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The map label for this gene is ptsP [H]

Identifier: 154246565

GI number: 154246565

Start: 2910157

End: 2912424

Strand: Reverse

Name: ptsP [H]

Synonym: Xaut_2624

Alternate gene names: 154246565

Gene position: 2912424-2910157 (Counterclockwise)

Preceding gene: 154246566

Following gene: 154246564

Centisome position: 54.86

GC content: 70.19

Gene sequence:

>2268_bases
ATGCGTGGCGCGCTCGGCGGTCCGCGCGTATTGCTCCGCCGCCTCCGCGAGGTGATGGCGGAACAGGTCAGCGCGCAGGA
CCGCCTCGACAAGATCGTGGTGCTGATCGCCGCCAACATGGTGGCCGAAGTCTGCTCGGTCTATGTGCTGCGCGTCGACC
AGACGCTCGAACTCTATGCCACGGAAGGCCTGAACCGGGACGCGGTTCATCTCACCGTCATGCGCGTGGATGAGGGCCTC
GTCGGCCATGTGGCGCGCGAGGCGGAATCCCTCGCTCTTTCGGACGCCCACGCCCATCCGGAATTCTCCTACCGCCCGGA
AACCGGCGAGGAGGTCTACAACTCCTTCCTCGGCGTGCCGATCCTGCGGGCCGGCAACACGCTGGGCGTGCTGGTGGTGC
AGAACCGCGCCCGCCGCACCTATACGGAAGAGGAGATCGAGGCGCTCCAGACCACCGCCATGGTGCTGGCGGAGATGATC
GCCTCCGGGGAACTCACCGCGCTGGCCAAGCCCGGCGCCGAGCCGGCGGTGCGCCGGCCCCTGCACCTGAAGGGCGTGGC
GCTGGCGGATGGCCTCGGGCTCGGCCACGTGGTGCTGCACGAGCCGCGCATCAAGGTCACCAACGTCATCGCCGACGACG
TTGTGAAGGAGACCGCCCGCCTCGATGCCGCCATCGGCAAGCTTCGGCACTCCATCGACCTGATGCTGGAGGACGACGGT
CTCGCCAAGGCCGGCGAGCATCGCGACATCCTGGAAGCCTACCGCATGTTCGCCCACGACCGGGGCTGGATGCACAAGAT
GCGTGAGGCGGTGCTCTCCGGCCTCACCGCCGAGGGCGCGGTGGAGCGGGTGCAGTCGGATACCCGCGCCCGCATGATGC
GCGCCTCCGACCCCTATCTGCGCGAGCGCCTGCACGACCTCGACGACCTCGCCAACCGCCTGCTGCGCGAGCTGACCGGC
CGCAATCGCGGCTCGGAGCGCGAGGCGCTGCCGGAAAACGCCATCCTCGTCGCCCGCAACATGAGCCCGGCGGCGCTGCT
CGACTATGACCGGACGCGCATCCGCGGCCTCGTGCTGGAGGAAGGTGGCACCACCAGCCACGTCACCATCGTCGCCCGCG
CGCTCGGCATCGCCGCCGTGGGCCAGGTGGAGAATGCGGCCGGCCTCGCCGATCCCGGCGACCCGGTCATCGTGGACGGG
CAGGCCGGCGAGGTGCATCTGCGCCCGCCCGGCGACGTTGAGGAAGCCTATGCGGAGAAGGTGCGCTTCCGCGCCCGCCG
CCAGGCCCAGTATGCGGCGCTGCGCACCCGCCCATCGGTCACCAAGGACGGCGTGCACATCGAGCTGCACCTGAATGCCG
GCCTGCTGGTGGACCTGCCCCATGTGTCCGAGACCGGCGCGGCGGGCATCGGCCTGTTCCGCACCGAGCTGCAATTCATG
ATCGCCTCGGCCTTCCCGCGCATCTCGGAACAGCTGAAGCTCTATCGCGCGGTGCTGGATGCCGCCGGCGAAAAGCCCGT
CACCTTCCGCACCCTCGACATCGGCGGCGACAAGGTGCTGCCCTATATGCGCACCGTCGAGGAGGAGAACCCGGCGCTGG
GCTGGCGCGCCATCCGCCTCGGGCTGGACCGGCCGGGCCTGCTGCGCAGCCAGCTGCGGGCGCTGCTGCGGGCCGGCGCG
GGGCGCGATCTGCGCATCATGTTCCCCATGGTGGCGGCGGTGGAGGAATTCGACCGCGCCCGCGCCATCGTCGAGCGCGA
GCTGACCCATCTGCGCAAGCACGGCCACGGCCTGCCCGAGAAGGTCTATGTGGGCGCCATGGTGGAGGTGCCCTCCCTGC
TGTTCCAGCTGGACGAGATCCTCTCGCGCGCCGACTTCCTCTCGGTGGGCTCCAATGATCTGGTCCAGTTCCTCTATGCG
GCGGACCGCTCCAACGTGCGCGTGGCGGACCGCTTCGACCCGCTGTCCCCGCCGGCCCTTCGGGCGTTCCGCATGGTGGC
GGAGGCGGGCCAGCGCCATGGCAAGCCGGTGACCCTGTGCGGCGAACTGGCATCGCGGCCGCTTGAGGCGGTGGCCCTCG
CTGCGCTGGGATACCGGGCACTGTCGGTGTCGCCGGCCTCCATTGGCCCGGTGAAGGCCATGATGCTGGAGCTGGATGTC
TCCGCCGCCCGCAAGGTGCTGCTGCCCCTGCTGGAGGACAAGACAGGCACGGCCGACCCGCGCGCCGCGCTCGCAGCTTT
CGCCAAGGCGAGCGGGCTGCCCATTTAG

Upstream 100 bases:

>100_bases
ATACGGGCTGGACAGCTGATGGGCGAACCTGCGGGCGCGCGCCGTTCGACGAAGAGCCCCGCGGGCACCTGAAACCCGCC
CACATTTAAGGATGCGGCCG

Downstream 100 bases:

>100_bases
GGCGCGCCCAAACGGCCGCCCCCACCCGCGTTTTCTGGACGCAAACCGGCTTCACCCTTGCGGAAAACGCCCGAGACCCA
TGACCGCCCTGCCCACCGCC

Product: phosphoenolpyruvate-protein phosphotransferase PtsP

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 755; Mature: 755

Protein sequence:

>755_residues
MRGALGGPRVLLRRLREVMAEQVSAQDRLDKIVVLIAANMVAEVCSVYVLRVDQTLELYATEGLNRDAVHLTVMRVDEGL
VGHVAREAESLALSDAHAHPEFSYRPETGEEVYNSFLGVPILRAGNTLGVLVVQNRARRTYTEEEIEALQTTAMVLAEMI
ASGELTALAKPGAEPAVRRPLHLKGVALADGLGLGHVVLHEPRIKVTNVIADDVVKETARLDAAIGKLRHSIDLMLEDDG
LAKAGEHRDILEAYRMFAHDRGWMHKMREAVLSGLTAEGAVERVQSDTRARMMRASDPYLRERLHDLDDLANRLLRELTG
RNRGSEREALPENAILVARNMSPAALLDYDRTRIRGLVLEEGGTTSHVTIVARALGIAAVGQVENAAGLADPGDPVIVDG
QAGEVHLRPPGDVEEAYAEKVRFRARRQAQYAALRTRPSVTKDGVHIELHLNAGLLVDLPHVSETGAAGIGLFRTELQFM
IASAFPRISEQLKLYRAVLDAAGEKPVTFRTLDIGGDKVLPYMRTVEEENPALGWRAIRLGLDRPGLLRSQLRALLRAGA
GRDLRIMFPMVAAVEEFDRARAIVERELTHLRKHGHGLPEKVYVGAMVEVPSLLFQLDEILSRADFLSVGSNDLVQFLYA
ADRSNVRVADRFDPLSPPALRAFRMVAEAGQRHGKPVTLCGELASRPLEAVALAALGYRALSVSPASIGPVKAMMLELDV
SAARKVLLPLLEDKTGTADPRAALAAFAKASGLPI

Sequences:

>Translated_755_residues
MRGALGGPRVLLRRLREVMAEQVSAQDRLDKIVVLIAANMVAEVCSVYVLRVDQTLELYATEGLNRDAVHLTVMRVDEGL
VGHVAREAESLALSDAHAHPEFSYRPETGEEVYNSFLGVPILRAGNTLGVLVVQNRARRTYTEEEIEALQTTAMVLAEMI
ASGELTALAKPGAEPAVRRPLHLKGVALADGLGLGHVVLHEPRIKVTNVIADDVVKETARLDAAIGKLRHSIDLMLEDDG
LAKAGEHRDILEAYRMFAHDRGWMHKMREAVLSGLTAEGAVERVQSDTRARMMRASDPYLRERLHDLDDLANRLLRELTG
RNRGSEREALPENAILVARNMSPAALLDYDRTRIRGLVLEEGGTTSHVTIVARALGIAAVGQVENAAGLADPGDPVIVDG
QAGEVHLRPPGDVEEAYAEKVRFRARRQAQYAALRTRPSVTKDGVHIELHLNAGLLVDLPHVSETGAAGIGLFRTELQFM
IASAFPRISEQLKLYRAVLDAAGEKPVTFRTLDIGGDKVLPYMRTVEEENPALGWRAIRLGLDRPGLLRSQLRALLRAGA
GRDLRIMFPMVAAVEEFDRARAIVERELTHLRKHGHGLPEKVYVGAMVEVPSLLFQLDEILSRADFLSVGSNDLVQFLYA
ADRSNVRVADRFDPLSPPALRAFRMVAEAGQRHGKPVTLCGELASRPLEAVALAALGYRALSVSPASIGPVKAMMLELDV
SAARKVLLPLLEDKTGTADPRAALAAFAKASGLPI
>Mature_755_residues
MRGALGGPRVLLRRLREVMAEQVSAQDRLDKIVVLIAANMVAEVCSVYVLRVDQTLELYATEGLNRDAVHLTVMRVDEGL
VGHVAREAESLALSDAHAHPEFSYRPETGEEVYNSFLGVPILRAGNTLGVLVVQNRARRTYTEEEIEALQTTAMVLAEMI
ASGELTALAKPGAEPAVRRPLHLKGVALADGLGLGHVVLHEPRIKVTNVIADDVVKETARLDAAIGKLRHSIDLMLEDDG
LAKAGEHRDILEAYRMFAHDRGWMHKMREAVLSGLTAEGAVERVQSDTRARMMRASDPYLRERLHDLDDLANRLLRELTG
RNRGSEREALPENAILVARNMSPAALLDYDRTRIRGLVLEEGGTTSHVTIVARALGIAAVGQVENAAGLADPGDPVIVDG
QAGEVHLRPPGDVEEAYAEKVRFRARRQAQYAALRTRPSVTKDGVHIELHLNAGLLVDLPHVSETGAAGIGLFRTELQFM
IASAFPRISEQLKLYRAVLDAAGEKPVTFRTLDIGGDKVLPYMRTVEEENPALGWRAIRLGLDRPGLLRSQLRALLRAGA
GRDLRIMFPMVAAVEEFDRARAIVERELTHLRKHGHGLPEKVYVGAMVEVPSLLFQLDEILSRADFLSVGSNDLVQFLYA
ADRSNVRVADRFDPLSPPALRAFRMVAEAGQRHGKPVTLCGELASRPLEAVALAALGYRALSVSPASIGPVKAMMLELDV
SAARKVLLPLLEDKTGTADPRAALAAFAKASGLPI

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=752, Percent_Identity=36.436170212766, Blast_Score=390, Evalue=1e-109,
Organism=Escherichia coli, GI1788726, Length=555, Percent_Identity=32.7927927927928, Blast_Score=287, Evalue=2e-78,
Organism=Escherichia coli, GI1788756, Length=549, Percent_Identity=32.9690346083789, Blast_Score=286, Evalue=3e-78,
Organism=Escherichia coli, GI48994992, Length=493, Percent_Identity=34.4827586206897, Blast_Score=283, Evalue=4e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 82359; Mature: 82359

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGALGGPRVLLRRLREVMAEQVSAQDRLDKIVVLIAANMVAEVCSVYVLRVDQTLELYA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEHH
TEGLNRDAVHLTVMRVDEGLVGHVAREAESLALSDAHAHPEFSYRPETGEEVYNSFLGVP
HCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCE
ILRAGNTLGVLVVQNRARRTYTEEEIEALQTTAMVLAEMIASGELTALAKPGAEPAVRRP
EEECCCEEEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCC
LHLKGVALADGLGLGHVVLHEPRIKVTNVIADDVVKETARLDAAIGKLRHSIDLMLEDDG
CEECCEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCC
LAKAGEHRDILEAYRMFAHDRGWMHKMREAVLSGLTAEGAVERVQSDTRARMMRASDPYL
CCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHH
RERLHDLDDLANRLLRELTGRNRGSEREALPENAILVARNMSPAALLDYDRTRIRGLVLE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCHHHHEEEEEE
EGGTTSHVTIVARALGIAAVGQVENAAGLADPGDPVIVDGQAGEVHLRPPGDVEEAYAEK
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCHHHHHHHH
VRFRARRQAQYAALRTRPSVTKDGVHIELHLNAGLLVDLPHVSETGAAGIGLFRTELQFM
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHH
IASAFPRISEQLKLYRAVLDAAGEKPVTFRTLDIGGDKVLPYMRTVEEENPALGWRAIRL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCEEEEE
GLDRPGLLRSQLRALLRAGAGRDLRIMFPMVAAVEEFDRARAIVERELTHLRKHGHGLPE
CCCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KVYVGAMVEVPSLLFQLDEILSRADFLSVGSNDLVQFLYAADRSNVRVADRFDPLSPPAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEECCCCCCCCHHH
RAFRMVAEAGQRHGKPVTLCGELASRPLEAVALAALGYRALSVSPASIGPVKAMMLELDV
HHHHHHHHHHHHCCCCEEEHHHHHCCHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHH
SAARKVLLPLLEDKTGTADPRAALAAFAKASGLPI
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRGALGGPRVLLRRLREVMAEQVSAQDRLDKIVVLIAANMVAEVCSVYVLRVDQTLELYA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEHH
TEGLNRDAVHLTVMRVDEGLVGHVAREAESLALSDAHAHPEFSYRPETGEEVYNSFLGVP
HCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCE
ILRAGNTLGVLVVQNRARRTYTEEEIEALQTTAMVLAEMIASGELTALAKPGAEPAVRRP
EEECCCEEEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCC
LHLKGVALADGLGLGHVVLHEPRIKVTNVIADDVVKETARLDAAIGKLRHSIDLMLEDDG
CEECCEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCC
LAKAGEHRDILEAYRMFAHDRGWMHKMREAVLSGLTAEGAVERVQSDTRARMMRASDPYL
CCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHH
RERLHDLDDLANRLLRELTGRNRGSEREALPENAILVARNMSPAALLDYDRTRIRGLVLE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCHHHHEEEEEE
EGGTTSHVTIVARALGIAAVGQVENAAGLADPGDPVIVDGQAGEVHLRPPGDVEEAYAEK
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCHHHHHHHH
VRFRARRQAQYAALRTRPSVTKDGVHIELHLNAGLLVDLPHVSETGAAGIGLFRTELQFM
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHH
IASAFPRISEQLKLYRAVLDAAGEKPVTFRTLDIGGDKVLPYMRTVEEENPALGWRAIRL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCEEEEE
GLDRPGLLRSQLRALLRAGAGRDLRIMFPMVAAVEEFDRARAIVERELTHLRKHGHGLPE
CCCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KVYVGAMVEVPSLLFQLDEILSRADFLSVGSNDLVQFLYAADRSNVRVADRFDPLSPPAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEECCCCCCCCHHH
RAFRMVAEAGQRHGKPVTLCGELASRPLEAVALAALGYRALSVSPASIGPVKAMMLELDV
HHHHHHHHHHHHCCCCEEEHHHHHCCHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHH
SAARKVLLPLLEDKTGTADPRAALAAFAKASGLPI
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]