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The map label for this gene is hisF

Identifier: 154246231

GI number: 154246231

Start: 2554692

End: 2555468

Strand: Reverse

Name: hisF

Synonym: Xaut_2289

Alternate gene names: 154246231

Gene position: 2555468-2554692 (Counterclockwise)

Preceding gene: 154246232

Following gene: 154246230

Centisome position: 48.14

GC content: 69.24

Gene sequence:

>777_bases
ATGCTGAAAGTCCGCGTCATCCCCTGCCTCGACGTGAAGGACGGCCGCGTGGTGAAGGGTGTCCAGTTCGTGGACCTGCG
CGACGCCGGCGATCCGGTGGAGGCGGCACGCGCCTATGACCTCGCCGGCGCGGACGAGTTGACCTTCCTCGACATCACCG
CCAGCCACGAGAACCGCGGCACCATCCTCGACGTGGTGCGCCGCACCGCCGAGCAGTGCTTCATGCCGCTGACCGTTGGC
GGCGGCGTGCGCACCGTGGACGACGTGCGCACCCTGCTCCATGCCGGCGCCGACAAGGTCTCCATCAATACCGCCGCCGT
GAACCGCCGCGCCTTCGTGGGCGAGGCGGCCGAGAAGTTCGGCGAGCAGTGCATCGTGGTGGCCATCGACGCCAAGCGCG
TGAGCAAGGAGGGCGAGGAGAACCGCTGGGAAATCTTTACCCATGGCGGGCGCACCCCTACCGGCATCGACGCCATCCAG
TTCGCCCGCGAGGTGGTGGACCTCGGCGCCGGCGAAATCCTGCTGACATCCATGGACCGGGACGGCACCGGCCAGGGCTT
CGACTGCGCCCTCACCCGCGCGGTGGCGGACGCTGTGCATGTGCCGGTGATCGCCTCCGGCGGCGTCGGCACCCTCGACC
ATCTGGTGGACGGCATCCGCGAAGGCGGTGCCTCGGCGGTGCTGGCCGCCTCCATCTTCCATTTCGGCACCTTCACCATC
CGCGAGGCCAAGGAACGCCTTGCGGAAGCGGGGCTGCCTGTCCGCCTCGACGTCTAG

Upstream 100 bases:

>100_bases
GCGAAAAAGCTGGAAGGCGCCATTACCGGCCGCGCCCTCTATGATGGCCGGCTCGATGCCCGCGAGGCGCTGGCGCTGGT
GGCGGGACGATAGGTGTGCC

Downstream 100 bases:

>100_bases
GCCGCGCGCCTTAACGCGTCCTCCCCGCCCTCCCTTTCCGGGCCACCTTGATGAGCGACAGCTTCACCCTCGCCGACCTC
GAAGCCATCGTCGCGGCGCG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLKVRVIPCLDVKDGRVVKGVQFVDLRDAGDPVEAARAYDLAGADELTFLDITASHENRGTILDVVRRTAEQCFMPLTVG
GGVRTVDDVRTLLHAGADKVSINTAAVNRRAFVGEAAEKFGEQCIVVAIDAKRVSKEGEENRWEIFTHGGRTPTGIDAIQ
FAREVVDLGAGEILLTSMDRDGTGQGFDCALTRAVADAVHVPVIASGGVGTLDHLVDGIREGGASAVLAASIFHFGTFTI
REAKERLAEAGLPVRLDV

Sequences:

>Translated_258_residues
MLKVRVIPCLDVKDGRVVKGVQFVDLRDAGDPVEAARAYDLAGADELTFLDITASHENRGTILDVVRRTAEQCFMPLTVG
GGVRTVDDVRTLLHAGADKVSINTAAVNRRAFVGEAAEKFGEQCIVVAIDAKRVSKEGEENRWEIFTHGGRTPTGIDAIQ
FAREVVDLGAGEILLTSMDRDGTGQGFDCALTRAVADAVHVPVIASGGVGTLDHLVDGIREGGASAVLAASIFHFGTFTI
REAKERLAEAGLPVRLDV
>Mature_258_residues
MLKVRVIPCLDVKDGRVVKGVQFVDLRDAGDPVEAARAYDLAGADELTFLDITASHENRGTILDVVRRTAEQCFMPLTVG
GGVRTVDDVRTLLHAGADKVSINTAAVNRRAFVGEAAEKFGEQCIVVAIDAKRVSKEGEENRWEIFTHGGRTPTGIDAIQ
FAREVVDLGAGEILLTSMDRDGTGQGFDCALTRAVADAVHVPVIASGGVGTLDHLVDGIREGGASAVLAASIFHFGTFTI
REAKERLAEAGLPVRLDV

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=41.2451361867704, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI87082028, Length=242, Percent_Identity=30.5785123966942, Blast_Score=99, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=35.2380952380952, Blast_Score=158, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_XANP2 (A7IHN9)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001417189.1
- ProteinModelPortal:   A7IHN9
- SMR:   A7IHN9
- STRING:   A7IHN9
- GeneID:   5420797
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_2289
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   XAUT78245:XAUT_2289-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27448; Mature: 27448

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVRVIPCLDVKDGRVVKGVQFVDLRDAGDPVEAARAYDLAGADELTFLDITASHENRG
CEEEEEEEEECCCCCCEECCEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCC
TILDVVRRTAEQCFMPLTVGGGVRTVDDVRTLLHAGADKVSINTAAVNRRAFVGEAAEKF
HHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHCCCCEEEEEHHHHCCHHHHHHHHHHC
GEQCIVVAIDAKRVSKEGEENRWEIFTHGGRTPTGIDAIQFAREVVDLGAGEILLTSMDR
CCEEEEEEEEHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCC
DGTGQGFDCALTRAVADAVHVPVIASGGVGTLDHLVDGIREGGASAVLAASIFHFGTFTI
CCCCCCHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCHHH
REAKERLAEAGLPVRLDV
HHHHHHHHHCCCCEEECC
>Mature Secondary Structure
MLKVRVIPCLDVKDGRVVKGVQFVDLRDAGDPVEAARAYDLAGADELTFLDITASHENRG
CEEEEEEEEECCCCCCEECCEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCC
TILDVVRRTAEQCFMPLTVGGGVRTVDDVRTLLHAGADKVSINTAAVNRRAFVGEAAEKF
HHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHCCCCEEEEEHHHHCCHHHHHHHHHHC
GEQCIVVAIDAKRVSKEGEENRWEIFTHGGRTPTGIDAIQFAREVVDLGAGEILLTSMDR
CCEEEEEEEEHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCC
DGTGQGFDCALTRAVADAVHVPVIASGGVGTLDHLVDGIREGGASAVLAASIFHFGTFTI
CCCCCCHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCHHH
REAKERLAEAGLPVRLDV
HHHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA