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The map label for this gene is puuA [H]

Identifier: 154246278

GI number: 154246278

Start: 2609474

End: 2610874

Strand: Direct

Name: puuA [H]

Synonym: Xaut_2336

Alternate gene names: 154246278

Gene position: 2609474-2610874 (Clockwise)

Preceding gene: 154246274

Following gene: 154246279

Centisome position: 49.15

GC content: 70.31

Gene sequence:

>1401_bases
ATGCAGGCCCAGGTTCCAGGCCGCCGACCCGACCCTGCCGCGGCCCTCGGGGTCACGGACGAGGCGGCGGATTATGTGAC
AGCCTTCGGCGTGCCGGAGGTGGTGGAGGTTCTTCTGCCCGACACCTCCGCGGTGCTGCGGGGCAAGTGGATTCCCGGCC
ATTCCCTGGGCAAGATCTGGAAGGACGGCGTGGCCATCCCGCTCTCCATCTTCGGCCTCGATGTCTGGGGCTGCGAGGTG
GACGCCACCGAGATCCACATGGAGACCGGCGACAAGGACGGCCTGTGCTGGCCGGTGCCGGGGACGCTGAAGCCGGTGCC
CTGGGCGTCGCGCCACACCGCGCAGGTGCTCATCACCATGCACGAGCCCGACGGCTCGGCCCGCGGCAGGCCGTGGAAGC
TCGATCCGCGCCAGCAACTCGCCGCCGTGGTGGAGCGCTTCGCCGCGCGCGGGCTCACCCCCTGCGTCGCCTTCGAACTG
GAATTCTACCTGCTGAAGCCGTCGGACGTGCCGGGCGCGCCGCCCGAGCCGGTGTTCCAGGCCACCGGGCAGGCGCGGCA
GAACATGTACGCCATGTCCGACCTCGACGCCTACGCCGAGGTCCTGCACGACATGCGCGCCGCGTGCGTCACGCAGGGCC
TGCCCGCCGATACCGTGATCGCGGAGGCGGCGCCCGGCCAGTTCGAGGTCAATCTCTACCACAAGGTGGATGCGCTCGGC
GCCGCCGACGACGCGGTGCTGCTGCGCCGGCTGATCGACGGCGTGGCACGCCGCCATGGGCTGAAGGCCACCTTCATGGC
CAAGCCGCTGGCGGATTTCGCCGGCAACGGGATGCATGTCCATGCCAGCCTGATGGAGCATGGCGGCGGCAACCTGTTCG
CCCGCGAGGACGGGGTGGGCGACACCGCCCTGCGCCATGCCGCGGGCGGCATGCTGGCCAGCATGGCCGACGCCACGCTC
ATTTTCGTGCCGAGCTTCAACGGCTATCGCCGGCTAGTGCCGGGCAGCTACGCGCCGACCTCGGCGAGCTGGGGCTTCGA
CAACCGGCTGGTGGCGGTGCGCGTGCCCAACGGCCCGCGCCACGCCCGCCGGCTGGAGCACCGCATCGCCGGGGCGGAGG
CTCACCCCCACCTCGTGCTCGCGGCCATCCTCGCCGGCATGCTGGACGGGCTGGAGCGGGGCATCGAGCCGCCGCCGCCG
CTGGAAGGCAACGCCTACGAGCAGATCGGCGAGCCCCTGTCGCCAGATATGGAGGTGGCGGTGGACCGCTTCGCCGCCTC
CGACTTCATCGCCCGCACCTTCGGGGAGGACTACCGGCGCATCTATGAGGCCATGAAGCGGGAGGAATTGGCTGCATTCC
GCAGGGTCATCCTTCCATTGGAGTACGAAACGTATTTGTGA

Upstream 100 bases:

>100_bases
TGTCCGGTACCGCTCCAGTTGCATAATTATCGTCGCGCTTGAAGGTTCCCGAGCTTCGCGCTTGGATGAACAGGAAATCG
ACTTTTTTGAATGGAGTGTC

Downstream 100 bases:

>100_bases
AGCGTGGCGTTTCCTGTTGAGGGGGCTCGCGCGACAACATAGCCTTGGGTCCAGGGGGGCACCTTCAGGGCAACCTTGCA
AAACATGACCCCCTTTCCAG

Product: glutamine synthetase catalytic subunit

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MQAQVPGRRPDPAAALGVTDEAADYVTAFGVPEVVEVLLPDTSAVLRGKWIPGHSLGKIWKDGVAIPLSIFGLDVWGCEV
DATEIHMETGDKDGLCWPVPGTLKPVPWASRHTAQVLITMHEPDGSARGRPWKLDPRQQLAAVVERFAARGLTPCVAFEL
EFYLLKPSDVPGAPPEPVFQATGQARQNMYAMSDLDAYAEVLHDMRAACVTQGLPADTVIAEAAPGQFEVNLYHKVDALG
AADDAVLLRRLIDGVARRHGLKATFMAKPLADFAGNGMHVHASLMEHGGGNLFAREDGVGDTALRHAAGGMLASMADATL
IFVPSFNGYRRLVPGSYAPTSASWGFDNRLVAVRVPNGPRHARRLEHRIAGAEAHPHLVLAAILAGMLDGLERGIEPPPP
LEGNAYEQIGEPLSPDMEVAVDRFAASDFIARTFGEDYRRIYEAMKREELAAFRRVILPLEYETYL

Sequences:

>Translated_466_residues
MQAQVPGRRPDPAAALGVTDEAADYVTAFGVPEVVEVLLPDTSAVLRGKWIPGHSLGKIWKDGVAIPLSIFGLDVWGCEV
DATEIHMETGDKDGLCWPVPGTLKPVPWASRHTAQVLITMHEPDGSARGRPWKLDPRQQLAAVVERFAARGLTPCVAFEL
EFYLLKPSDVPGAPPEPVFQATGQARQNMYAMSDLDAYAEVLHDMRAACVTQGLPADTVIAEAAPGQFEVNLYHKVDALG
AADDAVLLRRLIDGVARRHGLKATFMAKPLADFAGNGMHVHASLMEHGGGNLFAREDGVGDTALRHAAGGMLASMADATL
IFVPSFNGYRRLVPGSYAPTSASWGFDNRLVAVRVPNGPRHARRLEHRIAGAEAHPHLVLAAILAGMLDGLERGIEPPPP
LEGNAYEQIGEPLSPDMEVAVDRFAASDFIARTFGEDYRRIYEAMKREELAAFRRVILPLEYETYL
>Mature_466_residues
MQAQVPGRRPDPAAALGVTDEAADYVTAFGVPEVVEVLLPDTSAVLRGKWIPGHSLGKIWKDGVAIPLSIFGLDVWGCEV
DATEIHMETGDKDGLCWPVPGTLKPVPWASRHTAQVLITMHEPDGSARGRPWKLDPRQQLAAVVERFAARGLTPCVAFEL
EFYLLKPSDVPGAPPEPVFQATGQARQNMYAMSDLDAYAEVLHDMRAACVTQGLPADTVIAEAAPGQFEVNLYHKVDALG
AADDAVLLRRLIDGVARRHGLKATFMAKPLADFAGNGMHVHASLMEHGGGNLFAREDGVGDTALRHAAGGMLASMADATL
IFVPSFNGYRRLVPGSYAPTSASWGFDNRLVAVRVPNGPRHARRLEHRIAGAEAHPHLVLAAILAGMLDGLERGIEPPPP
LEGNAYEQIGEPLSPDMEVAVDRFAASDFIARTFGEDYRRIYEAMKREELAAFRRVILPLEYETYL

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=433, Percent_Identity=22.8637413394919, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI87081870, Length=451, Percent_Identity=39.4678492239468, Blast_Score=324, Evalue=6e-90,
Organism=Escherichia coli, GI1790301, Length=241, Percent_Identity=32.7800829875519, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50510; Mature: 50510

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAQVPGRRPDPAAALGVTDEAADYVTAFGVPEVVEVLLPDTSAVLRGKWIPGHSLGKIW
CCCCCCCCCCCCCCEECCCHHHHHHHHHCCHHHHHHHHCCCCHHHHCCCCCCCCHHHHHH
KDGVAIPLSIFGLDVWGCEVDATEIHMETGDKDGLCWPVPGTLKPVPWASRHTAQVLITM
HCCCEEEEEEEEEEEECCCCCCEEEEEECCCCCCEEECCCCCCCCCCCCCCCCEEEEEEE
HEPDGSARGRPWKLDPRQQLAAVVERFAARGLTPCVAFELEFYLLKPSDVPGAPPEPVFQ
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCHHHH
ATGQARQNMYAMSDLDAYAEVLHDMRAACVTQGLPADTVIAEAAPGQFEVNLYHKVDALG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEECCCCCCEEEEEEEEHHHCC
AADDAVLLRRLIDGVARRHGLKATFMAKPLADFAGNGMHVHASLMEHGGGNLFAREDGVG
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEHHHCCCCCEEEECCCCC
DTALRHAAGGMLASMADATLIFVPSFNGYRRLVPGSYAPTSASWGFDNRLVAVRVPNGPR
HHHHHHHHCHHHHHHCCEEEEEEECCCCCEECCCCCCCCCCCCCCCCCCEEEEECCCCHH
HARRLEHRIAGAEAHPHLVLAAILAGMLDGLERGIEPPPPLEGNAYEQIGEPLSPDMEVA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHH
VDRFAASDFIARTFGEDYRRIYEAMKREELAAFRRVILPLEYETYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCC
>Mature Secondary Structure
MQAQVPGRRPDPAAALGVTDEAADYVTAFGVPEVVEVLLPDTSAVLRGKWIPGHSLGKIW
CCCCCCCCCCCCCCEECCCHHHHHHHHHCCHHHHHHHHCCCCHHHHCCCCCCCCHHHHHH
KDGVAIPLSIFGLDVWGCEVDATEIHMETGDKDGLCWPVPGTLKPVPWASRHTAQVLITM
HCCCEEEEEEEEEEEECCCCCCEEEEEECCCCCCEEECCCCCCCCCCCCCCCCEEEEEEE
HEPDGSARGRPWKLDPRQQLAAVVERFAARGLTPCVAFELEFYLLKPSDVPGAPPEPVFQ
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCHHHH
ATGQARQNMYAMSDLDAYAEVLHDMRAACVTQGLPADTVIAEAAPGQFEVNLYHKVDALG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEECCCCCCEEEEEEEEHHHCC
AADDAVLLRRLIDGVARRHGLKATFMAKPLADFAGNGMHVHASLMEHGGGNLFAREDGVG
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEHHHCCCCCEEEECCCCC
DTALRHAAGGMLASMADATLIFVPSFNGYRRLVPGSYAPTSASWGFDNRLVAVRVPNGPR
HHHHHHHHCHHHHHHCCEEEEEEECCCCCEECCCCCCCCCCCCCCCCCCEEEEECCCCHH
HARRLEHRIAGAEAHPHLVLAAILAGMLDGLERGIEPPPPLEGNAYEQIGEPLSPDMEVA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHH
VDRFAASDFIARTFGEDYRRIYEAMKREELAAFRRVILPLEYETYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]