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The map label for this gene is ligE [H]

Identifier: 150395998

GI number: 150395998

Start: 834066

End: 834755

Strand: Direct

Name: ligE [H]

Synonym: Smed_0774

Alternate gene names: 150395998

Gene position: 834066-834755 (Clockwise)

Preceding gene: 150395996

Following gene: 150395999

Centisome position: 22.05

GC content: 59.71

Gene sequence:

>690_bases
ATGACCCGCAAACTCTACGCACTCTGTGGAACCGACAAGAGCCGCCCGTTTTCGCCGCATGTGTGGAAGGCGAAGCTTTC
GCTAGCGCATAAGGGGTTGACCTTCGACGTTGCACCCGTCGGCTTTACCGCGATTCCGAGTCTAGAGGAGGGGGCTACAA
AGATCGTTCCCCTGCTGCGCGATGGAGAAAAGCTGGTCAACGACAGCTTCGATATCGCGCTCTATCTTGAAGAGACCTAT
CCCGATCGTCCGTCCCTGTTTGCCGGTGAAGGCGGTAAGGCCATGGCACGCTTCGTCGAGAGCTGGTCGCAAACTGCCCT
GCATACGGCGATCGGGCGCATCGCTATCATGGACATCCATGACAGCCTCGATCCCGTCGACCAAGCCTATTTCCGGGCAA
GCCGCGAGCAACGGTTCGGCAAATCACTGGAGACGGTTGCCGGAGCCGGGCGGGAAGATCTCGAAATCTTCTCGGCGAAG
CTCGAACCGCTTCGCCACATGTTGAAGTCCCAGCCCTTCCTTGGTGGGAACAGCCCGCTCTTTGCAGATTATATCGTTTT
CGGCGCGCTCCAATGGGCACGCATCGTTTCGCCGCAGCGCCTGCTTTCCGAAGGCGATGTCGTTACCGACTGGTTCGAGC
GGTGCCTCGAGCTTCACGGCGGTCTTGGCCGCAGCGTGACGGCGGCGTGA

Upstream 100 bases:

>100_bases
AGCGACCTTTGTGCGCCTGATAAGGCGCAAGGCGCTGTAGATGTAACCCGCGAGCGGCGTTTTCCCTTGTGCTGCTCGGA
AGCGAAATTCAGGAGCCTGC

Downstream 100 bases:

>100_bases
AACTGAGGCGCAAGCGGCCGAATCGTGGCCACGCCCCCTTGTTTTGCACGGAATTGGCGGCTAATGAACCGCCACTTCTC
AAGAGCAAGGAATTGAGCCC

Product: glutathione S-transferase domain-containing protein

Products: NA

Alternate protein names: Beta-aryl ether cleaving enzyme [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTRKLYALCGTDKSRPFSPHVWKAKLSLAHKGLTFDVAPVGFTAIPSLEEGATKIVPLLRDGEKLVNDSFDIALYLEETY
PDRPSLFAGEGGKAMARFVESWSQTALHTAIGRIAIMDIHDSLDPVDQAYFRASREQRFGKSLETVAGAGREDLEIFSAK
LEPLRHMLKSQPFLGGNSPLFADYIVFGALQWARIVSPQRLLSEGDVVTDWFERCLELHGGLGRSVTAA

Sequences:

>Translated_229_residues
MTRKLYALCGTDKSRPFSPHVWKAKLSLAHKGLTFDVAPVGFTAIPSLEEGATKIVPLLRDGEKLVNDSFDIALYLEETY
PDRPSLFAGEGGKAMARFVESWSQTALHTAIGRIAIMDIHDSLDPVDQAYFRASREQRFGKSLETVAGAGREDLEIFSAK
LEPLRHMLKSQPFLGGNSPLFADYIVFGALQWARIVSPQRLLSEGDVVTDWFERCLELHGGLGRSVTAA
>Mature_228_residues
TRKLYALCGTDKSRPFSPHVWKAKLSLAHKGLTFDVAPVGFTAIPSLEEGATKIVPLLRDGEKLVNDSFDIALYLEETYP
DRPSLFAGEGGKAMARFVESWSQTALHTAIGRIAIMDIHDSLDPVDQAYFRASREQRFGKSLETVAGAGREDLEIFSAKL
EPLRHMLKSQPFLGGNSPLFADYIVFGALQWARIVSPQRLLSEGDVVTDWFERCLELHGGLGRSVTAA

Specific function: Able to degrade various dimeric lignin compounds. Catalyzes the unique and reductive cleavage of arylglycerol-beta- aryl ether [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 25296; Mature: 25165

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKLYALCGTDKSRPFSPHVWKAKLSLAHKGLTFDVAPVGFTAIPSLEEGATKIVPLLR
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCEECCCCCCCHHHHHHHHH
DGEKLVNDSFDIALYLEETYPDRPSLFAGEGGKAMARFVESWSQTALHTAIGRIAIMDIH
CCHHHHCCCCEEEEEEECCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECC
DSLDPVDQAYFRASREQRFGKSLETVAGAGREDLEIFSAKLEPLRHMLKSQPFLGGNSPL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
FADYIVFGALQWARIVSPQRLLSEGDVVTDWFERCLELHGGLGRSVTAA
HHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TRKLYALCGTDKSRPFSPHVWKAKLSLAHKGLTFDVAPVGFTAIPSLEEGATKIVPLLR
CCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCEECCCCCCCHHHHHHHHH
DGEKLVNDSFDIALYLEETYPDRPSLFAGEGGKAMARFVESWSQTALHTAIGRIAIMDIH
CCHHHHCCCCEEEEEEECCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECC
DSLDPVDQAYFRASREQRFGKSLETVAGAGREDLEIFSAKLEPLRHMLKSQPFLGGNSPL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
FADYIVFGALQWARIVSPQRLLSEGDVVTDWFERCLELHGGLGRSVTAA
HHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1744051; 8495726 [H]