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The map label for this gene is nuoN1 [H]

Identifier: 150396126

GI number: 150396126

Start: 974736

End: 976178

Strand: Direct

Name: nuoN1 [H]

Synonym: Smed_0903

Alternate gene names: 150396126

Gene position: 974736-976178 (Clockwise)

Preceding gene: 150396125

Following gene: 150396127

Centisome position: 25.77

GC content: 61.19

Gene sequence:

>1443_bases
ATGACTGCTGAAACCCTTATTGCCAGCCTGCAACTTTCGATGCCCGAGCTGATCCTTGCGGTCGGCGCGATGGCGCTCCT
GATGATCGGCGTCTTCTCGAGCGAAAGAGCCACCCCGACCGTGACCGGGCTTGCGGTCGCGGTGCTCATCATCGCCGGGC
TGTGGCTCGTGCTGAAGACGGGCGAGGGGGCGGCTTATGGCGGCGCCTTCCTGTCCGATCCCTTCGCCAAATTCATGAAA
GTGCTCGCTCTCATCGGTTCGATCACAGTGATGGTGATGACCGTCGGTCACGCGCGTTCGGCTCAGATCGACCGGTTTGA
ATTTCCGGTGCTTCTGGTTCTCGCCACGCTCGGCATGCTGCTGATGATTTCGGCCAACGATCTCATCTCGCTCTACCTGT
CGCTGGAACTGCAGTCGCTTGCGCTTTACGTCGTGGCTGCGATCAACCGCGACAGCGTCCGGTCGACCGAAGCGGGTCTG
AAATATTTCGTTCTCGGCGCACTCTCTTCGGGGATGCTGCTTTACGGCATGTCGCTCGTCTACGGCTTCACCGGCCATAC
AGGGTTTGACGAGATTGCGGCGGCGCTGACCGCCGAGGGCCGTTCTCTCGGCCTCGTCTTCGGGCTGGTGTTCATCCTTG
CGGGCCTCGCTTTCAAGATCTCCGCCGTGCCGTTTCATATGTGGACGCCTGACGTCTACGAGGGCGCCCCGACGCCGGTG
ACGGCATTCTTTGCCGCCGGACCCAAGATCGCGGCCATCTCAATCCTCGTTCGCATCGTCATCAATGCCTTCGAACCCGT
CGTCGCCGACTGGCAGCAGATCGTCGTCTTCATTTCGATCGCTTCGATGCTGCTCGGCTCCTTCGCCGCGATCGGTCAGC
GCAACATCAAGCGGCTGATGGCGTACTCGTCGATCGGCCATATGGGTTATGCGCTGGTCGGCCTTGCTGCCGGTTCGATG
GCCGGCGTACGCGGCGTGATCCTTTATATGCTGATCTACATGGTCATGACGTTGGGCACCTTCGCCTGCATCCTCGCCAT
GCGCCGCAGGGAGGGCGAGCATGTCGAGGGCATCGACGATCTCGCCGGTCTTTCGCAGACCAACCCTTTCATGGCGACGG
TGCTGACGATCCTCATGTTCTCGCTTGCCGGCATCCCGCCACTTGCAGGGTTCTTCGGTAAGTACTTCGTCTTCGTGGCC
GCGATCGAGGCGCATCTCTATGCATTGGCGATTATCGGCGTGCTCGCTTCCGTCGTCGGCGCTTACTATTATCTGCGGGT
CATCAAGGTGATGTGGTTCGAGGAGCCCAGAGGCGAGTTTGCCCGGACGGCGGGTGAGCTTCGGCTGGTGTTCGGCCTGT
CGGGCCTCTTCGTGCTCGGCTATGTGCTCATCGGCGGCCCGCTTGGAAGCGCGGCCGAAGCTGCGGCACGGACCTTCTTT
TGA

Upstream 100 bases:

>100_bases
GCGGCCTCCGTGGACCTGCTCGTCAACAACTACGCCGCCGCCCTGCAGGCAGCGCAAGACGTTGCACTTTCGGTGCAATG
ATCAAGGGACGTGATGTGAC

Downstream 100 bases:

>100_bases
GTGGCGGGCAGGGCAGCGGCCGGAATTCGCTTGAACAGTTCCGGCACGTCGCGCTCGCCGAAGCGGTTTCGACCAACAGC
GAGTGCCTTTTGCGGGCGCG

Product: NADH dehydrogenase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N 1; NDH-1 subunit N 1 [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MTAETLIASLQLSMPELILAVGAMALLMIGVFSSERATPTVTGLAVAVLIIAGLWLVLKTGEGAAYGGAFLSDPFAKFMK
VLALIGSITVMVMTVGHARSAQIDRFEFPVLLVLATLGMLLMISANDLISLYLSLELQSLALYVVAAINRDSVRSTEAGL
KYFVLGALSSGMLLYGMSLVYGFTGHTGFDEIAAALTAEGRSLGLVFGLVFILAGLAFKISAVPFHMWTPDVYEGAPTPV
TAFFAAGPKIAAISILVRIVINAFEPVVADWQQIVVFISIASMLLGSFAAIGQRNIKRLMAYSSIGHMGYALVGLAAGSM
AGVRGVILYMLIYMVMTLGTFACILAMRRREGEHVEGIDDLAGLSQTNPFMATVLTILMFSLAGIPPLAGFFGKYFVFVA
AIEAHLYALAIIGVLASVVGAYYYLRVIKVMWFEEPRGEFARTAGELRLVFGLSGLFVLGYVLIGGPLGSAAEAAARTFF

Sequences:

>Translated_480_residues
MTAETLIASLQLSMPELILAVGAMALLMIGVFSSERATPTVTGLAVAVLIIAGLWLVLKTGEGAAYGGAFLSDPFAKFMK
VLALIGSITVMVMTVGHARSAQIDRFEFPVLLVLATLGMLLMISANDLISLYLSLELQSLALYVVAAINRDSVRSTEAGL
KYFVLGALSSGMLLYGMSLVYGFTGHTGFDEIAAALTAEGRSLGLVFGLVFILAGLAFKISAVPFHMWTPDVYEGAPTPV
TAFFAAGPKIAAISILVRIVINAFEPVVADWQQIVVFISIASMLLGSFAAIGQRNIKRLMAYSSIGHMGYALVGLAAGSM
AGVRGVILYMLIYMVMTLGTFACILAMRRREGEHVEGIDDLAGLSQTNPFMATVLTILMFSLAGIPPLAGFFGKYFVFVA
AIEAHLYALAIIGVLASVVGAYYYLRVIKVMWFEEPRGEFARTAGELRLVFGLSGLFVLGYVLIGGPLGSAAEAAARTFF
>Mature_479_residues
TAETLIASLQLSMPELILAVGAMALLMIGVFSSERATPTVTGLAVAVLIIAGLWLVLKTGEGAAYGGAFLSDPFAKFMKV
LALIGSITVMVMTVGHARSAQIDRFEFPVLLVLATLGMLLMISANDLISLYLSLELQSLALYVVAAINRDSVRSTEAGLK
YFVLGALSSGMLLYGMSLVYGFTGHTGFDEIAAALTAEGRSLGLVFGLVFILAGLAFKISAVPFHMWTPDVYEGAPTPVT
AFFAAGPKIAAISILVRIVINAFEPVVADWQQIVVFISIASMLLGSFAAIGQRNIKRLMAYSSIGHMGYALVGLAAGSMA
GVRGVILYMLIYMVMTLGTFACILAMRRREGEHVEGIDDLAGLSQTNPFMATVLTILMFSLAGIPPLAGFFGKYFVFVAA
IEAHLYALAIIGVLASVVGAYYYLRVIKVMWFEEPRGEFARTAGELRLVFGLSGLFVLGYVLIGGPLGSAAEAAARTFF

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Homo sapiens, GI251831108, Length=301, Percent_Identity=23.9202657807309, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI251831117, Length=412, Percent_Identity=24.5145631067961, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI145693160, Length=419, Percent_Identity=36.7541766109785, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1788831, Length=394, Percent_Identity=25.3807106598985, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788613, Length=328, Percent_Identity=23.4756097560976, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788614, Length=317, Percent_Identity=24.9211356466877, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788827, Length=380, Percent_Identity=25, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788829, Length=283, Percent_Identity=25.4416961130742, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 50993; Mature: 50861

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAETLIASLQLSMPELILAVGAMALLMIGVFSSERATPTVTGLAVAVLIIAGLWLVLKT
CCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
GEGAAYGGAFLSDPFAKFMKVLALIGSITVMVMTVGHARSAQIDRFEFPVLLVLATLGML
CCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
LMISANDLISLYLSLELQSLALYVVAAINRDSVRSTEAGLKYFVLGALSSGMLLYGMSLV
HHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
YGFTGHTGFDEIAAALTAEGRSLGLVFGLVFILAGLAFKISAVPFHMWTPDVYEGAPTPV
HHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHCCCCCCCH
TAFFAAGPKIAAISILVRIVINAFEPVVADWQQIVVFISIASMLLGSFAAIGQRNIKRLM
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYSSIGHMGYALVGLAAGSMAGVRGVILYMLIYMVMTLGTFACILAMRRREGEHVEGIDD
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LAGLSQTNPFMATVLTILMFSLAGIPPLAGFFGKYFVFVAAIEAHLYALAIIGVLASVVG
HCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYYYLRVIKVMWFEEPRGEFARTAGELRLVFGLSGLFVLGYVLIGGPLGSAAEAAARTFF
HHHHHHHHHHHHHCCCCHHHHHHHHCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
TAETLIASLQLSMPELILAVGAMALLMIGVFSSERATPTVTGLAVAVLIIAGLWLVLKT
CHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
GEGAAYGGAFLSDPFAKFMKVLALIGSITVMVMTVGHARSAQIDRFEFPVLLVLATLGML
CCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
LMISANDLISLYLSLELQSLALYVVAAINRDSVRSTEAGLKYFVLGALSSGMLLYGMSLV
HHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
YGFTGHTGFDEIAAALTAEGRSLGLVFGLVFILAGLAFKISAVPFHMWTPDVYEGAPTPV
HHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHCCCCCCCH
TAFFAAGPKIAAISILVRIVINAFEPVVADWQQIVVFISIASMLLGSFAAIGQRNIKRLM
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYSSIGHMGYALVGLAAGSMAGVRGVILYMLIYMVMTLGTFACILAMRRREGEHVEGIDD
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LAGLSQTNPFMATVLTILMFSLAGIPPLAGFFGKYFVFVAAIEAHLYALAIIGVLASVVG
HCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYYYLRVIKVMWFEEPRGEFARTAGELRLVFGLSGLFVLGYVLIGGPLGSAAEAAARTFF
HHHHHHHHHHHHHCCCCHHHHHHHHCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]