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The map label for this gene is cysP [H]

Identifier: 154246273

GI number: 154246273

Start: 2604290

End: 2605327

Strand: Direct

Name: cysP [H]

Synonym: Xaut_2331

Alternate gene names: 154246273

Gene position: 2604290-2605327 (Clockwise)

Preceding gene: 154246272

Following gene: 154246274

Centisome position: 49.05

GC content: 67.15

Gene sequence:

>1038_bases
ATGATCAGCCGCCGCGCCGTTCTTGCTCTCGCGCTCGCCGGCGCCGCCGCGGCGCCCCTCGGCACCCTGCCGGCCTTTGC
CCAGCAAGGACCGGCGCCACAGGGGCCGAACACGCTGCTCAATGTCTCGTACGACATCTCCCGCGAGCTGTTCACCGCGG
TGAACGCCGAGTTCATCCCCTTCTGGAAGGGCAAGACCGGCCAGGAGCTGACCGTGAACCAGAGCCATGCCGGCTCCTCG
CGCCAGGCCCGCGCCATCCTCGAGGGGCTGGAGGCGGACGTGGTCACCTTCAACCAGGTGATCGACGTGGACACCCTGGC
CAAGGCCGGCTTCGTGGCCAAGGACTGGCAGGCGAAGTTCCCGAACAACGCCTCGCCCTATTATTCCTTCCCCGCCTTCC
TGGTGCGCGCCGGCAATCCCAAGGGCATCAAGGACTGGTCGGACCTGGTGCGGGACGACGTGAAGGTCATCTTCCCCAAC
CCGAAGACCTCGGGCAATGCGCGCTACACCTATCTGGCCGCCTATGCCTTCGCGCTGGAGCAGTTCAAGGGCGATGCCGC
CAAGGCCGACGCCTTCGTCAAGAAGCTGCTTGCCAACGTGCCGGTGTTCGACACCGGCGGCCGTGCCGCCACCACCACCT
TCGTGGAGCGCGACACCGGCGACGTGCTGGTGACCTTCGAGGCCGAGACCCGCACCATCGAGGGCCTCTATCCCGACCGC
AAGCTGGAGACGGTGGTGCCTTCCGTGAGCCTGCTGGCCGAATTCCCGGTGGCGGTGGTGGACAAGGTGGTGGACAAGCG
CGGCTCCCGCGCCTCGGCCACGGCCTATCTTGAGTTCCTCTATTCCCCGGCCGGCCAGACCGTTGCCGCCAAGGCCTTCC
ACCGGGTGGTGGACCCCAAGGTGGCCGAGGCGTTCAAGACCCAGTTCCCGCCGGTGCGCCTGGTGAAGGTGGAGGACGTG
TTCGGCGGCTGGCAGAAGCTCCAGGCCGAGCATTTCGCCTCCGGGGGCAAGCTGGATTCGCTCTTTACCGGCAAGTGA

Upstream 100 bases:

>100_bases
CCTGCCGCAAGGGCGGTTCACATCCCAAATTTCACATGCTATGCATGCGTCATGTGAATTGTACATTTCACCGATTGTGA
AATGTGGATGGAGAAGCCTG

Downstream 100 bases:

>100_bases
GCGGGGAAGAGTGAGCAGAGCGGGAGGAGCCCCGGTCGCCCGGTGAGGCGCGCGCGCCCCGGTGACATTCCGAGAGAGGA
GAAGGCGCCTTACGGCGCCT

Product: sulfate ABC transporter periplasmic sulfate-binding protein

Products: SO42- [Cytoplasm]; phosphate; ADP; S2O32- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MISRRAVLALALAGAAAAPLGTLPAFAQQGPAPQGPNTLLNVSYDISRELFTAVNAEFIPFWKGKTGQELTVNQSHAGSS
RQARAILEGLEADVVTFNQVIDVDTLAKAGFVAKDWQAKFPNNASPYYSFPAFLVRAGNPKGIKDWSDLVRDDVKVIFPN
PKTSGNARYTYLAAYAFALEQFKGDAAKADAFVKKLLANVPVFDTGGRAATTTFVERDTGDVLVTFEAETRTIEGLYPDR
KLETVVPSVSLLAEFPVAVVDKVVDKRGSRASATAYLEFLYSPAGQTVAAKAFHRVVDPKVAEAFKTQFPPVRLVKVEDV
FGGWQKLQAEHFASGGKLDSLFTGK

Sequences:

>Translated_345_residues
MISRRAVLALALAGAAAAPLGTLPAFAQQGPAPQGPNTLLNVSYDISRELFTAVNAEFIPFWKGKTGQELTVNQSHAGSS
RQARAILEGLEADVVTFNQVIDVDTLAKAGFVAKDWQAKFPNNASPYYSFPAFLVRAGNPKGIKDWSDLVRDDVKVIFPN
PKTSGNARYTYLAAYAFALEQFKGDAAKADAFVKKLLANVPVFDTGGRAATTTFVERDTGDVLVTFEAETRTIEGLYPDR
KLETVVPSVSLLAEFPVAVVDKVVDKRGSRASATAYLEFLYSPAGQTVAAKAFHRVVDPKVAEAFKTQFPPVRLVKVEDV
FGGWQKLQAEHFASGGKLDSLFTGK
>Mature_345_residues
MISRRAVLALALAGAAAAPLGTLPAFAQQGPAPQGPNTLLNVSYDISRELFTAVNAEFIPFWKGKTGQELTVNQSHAGSS
RQARAILEGLEADVVTFNQVIDVDTLAKAGFVAKDWQAKFPNNASPYYSFPAFLVRAGNPKGIKDWSDLVRDDVKVIFPN
PKTSGNARYTYLAAYAFALEQFKGDAAKADAFVKKLLANVPVFDTGGRAATTTFVERDTGDVLVTFEAETRTIEGLYPDR
KLETVVPSVSLLAEFPVAVVDKVVDKRGSRASATAYLEFLYSPAGQTVAAKAFHRVVDPKVAEAFKTQFPPVRLVKVEDV
FGGWQKLQAEHFASGGKLDSLFTGK

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. This protein specifically binds thiosulfate and is involved in its transmembrane transport [H]

COG id: COG4150

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1788765, Length=306, Percent_Identity=55.2287581699346, Blast_Score=344, Evalue=6e-96,
Organism=Escherichia coli, GI1790351, Length=303, Percent_Identity=48.8448844884488, Blast_Score=308, Evalue=4e-85,

Paralogues:

None

Copy number: 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37269; Mature: 37269

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS00401 PROK_SULFATE_BIND_1 ; PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISRRAVLALALAGAAAAPLGTLPAFAQQGPAPQGPNTLLNVSYDISRELFTAVNAEFIP
CCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHCCCEEE
FWKGKTGQELTVNQSHAGSSRQARAILEGLEADVVTFNQVIDVDTLAKAGFVAKDWQAKF
EECCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEHHHHHCHHHHHHCCCEECCCCCCC
PNNASPYYSFPAFLVRAGNPKGIKDWSDLVRDDVKVIFPNPKTSGNARYTYLAAYAFALE
CCCCCCCEECCEEEEECCCCCCCHHHHHHHHCCCEEEECCCCCCCCCEEHHHHHHHHHHH
QFKGDAAKADAFVKKLLANVPVFDTGGRAATTTFVERDTGDVLVTFEAETRTIEGLYPDR
HHCCCHHHHHHHHHHHHHCCCEEECCCCEEEEEEEECCCCCEEEEEECCCCEEECCCCCC
KLETVVPSVSLLAEFPVAVVDKVVDKRGSRASATAYLEFLYSPAGQTVAAKAFHRVVDPK
HHHHHHCHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCHH
VAEAFKTQFPPVRLVKVEDVFGGWQKLQAEHFASGGKLDSLFTGK
HHHHHHHCCCCEEEEEEHHHHCCHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MISRRAVLALALAGAAAAPLGTLPAFAQQGPAPQGPNTLLNVSYDISRELFTAVNAEFIP
CCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHCCCEEE
FWKGKTGQELTVNQSHAGSSRQARAILEGLEADVVTFNQVIDVDTLAKAGFVAKDWQAKF
EECCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEHHHHHCHHHHHHCCCEECCCCCCC
PNNASPYYSFPAFLVRAGNPKGIKDWSDLVRDDVKVIFPNPKTSGNARYTYLAAYAFALE
CCCCCCCEECCEEEEECCCCCCCHHHHHHHHCCCEEEECCCCCCCCCEEHHHHHHHHHHH
QFKGDAAKADAFVKKLLANVPVFDTGGRAATTTFVERDTGDVLVTFEAETRTIEGLYPDR
HHCCCHHHHHHHHHHHHHCCCEEECCCCEEEEEEEECCCCCEEEEEECCCCEEECCCCCC
KLETVVPSVSLLAEFPVAVVDKVVDKRGSRASATAYLEFLYSPAGQTVAAKAFHRVVDPK
HHHHHHCHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCHH
VAEAFKTQFPPVRLVKVEDVFGGWQKLQAEHFASGGKLDSLFTGK
HHHHHHHCCCCEEEEEEHHHHCCHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: SO42- [Periplasm]; H2O; ATP; S2O32- [Periplasm] [C]

Specific reaction: SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2188959; 9205837; 9278503; 9298646 [H]