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The map label for this gene is nqo12 [H]

Identifier: 150396124

GI number: 150396124

Start: 971211

End: 973205

Strand: Direct

Name: nqo12 [H]

Synonym: Smed_0901

Alternate gene names: 150396124

Gene position: 971211-973205 (Clockwise)

Preceding gene: 150396123

Following gene: 150396125

Centisome position: 25.68

GC content: 60.45

Gene sequence:

>1995_bases
ATGGACACCATCGTCAAAGCTATCGTCTTCCTGCCTCTGATCGGCTTCCTGATCGCCGGCCTTCTCGGCACGCAGATCGG
CGCCAAGGCATCCGAATATGTGACCTGCGGCCTGATGCTCGTCACAGCCGCGCTCTCCTGGTTCGTCTTTTTCCATGTCG
CCCTCGGCGAGGAGGAGATGATCAGTGTTTCGGTGCTGCGCTGGATCCAGTCCGGAGGCTTCGATGTCGATTGGGCTTTC
CGCGTCGACACGCTGACCGCGGTCATGTTCGTGGTCGTCAACACCGTTTCGACCCTGGTGCACATCTATTCGATCGGATA
CATGCACCACGATCCGAACCGCCCGCGCTTCTTTGCTTATCTGTCGCTCTTCACGTTCGCGATGCTGATGCTCATCACGT
CGGACAATCTGCTGCAGATGTTCTTTGGCTGGGAAGGCGTTGGCGTCGCGTCATACCTGCTGATCGGCTTCTGGTACAAG
AAGCCCTCCGCCAATGCAGCGGCAATGAAGGCCTTCATCGTAAACCGCGTCGGTGACTTCGGTTTCGCGCTCGGCATCTT
CTGCGTCTTCGTCCTTTTCGGCTCGATCAATTTTGAGACCATCTTTGCCGCCGCTCGGAACTATCTGCCGGCCGAGGGTG
CTGCGGCAGGGGACGTGGTCATCAATCTGTTCGGAATGCAGCTCGACAAGGGACATGCGTTGACGGCCACCTGCCTGCTG
CTCTTCATGGGCGCGATGGGCAAATCGGCGCAGTTCCTGCTGCATACCTGGCTGCCGGACGCCATGGAAGGTCCGACTCC
GGTTTCGGCGCTTATCCATGCCGCGACCATGGTTACAGCCGGGGTCTTCCTCGTCGCCCGCATGTCGCCGCTCTTCGAGC
TGTCGCCTGATGCCCTGACTTTCGTTACCCTGATCGGCGCAATCACCGCATTCTTCGCGGCGACGGTCGGCCTTGTTCAG
AACGACATCAAGCGCGTCATCGCCTATTCGACCTGCTCGCAGCTCGGCTACATGTTCGTCGCACTCGGCGTCGGCGCTTA
CGGCGCCGCGATCTTCCATCTCTTCACGCACGCCTTCTTCAAGGCGCTCCTGTTCCTCGGCGCCGGCTCCGTCATCCATG
CCGTCGACGGTGAGCAGGACATGCGTCACATGGGCGGGCTGCGCACGCATATTCCGGTCACCTACTGGATGATGTTCATC
GGCACGATCGCGCTGACCGGCGTCGGCATTCCCGGCACTGTCATCGGCACGGCCGGCTTCTTCTCGAAGGATGCGATCAT
CGAATCCGCGTTCGCGTCGCACAGCGTCGTTTCCGGCTTCGCCTTCGTGCTGCTGGTCATTGCGGCGCTCTTCACCAGCT
TCTACTCCTGGCGCCTGACCTTCATGACGTTCCACGGCAAGCCGCGCGCTTCCTCGGATGTCATGCATCACGTCCACGAG
TCGCCCCAAGTGATGCTGGTGCCGCTCTACATCCTGGCCGCCGGCGCCCTCGTTGCGGGTTTCCTGTTCCACGACTATTT
CTTCGGCCATCACTATGCCGAGTTCTGGCAGGGCTCGTTGTTCACGTCGGCCGAAAACGAACTCCTGGAGGAATTTCATC
ACGTTCCGCTGTGGGTGAAATGGAGCCCGTTCGCGGCCATGGCGCTTGGCCTGCTTACGGCCTGGTACATGTACATCCGC
TCGCCGGAGACGCCCAAATACCTCGCCGAGCAGCATCGAGGACTTTATCAGTTCCTGCTCAACAAGTGGTATTTCGACGA
ACTCTACGACTTCCTGTTCGTCCGCACCGCCAAGCGCCTCGGCACCTTCCTCTGGAAGGAGGGTGATGGCCGGGTGATCG
ACGGATACGGCCCGAACGGGATCGCCGCGCGCGTTCTCGACGTGACCGACCGGGTTGTCCGCCTGCAGACCGGATACCTT
TACCACTACGCGTTCGCCATGCTGATCGGCATCGCGGCGCTCGTTACATGGATGATGCTCGGGAGTTCCTTCTGA

Upstream 100 bases:

>100_bases
GCCGAGGCTGCCATCGGACTTGCAATTCTCGTCGTCTTCTATCGGAACCGCGGCTCGATTGCCGTCGAAGACGTCAACAT
GATGAAGGGCTGACAGGGCT

Downstream 100 bases:

>100_bases
TGACCGATTGGCCCGTACTTTCCGCGGTCACCTTCATGCCGCTCGTCGGCGTCCTGCTTCTCTTGCTGACAAGAGAAGAC
AGCGCCTACGGCCGCCGCAA

Product: NADH dehydrogenase subunit L

Products: NA

Alternate protein names: NADH dehydrogenase I, chain 12; NDH-1, chain 12 [H]

Number of amino acids: Translated: 664; Mature: 664

Protein sequence:

>664_residues
MDTIVKAIVFLPLIGFLIAGLLGTQIGAKASEYVTCGLMLVTAALSWFVFFHVALGEEEMISVSVLRWIQSGGFDVDWAF
RVDTLTAVMFVVVNTVSTLVHIYSIGYMHHDPNRPRFFAYLSLFTFAMLMLITSDNLLQMFFGWEGVGVASYLLIGFWYK
KPSANAAAMKAFIVNRVGDFGFALGIFCVFVLFGSINFETIFAAARNYLPAEGAAAGDVVINLFGMQLDKGHALTATCLL
LFMGAMGKSAQFLLHTWLPDAMEGPTPVSALIHAATMVTAGVFLVARMSPLFELSPDALTFVTLIGAITAFFAATVGLVQ
NDIKRVIAYSTCSQLGYMFVALGVGAYGAAIFHLFTHAFFKALLFLGAGSVIHAVDGEQDMRHMGGLRTHIPVTYWMMFI
GTIALTGVGIPGTVIGTAGFFSKDAIIESAFASHSVVSGFAFVLLVIAALFTSFYSWRLTFMTFHGKPRASSDVMHHVHE
SPQVMLVPLYILAAGALVAGFLFHDYFFGHHYAEFWQGSLFTSAENELLEEFHHVPLWVKWSPFAAMALGLLTAWYMYIR
SPETPKYLAEQHRGLYQFLLNKWYFDELYDFLFVRTAKRLGTFLWKEGDGRVIDGYGPNGIAARVLDVTDRVVRLQTGYL
YHYAFAMLIGIAALVTWMMLGSSF

Sequences:

>Translated_664_residues
MDTIVKAIVFLPLIGFLIAGLLGTQIGAKASEYVTCGLMLVTAALSWFVFFHVALGEEEMISVSVLRWIQSGGFDVDWAF
RVDTLTAVMFVVVNTVSTLVHIYSIGYMHHDPNRPRFFAYLSLFTFAMLMLITSDNLLQMFFGWEGVGVASYLLIGFWYK
KPSANAAAMKAFIVNRVGDFGFALGIFCVFVLFGSINFETIFAAARNYLPAEGAAAGDVVINLFGMQLDKGHALTATCLL
LFMGAMGKSAQFLLHTWLPDAMEGPTPVSALIHAATMVTAGVFLVARMSPLFELSPDALTFVTLIGAITAFFAATVGLVQ
NDIKRVIAYSTCSQLGYMFVALGVGAYGAAIFHLFTHAFFKALLFLGAGSVIHAVDGEQDMRHMGGLRTHIPVTYWMMFI
GTIALTGVGIPGTVIGTAGFFSKDAIIESAFASHSVVSGFAFVLLVIAALFTSFYSWRLTFMTFHGKPRASSDVMHHVHE
SPQVMLVPLYILAAGALVAGFLFHDYFFGHHYAEFWQGSLFTSAENELLEEFHHVPLWVKWSPFAAMALGLLTAWYMYIR
SPETPKYLAEQHRGLYQFLLNKWYFDELYDFLFVRTAKRLGTFLWKEGDGRVIDGYGPNGIAARVLDVTDRVVRLQTGYL
YHYAFAMLIGIAALVTWMMLGSSF
>Mature_664_residues
MDTIVKAIVFLPLIGFLIAGLLGTQIGAKASEYVTCGLMLVTAALSWFVFFHVALGEEEMISVSVLRWIQSGGFDVDWAF
RVDTLTAVMFVVVNTVSTLVHIYSIGYMHHDPNRPRFFAYLSLFTFAMLMLITSDNLLQMFFGWEGVGVASYLLIGFWYK
KPSANAAAMKAFIVNRVGDFGFALGIFCVFVLFGSINFETIFAAARNYLPAEGAAAGDVVINLFGMQLDKGHALTATCLL
LFMGAMGKSAQFLLHTWLPDAMEGPTPVSALIHAATMVTAGVFLVARMSPLFELSPDALTFVTLIGAITAFFAATVGLVQ
NDIKRVIAYSTCSQLGYMFVALGVGAYGAAIFHLFTHAFFKALLFLGAGSVIHAVDGEQDMRHMGGLRTHIPVTYWMMFI
GTIALTGVGIPGTVIGTAGFFSKDAIIESAFASHSVVSGFAFVLLVIAALFTSFYSWRLTFMTFHGKPRASSDVMHHVHE
SPQVMLVPLYILAAGALVAGFLFHDYFFGHHYAEFWQGSLFTSAENELLEEFHHVPLWVKWSPFAAMALGLLTAWYMYIR
SPETPKYLAEQHRGLYQFLLNKWYFDELYDFLFVRTAKRLGTFLWKEGDGRVIDGYGPNGIAARVLDVTDRVVRLQTGYL
YHYAFAMLIGIAALVTWMMLGSSF

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=498, Percent_Identity=39.3574297188755, Blast_Score=317, Evalue=2e-86,
Organism=Escherichia coli, GI1788614, Length=592, Percent_Identity=40.0337837837838, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1788829, Length=366, Percent_Identity=32.7868852459016, Blast_Score=135, Evalue=9e-33,
Organism=Escherichia coli, GI1788831, Length=348, Percent_Identity=31.8965517241379, Blast_Score=119, Evalue=8e-28,
Organism=Escherichia coli, GI1788827, Length=447, Percent_Identity=27.9642058165548, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1788613, Length=491, Percent_Identity=24.4399185336049, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI2367154, Length=215, Percent_Identity=28.3720930232558, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI145693160, Length=280, Percent_Identity=25, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 73315; Mature: 73315

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCC
>Mature Secondary Structure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CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]