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The map label for this gene is prfA

Identifier: 154246564

GI number: 154246564

Start: 2908995

End: 2910077

Strand: Reverse

Name: prfA

Synonym: Xaut_2623

Alternate gene names: 154246564

Gene position: 2910077-2908995 (Counterclockwise)

Preceding gene: 154246565

Following gene: 154246563

Centisome position: 54.81

GC content: 70.27

Gene sequence:

>1083_bases
ATGACCGCCCTGCCCACCGCCAAGCTCGACCAGTTGGTCGCGCGCCACGCCGAGATCGAGGCCGCCCTGTCCCGCGGCGC
GGAGGGCGAGGAATATGTGCGCCTCGGCCGCGAGTTTTCCGACCTGTCGCCGGTGGTGGAGCAGGTGCGCGCCCTGAAGG
ATGCCGAGCGCGACCTGGAAGGCGCCAATGAGCTTCTGGCCGAATCCGCCGCCGACCCCGAGATGCGGGAAATGGCCGAG
GCGGAGGTGGAACGCCTTCAAGACCGGATTGAGGCCCTTGCCACCGCCGTGCGCCTCGCGCTGCTGCCCAAGGACGCCAT
GGACGAGCGCGGCATCATCCTGGAGGTGCGCGCCGGCACCGGCGGCGACGAGGCCGCCCTGTTCGCGGGCGATCTCTACC
GGATGTACACGCGCTACGCCGCCGAGAAGGGCTGGAGCGTGGAAATCCTCTCCGAGAGCGAGGGGACCATGGGCGGCTAC
AAGGAAATCGTCGCCGCCCTTCACGGCCGCGGCGCCTATGCGCGCCTGAAGTTCGAGAGCGGCGTCCACCGGGTCCAGCG
CGTGCCGGCCACCGAGACCAGCGGGCGCATCCACACCTCCGCCGCCACCGTCGCCGTGCTGCCCGAGGCGGAGGACGTGG
ACGTGGACATCAACGAGGCGGACCTGCGCATCGACGTGTATCGCGCCCAGGGCGCCGGCGGCCAGCATGTGAACAAGACC
GAGAGCGCGGTGCGCATCACCCACCTTCCCACCAACACGGTGGTGGCGGTGCAGGACGAGCGCTCCCAGCACCGCAACAA
GGCCCGCGCCATGGCGCTGCTGCGTGCGCGCCTGCTCGACGACGAGCGCCAGAAGAAGGAGGCCGGCCGCGCCGCCGAGC
GCAAGGGCCAGGTGGGCTCCGGCGACCGCTCCGAGCGCATCCGCACCTATAATTTCCCCCAAGGCCGCGTCACCGACCAC
CGCATCAACCTCACCCTCTACAAGCTGGACGAGGTGATCGCGGGCACGGCGCTGGACGAGATCATCGATCCGCTGCTCAC
CGAACACCAGGCCGCTTTGCTCGCCGCGGCCGAGGACGCCTGA

Upstream 100 bases:

>100_bases
GCGAGCGGGCTGCCCATTTAGGGCGCGCCCAAACGGCCGCCCCCACCCGCGTTTTCTGGACGCAAACCGGCTTCACCCTT
GCGGAAAACGCCCGAGACCC

Downstream 100 bases:

>100_bases
GCCGTGGGCAAGGGCCCTGCTTGTGGCTGAAGCCCTGACCATCGGCACTCTGCGCCGGCAGATGGCGGCATCCCTCGCTG
CGGCGGGCGTGGAGACGCCG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MTALPTAKLDQLVARHAEIEAALSRGAEGEEYVRLGREFSDLSPVVEQVRALKDAERDLEGANELLAESAADPEMREMAE
AEVERLQDRIEALATAVRLALLPKDAMDERGIILEVRAGTGGDEAALFAGDLYRMYTRYAAEKGWSVEILSESEGTMGGY
KEIVAALHGRGAYARLKFESGVHRVQRVPATETSGRIHTSAATVAVLPEAEDVDVDINEADLRIDVYRAQGAGGQHVNKT
ESAVRITHLPTNTVVAVQDERSQHRNKARAMALLRARLLDDERQKKEAGRAAERKGQVGSGDRSERIRTYNFPQGRVTDH
RINLTLYKLDEVIAGTALDEIIDPLLTEHQAALLAAAEDA

Sequences:

>Translated_360_residues
MTALPTAKLDQLVARHAEIEAALSRGAEGEEYVRLGREFSDLSPVVEQVRALKDAERDLEGANELLAESAADPEMREMAE
AEVERLQDRIEALATAVRLALLPKDAMDERGIILEVRAGTGGDEAALFAGDLYRMYTRYAAEKGWSVEILSESEGTMGGY
KEIVAALHGRGAYARLKFESGVHRVQRVPATETSGRIHTSAATVAVLPEAEDVDVDINEADLRIDVYRAQGAGGQHVNKT
ESAVRITHLPTNTVVAVQDERSQHRNKARAMALLRARLLDDERQKKEAGRAAERKGQVGSGDRSERIRTYNFPQGRVTDH
RINLTLYKLDEVIAGTALDEIIDPLLTEHQAALLAAAEDA
>Mature_359_residues
TALPTAKLDQLVARHAEIEAALSRGAEGEEYVRLGREFSDLSPVVEQVRALKDAERDLEGANELLAESAADPEMREMAEA
EVERLQDRIEALATAVRLALLPKDAMDERGIILEVRAGTGGDEAALFAGDLYRMYTRYAAEKGWSVEILSESEGTMGGYK
EIVAALHGRGAYARLKFESGVHRVQRVPATETSGRIHTSAATVAVLPEAEDVDVDINEADLRIDVYRAQGAGGQHVNKTE
SAVRITHLPTNTVVAVQDERSQHRNKARAMALLRARLLDDERQKKEAGRAAERKGQVGSGDRSERIRTYNFPQGRVTDHR
INLTLYKLDEVIAGTALDEIIDPLLTEHQAALLAAAEDA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=341, Percent_Identity=44.574780058651, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI34577120, Length=347, Percent_Identity=38.9048991354467, Blast_Score=241, Evalue=5e-64,
Organism=Homo sapiens, GI166795305, Length=247, Percent_Identity=42.5101214574899, Blast_Score=197, Evalue=1e-50,
Organism=Escherichia coli, GI1787462, Length=354, Percent_Identity=57.909604519774, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI2367172, Length=315, Percent_Identity=41.2698412698413, Blast_Score=212, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI17542784, Length=314, Percent_Identity=36.3057324840764, Blast_Score=195, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6321295, Length=362, Percent_Identity=40.0552486187845, Blast_Score=249, Evalue=4e-67,
Organism=Drosophila melanogaster, GI19921226, Length=322, Percent_Identity=44.7204968944099, Blast_Score=233, Evalue=1e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_XANP2 (A7IIM2)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001417522.1
- ProteinModelPortal:   A7IIM2
- SMR:   A7IIM2
- STRING:   A7IIM2
- GeneID:   5422475
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_2623
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   XAUT78245:XAUT_2623-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 39438; Mature: 39307

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALPTAKLDQLVARHAEIEAALSRGAEGEEYVRLGREFSDLSPVVEQVRALKDAERDLE
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
GANELLAESAADPEMREMAEAEVERLQDRIEALATAVRLALLPKDAMDERGIILEVRAGT
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCC
GGDEAALFAGDLYRMYTRYAAEKGWSVEILSESEGTMGGYKEIVAALHGRGAYARLKFES
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEHHH
GVHRVQRVPATETSGRIHTSAATVAVLPEAEDVDVDINEADLRIDVYRAQGAGGQHVNKT
HHHHHHHCCCCCCCCEEEECCEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCC
ESAVRITHLPTNTVVAVQDERSQHRNKARAMALLRARLLDDERQKKEAGRAAERKGQVGS
CCEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCC
GDRSERIRTYNFPQGRVTDHRINLTLYKLDEVIAGTALDEIIDPLLTEHQAALLAAAEDA
CCCCCCCEEECCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TALPTAKLDQLVARHAEIEAALSRGAEGEEYVRLGREFSDLSPVVEQVRALKDAERDLE
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
GANELLAESAADPEMREMAEAEVERLQDRIEALATAVRLALLPKDAMDERGIILEVRAGT
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCC
GGDEAALFAGDLYRMYTRYAAEKGWSVEILSESEGTMGGYKEIVAALHGRGAYARLKFES
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEHHH
GVHRVQRVPATETSGRIHTSAATVAVLPEAEDVDVDINEADLRIDVYRAQGAGGQHVNKT
HHHHHHHCCCCCCCCEEEECCEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCC
ESAVRITHLPTNTVVAVQDERSQHRNKARAMALLRARLLDDERQKKEAGRAAERKGQVGS
CCEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCC
GDRSERIRTYNFPQGRVTDHRINLTLYKLDEVIAGTALDEIIDPLLTEHQAALLAAAEDA
CCCCCCCEEECCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA