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The map label for this gene is potF [H]

Identifier: 154246279

GI number: 154246279

Start: 2610988

End: 2612094

Strand: Direct

Name: potF [H]

Synonym: Xaut_2337

Alternate gene names: 154246279

Gene position: 2610988-2612094 (Clockwise)

Preceding gene: 154246278

Following gene: 154246282

Centisome position: 49.18

GC content: 59.53

Gene sequence:

>1107_bases
ATGCGTCGTCATTTCGTAGCGGCAATCATGTGTGCCACGCTGGGCTGGGCCGGCGCGGCTTCGGCCCAGGACAAGGTCCT
CAACGTCTTCAACTGGTCGGATTATATCGACCCGACGGTGCTGGAGGACTTCACCAAGGAAACCGGCATCAAAGTCCGCT
ACGACGTCTTCGACAGCAACGAGGTGCTGGAGACGAAGCTGCTCGCGGGCAAGACCGGCTACGACGTGGTGGTGCCGTCC
GGCTCCTTCCTGCAGCGCCAGATCAAGGCCGGCATCTTCCTGACCCTCGACAAGTCGAAGATCCCGAACCTGCAATACGC
CTGGCCCGAGGTGACCAAGCGCCTCGCCGTCTACGACCCGGACAATGCCCACGCCGTCAACTACATGTGGGGCACCACGG
GCATCGGCTACAATGTGGACAAGGTAAAGGAACGGCTCGGCGACATTCCCATGAATACGTGGGACATCGTGTTCAAGCCG
GAAATCCTCTCCAAGCTCAAGGATTGCGGCGTCTATTTCCTCGACGGCCCGGAGGAGGTGATCCCCTCCGCCATGAAGTA
TCTGGGGCTCGATCCCAATTCGAAGAACCCGGACGACATCGCCAAGGCCGGCGAACTGCTCATGTCGGTTCGCCCCTATA
TCAAGAAGTTCGATTCCTCGGGCTATATCAATGCCCTGGCGCGCGGCGACATCTGTCTGGCGGTGGGCTGGTCGGGTGAC
GTGTTCCAGGCCAAGACCCGCGCCGCCGAGGCCGCGAAGAAGACCAAGAAGCCGCCGGTGAACGTGGCCTACGTGATCCC
GAAGCAGGGCGCGCTCATGTGGTTCGACAATTTCGCCATCCCCAAGGATGCGAAGAATATCGACAATGCCCTCGTCTTCA
TCAACTACATGATGCGGCCGGAAGTGGCGGCGAAGAACTCGAACTTCATTTCCTATGCCAACGGCAACCTGGAATCGCAG
AAGTTCATCGACAAGGACATCCTGAACAACCCGTCCATCTACCCGGACAAGGAGACGTTCGAGCGGCTGTTCATCGTCAC
CACCAACGAGCCTCCGGTGCAGCGGGTCATCACCAAGACCTGGAACAGCTTCAAGCGCGGCAAGTGA

Upstream 100 bases:

>100_bases
CTGTTGAGGGGGCTCGCGCGACAACATAGCCTTGGGTCCAGGGGGGCACCTTCAGGGCAACCTTGCAAAACATGACCCCC
TTTCCAGAGGAGAACGGCGG

Downstream 100 bases:

>100_bases
GGGGCGGGGCTCGCTTGAACGGCTGAGCCGGACGGTCGGCCCAAACGAAAAGACCACCGGAAGCCCCCGCTTCCGGTGGT
CTTTGCTTGTGTGGCCGCAC

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MRRHFVAAIMCATLGWAGAASAQDKVLNVFNWSDYIDPTVLEDFTKETGIKVRYDVFDSNEVLETKLLAGKTGYDVVVPS
GSFLQRQIKAGIFLTLDKSKIPNLQYAWPEVTKRLAVYDPDNAHAVNYMWGTTGIGYNVDKVKERLGDIPMNTWDIVFKP
EILSKLKDCGVYFLDGPEEVIPSAMKYLGLDPNSKNPDDIAKAGELLMSVRPYIKKFDSSGYINALARGDICLAVGWSGD
VFQAKTRAAEAAKKTKKPPVNVAYVIPKQGALMWFDNFAIPKDAKNIDNALVFINYMMRPEVAAKNSNFISYANGNLESQ
KFIDKDILNNPSIYPDKETFERLFIVTTNEPPVQRVITKTWNSFKRGK

Sequences:

>Translated_368_residues
MRRHFVAAIMCATLGWAGAASAQDKVLNVFNWSDYIDPTVLEDFTKETGIKVRYDVFDSNEVLETKLLAGKTGYDVVVPS
GSFLQRQIKAGIFLTLDKSKIPNLQYAWPEVTKRLAVYDPDNAHAVNYMWGTTGIGYNVDKVKERLGDIPMNTWDIVFKP
EILSKLKDCGVYFLDGPEEVIPSAMKYLGLDPNSKNPDDIAKAGELLMSVRPYIKKFDSSGYINALARGDICLAVGWSGD
VFQAKTRAAEAAKKTKKPPVNVAYVIPKQGALMWFDNFAIPKDAKNIDNALVFINYMMRPEVAAKNSNFISYANGNLESQ
KFIDKDILNNPSIYPDKETFERLFIVTTNEPPVQRVITKTWNSFKRGK
>Mature_368_residues
MRRHFVAAIMCATLGWAGAASAQDKVLNVFNWSDYIDPTVLEDFTKETGIKVRYDVFDSNEVLETKLLAGKTGYDVVVPS
GSFLQRQIKAGIFLTLDKSKIPNLQYAWPEVTKRLAVYDPDNAHAVNYMWGTTGIGYNVDKVKERLGDIPMNTWDIVFKP
EILSKLKDCGVYFLDGPEEVIPSAMKYLGLDPNSKNPDDIAKAGELLMSVRPYIKKFDSSGYINALARGDICLAVGWSGD
VFQAKTRAAEAAKKTKKPPVNVAYVIPKQGALMWFDNFAIPKDAKNIDNALVFINYMMRPEVAAKNSNFISYANGNLESQ
KFIDKDILNNPSIYPDKETFERLFIVTTNEPPVQRVITKTWNSFKRGK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=364, Percent_Identity=51.9230769230769, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1787367, Length=325, Percent_Identity=34.7692307692308, Blast_Score=191, Evalue=7e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 41297; Mature: 41297

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRHFVAAIMCATLGWAGAASAQDKVLNVFNWSDYIDPTVLEDFTKETGIKVRYDVFDSN
CCHHHHHHHHHHHHCCCCCCCCHHHEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCC
EVLETKLLAGKTGYDVVVPSGSFLQRQIKAGIFLTLDKSKIPNLQYAWPEVTKRLAVYDP
HHHHHHHHCCCCCCEEEECCCHHHHHHHHCCEEEEECCCCCCCCEECCHHHHCEEEEECC
DNAHAVNYMWGTTGIGYNVDKVKERLGDIPMNTWDIVFKPEILSKLKDCGVYFLDGPEEV
CCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCEEEEECHHHHHHHHHCCEEEECCHHHH
IPSAMKYLGLDPNSKNPDDIAKAGELLMSVRPYIKKFDSSGYINALARGDICLAVGWSGD
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCCC
VFQAKTRAAEAAKKTKKPPVNVAYVIPKQGALMWFDNFAIPKDAKNIDNALVFINYMMRP
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCCHHHCCEEEEEEHHHCC
EVAAKNSNFISYANGNLESQKFIDKDILNNPSIYPDKETFERLFIVTTNEPPVQRVITKT
CHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHCCEEEEEECCCCHHHHHHHHH
WNSFKRGK
HHHHCCCC
>Mature Secondary Structure
MRRHFVAAIMCATLGWAGAASAQDKVLNVFNWSDYIDPTVLEDFTKETGIKVRYDVFDSN
CCHHHHHHHHHHHHCCCCCCCCHHHEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCC
EVLETKLLAGKTGYDVVVPSGSFLQRQIKAGIFLTLDKSKIPNLQYAWPEVTKRLAVYDP
HHHHHHHHCCCCCCEEEECCCHHHHHHHHCCEEEEECCCCCCCCEECCHHHHCEEEEECC
DNAHAVNYMWGTTGIGYNVDKVKERLGDIPMNTWDIVFKPEILSKLKDCGVYFLDGPEEV
CCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCEEEEECHHHHHHHHHCCEEEECCHHHH
IPSAMKYLGLDPNSKNPDDIAKAGELLMSVRPYIKKFDSSGYINALARGDICLAVGWSGD
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCCC
VFQAKTRAAEAAKKTKKPPVNVAYVIPKQGALMWFDNFAIPKDAKNIDNALVFINYMMRP
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCCHHHCCEEEEEEHHHCC
EVAAKNSNFISYANGNLESQKFIDKDILNNPSIYPDKETFERLFIVTTNEPPVQRVITKT
CHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHCCEEEEEECCCCHHHHHHHHH
WNSFKRGK
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]