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The map label for this gene is puuA [H]

Identifier: 152969577

GI number: 152969577

Start: 1170670

End: 1172091

Strand: Direct

Name: puuA [H]

Synonym: KPN_01021

Alternate gene names: 152969577

Gene position: 1170670-1172091 (Clockwise)

Preceding gene: 152969572

Following gene: 152969578

Centisome position: 22.03

GC content: 58.23

Gene sequence:

>1422_bases
ATGGAAACCAATATCGTAGAAGTTGAGAATTTTGTTCAGCAAACGGAAGAGAGACGGGTTAGCGCGTTCGCGAAAGAAGT
GAAGCGCTACCTCGAAACCTACCCTGATACCCAGTATGTCGATGTCCTCCTCACTGACCTGAACGGCTGCTTCCGCGGCA
AGCGCATCCCGGTTGCCGGCCTGAGCAAACTGGAGAAAGGCTGTTATTTCCCGGCCTCGGTGTTTGCGATGGATATTCTC
GGCAATGTGGTGGAAGAGGCTGGTCTGGGCCAGGAGATGGGCGAGCCGGATCGCAGCTGCATCCCGGTACCCGGCACCTT
AACCCCTTCGGCGGCTGACCCACAATCGATAGCCCAGGTACAGCTAACCATGGTTGATGAAGATGGCGCTCCCTTTGACG
TTGAGCCGCGGAACGTACTCAACCGCCTCTGGCAGCAGTTGCGCCAGCGCGGGCTGTTCCCCGTGGTAGCGGTAGAGCTG
GAGTTCTATTTACTGGACCGCAAACGCGATGCCGAAGGCTACCTGCAGCCGCCGTGTGCGCCGGGCACCGACGATCGCAA
CACCCAAAGCCAGGTCTACTCGGTCGATAACCTTAACCACTTCGCCGACGTGCTCAACGACATCGACGAGCTGGCCAAAC
TGCAGCTGATCCCCGCCGATGGCGCGGTGGCGGAAGCCTCGCCGGGCCAGTTTGAAATCAACCTTCACCATACCGATAAC
GTGCTGGACGCCTGCGATGATGCGCTGGCGTTAAAACGTCTCGTGCGTTTGATGGCGGAGAAGCATAAGATGCACGCCAC
TTTTATGGCGAAGCCTTATGAAGAGCATGCCGGCAGTGGGATGCATATCCACATCAGCATGCTGAACAATAAAGGGGAAA
ACGTGCTGGTGGACGGCGATGGGGAAGACTCTGCGCTGCTCAAACGCGCGCTGGCCGGGATGATCGATCTGATGCCGGCT
TCGATGGCGCTGCTGGCGCCGAACGTGAACTCGTATCGCCGCTTCCAGCCGGGGATGTACGTCCCGACGCAGGCTTCGTG
GGGGCATAACAACCGCACCGTCGCGCTGCGTATTCCGTGCGGCGAGCGGCAGAACCACCGCGTGGAGTATCGCGTCGCGG
GGGCGGACGCCAACCCGTATCTGGTGATGGCGGCGATTTTTGCCGGCATTCTGCACGGGCTGGATAACCCCCAGCTGCCT
TTACAGGAAGAGGTGGAAGGTAACGGTCTGGAACAAGAGGGCCTGCCATTCCCGATCCGCCAGAGCGATGCGCTGTGGGA
GTTTATGCAGAACGATCACTTGCGTGAGCGCCTCGGCGAGCGTTTCTGCCATGTTTTCCATGCCTGTAAGCATGATGAAT
TACTGCAGTTTGAGCGTCTTATCACAGAAACCGAAATTGAGTGGATGTTGAAAAACGCCTGA

Upstream 100 bases:

>100_bases
ACATTTGACAAACAATTTATTTGCATACACAGTCGATGGGGTGGACATTATATTTAACGGTCTGAGTGCAGGACCGAAAC
CCAACCAACGGCGGTGAATC

Downstream 100 bases:

>100_bases
TGGTCGCCCCGCGAGGGGCCCATAAGTTTGGTAATCAGTAGCATCATACGGCCTGAGCGGAGCCTGGCCGACGTTTTTGC
TGGGAGCCCAGCCATTTCCC

Product: putative glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
METNIVEVENFVQQTEERRVSAFAKEVKRYLETYPDTQYVDVLLTDLNGCFRGKRIPVAGLSKLEKGCYFPASVFAMDIL
GNVVEEAGLGQEMGEPDRSCIPVPGTLTPSAADPQSIAQVQLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
EFYLLDRKRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAKLQLIPADGAVAEASPGQFEINLHHTDN
VLDACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMLNNKGENVLVDGDGEDSALLKRALAGMIDLMPA
SMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPCGERQNHRVEYRVAGADANPYLVMAAIFAGILHGLDNPQLP
LQEEVEGNGLEQEGLPFPIRQSDALWEFMQNDHLRERLGERFCHVFHACKHDELLQFERLITETEIEWMLKNA

Sequences:

>Translated_473_residues
METNIVEVENFVQQTEERRVSAFAKEVKRYLETYPDTQYVDVLLTDLNGCFRGKRIPVAGLSKLEKGCYFPASVFAMDIL
GNVVEEAGLGQEMGEPDRSCIPVPGTLTPSAADPQSIAQVQLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
EFYLLDRKRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAKLQLIPADGAVAEASPGQFEINLHHTDN
VLDACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMLNNKGENVLVDGDGEDSALLKRALAGMIDLMPA
SMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPCGERQNHRVEYRVAGADANPYLVMAAIFAGILHGLDNPQLP
LQEEVEGNGLEQEGLPFPIRQSDALWEFMQNDHLRERLGERFCHVFHACKHDELLQFERLITETEIEWMLKNA
>Mature_473_residues
METNIVEVENFVQQTEERRVSAFAKEVKRYLETYPDTQYVDVLLTDLNGCFRGKRIPVAGLSKLEKGCYFPASVFAMDIL
GNVVEEAGLGQEMGEPDRSCIPVPGTLTPSAADPQSIAQVQLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
EFYLLDRKRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAKLQLIPADGAVAEASPGQFEINLHHTDN
VLDACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMLNNKGENVLVDGDGEDSALLKRALAGMIDLMPA
SMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPCGERQNHRVEYRVAGADANPYLVMAAIFAGILHGLDNPQLP
LQEEVEGNGLEQEGLPFPIRQSDALWEFMQNDHLRERLGERFCHVFHACKHDELLQFERLITETEIEWMLKNA

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=440, Percent_Identity=25.6818181818182, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI87081870, Length=473, Percent_Identity=91.1205073995772, Blast_Score=898, Evalue=0.0,
Organism=Escherichia coli, GI1790301, Length=398, Percent_Identity=27.6381909547739, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 53044; Mature: 53044

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METNIVEVENFVQQTEERRVSAFAKEVKRYLETYPDTQYVDVLLTDLNGCFRGKRIPVAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHH
LSKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRSCIPVPGTLTPSAADPQSIAQV
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCCHHHHEE
QLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVELEFYLLDRKRDAEGYLQPPCA
EEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCCCCCCCCCC
PGTDDRNTQSQVYSVDNLNHFADVLNDIDELAKLQLIPADGAVAEASPGQFEINLHHTDN
CCCCCCCCHHHEEECCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEEEEEECCCC
VLDACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMLNNKGENVLVDGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEEEECCCCCEEEEECC
GEDSALLKRALAGMIDLMPASMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPC
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEECC
GERQNHRVEYRVAGADANPYLVMAAIFAGILHGLDNPQLPLQEEVEGNGLEQEGLPFPIR
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCC
QSDALWEFMQNDHLRERLGERFCHVFHACKHDELLQFERLITETEIEWMLKNA
CCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
METNIVEVENFVQQTEERRVSAFAKEVKRYLETYPDTQYVDVLLTDLNGCFRGKRIPVAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHH
LSKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRSCIPVPGTLTPSAADPQSIAQV
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCCHHHHEE
QLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVELEFYLLDRKRDAEGYLQPPCA
EEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCCCCCCCCCC
PGTDDRNTQSQVYSVDNLNHFADVLNDIDELAKLQLIPADGAVAEASPGQFEINLHHTDN
CCCCCCCCHHHEEECCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEEEEEECCCC
VLDACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMLNNKGENVLVDGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEEEECCCCCEEEEECC
GEDSALLKRALAGMIDLMPASMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPC
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEECC
GERQNHRVEYRVAGADANPYLVMAAIFAGILHGLDNPQLPLQEEVEGNGLEQEGLPFPIR
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCC
QSDALWEFMQNDHLRERLGERFCHVFHACKHDELLQFERLITETEIEWMLKNA
CCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]