGTGGTGGGCAACCGTTCCCCGGCGGTGCTGCGGCGCGCCTTCCTGGAGAGCGGGCCGCAG
GCCATCGCCACCCTCGAAGACCAGTCGCAGGTGCGCTTTCGCCCCTACGCCACCCATCCCGACTACGAGCAGGACCATGA
GGGCGCCACCCTGCGCGGGCGGGCGCTGGAGCCGGTGCCCTTCGACGGTCGCAGCCTCGGCGCAGACCTCGCGCGCATCC
GGCCGCCGATCCCGGAATTCACCATCTTCGGCGGCATGATGATCGACCGCACCGACATCGGGCACCTGCTGGGACTGACG
AAGTCGTGGCGCTCCTTCCGGCATGCCGCCTCGATCCTCGCCTGGTACGGCCGCGACCGGCTGGCGCAGCGGCGCGGCAG
CCGGCTGGTGATGGGAAACGCCCTGATCGGGCGCATGCTCGCATCCCTGAAGGCGCGCAACGTCCCGATCCTGCTGGAGG
CCACCGTCCGCGCCGTCGACGCGGGGCGGGGCGGCGTGGAAGGCGTCACCGTGGCGCAGAACGGCGCGGAGCGGCAGATC
CGCGTCCGTCGTGGCCTCGTGATCGCGGCGGGCGGCTTCAACCGCCACCCCACCCGCCGTGCCGAGCTTCTGCCCAAGCC
GACACCCGCCTACAGCCCGGCCGCGCCCGGGCATACCGGCGCCATGCAGGACCTCGCCCTGAAGCTCGGCGCCCGCTTCG
GCACGGGGGGCGACCAGCCGGCCTTCTGGGCGCCGGTCTCGGTGCGCCGCCGCGCCGACGGTTCCACCGCGGTCTTCCCC
CATTTCGTGATGGACCGCTCCAAGCCAGGCACCGTGTGCGTGGACCAGACCGGGCGGCGCTTCGTGAACGAATCCACCTC
CTATCACCAGTTCGGCAAGGCCATGTTCGCGGCGAACCGGGATCGGCCGACCATCCCCTGCTTCATCATCACCGATGCGG
TGGGCCTGAAGAGATTTGGCCTCGGCATGGTGCGCATGGGCACGCGCAACCTGAAGCCGTTCCTCGACGACGGCTATCTG
GTGGAAGCGCCCACCATCGCCGCCCTCGCTGCGAAGCTGAACATGGATGCCGCCGTATTGGAAGAAACAGTCGGCGCCAT
GAACACCTTTGCCAAGACCGGTGTCGATACGCAGTTCGGTCGCGGCACCACCGCCTATCACCGGATCAACGGCGATGCGG
CGCACGGTCCCAATCCCACCCTCGGCCCGATCGATACCGCGCCCTTCTACGCCGTGCGGCTCTATCCCGGCGACATCGGT
GCAGCCACCGGCCTCGTCATCGACGAATGGGCGCGGGTGCTGAAGGAGGACGGCAGCCCGGTTCCCGGCCTCTATGCCTG
CGGCAACGAGGCCCAGTCCATCATGGGCGGGACCTATCCCGGCCCGGGCATCACCATCGGCCCGGCCATCACCTTCGCCT
ATCGCGCCGTGCGCCATGCCTGCCAAGCCGGGGAGCAGCGGCCATGA

Upstream 100 bases:

>100_bases
CGACTCCAACGCTTGAGCCGGCGCCATGATCACCACCCTGGATGCCCTCCCCCCAGACGATGTCCATGACCTCGTCGTCG
TCGGCTCCGGAGCCGGCGGA

Downstream 100 bases:

>100_bases
ACCCGCATCCGGGCCCCATCGCGCCCTCCGGCGCCGCACGCAATCACCCGACGAGAACCTACGACCTCGTGGTGATTGGC
TCGGGAGCGGGTGGCCTCGC

Product: putative FAD-binding dehydrogenase

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MATALFAAIAGLKVLLVERTEYVGGTTALSAATTWIPMNRHSSEVAVDDSLDKAEHFLDGVVGNRSPAVLRRAFLESGPQ
AIATLEDQSQVRFRPYATHPDYEQDHEGATLRGRALEPVPFDGRSLGADLARIRPPIPEFTIFGGMMIDRTDIGHLLGLT
KSWRSFRHAASILAWYGRDRLAQRRGSRLVMGNALIGRMLASLKARNVPILLEATVRAVDAGRGGVEGVTVAQNGAERQI
RVRRGLVIAAGGFNRHPTRRAELLPKPTPAYSPAAPGHTGAMQDLALKLGARFGTGGDQPAFWAPVSVRRRADGSTAVFP
HFVMDRSKPGTVCVDQTGRRFVNESTSYHQFGKAMFAANRDRPTIPCFIITDAVGLKRFGLGMVRMGTRNLKPFLDDGYL
VEAPTIAALAAKLNMDAAVLEETVGAMNTFAKTGVDTQFGRGTTAYHRINGDAAHGPNPTLGPIDTAPFYAVRLYPGDIG
AATGLVIDEWARVLKEDGSPVPGLYACGNEAQSIMGGTYPGPGITIGPAITFAYRAVRHACQAGEQRP

Sequences:

>Translated_548_residues
MATALFAAIAGLKVLLVERTEYVGGTTALSAATTWIPMNRHSSEVAVDDSLDKAEHFLDGVVGNRSPAVLRRAFLESGPQ
AIATLEDQSQVRFRPYATHPDYEQDHEGATLRGRALEPVPFDGRSLGADLARIRPPIPEFTIFGGMMIDRTDIGHLLGLT
KSWRSFRHAASILAWYGRDRLAQRRGSRLVMGNALIGRMLASLKARNVPILLEATVRAVDAGRGGVEGVTVAQNGAERQI
RVRRGLVIAAGGFNRHPTRRAELLPKPTPAYSPAAPGHTGAMQDLALKLGARFGTGGDQPAFWAPVSVRRRADGSTAVFP
HFVMDRSKPGTVCVDQTGRRFVNESTSYHQFGKAMFAANRDRPTIPCFIITDAVGLKRFGLGMVRMGTRNLKPFLDDGYL
VEAPTIAALAAKLNMDAAVLEETVGAMNTFAKTGVDTQFGRGTTAYHRINGDAAHGPNPTLGPIDTAPFYAVRLYPGDIG
AATGLVIDEWARVLKEDGSPVPGLYACGNEAQSIMGGTYPGPGITIGPAITFAYRAVRHACQAGEQRP
>Mature_547_residues
ATALFAAIAGLKVLLVERTEYVGGTTALSAATTWIPMNRHSSEVAVDDSLDKAEHFLDGVVGNRSPAVLRRAFLESGPQA
IATLEDQSQVRFRPYATHPDYEQDHEGATLRGRALEPVPFDGRSLGADLARIRPPIPEFTIFGGMMIDRTDIGHLLGLTK
SWRSFRHAASILAWYGRDRLAQRRGSRLVMGNALIGRMLASLKARNVPILLEATVRAVDAGRGGVEGVTVAQNGAERQIR
VRRGLVIAAGGFNRHPTRRAELLPKPTPAYSPAAPGHTGAMQDLALKLGARFGTGGDQPAFWAPVSVRRRADGSTAVFPH
FVMDRSKPGTVCVDQTGRRFVNESTSYHQFGKAMFAANRDRPTIPCFIITDAVGLKRFGLGMVRMGTRNLKPFLDDGYLV
EAPTIAALAAKLNMDAAVLEETVGAMNTFAKTGVDTQFGRGTTAYHRINGDAAHGPNPTLGPIDTAPFYAVRLYPGDIGA
ATGLVIDEWARVLKEDGSPVPGLYACGNEAQSIMGGTYPGPGITIGPAITFAYRAVRHACQAGEQRP

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 58845; Mature: 58714

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATALFAAIAGLKVLLVERTEYVGGTTALSAATTWIPMNRHSSEVAVDDSLDKAEHFLDG
CHHHHHHHHHHHEEEEEEHHHHCCCCHHHHHHEEEEECCCCCCCEEECCCHHHHHHHHHH
VVGNRSPAVLRRAFLESGPQAIATLEDQSQVRFRPYATHPDYEQDHEGATLRGRALEPVP
HCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCEEECCCCCCCC
FDGRSLGADLARIRPPIPEFTIFGGMMIDRTDIGHLLGLTKSWRSFRHAASILAWYGRDR
CCCHHHCCHHHHHCCCCCCEEEECCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAQRRGSRLVMGNALIGRMLASLKARNVPILLEATVRAVDAGRGGVEGVTVAQNGAERQI
HHHHCCCEEEECHHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCCCEEEEECCCCHHHE
RVRRGLVIAAGGFNRHPTRRAELLPKPTPAYSPAAPGHTGAMQDLALKLGARFGTGGDQP
EEECCEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
AFWAPVSVRRRADGSTAVFPHFVMDRSKPGTVCVDQTGRRFVNESTSYHQFGKAMFAANR
CEECCHHHHHCCCCCCEECHHHHHCCCCCCCEEECCCCHHHHHCCCCHHHHHHHHHHCCC
DRPTIPCFIITDAVGLKRFGLGMVRMGTRNLKPFLDDGYLVEAPTIAALAAKLNMDAAVL
CCCCCCEEEEECCCCHHHHCCCCEEECCCCCCCCCCCCEEEECCHHHHHHHHCCCHHHHH
EETVGAMNTFAKTGVDTQFGRGTTAYHRINGDAAHGPNPTLGPIDTAPFYAVRLYPGDIG
HHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
AATGLVIDEWARVLKEDGSPVPGLYACGNEAQSIMGGTYPGPGITIGPAITFAYRAVRHA
CHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHCCCCCCCCEEECHHHHHHHHHHHHH
CQAGEQRP
HHCCCCCC
>Mature Secondary Structure 
ATALFAAIAGLKVLLVERTEYVGGTTALSAATTWIPMNRHSSEVAVDDSLDKAEHFLDG
HHHHHHHHHHHEEEEEEHHHHCCCCHHHHHHEEEEECCCCCCCEEECCCHHHHHHHHHH
VVGNRSPAVLRRAFLESGPQAIATLEDQSQVRFRPYATHPDYEQDHEGATLRGRALEPVP
HCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCEEECCCCCCCC
FDGRSLGADLARIRPPIPEFTIFGGMMIDRTDIGHLLGLTKSWRSFRHAASILAWYGRDR
CCCHHHCCHHHHHCCCCCCEEEECCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAQRRGSRLVMGNALIGRMLASLKARNVPILLEATVRAVDAGRGGVEGVTVAQNGAERQI
HHHHCCCEEEECHHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCCCEEEEECCCCHHHE
RVRRGLVIAAGGFNRHPTRRAELLPKPTPAYSPAAPGHTGAMQDLALKLGARFGTGGDQP
EEECCEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
AFWAPVSVRRRADGSTAVFPHFVMDRSKPGTVCVDQTGRRFVNESTSYHQFGKAMFAANR
CEECCHHHHHCCCCCCEECHHHHHCCCCCCCEEECCCCHHHHHCCCCHHHHHHHHHHCCC
DRPTIPCFIITDAVGLKRFGLGMVRMGTRNLKPFLDDGYLVEAPTIAALAAKLNMDAAVL
CCCCCCEEEEECCCCHHHHCCCCEEECCCCCCCCCCCCEEEECCHHHHHHHHCCCHHHHH
EETVGAMNTFAKTGVDTQFGRGTTAYHRINGDAAHGPNPTLGPIDTAPFYAVRLYPGDIG
HHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
AATGLVIDEWARVLKEDGSPVPGLYACGNEAQSIMGGTYPGPGITIGPAITFAYRAVRHA
CHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHCCCCCCCCEEECHHHHHHHHHHHHH
CQAGEQRP
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]