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The map label for this gene is dapF [H]

Identifier: 150398287

GI number: 150398287

Start: 3239739

End: 3240617

Strand: Reverse

Name: dapF [H]

Synonym: Smed_3093

Alternate gene names: 150398287

Gene position: 3240617-3239739 (Counterclockwise)

Preceding gene: 150398288

Following gene: 150398286

Centisome position: 85.69

GC content: 64.62

Gene sequence:

>879_bases
ATGAACGGGCTTGGAAACAAGATCCTGGTGGTCGACATGCGTGGCCGCAAGGACCGCGTGACGCGTCAGGCTGCAATAGT
GCTCAACGCCGACCCGGCAACCGAGTTCGACCAGATCATGGCGATCCATGATCCGAAGTCCGCCGGCACCGACGCGTGGA
TCGACATCGTCAATTCCGACGGGTCGATGGCCCAGGCCTGCGGCAACGGCACCCGCTGCGTGGTCCAGGCGCTTGCGGCC
GAGACCGGCAAGAAGGCCTTCCTCTTCCACACGGTTGCGGGCCTCCTTGAAGCGAAGGAGCATGACGACGGCTCTATTTC
GGTCGATATGGGCAAGCCGCGTTTCCGCTGGGACCAGATACCGCTCGCCGAGGAGTTCCATGACACGCGCCGGATCGAGC
TGCAGATCGGACCCATCGAGGCTCCCGTGCTGCACTCTCCTTCCGTTGTATCCATGGGCAATCCTCACGCGATCTTCTGG
GTCGAAAACGACGTGTCCGGCTACGATCTCGATCGTTTCGGACCGCTCCTCGAGAACCACCCGATCTTCCCTGAACGGGC
CAATATATCGGTCGCCCGCATCCGCTCCCGCCAGGAGATGGACCTGCGGACCTGGGAGCGCGGCGCCGGCCTGACGCTTG
CCTGCGGCTCGGCCGCCTGTGCCGCTGCCGTCAGCGGTGCGAGAACAGGCCGCACGGAGCGCGCGGTGACCGTAAATGTG
CCCGGAGGACCACTTCGGATCGAATGGCGCGAAAGCGACGACCACGTCATCATGACCGGCCCGGCCGAATGGGAATGGTC
CGGCACGCTCGACCCTGTCACCGGCATTTTCGAGCGGAACGAGCCGGAGAGCGGCGACATCGGAGCCCGAGCGCCTTGA

Upstream 100 bases:

>100_bases
GGGCTTTTCCGCCATATACAGCCCGAATGAAGGGCGCCTCCGGAACGGAAGGCCAGGTTTGGATAATCGCGACATGGCCG
ATCAGGTGCAATTTGCCAGG

Downstream 100 bases:

>100_bases
GCGGCGTCGAGGTCATAACCTTCGGCTGCCGTCTGAACACCTACGAATCGGAAGTGATGCGGGCAGAGGCCGAAAAGGCG
GGGCTGAACAATGCCGTGCT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MNGLGNKILVVDMRGRKDRVTRQAAIVLNADPATEFDQIMAIHDPKSAGTDAWIDIVNSDGSMAQACGNGTRCVVQALAA
ETGKKAFLFHTVAGLLEAKEHDDGSISVDMGKPRFRWDQIPLAEEFHDTRRIELQIGPIEAPVLHSPSVVSMGNPHAIFW
VENDVSGYDLDRFGPLLENHPIFPERANISVARIRSRQEMDLRTWERGAGLTLACGSAACAAAVSGARTGRTERAVTVNV
PGGPLRIEWRESDDHVIMTGPAEWEWSGTLDPVTGIFERNEPESGDIGARAP

Sequences:

>Translated_292_residues
MNGLGNKILVVDMRGRKDRVTRQAAIVLNADPATEFDQIMAIHDPKSAGTDAWIDIVNSDGSMAQACGNGTRCVVQALAA
ETGKKAFLFHTVAGLLEAKEHDDGSISVDMGKPRFRWDQIPLAEEFHDTRRIELQIGPIEAPVLHSPSVVSMGNPHAIFW
VENDVSGYDLDRFGPLLENHPIFPERANISVARIRSRQEMDLRTWERGAGLTLACGSAACAAAVSGARTGRTERAVTVNV
PGGPLRIEWRESDDHVIMTGPAEWEWSGTLDPVTGIFERNEPESGDIGARAP
>Mature_292_residues
MNGLGNKILVVDMRGRKDRVTRQAAIVLNADPATEFDQIMAIHDPKSAGTDAWIDIVNSDGSMAQACGNGTRCVVQALAA
ETGKKAFLFHTVAGLLEAKEHDDGSISVDMGKPRFRWDQIPLAEEFHDTRRIELQIGPIEAPVLHSPSVVSMGNPHAIFW
VENDVSGYDLDRFGPLLENHPIFPERANISVARIRSRQEMDLRTWERGAGLTLACGSAACAAAVSGARTGRTERAVTVNV
PGGPLRIEWRESDDHVIMTGPAEWEWSGTLDPVTGIFERNEPESGDIGARAP

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=277, Percent_Identity=35.7400722021661, Blast_Score=152, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 31783; Mature: 31783

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGLGNKILVVDMRGRKDRVTRQAAIVLNADPATEFDQIMAIHDPKSAGTDAWIDIVNSD
CCCCCCEEEEEEECCCCHHCEEEEEEEEECCCCCCCHHEEEEECCCCCCCCEEEEEECCC
GSMAQACGNGTRCVVQALAAETGKKAFLFHTVAGLLEAKEHDDGSISVDMGKPRFRWDQI
CCHHHHCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCEEEEECCCCCCEECCC
PLAEEFHDTRRIELQIGPIEAPVLHSPSVVSMGNPHAIFWVENDVSGYDLDRFGPLLENH
CCHHHHCCCEEEEEEECCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCHHHHCCHHHCC
PIFPERANISVARIRSRQEMDLRTWERGAGLTLACGSAACAAAVSGARTGRTERAVTVNV
CCCCCCCCEEHHHHCCCCCCCHHHHHCCCCEEEEECCHHHHHHHHCCCCCCCCCEEEEEC
PGGPLRIEWRESDDHVIMTGPAEWEWSGTLDPVTGIFERNEPESGDIGARAP
CCCEEEEEEECCCCEEEEECCCCEECCCCCCHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNGLGNKILVVDMRGRKDRVTRQAAIVLNADPATEFDQIMAIHDPKSAGTDAWIDIVNSD
CCCCCCEEEEEEECCCCHHCEEEEEEEEECCCCCCCHHEEEEECCCCCCCCEEEEEECCC
GSMAQACGNGTRCVVQALAAETGKKAFLFHTVAGLLEAKEHDDGSISVDMGKPRFRWDQI
CCHHHHCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCEEEEECCCCCCEECCC
PLAEEFHDTRRIELQIGPIEAPVLHSPSVVSMGNPHAIFWVENDVSGYDLDRFGPLLENH
CCHHHHCCCEEEEEEECCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCHHHHCCHHHCC
PIFPERANISVARIRSRQEMDLRTWERGAGLTLACGSAACAAAVSGARTGRTERAVTVNV
CCCCCCCCEEHHHHCCCCCCCHHHHHCCCCEEEEECCHHHHHHHHCCCCCCCCCEEEEEC
PGGPLRIEWRESDDHVIMTGPAEWEWSGTLDPVTGIFERNEPESGDIGARAP
CCCEEEEEEECCCCEEEEECCCCEECCCCCCHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]