Click here to switch to the map view.

The map label for this gene is nuoM [H]

Identifier: 150396125

GI number: 150396125

Start: 973205

End: 974716

Strand: Direct

Name: nuoM [H]

Synonym: Smed_0902

Alternate gene names: 150396125

Gene position: 973205-974716 (Clockwise)

Preceding gene: 150396124

Following gene: 150396126

Centisome position: 25.73

GC content: 59.99

Gene sequence:

>1512_bases
ATGACCGATTGGCCCGTACTTTCCGCGGTCACCTTCATGCCGCTCGTCGGCGTCCTGCTTCTCTTGCTGACAAGAGAAGA
CAGCGCCTACGGCCGCCGCAACATCCTGAACGTCTCGCTTCTGACGACGATCTTCACGTTTCTCGTATCGCTCTACGTCT
GGTACCAGTTCGACCCGTCGAATCCCGGTTTCCAGATGATCGAAAAGCGGGAGTGGCTCGGGGCCGGCATTTCCTACCAC
CTCGGCGTGGACGGCATATCGATCCTCTTCGTGCCGCTGACCGCGTTTCTGATGCCATTCTGTGTTCTCGCAAGCTGGGT
GACCATCGAGAAGCGCCTGAAGGAATACATGATCGCGTTTCTTATTCTGGAAACGCTGATGCTGGGCGTCTTCGTGTCGC
TCGACATCATCCTCTTCTACGTCTTCTTCGAAGCCGGCCTCATTCCGATGTTCATCATCATCGGCGTATGGGGTGGCAAG
GAGCGCGTCTACGCGAGCTATAAGTTCTTCCTCTATACGCTGCTCGGCTCCGTTCTGATGCTGCTCGCCATGATGGCGAT
GTACTGGCAGGCGGGCACGACCGATATCACGCAATTGCTCGCCTATCAGTTCCCGCGCCAGATGCAGACCTGGCTCTGGC
TTGCCTTCTTCGCCTCCTTCGCGGTGAAGATGCCGATGTGGCCGGTGCATACCTGGCTTCCCGATGCGCACGTGCAGGCG
CCGACGGCAGGCTCGGTCATCCTGGCGGGTATCCTCCTGAAGCTCGGCGGCTACGGCTTCCTGCGCTTCTCGCTGCCGAT
GTTCCCGCTGGCGTCCGACTTCTTCGCGCCCTTCGTCTTCACGCTGTCGGTCATCGCGATCATCTACACCTCTCTGGTGG
CGATGATGCAGACGGACATCAAGAAGTTGATCGCCTATTCGTCCGTAGCCCATATGGGCTATGTGACGATGGGAACCTTC
GCGGCGAACGTGCAGGGGGTTCAGGGCGCTATCTTCCAGATGCTCTCGCATGGCATCGTCTCGGGCGCGCTCTTCCTTTG
CGTCGGCGTCGTCTATGACCGGCTGCATACGCGCGAGATCGCGGCCTATGGCGGCCTCGTCAACAACATGCCGAAATACG
CCGTCGCCTTCATGGTCTTCACCATGGCCAATGTCGGCCTGCCCGGGACGTCGGGCTTCGTCGGCGAAGTGCTGACGCTT
GTGGGTGCTTTCCGCGCCAATACCTGGGTCGCGCTCTTCGCGACGAGCGGCGTCATTCTGTCGGCGGCCTACGCGCTGTG
GCTCTACCGCCGCGTCATTTTCGGCGCGCTGGAAAAGGAAAGCCTGAAGGCGTTGCTCGATCTCAGCCCGCGTGAGAAGC
TCATTCTCTATCCGCTTGTTATCCTGACCATCTTCTTCGGTGTCTATCCGGCACCGGTCTTCGACGCGACCGCGGCCTCC
GTGGACCTGCTCGTCAACAACTACGCCGCCGCCCTGCAGGCAGCGCAAGACGTTGCACTTTCGGTGCAATGA

Upstream 100 bases:

>100_bases
TTGTCCGCCTGCAGACCGGATACCTTTACCACTACGCGTTCGCCATGCTGATCGGCATCGCGGCGCTCGTTACATGGATG
ATGCTCGGGAGTTCCTTCTG

Downstream 100 bases:

>100_bases
TCAAGGGACGTGATGTGACATGACTGCTGAAACCCTTATTGCCAGCCTGCAACTTTCGATGCCCGAGCTGATCCTTGCGG
TCGGCGCGATGGCGCTCCTG

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MTDWPVLSAVTFMPLVGVLLLLLTREDSAYGRRNILNVSLLTTIFTFLVSLYVWYQFDPSNPGFQMIEKREWLGAGISYH
LGVDGISILFVPLTAFLMPFCVLASWVTIEKRLKEYMIAFLILETLMLGVFVSLDIILFYVFFEAGLIPMFIIIGVWGGK
ERVYASYKFFLYTLLGSVLMLLAMMAMYWQAGTTDITQLLAYQFPRQMQTWLWLAFFASFAVKMPMWPVHTWLPDAHVQA
PTAGSVILAGILLKLGGYGFLRFSLPMFPLASDFFAPFVFTLSVIAIIYTSLVAMMQTDIKKLIAYSSVAHMGYVTMGTF
AANVQGVQGAIFQMLSHGIVSGALFLCVGVVYDRLHTREIAAYGGLVNNMPKYAVAFMVFTMANVGLPGTSGFVGEVLTL
VGAFRANTWVALFATSGVILSAAYALWLYRRVIFGALEKESLKALLDLSPREKLILYPLVILTIFFGVYPAPVFDATAAS
VDLLVNNYAAALQAAQDVALSVQ

Sequences:

>Translated_503_residues
MTDWPVLSAVTFMPLVGVLLLLLTREDSAYGRRNILNVSLLTTIFTFLVSLYVWYQFDPSNPGFQMIEKREWLGAGISYH
LGVDGISILFVPLTAFLMPFCVLASWVTIEKRLKEYMIAFLILETLMLGVFVSLDIILFYVFFEAGLIPMFIIIGVWGGK
ERVYASYKFFLYTLLGSVLMLLAMMAMYWQAGTTDITQLLAYQFPRQMQTWLWLAFFASFAVKMPMWPVHTWLPDAHVQA
PTAGSVILAGILLKLGGYGFLRFSLPMFPLASDFFAPFVFTLSVIAIIYTSLVAMMQTDIKKLIAYSSVAHMGYVTMGTF
AANVQGVQGAIFQMLSHGIVSGALFLCVGVVYDRLHTREIAAYGGLVNNMPKYAVAFMVFTMANVGLPGTSGFVGEVLTL
VGAFRANTWVALFATSGVILSAAYALWLYRRVIFGALEKESLKALLDLSPREKLILYPLVILTIFFGVYPAPVFDATAAS
VDLLVNNYAAALQAAQDVALSVQ
>Mature_502_residues
TDWPVLSAVTFMPLVGVLLLLLTREDSAYGRRNILNVSLLTTIFTFLVSLYVWYQFDPSNPGFQMIEKREWLGAGISYHL
GVDGISILFVPLTAFLMPFCVLASWVTIEKRLKEYMIAFLILETLMLGVFVSLDIILFYVFFEAGLIPMFIIIGVWGGKE
RVYASYKFFLYTLLGSVLMLLAMMAMYWQAGTTDITQLLAYQFPRQMQTWLWLAFFASFAVKMPMWPVHTWLPDAHVQAP
TAGSVILAGILLKLGGYGFLRFSLPMFPLASDFFAPFVFTLSVIAIIYTSLVAMMQTDIKKLIAYSSVAHMGYVTMGTFA
ANVQGVQGAIFQMLSHGIVSGALFLCVGVVYDRLHTREIAAYGGLVNNMPKYAVAFMVFTMANVGLPGTSGFVGEVLTLV
GAFRANTWVALFATSGVILSAAYALWLYRRVIFGALEKESLKALLDLSPREKLILYPLVILTIFFGVYPAPVFDATAASV
DLLVNNYAAALQAAQDVALSVQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=367, Percent_Identity=34.8773841961853, Blast_Score=199, Evalue=7e-51,
Organism=Homo sapiens, GI251831117, Length=401, Percent_Identity=22.1945137157107, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1788613, Length=488, Percent_Identity=38.5245901639344, Blast_Score=304, Evalue=8e-84,
Organism=Escherichia coli, GI1788827, Length=308, Percent_Identity=27.5974025974026, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1788831, Length=303, Percent_Identity=27.3927392739274, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788614, Length=483, Percent_Identity=24.4306418219462, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI145693160, Length=314, Percent_Identity=25.4777070063694, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI2367154, Length=240, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788829, Length=482, Percent_Identity=22.8215767634855, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55742; Mature: 55611

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TDWPVLSAVTFMPLVGVLLLLLTREDSAYGRRNILNVSLLTTIFTFLVSLYVWYQFDPS
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCC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HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]