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The map label for this gene is trpA [H]

Identifier: 150398430

GI number: 150398430

Start: 3413036

End: 3413875

Strand: Direct

Name: trpA [H]

Synonym: Smed_3238

Alternate gene names: 150398430

Gene position: 3413036-3413875 (Clockwise)

Preceding gene: 150398429

Following gene: 150398431

Centisome position: 90.25

GC content: 64.29

Gene sequence:

>840_bases
ATGACCGCACGCATGGAGCAAAGGTTCGCCGATGTGGCGGCTGAAGGGCGCCCGGTTCTCGTGACCTATTTCATGGGCGG
CGACCCGGATTTCGACACGTCGCTCGCGATCATGAAGGCGCTGCAGCAGGCCGGCGCCGATATCATCGAACTCGGCGTCC
CCTTTTCCGACCCGATGGCCGATGGACCGGCGATCCAGCTCGCCGGTCAGCGTGCGCTCAAGGCCGGGCAGACGCTTGCC
AAGACGCTGGAGCTTGCCCGGCGTTTCCGGGCCGACGATCAGCGTACCCCCATCGTGCTGATGGGCTACTACAATCCCAT
CTACATCTACGGCGTCGAGCGGTTTTTGACGGACGCGCTCGAGGCCGGCGTCGACGGCATGATCGTCGTCGACCTGCCGC
CGGAAATGGACGACGAACTCTGCATTCCGGCGCTCGCGAAGGGCATCAGCTTCATCCGCCTGGCTACACCGACGACGGAT
GACCGGCGTCTGCCCAAAGTCCTCGAAAATACGTCCGGTTTCGTGTATTACGTGTCGATGACCGGTATCACCGGCTCGGC
GCTGCCGGATACGTCGCTGATTGCCGGTGCCGTGGCGCGCATCAAGGCACATACCTCCTTGCCGGTCTGCGTCGGTTTCG
GGGTGAAGACGGCGGATCATGCACGCGCCATTGGTGCAACCGCCGATGGCGTCGTCGTCGGCACGGCCATCGTCAATCAG
ATCGCGTCGAGTTTGACGGAAGAGGGCCGCGCAACGGAAGCGACGGTTCCGGGTGTCGAAGCGCTCGTCAGAGGGCTTGC
GGCCGGCGTACGGGCGGCACGACTCGCCGCAGCCGAATAA

Upstream 100 bases:

>100_bases
AAATGGGCAAGGACGAGATCATCCTGATGAATCTCTCCGGTCGCGGCGACAAGGACATCTTCACCGTCGGCAAAATTCTC
GGTATGGGGCAATAACGAAC

Downstream 100 bases:

>100_bases
TTGCCCATATTGACGCGCAAAAGCCGAAGGAAAATCTTCAGGAGAATGCTTAATTGAACTGGATCACAAACTACGTTCGG
CCGAAGATCAATTCGATGCT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTARMEQRFADVAAEGRPVLVTYFMGGDPDFDTSLAIMKALQQAGADIIELGVPFSDPMADGPAIQLAGQRALKAGQTLA
KTLELARRFRADDQRTPIVLMGYYNPIYIYGVERFLTDALEAGVDGMIVVDLPPEMDDELCIPALAKGISFIRLATPTTD
DRRLPKVLENTSGFVYYVSMTGITGSALPDTSLIAGAVARIKAHTSLPVCVGFGVKTADHARAIGATADGVVVGTAIVNQ
IASSLTEEGRATEATVPGVEALVRGLAAGVRAARLAAAE

Sequences:

>Translated_279_residues
MTARMEQRFADVAAEGRPVLVTYFMGGDPDFDTSLAIMKALQQAGADIIELGVPFSDPMADGPAIQLAGQRALKAGQTLA
KTLELARRFRADDQRTPIVLMGYYNPIYIYGVERFLTDALEAGVDGMIVVDLPPEMDDELCIPALAKGISFIRLATPTTD
DRRLPKVLENTSGFVYYVSMTGITGSALPDTSLIAGAVARIKAHTSLPVCVGFGVKTADHARAIGATADGVVVGTAIVNQ
IASSLTEEGRATEATVPGVEALVRGLAAGVRAARLAAAE
>Mature_278_residues
TARMEQRFADVAAEGRPVLVTYFMGGDPDFDTSLAIMKALQQAGADIIELGVPFSDPMADGPAIQLAGQRALKAGQTLAK
TLELARRFRADDQRTPIVLMGYYNPIYIYGVERFLTDALEAGVDGMIVVDLPPEMDDELCIPALAKGISFIRLATPTTDD
RRLPKVLENTSGFVYYVSMTGITGSALPDTSLIAGAVARIKAHTSLPVCVGFGVKTADHARAIGATADGVVVGTAIVNQI
ASSLTEEGRATEATVPGVEALVRGLAAGVRAARLAAAE

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=274, Percent_Identity=32.4817518248175, Blast_Score=133, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6321412, Length=266, Percent_Identity=41.3533834586466, Blast_Score=202, Evalue=5e-53,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 29339; Mature: 29208

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARMEQRFADVAAEGRPVLVTYFMGGDPDFDTSLAIMKALQQAGADIIELGVPFSDPMA
CCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCC
DGPAIQLAGQRALKAGQTLAKTLELARRFRADDQRTPIVLMGYYNPIYIYGVERFLTDAL
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEHHHHHHHHH
EAGVDGMIVVDLPPEMDDELCIPALAKGISFIRLATPTTDDRRLPKVLENTSGFVYYVSM
HCCCCCEEEEECCCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEEEE
TGITGSALPDTSLIAGAVARIKAHTSLPVCVGFGVKTADHARAIGATADGVVVGTAIVNQ
ECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHCCCCCCCEEHHHHHHHH
IASSLTEEGRATEATVPGVEALVRGLAAGVRAARLAAAE
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TARMEQRFADVAAEGRPVLVTYFMGGDPDFDTSLAIMKALQQAGADIIELGVPFSDPMA
CCHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCC
DGPAIQLAGQRALKAGQTLAKTLELARRFRADDQRTPIVLMGYYNPIYIYGVERFLTDAL
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEHHHHHHHHH
EAGVDGMIVVDLPPEMDDELCIPALAKGISFIRLATPTTDDRRLPKVLENTSGFVYYVSM
HCCCCCEEEEECCCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEEEE
TGITGSALPDTSLIAGAVARIKAHTSLPVCVGFGVKTADHARAIGATADGVVVGTAIVNQ
ECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHCCCCCCCEEHHHHHHHH
IASSLTEEGRATEATVPGVEALVRGLAAGVRAARLAAAE
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]