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The map label for this gene is tktB [C]

Identifier: 152969385

GI number: 152969385

Start: 922556

End: 923401

Strand: Direct

Name: tktB [C]

Synonym: KPN_00828

Alternate gene names: 152969385

Gene position: 922556-923401 (Clockwise)

Preceding gene: 152969384

Following gene: 152969386

Centisome position: 17.36

GC content: 60.28

Gene sequence:

>846_bases
ATGAAGATGACCGAATCACAAAAAGTCGCCGCGGCGGCGTGGCGTATTCGCCGTTACGCCCTGCAAATGGGGGAAGTGCA
GGGGCAGGGCTATATCGGCCAGGCGCTGGGCTATGCCGATGTGCTCGCCACCGCCTTCAGCCATGCCATGACCTATCGCC
CGGAAGATCCCGAGTGGGAGGGGCGCGATCGCTTCCTGCTCTCTCATGGCCATTACGCTATCGCTTATTATGCCGCGCTG
CTGGAGGCGGGCATTATCCCGGAAGCCGAACTGGAAACCTACGGCTCCGACGACAGCCGCCTGCCGATGTCCGGCATGGC
GACCTACACCCCGGGGATGGAGATGTCCGGCGGCTCGCTGGGGCAGGGACTGTCCATTGCCGTCGGCATGGCGCTTGGGC
TGCGGCAGAAACAGAGCAAGGCCTGGGTCTACAACTCCATGTCCGATGGGGAGCTGGACGAAGGGTCGACCTGGGAGGCG
GCGATGTCGGCGGCTCACTATGGCCTGAGCAACCTGATTAACCTTGTCGATGTCAACAAACAGCAGGCTGACGGCGATTC
ACGCAAAATCCTCGGCTTTGAGCCGCTGCAGGATAAATGGGCCGCCTTCGGCTGGTACGTACAGCGCGTGGACGGTAACG
ATCTGCCGGCGGTGATGGCCGCCTTTGACAACGCGAAAAGTTATTCCGGCAATCAGCCGCGGGTCATTTTATGCGACACG
CTGATGGGCAAAGGGGTGCCGTTCCTCGAAACCCGCGATAAAAACCATTTTATTCGCGTCGATGCCGACGAATGGCAAAA
AGCGATCGCGGTGCTGGACGCTAACAAACCGGAAGGAGTGCTGTAA

Upstream 100 bases:

>100_bases
CGCGCCGCGCTGTTCCTCGGCAGCGATCTTTCCTCCTACTCCACCGGCATCACCCTGGACGTTAACGGCGGCATGTTAAT
TCACTAAGCGAGGCAAACAG

Downstream 100 bases:

>100_bases
TGAGCCAGAGTCAACCCCGTTTAAAAACGTCGGCGATGATCGCCTCGATTGCTGATGAAGGCCAGGCGACCGTCCCTGCG
CCCTTCGGCGTCGCGCTGTC

Product: putative transketolase

Products: D-ribose 5-phosphate; D-xylulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MKMTESQKVAAAAWRIRRYALQMGEVQGQGYIGQALGYADVLATAFSHAMTYRPEDPEWEGRDRFLLSHGHYAIAYYAAL
LEAGIIPEAELETYGSDDSRLPMSGMATYTPGMEMSGGSLGQGLSIAVGMALGLRQKQSKAWVYNSMSDGELDEGSTWEA
AMSAAHYGLSNLINLVDVNKQQADGDSRKILGFEPLQDKWAAFGWYVQRVDGNDLPAVMAAFDNAKSYSGNQPRVILCDT
LMGKGVPFLETRDKNHFIRVDADEWQKAIAVLDANKPEGVL

Sequences:

>Translated_281_residues
MKMTESQKVAAAAWRIRRYALQMGEVQGQGYIGQALGYADVLATAFSHAMTYRPEDPEWEGRDRFLLSHGHYAIAYYAAL
LEAGIIPEAELETYGSDDSRLPMSGMATYTPGMEMSGGSLGQGLSIAVGMALGLRQKQSKAWVYNSMSDGELDEGSTWEA
AMSAAHYGLSNLINLVDVNKQQADGDSRKILGFEPLQDKWAAFGWYVQRVDGNDLPAVMAAFDNAKSYSGNQPRVILCDT
LMGKGVPFLETRDKNHFIRVDADEWQKAIAVLDANKPEGVL
>Mature_281_residues
MKMTESQKVAAAAWRIRRYALQMGEVQGQGYIGQALGYADVLATAFSHAMTYRPEDPEWEGRDRFLLSHGHYAIAYYAAL
LEAGIIPEAELETYGSDDSRLPMSGMATYTPGMEMSGGSLGQGLSIAVGMALGLRQKQSKAWVYNSMSDGELDEGSTWEA
AMSAAHYGLSNLINLVDVNKQQADGDSRKILGFEPLQDKWAAFGWYVQRVDGNDLPAVMAAFDNAKSYSGNQPRVILCDT
LMGKGVPFLETRDKNHFIRVDADEWQKAIAVLDANKPEGVL

Specific function: Unknown

COG id: COG3959

COG function: function code G; Transketolase, N-terminal subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transketolase family [H]

Homologues:

Organism=Homo sapiens, GI133778974, Length=264, Percent_Identity=35.9848484848485, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI205277463, Length=254, Percent_Identity=34.6456692913386, Blast_Score=131, Evalue=6e-31,
Organism=Homo sapiens, GI4507521, Length=254, Percent_Identity=34.6456692913386, Blast_Score=131, Evalue=6e-31,
Organism=Homo sapiens, GI225637459, Length=255, Percent_Identity=28.2352941176471, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI225637461, Length=228, Percent_Identity=29.8245614035088, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI225637463, Length=226, Percent_Identity=30.0884955752212, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788808, Length=251, Percent_Identity=32.6693227091633, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI48994911, Length=242, Percent_Identity=30.5785123966942, Blast_Score=108, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17539652, Length=249, Percent_Identity=33.3333333333333, Blast_Score=124, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6325331, Length=258, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319593, Length=257, Percent_Identity=29.5719844357977, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI45551847, Length=253, Percent_Identity=31.2252964426877, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI45550715, Length=253, Percent_Identity=31.2252964426877, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24666278, Length=254, Percent_Identity=29.9212598425197, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24645119, Length=205, Percent_Identity=35.609756097561, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005474 [H]

Pfam domain/function: PF00456 Transketolase_N [H]

EC number: 2.2.1.1

Molecular weight: Translated: 30833; Mature: 30833

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMTESQKVAAAAWRIRRYALQMGEVQGQGYIGQALGYADVLATAFSHAMTYRPEDPEWE
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GRDRFLLSHGHYAIAYYAALLEAGIIPEAELETYGSDDSRLPMSGMATYTPGMEMSGGSL
CCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCC
GQGLSIAVGMALGLRQKQSKAWVYNSMSDGELDEGSTWEAAMSAAHYGLSNLINLVDVNK
CCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCC
QQADGDSRKILGFEPLQDKWAAFGWYVQRVDGNDLPAVMAAFDNAKSYSGNQPRVILCDT
HHCCCCCCEEECCCCCHHHHHHHHEEEEEECCCCCHHHHHHHHCCCCCCCCCCEEEEEEC
LMGKGVPFLETRDKNHFIRVDADEWQKAIAVLDANKPEGVL
HHCCCCCCEEECCCCCEEEECHHHHHHHHHEEECCCCCCCC
>Mature Secondary Structure
MKMTESQKVAAAAWRIRRYALQMGEVQGQGYIGQALGYADVLATAFSHAMTYRPEDPEWE
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GRDRFLLSHGHYAIAYYAALLEAGIIPEAELETYGSDDSRLPMSGMATYTPGMEMSGGSL
CCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCC
GQGLSIAVGMALGLRQKQSKAWVYNSMSDGELDEGSTWEAAMSAAHYGLSNLINLVDVNK
CCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCC
QQADGDSRKILGFEPLQDKWAAFGWYVQRVDGNDLPAVMAAFDNAKSYSGNQPRVILCDT
HHCCCCCCEEECCCCCHHHHHHHHEEEEEECCCCCHHHHHHHHCCCCCCCCCCEEEEEEC
LMGKGVPFLETRDKNHFIRVDADEWQKAIAVLDANKPEGVL
HHCCCCCCEEECCCCCEEEECHHHHHHHHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Thiamine diphosphate, mono-or triphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sedoheptulose 7-phosphate; D-glyceraldehyde 3-phosphate

Specific reaction: sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate

General reaction: Keto group transfer [C]

Inhibitor: EDTA; Oxythiamine diphosphate; Phosphate; Rabbit Antibodies; Sulfate [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]