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The map label for this gene is hisA

Identifier: 153936234

GI number: 153936234

Start: 1672354

End: 1673082

Strand: Direct

Name: hisA

Synonym: CLC_1601

Alternate gene names: 153936234

Gene position: 1672354-1673082 (Clockwise)

Preceding gene: 153935259

Following gene: 153936729

Centisome position: 44.47

GC content: 29.63

Gene sequence:

>729_bases
ATGATAATTTTACCTGCCATAGATTTAAAAGAGGGTAAATGTATTAGACTATACCAAGGAGATTTTAAAGCTTCAAAGGT
GGTAGCAGAAGATCCTATAGAGGTAGCACTAAAGTTTAAAGAAAATGGAGCAGAATATATACATATAGTGGATTTAGACG
GAGCTCTTACTGGAGAAATTAAAAATTTATCTATAATTTCTTCCATAATAAAAACAATTAATATACCTGTAGAGCTTGGA
GGTGGAATAAGAAATTTAAATACAATAGATATGCTTATAGGCGCAGGAATTGAAAGGGTAATATTAGGTACAGCTGCATT
AAATAATAGAGGACTTGTTGAAGAGGCTGTGAAAAAATATGATGAAAAAATTGCCATAGGAATAGATGCTAAAAATGAAA
AGGTAGCTATAAATGGATGGTTAAATGTAAGTAGTATAAACTATATAGATTTTGCTAAAGAAATGGAAAAGATAGGAGTT
AGAAATATTATATTCACGGATATTAGTAAAGATGGAACTTTAAAGGGGCCTAATTTAAAACAGCTTGAAAAACTAAATGA
GAGTATAAATTGTAATGTTATAGCCTCAGGAGGGATTAAAGATATAGAGGATTTAAAGGGTATAAAAGAAATGGATGTTT
ATGGAGCTATTGTAGGTAAAGCTATATATTCAGGGAATATTAATTTGAATGAAGCTATTAAAATTATAAACAAGGGGAGT
TCTAAATGA

Upstream 100 bases:

>100_bases
GGATAGTAAGCAAAAGTAATATTTATGGAATACAGTTTCATCCTGAAAAAAGTGGGGATATAGGACTTAAAATATTAAGT
AATTTTGGGGAGATGATAAA

Downstream 100 bases:

>100_bases
GTAAATATTGGAGCAATATAACTAAAGATATAGAACCTTATGTATGTGGAGAGCAACCTAAGAATAAAAAAATTATAAAG
TTAAATACTAATGAAAATCC

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MIILPAIDLKEGKCIRLYQGDFKASKVVAEDPIEVALKFKENGAEYIHIVDLDGALTGEIKNLSIISSIIKTINIPVELG
GGIRNLNTIDMLIGAGIERVILGTAALNNRGLVEEAVKKYDEKIAIGIDAKNEKVAINGWLNVSSINYIDFAKEMEKIGV
RNIIFTDISKDGTLKGPNLKQLEKLNESINCNVIASGGIKDIEDLKGIKEMDVYGAIVGKAIYSGNINLNEAIKIINKGS
SK

Sequences:

>Translated_242_residues
MIILPAIDLKEGKCIRLYQGDFKASKVVAEDPIEVALKFKENGAEYIHIVDLDGALTGEIKNLSIISSIIKTINIPVELG
GGIRNLNTIDMLIGAGIERVILGTAALNNRGLVEEAVKKYDEKIAIGIDAKNEKVAINGWLNVSSINYIDFAKEMEKIGV
RNIIFTDISKDGTLKGPNLKQLEKLNESINCNVIASGGIKDIEDLKGIKEMDVYGAIVGKAIYSGNINLNEAIKIINKGS
SK
>Mature_242_residues
MIILPAIDLKEGKCIRLYQGDFKASKVVAEDPIEVALKFKENGAEYIHIVDLDGALTGEIKNLSIISSIIKTINIPVELG
GGIRNLNTIDMLIGAGIERVILGTAALNNRGLVEEAVKKYDEKIAIGIDAKNEKVAINGWLNVSSINYIDFAKEMEKIGV
RNIIFTDISKDGTLKGPNLKQLEKLNESINCNVIASGGIKDIEDLKGIKEMDVYGAIVGKAIYSGNINLNEAIKIINKGS
SK

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=239, Percent_Identity=30.9623430962343, Blast_Score=148, Evalue=3e-37,
Organism=Escherichia coli, GI1788336, Length=241, Percent_Identity=25.3112033195021, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_CLOB1 (A7FU80)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001383913.1
- ProteinModelPortal:   A7FU80
- SMR:   A7FU80
- STRING:   A7FU80
- GeneID:   5394758
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_1590
- eggNOG:   COG0106
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   PRK00748
- BioCyc:   CBOT441770:CLB_1590-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26223; Mature: 26223

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIILPAIDLKEGKCIRLYQGDFKASKVVAEDPIEVALKFKENGAEYIHIVDLDGALTGEI
CEEECCCCCCCCCEEEEEECCCCCCEEEECCCEEEEEEEECCCCCEEEEEEECCCCCCCC
KNLSIISSIIKTINIPVELGGGIRNLNTIDMLIGAGIERVILGTAALNNRGLVEEAVKKY
CHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHCCHHHEEEEHHHCCCCCHHHHHHHHH
DEKIAIGIDAKNEKVAINGWLNVSSINYIDFAKEMEKIGVRNIIFTDISKDGTLKGPNLK
CCEEEEEECCCCCEEEEEEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHH
QLEKLNESINCNVIASGGIKDIEDLKGIKEMDVYGAIVGKAIYSGNINLNEAIKIINKGS
HHHHHCCCCCEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHEEECCCCHHHHHHEECCCC
SK
CC
>Mature Secondary Structure
MIILPAIDLKEGKCIRLYQGDFKASKVVAEDPIEVALKFKENGAEYIHIVDLDGALTGEI
CEEECCCCCCCCCEEEEEECCCCCCEEEECCCEEEEEEEECCCCCEEEEEEECCCCCCCC
KNLSIISSIIKTINIPVELGGGIRNLNTIDMLIGAGIERVILGTAALNNRGLVEEAVKKY
CHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHCCHHHEEEEHHHCCCCCHHHHHHHHH
DEKIAIGIDAKNEKVAINGWLNVSSINYIDFAKEMEKIGVRNIIFTDISKDGTLKGPNLK
CCEEEEEECCCCCEEEEEEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHH
QLEKLNESINCNVIASGGIKDIEDLKGIKEMDVYGAIVGKAIYSGNINLNEAIKIINKGS
HHHHHCCCCCEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHEEECCCCHHHHHHEECCCC
SK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA