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The map label for this gene is dmlR [H]

Identifier: 152969572

GI number: 152969572

Start: 1165080

End: 1166021

Strand: Direct

Name: dmlR [H]

Synonym: KPN_01016

Alternate gene names: 152969572

Gene position: 1165080-1166021 (Clockwise)

Preceding gene: 152969564

Following gene: 152969577

Centisome position: 21.92

GC content: 61.46

Gene sequence:

>942_bases
ATGAATAATCTGCCGCTGCTAAATGATTTACGCGTCTTTATGCTGGTCGCTCGCCGGGCCGGGTTCGCCGCCGCCGCCGA
GGAGCTTGGCGTATCGCCGGCGTTCGTCAGCAAGCGGGTTTCGCTGCTGGAGCAAACCCTGAATGTGATGTTGCTGCACC
GGACCACCCGTCGGGTCACCATTACCGAAGAGGGGGAGCGGATCTATGAATGGGCGCAGCGCATTTTGCAGGATGTCGAT
GAGATGATGGATGAGCTTTCCGACGTGCGCCAGGTGCCGCAGGGAACGCTGCGTATTATCAGTAGCTTCGGCTTTGGCCG
TCGGGTTGTCGCCCCGGCGCTGTCGGCGCTGGCTCTCCAGTACCCGCAGCTGGAGCTGCGTTTCGATGTCCAGGACCGGC
TGGTGGATTTAGTCAATGAAGGGGTGGATCTCGATATTCGCGTCGGCGACGATATCGCACCGAATCTGATTGCCCGCCAG
CTGGCGGCCAATCACCGCGTACTCTGCGCCTCGCCGCAGTTCCTTGCCCGCCATGCGCCGCCGAAACAGCTGAGCGATCT
GGCCGCGTTACCCTGCCTGGTGATTAAAGAGCGCGATCATCCGTTCGGCGTCTGGCAATTGCATAGCAAAGAGGGCCAGC
ACGCCATCAAGGTAACCGGGCCGCTCTCCTCCAACCATGGTGAAATTGTGCATCAGTGGTGTCTGGACGGGCAGGGGATT
GCGCTCCGTTCGTGGTGGGACGTCCGGGAGAATATCGCCAGCGGACATCTGGTGCAGGTGCTGCCGGACTACTGGCAGCC
GGCCAACGTCTGGGCGGTTTACGTCTCCCGGCTGGCGACGTCGGCCAAAATCCGCACCACCGTGGAGTTTCTGCGCCACT
ACTTTCAGCTGCACTATCCGCAGCATGAGCCGACGGCCAGCGCCGTCGGCAGAGGCGATTAA

Upstream 100 bases:

>100_bases
GGTTCTCACTTTTTAACGATGATGTTTGACCACCTGCCTATCCTATAATTGACTGGTTCATAATTAATCACGCTAACTGG
TGAAACATAAAACACAGATC

Downstream 100 bases:

>100_bases
ACCCCAAGGCGGTCGCGCAGGCTGTAGTAGGCGGCGCCCATGGCGGTAAAAGGAATGCGCAGGCTGCGGCCGCCCGGGAA
CGGGTAGTGCGGCAGTTTGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNNLPLLNDLRVFMLVARRAGFAAAAEELGVSPAFVSKRVSLLEQTLNVMLLHRTTRRVTITEEGERIYEWAQRILQDVD
EMMDELSDVRQVPQGTLRIISSFGFGRRVVAPALSALALQYPQLELRFDVQDRLVDLVNEGVDLDIRVGDDIAPNLIARQ
LAANHRVLCASPQFLARHAPPKQLSDLAALPCLVIKERDHPFGVWQLHSKEGQHAIKVTGPLSSNHGEIVHQWCLDGQGI
ALRSWWDVRENIASGHLVQVLPDYWQPANVWAVYVSRLATSAKIRTTVEFLRHYFQLHYPQHEPTASAVGRGD

Sequences:

>Translated_313_residues
MNNLPLLNDLRVFMLVARRAGFAAAAEELGVSPAFVSKRVSLLEQTLNVMLLHRTTRRVTITEEGERIYEWAQRILQDVD
EMMDELSDVRQVPQGTLRIISSFGFGRRVVAPALSALALQYPQLELRFDVQDRLVDLVNEGVDLDIRVGDDIAPNLIARQ
LAANHRVLCASPQFLARHAPPKQLSDLAALPCLVIKERDHPFGVWQLHSKEGQHAIKVTGPLSSNHGEIVHQWCLDGQGI
ALRSWWDVRENIASGHLVQVLPDYWQPANVWAVYVSRLATSAKIRTTVEFLRHYFQLHYPQHEPTASAVGRGD
>Mature_313_residues
MNNLPLLNDLRVFMLVARRAGFAAAAEELGVSPAFVSKRVSLLEQTLNVMLLHRTTRRVTITEEGERIYEWAQRILQDVD
EMMDELSDVRQVPQGTLRIISSFGFGRRVVAPALSALALQYPQLELRFDVQDRLVDLVNEGVDLDIRVGDDIAPNLIARQ
LAANHRVLCASPQFLARHAPPKQLSDLAALPCLVIKERDHPFGVWQLHSKEGQHAIKVTGPLSSNHGEIVHQWCLDGQGI
ALRSWWDVRENIASGHLVQVLPDYWQPANVWAVYVSRLATSAKIRTTVEFLRHYFQLHYPQHEPTASAVGRGD

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=301, Percent_Identity=87.7076411960133, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI1789440, Length=296, Percent_Identity=46.6216216216216, Blast_Score=284, Evalue=5e-78,
Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=34.3642611683849, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1787128, Length=302, Percent_Identity=28.8079470198675, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=31.1643835616438, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1789639, Length=291, Percent_Identity=30.5841924398625, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1787589, Length=269, Percent_Identity=27.1375464684015, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789173, Length=270, Percent_Identity=31.4814814814815, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1788706, Length=265, Percent_Identity=29.4339622641509, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786448, Length=308, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1786508, Length=268, Percent_Identity=25, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI157672245, Length=149, Percent_Identity=28.8590604026846, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1786713, Length=146, Percent_Identity=34.2465753424658, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35276; Mature: 35276

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLPLLNDLRVFMLVARRAGFAAAAEELGVSPAFVSKRVSLLEQTLNVMLLHRTTRRVT
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEECCCEEE
ITEEGERIYEWAQRILQDVDEMMDELSDVRQVPQGTLRIISSFGFGRRVVAPALSALALQ
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
YPQLELRFDVQDRLVDLVNEGVDLDIRVGDDIAPNLIARQLAANHRVLCASPQFLARHAP
CCCEEEEECHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCEEECCHHHHHHCCC
PKQLSDLAALPCLVIKERDHPFGVWQLHSKEGQHAIKVTGPLSSNHGEIVHQWCLDGQGI
HHHHHHHHHCCEEEEECCCCCCCEEEEECCCCCEEEEEECCCCCCCCHHHHHHHCCCCCE
ALRSWWDVRENIASGHLVQVLPDYWQPANVWAVYVSRLATSAKIRTTVEFLRHYFQLHYP
EEHHHHHHHHHHCCCCHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QHEPTASAVGRGD
CCCCCCCCCCCCC
>Mature Secondary Structure
MNNLPLLNDLRVFMLVARRAGFAAAAEELGVSPAFVSKRVSLLEQTLNVMLLHRTTRRVT
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEECCCEEE
ITEEGERIYEWAQRILQDVDEMMDELSDVRQVPQGTLRIISSFGFGRRVVAPALSALALQ
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
YPQLELRFDVQDRLVDLVNEGVDLDIRVGDDIAPNLIARQLAANHRVLCASPQFLARHAP
CCCEEEEECHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCEEECCHHHHHHCCC
PKQLSDLAALPCLVIKERDHPFGVWQLHSKEGQHAIKVTGPLSSNHGEIVHQWCLDGQGI
HHHHHHHHHCCEEEEECCCCCCCEEEEECCCCCEEEEEECCCCCCCCHHHHHHHCCCCCE
ALRSWWDVRENIASGHLVQVLPDYWQPANVWAVYVSRLATSAKIRTTVEFLRHYFQLHYP
EEHHHHHHHHHHCCCCHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QHEPTASAVGRGD
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]