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The map label for this gene is tig

Identifier: 152976888

GI number: 152976888

Start: 3241676

End: 3242959

Strand: Reverse

Name: tig

Synonym: Bcer98_3187

Alternate gene names: 152976888

Gene position: 3242959-3241676 (Counterclockwise)

Preceding gene: 152976889

Following gene: 152976887

Centisome position: 79.35

GC content: 38.63

Gene sequence:

>1284_bases
ATGGCTGCAAAATGGGAAAAATTAGAAGGTAACGTTGGCGTATTAACAATCGAAGTTGATGCTAAAGAAGTTAACAAATC
TCTTGACGCTGCGTTCAAAAAAGTAGTAAAAACAATTAACGTACCAGGTTTCCGTAAAGGTAAAATGCCTCGTCCGTTAT
TCGAACAACGCTTTGGTGTAGAATCTTTATACCAAGATGCATTAGACATCATCTTACCAAAAGCATACAGTGAAGCAATC
GATGAAACTGGTATCTTCCCAGTTGCTCATCCTGAGATTGACATCGAGAAGTTCGAAAAAAATGAAAACCTTGTTTTCAC
TGCGAAAGTTACTGTAAAACCTGAAGTGAAACTAGGCGAGTATAAAGGTTTAGCAGTAGAAAAAATCGACACAACTGTAA
CTGACGAAGATGTAGAGAACGAATTAAAAGCTTTACAAGAGCGTCAAGCTGAACTAGTTGTGAAAGAAGATGGAACTGTT
GAAAACGGTGATACAGCTGTAATCGACTTTGAAGGTTTCGTGAATGGCGAAGCATTCGAAGGTGGAAAAGGTGAAAACTA
CTCTCTAGCAATCGGTTCTGGTACATTCATCCCAGGCTTTGAAGAGCAATTAATTGGTCTTAAAGCAGGTGAGTCTAAAG
ATGTTGAAGTAACATTCCCAGAAGAGTACCATGCAGAAGAACTTGCTGGAAAACCAGCAACATTCAAAGTAACAGTTCAT
GAAATCAAAGCAAAAGAACTTCCTGAGTTAAACGACGAGTTCGCTAAAGATGTGAACGAAGAAGTTGCAACTCTTGATGA
GTTAAAAGCAAAACTTCGCACAGACTTAGAAGAAGGCAAAAAGCATGAAGCTGAACACAAAGTGCGTGACGAAGTGGTAG
AAAAAGCTGCTGCTAACGCTGAAATCGACATTCCAGAAGCTATGATCGAAACAGAATTAGATCGTATGGTTCGTGAATTT
GAACAACGTTTAAGCCAACAAGGTATGAACCTTGAGCTTTACTACCAATTCACAGGTACTGATGCTGACAAGCTAAAAGA
ACAAATGAAAGAAGATGCGCAAAAACGTGTAAGAATCAACCTTGTTCTTGAAGCAATCATTAAAGCTGAAAACATCGAAG
TTGCTGAAGAAGAAGTAAATGCAGAAATTGAAAAAATGGCTGAAATGTACAATATGCCAGTAGATGCTATTAAGCAAGCT
CTTGGAAGCGCAGAAGCTCTAGCTGAAGATCTTAAAGTTCGTAAAGCTGTAGACTTCTTAGTAGACAATAGCAAAGCTGC
ATAA

Upstream 100 bases:

>100_bases
ATTGGGTGTAGCGCTCTCGTTGTTTCTAAAGAAAGGAATAATGGAATTTTAATCTATAGAGGTATGTGCATACATTATTA
TAGTTGGAGGGAAAGAATAA

Downstream 100 bases:

>100_bases
TAAAATAACAAGGCGCGATTTTAATCGTGCCTTGTTTTATAAATATGAATATATTTGTAAAACTTTTTGGTGAGGAAGGA
AAAGAACCTTTAAAAGTCGA

Product: trigger factor

Products: peptidylproline (omega=0)

Alternate protein names: TF

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MAAKWEKLEGNVGVLTIEVDAKEVNKSLDAAFKKVVKTINVPGFRKGKMPRPLFEQRFGVESLYQDALDIILPKAYSEAI
DETGIFPVAHPEIDIEKFEKNENLVFTAKVTVKPEVKLGEYKGLAVEKIDTTVTDEDVENELKALQERQAELVVKEDGTV
ENGDTAVIDFEGFVNGEAFEGGKGENYSLAIGSGTFIPGFEEQLIGLKAGESKDVEVTFPEEYHAEELAGKPATFKVTVH
EIKAKELPELNDEFAKDVNEEVATLDELKAKLRTDLEEGKKHEAEHKVRDEVVEKAAANAEIDIPEAMIETELDRMVREF
EQRLSQQGMNLELYYQFTGTDADKLKEQMKEDAQKRVRINLVLEAIIKAENIEVAEEEVNAEIEKMAEMYNMPVDAIKQA
LGSAEALAEDLKVRKAVDFLVDNSKAA

Sequences:

>Translated_427_residues
MAAKWEKLEGNVGVLTIEVDAKEVNKSLDAAFKKVVKTINVPGFRKGKMPRPLFEQRFGVESLYQDALDIILPKAYSEAI
DETGIFPVAHPEIDIEKFEKNENLVFTAKVTVKPEVKLGEYKGLAVEKIDTTVTDEDVENELKALQERQAELVVKEDGTV
ENGDTAVIDFEGFVNGEAFEGGKGENYSLAIGSGTFIPGFEEQLIGLKAGESKDVEVTFPEEYHAEELAGKPATFKVTVH
EIKAKELPELNDEFAKDVNEEVATLDELKAKLRTDLEEGKKHEAEHKVRDEVVEKAAANAEIDIPEAMIETELDRMVREF
EQRLSQQGMNLELYYQFTGTDADKLKEQMKEDAQKRVRINLVLEAIIKAENIEVAEEEVNAEIEKMAEMYNMPVDAIKQA
LGSAEALAEDLKVRKAVDFLVDNSKAA
>Mature_426_residues
AAKWEKLEGNVGVLTIEVDAKEVNKSLDAAFKKVVKTINVPGFRKGKMPRPLFEQRFGVESLYQDALDIILPKAYSEAID
ETGIFPVAHPEIDIEKFEKNENLVFTAKVTVKPEVKLGEYKGLAVEKIDTTVTDEDVENELKALQERQAELVVKEDGTVE
NGDTAVIDFEGFVNGEAFEGGKGENYSLAIGSGTFIPGFEEQLIGLKAGESKDVEVTFPEEYHAEELAGKPATFKVTVHE
IKAKELPELNDEFAKDVNEEVATLDELKAKLRTDLEEGKKHEAEHKVRDEVVEKAAANAEIDIPEAMIETELDRMVREFE
QRLSQQGMNLELYYQFTGTDADKLKEQMKEDAQKRVRINLVLEAIIKAENIEVAEEEVNAEIEKMAEMYNMPVDAIKQAL
GSAEALAEDLKVRKAVDFLVDNSKAA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=400, Percent_Identity=34.75, Blast_Score=223, Evalue=2e-59,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_BACCN (A7GTF2)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001376405.1
- ProteinModelPortal:   A7GTF2
- STRING:   A7GTF2
- EnsemblBacteria:   EBBACT00000039556
- GeneID:   5343544
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_3187
- eggNOG:   COG0544
- GeneTree:   EBGT00050000000344
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- BioCyc:   BCER315749:BCER98_3187-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: 5.2.1.8

Molecular weight: Translated: 47669; Mature: 47538

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKWEKLEGNVGVLTIEVDAKEVNKSLDAAFKKVVKTINVPGFRKGKMPRPLFEQRFGV
CCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCH
ESLYQDALDIILPKAYSEAIDETGIFPVAHPEIDIEKFEKNENLVFTAKVTVKPEVKLGE
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEEEEEEEEECCCCEECC
YKGLAVEKIDTTVTDEDVENELKALQERQAELVVKEDGTVENGDTAVIDFEGFVNGEAFE
CCCCEEEHHCCCCCCHHHHHHHHHHHHHHHCEEEECCCCCCCCCEEEEEECCCCCCCCCC
GGKGENYSLAIGSGTFIPGFEEQLIGLKAGESKDVEVTFPEEYHAEELAGKPATFKVTVH
CCCCCCEEEEECCCCCCCCHHHHHHCCCCCCCCCEEEECCHHHHHHHHCCCCCEEEEEHH
EIKAKELPELNDEFAKDVNEEVATLDELKAKLRTDLEEGKKHEAEHKVRDEVVEKAAANA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EIDIPEAMIETELDRMVREFEQRLSQQGMNLELYYQFTGTDADKLKEQMKEDAQKRVRIN
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHH
LVLEAIIKAENIEVAEEEVNAEIEKMAEMYNMPVDAIKQALGSAEALAEDLKVRKAVDFL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHH
VDNSKAA
HCCCCCC
>Mature Secondary Structure 
AAKWEKLEGNVGVLTIEVDAKEVNKSLDAAFKKVVKTINVPGFRKGKMPRPLFEQRFGV
CCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCH
ESLYQDALDIILPKAYSEAIDETGIFPVAHPEIDIEKFEKNENLVFTAKVTVKPEVKLGE
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEEEEEEEEECCCCEECC
YKGLAVEKIDTTVTDEDVENELKALQERQAELVVKEDGTVENGDTAVIDFEGFVNGEAFE
CCCCEEEHHCCCCCCHHHHHHHHHHHHHHHCEEEECCCCCCCCCEEEEEECCCCCCCCCC
GGKGENYSLAIGSGTFIPGFEEQLIGLKAGESKDVEVTFPEEYHAEELAGKPATFKVTVH
CCCCCCEEEEECCCCCCCCHHHHHHCCCCCCCCCEEEECCHHHHHHHHCCCCCEEEEEHH
EIKAKELPELNDEFAKDVNEEVATLDELKAKLRTDLEEGKKHEAEHKVRDEVVEKAAANA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EIDIPEAMIETELDRMVREFEQRLSQQGMNLELYYQFTGTDADKLKEQMKEDAQKRVRIN
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHH
LVLEAIIKAENIEVAEEEVNAEIEKMAEMYNMPVDAIKQALGSAEALAEDLKVRKAVDFL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHH
VDNSKAA
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (omega=180)

Specific reaction: peptidylproline (omega=180) = peptidylproline (omega=0)

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA