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The map label for this gene is mepA [H]

Identifier: 150398556

GI number: 150398556

Start: 3568690

End: 3569757

Strand: Direct

Name: mepA [H]

Synonym: Smed_3367

Alternate gene names: 150398556

Gene position: 3568690-3569757 (Clockwise)

Preceding gene: 150398550

Following gene: 150398557

Centisome position: 94.36

GC content: 65.17

Gene sequence:

>1068_bases
ATGAGATTTGATGCGCGTGCTGCGCTTAGGCGCTGCGGTTCGGCCGTCCTGGCTCTGATCATGGGGGCAAGCCTCGTCTC
CGCGGAGGCGGCTGCGGACGACGCTCCGCCGGCCAAGGAACTCTTCGGCGCCAAAGGCCTGCCGATGCAGGCGGCGCCGG
CCGCGTTCGGATTCTACTCCAAGGGCTGCCTCGCCGGTGGCGTGGCCATCCCGACCGACGGACCGACATGGCAGGCGATG
CGCCTGTCGCGAAACCGCCGCTGGGGACATCCGGCGATGATCGCGCTGATCGAGCGCTTCTCCCATGATGCCGTCGACAA
GATCGGCTGGCCTGGCCTCTTGCTCGGCGATATCTCGCAGCCGCGCGGCGGCCCGATGCTTTCGGGCCACGCATCGCACC
AGATCGGGCTCGATGCCGACATTTGGCTGACTCCGATGCCGCGAAAGACGCTGAGCTACCAGGAGCGCGAGACGATTTCC
GCGACGTCCATGCTCGATAAGAGCAAGTTCCTGACGGTCGATCCCTCCATCTGGACGCCATCGCACGCACGGCTGATCAT
GATGGCAGCCAGCTATCCGCAGGTCGAGCGGGTCTTCGTGAACCCCGCTATCAAAAAGAAGCTCTGCGACACCTGGAAAG
GCGATCGTTCGGCGCTCGGCAAGGTGCGGCCGATCTATGGCCATGATTATCACTTCCACATCAGGATCAAATGCCCTCCG
GGCTCCAAGGGCTGCAAGGACCAGGCCGCGGTTCCGGCGGGTGACGGCTGCGGCAAGTCGCTGGCGTGGTGGTTCACCGA
CGAGCCCTGGGCAAAGCCGAAGCAAAAGCCCGCTGCGAAGCCGCCGCGGCCGAAATTCGCGAAACTCTCCGATCTTCCGA
AGGCCTGCGCCCTTGTGCTGAACGGCCCCGCTCCCTCTTCCGAACAGGAAGCGACCTACGGTACTGCCTATCGCGCTCCC
GCCGCCATTCCTGCAGCGGCAACCAGCATCGAAGCGGTCATCGAAGCGGCAGGCGAGGCGCCGCTCGCGGATATTCCCGT
GCCTTTGCCGCGGCCGGGCTTGGAATAG

Upstream 100 bases:

>100_bases
TCAGGATAGGAGAGGACGGCGGGCTGCGGCGCCGCGCGCCGTGTCAGAGGCGCAAAAATCGCCGTAGCTCCAAAGTTGCC
GCATGCTCGTGGGGAATGGA

Downstream 100 bases:

>100_bases
CCGCGGGCGCGCGAACAATAAGGTGCATGTTTTCAGGCCGATCGATCCCAAACATGCACGATACCGATTCCAGTAGGATA
GAGCGGGATTTCGGCGGAAA

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MRFDARAALRRCGSAVLALIMGASLVSAEAAADDAPPAKELFGAKGLPMQAAPAAFGFYSKGCLAGGVAIPTDGPTWQAM
RLSRNRRWGHPAMIALIERFSHDAVDKIGWPGLLLGDISQPRGGPMLSGHASHQIGLDADIWLTPMPRKTLSYQERETIS
ATSMLDKSKFLTVDPSIWTPSHARLIMMAASYPQVERVFVNPAIKKKLCDTWKGDRSALGKVRPIYGHDYHFHIRIKCPP
GSKGCKDQAAVPAGDGCGKSLAWWFTDEPWAKPKQKPAAKPPRPKFAKLSDLPKACALVLNGPAPSSEQEATYGTAYRAP
AAIPAAATSIEAVIEAAGEAPLADIPVPLPRPGLE

Sequences:

>Translated_355_residues
MRFDARAALRRCGSAVLALIMGASLVSAEAAADDAPPAKELFGAKGLPMQAAPAAFGFYSKGCLAGGVAIPTDGPTWQAM
RLSRNRRWGHPAMIALIERFSHDAVDKIGWPGLLLGDISQPRGGPMLSGHASHQIGLDADIWLTPMPRKTLSYQERETIS
ATSMLDKSKFLTVDPSIWTPSHARLIMMAASYPQVERVFVNPAIKKKLCDTWKGDRSALGKVRPIYGHDYHFHIRIKCPP
GSKGCKDQAAVPAGDGCGKSLAWWFTDEPWAKPKQKPAAKPPRPKFAKLSDLPKACALVLNGPAPSSEQEATYGTAYRAP
AAIPAAATSIEAVIEAAGEAPLADIPVPLPRPGLE
>Mature_355_residues
MRFDARAALRRCGSAVLALIMGASLVSAEAAADDAPPAKELFGAKGLPMQAAPAAFGFYSKGCLAGGVAIPTDGPTWQAM
RLSRNRRWGHPAMIALIERFSHDAVDKIGWPGLLLGDISQPRGGPMLSGHASHQIGLDADIWLTPMPRKTLSYQERETIS
ATSMLDKSKFLTVDPSIWTPSHARLIMMAASYPQVERVFVNPAIKKKLCDTWKGDRSALGKVRPIYGHDYHFHIRIKCPP
GSKGCKDQAAVPAGDGCGKSLAWWFTDEPWAKPKQKPAAKPPRPKFAKLSDLPKACALVLNGPAPSSEQEATYGTAYRAP
AAIPAAATSIEAVIEAAGEAPLADIPVPLPRPGLE

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=254, Percent_Identity=37.007874015748, Blast_Score=164, Evalue=8e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 37969; Mature: 37969

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDARAALRRCGSAVLALIMGASLVSAEAAADDAPPAKELFGAKGLPMQAAPAAFGFYS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCHHHHHHH
KGCLAGGVAIPTDGPTWQAMRLSRNRRWGHPAMIALIERFSHDAVDKIGWPGLLLGDISQ
CCHHCCCEECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
PRGGPMLSGHASHQIGLDADIWLTPMPRKTLSYQERETISATSMLDKSKFLTVDPSIWTP
CCCCCEECCCCCCCCCCCCCEEECCCCHHHHCHHHHHHHHHHHHHCCCCEEEECCCCCCC
SHARLIMMAASYPQVERVFVNPAIKKKLCDTWKGDRSALGKVRPIYGHDYHFHIRIKCPP
CCCEEEEEECCCCCCEEEEECHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEEEEEECCC
GSKGCKDQAAVPAGDGCGKSLAWWFTDEPWAKPKQKPAAKPPRPKFAKLSDLPKACALVL
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEE
NGPAPSSEQEATYGTAYRAPAAIPAAATSIEAVIEAAGEAPLADIPVPLPRPGLE
CCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRFDARAALRRCGSAVLALIMGASLVSAEAAADDAPPAKELFGAKGLPMQAAPAAFGFYS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCHHHHHHH
KGCLAGGVAIPTDGPTWQAMRLSRNRRWGHPAMIALIERFSHDAVDKIGWPGLLLGDISQ
CCHHCCCEECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
PRGGPMLSGHASHQIGLDADIWLTPMPRKTLSYQERETISATSMLDKSKFLTVDPSIWTP
CCCCCEECCCCCCCCCCCCCEEECCCCHHHHCHHHHHHHHHHHHHCCCCEEEECCCCCCC
SHARLIMMAASYPQVERVFVNPAIKKKLCDTWKGDRSALGKVRPIYGHDYHFHIRIKCPP
CCCEEEEEECCCCCCEEEEECHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEEEEEECCC
GSKGCKDQAAVPAGDGCGKSLAWWFTDEPWAKPKQKPAAKPPRPKFAKLSDLPKACALVL
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEE
NGPAPSSEQEATYGTAYRAPAAIPAAATSIEAVIEAAGEAPLADIPVPLPRPGLE
CCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]