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The map label for this gene is hisH [H]

Identifier: 153935259

GI number: 153935259

Start: 1671752

End: 1672357

Strand: Direct

Name: hisH [H]

Synonym: CLC_1600

Alternate gene names: 153935259

Gene position: 1671752-1672357 (Clockwise)

Preceding gene: 153935645

Following gene: 153936234

Centisome position: 44.45

GC content: 28.88

Gene sequence:

>606_bases
GTGATTTCAATTGTGGATTATGGTATGGGAAATTTAAAAAGTGTAGAAAATGCACTTAATTTTTTAGGTATAAAGTCTGT
AATTACATCAGAGAGAGAAGTAATTTTAAATAGTGATGGAATAATATTACCAGGAGTGGGAGCTTTTCCAGATGCCATTA
ATAACATTAAAAGAGATGGTATTGATAAAGTTTTAAAGGAAGCAGTAAAAAAGGATAAACCTTTACTTGGAATATGTCTT
GGAATGCAGCTTTTATTTGAAGAAAGTGAAGAAATTAAAAGCTGTAGAGGATTAGGTTTTTTAAAAGGAAAAATAGAAAA
AATGAAGGTAAATTTAAAAATACCTCATATGGGTTGGAATAATTTAAGTTTTTGTAAAGATTCCCCAATACTTAATGGTA
TAAAAGAGAACAGTTATGTGTATTATGTTCACTCTTATTGTGCCAAAATTGAAGAGGAAGGAATATTAAATACCTATTCC
CAATATGAAATAGAAGTACCGGGGATAGTAAGCAAAAGTAATATTTATGGAATACAGTTTCATCCTGAAAAAAGTGGGGA
TATAGGACTTAAAATATTAAGTAATTTTGGGGAGATGATAAAATGA

Upstream 100 bases:

>100_bases
ACAATTATGTTTATTATTAAGTATTACGAGCTTTACAAGGAGTAATAGGGAATATATAAAGCCGATAACATTAAAAAAGA
AATATAAGGGGGACAAATAG

Downstream 100 bases:

>100_bases
TAATTTTACCTGCCATAGATTTAAAAGAGGGTAAATGTATTAGACTATACCAAGGAGATTTTAAAGCTTCAAAGGTGGTA
GCAGAAGATCCTATAGAGGT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MISIVDYGMGNLKSVENALNFLGIKSVITSEREVILNSDGIILPGVGAFPDAINNIKRDGIDKVLKEAVKKDKPLLGICL
GMQLLFEESEEIKSCRGLGFLKGKIEKMKVNLKIPHMGWNNLSFCKDSPILNGIKENSYVYYVHSYCAKIEEEGILNTYS
QYEIEVPGIVSKSNIYGIQFHPEKSGDIGLKILSNFGEMIK

Sequences:

>Translated_201_residues
MISIVDYGMGNLKSVENALNFLGIKSVITSEREVILNSDGIILPGVGAFPDAINNIKRDGIDKVLKEAVKKDKPLLGICL
GMQLLFEESEEIKSCRGLGFLKGKIEKMKVNLKIPHMGWNNLSFCKDSPILNGIKENSYVYYVHSYCAKIEEEGILNTYS
QYEIEVPGIVSKSNIYGIQFHPEKSGDIGLKILSNFGEMIK
>Mature_201_residues
MISIVDYGMGNLKSVENALNFLGIKSVITSEREVILNSDGIILPGVGAFPDAINNIKRDGIDKVLKEAVKKDKPLLGICL
GMQLLFEESEEIKSCRGLGFLKGKIEKMKVNLKIPHMGWNNLSFCKDSPILNGIKENSYVYYVHSYCAKIEEEGILNTYS
QYEIEVPGIVSKSNIYGIQFHPEKSGDIGLKILSNFGEMIK

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=199, Percent_Identity=36.6834170854271, Blast_Score=132, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6319725, Length=208, Percent_Identity=32.2115384615385, Blast_Score=109, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22349; Mature: 22349

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISIVDYGMGNLKSVENALNFLGIKSVITSEREVILNSDGIILPGVGAFPDAINNIKRDG
CEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECCCCCCHHHHHHHHHCC
IDKVLKEAVKKDKPLLGICLGMQLLFEESEEIKSCRGLGFLKGKIEKMKVNLKIPHMGWN
HHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHCEEEEEEEEEEECCCCCC
NLSFCKDSPILNGIKENSYVYYVHSYCAKIEEEGILNTYSQYEIEVPGIVSKSNIYGIQF
CCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCEEEEECCCEEECCCEEEEEE
HPEKSGDIGLKILSNFGEMIK
CCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MISIVDYGMGNLKSVENALNFLGIKSVITSEREVILNSDGIILPGVGAFPDAINNIKRDG
CEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECCCCCCHHHHHHHHHCC
IDKVLKEAVKKDKPLLGICLGMQLLFEESEEIKSCRGLGFLKGKIEKMKVNLKIPHMGWN
HHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHCEEEEEEEEEEECCCCCC
NLSFCKDSPILNGIKENSYVYYVHSYCAKIEEEGILNTYSQYEIEVPGIVSKSNIYGIQF
CCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCEEEEECCCEEECCCEEEEEE
HPEKSGDIGLKILSNFGEMIK
CCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]