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The map label for this gene is lcfB [H]

Identifier: 150395996

GI number: 150395996

Start: 830628

End: 833291

Strand: Direct

Name: lcfB [H]

Synonym: Smed_0772

Alternate gene names: 150395996

Gene position: 830628-833291 (Clockwise)

Preceding gene: 150395995

Following gene: 150395998

Centisome position: 21.96

GC content: 60.4

Gene sequence:

>2664_bases
ATGGAGGTGGTGGCTGTGGACGTAGATTCTTCGATGTCGCCGATGAGTGTCGCAAGTCTGGCCGCGCGGGGCGAGAGGCC
GGCGCTGCTGCTGCGCGGCGGGAGCATCATCACCTATCGCGAACTGGCGCAGCGCGTCGGCCGCCTTGCATCGCAATGGC
GCGGCGGCCGCGGTCTCGTTATGCTCGAGGCCGAGCTTTCCGATCACTCTATCGTCGGCTATCTCGCCGCGCTCGAAGCG
GGGCACGCGGTTGCCCTGTGCGCCGGCGCATCGATGGTCACGGCTCGTGAAGTTTTCAATCCGGAATACTGCTATCGCCG
TTGCGACGGCCGCTGGCGTATGGAACGCCTGGACACAGATCCGGTCGATCCACACCCGCAACTCGCCGTCCTGCTCTCGA
CTTCCGGGAGTACCGGCTATGGCAAATGCGTGCGGCTGTCTTATGCGAACATTCAGTCCAACGCACTGTCTATCGCCGGA
TATCTCGGCTTGAGCGCATCGGACCGAGGCTGTTTGACCCTGCCGCTTCATTATTCCTACGGACTTTCGATTCTGAACAC
TCACCTGTCTGTGGGCGCGAGCGTCTATCTGCCCGGCAGTTCAATTTCAGACGACGGCTTCCTCGACGATCTTGCGGAGA
GCGGCAGTTCCAGTTTCACCGGCGTACCCTATTCCTATGACCTCTTGGAAAAGGTGGGTTTCCGCGAACGCGATTTCCCT
AAGCTTCGCTTCATGACGGTCGCCGGAGGACGAATGTCGCCGGAACTCGTCCGCCGTTACGATGAACATCTTTCGGCGCG
CGGCGCGTCTTTTTTCGTGATGTATGGTCAAACCGAGGCAACCGCCCGGATGGCCTACATGCCGCCGGAAAGTCTGCGAG
GCAGAGAGGATCGCATCGGCATCGCCATACCGGGCGGCAGCCTGACGATCGTCGACGAAGAAGGCAGGACCATCCAGGCC
GCCGATCAGGCGGGCGAACTCGTCTATCGCGGGCCGAACGTGATGATGGGCTATGCCTCGTCGCGCGCCGATCTCGCAAG
GGGTGCCGAGTTGTCGGAACTGCGGACCGGCGATCTCGCCTCCAGGGATGCCGAAGGCTATTTCCGGATCGTCGGACGAA
CGAAGCGGCTTTCGAAAATTGCCGGTCTACGCATAGGTCATGACAGCCTGGAAGCCGCCCTCGAACGGCATGGCATCTCT
GCTGCCGTCGTCGGCAACGATACCGAGGTCCATGCTTATTATGCTGGGGATCGCGATCCGGAAGAAGTTTGCCGGCTGCT
CTTGCAGGCGAGCGGCTTGACGGCCATGCAGGTCGGGGCTTCACGCTTGAATTGCCTTCCGAGGCTGGTATCGGGAAAAA
TCGACTATCAGAGCCTTGCCGTTCCTCCGGCCGCAACGCAGTGTCGCCGTTTCGAGCGGCAGAGCGTGGAGACTGTTTTC
GCCCGGATATTCTATCCGAAGAAGATACGGTCGGAGGATAGTTTTCTGTCGCTCGGCGGCGACTCGCTCCGTTTCGTCCA
GCTTTCAATCGGACTTGAAAAAATCCTCGGAGAACTCCCTGAATCATGGGAGAAATTGCCGATCGGAGAGCTGGCGGCGC
GCAGCAGCCGAAAGGGCAAGATCGGGCAGATCGCAACCGACCTCCTGATCCGGGCCCTGGCGATCATCCTTGTCGTGCTC
CATCATGAGACGCTTTGGCCGATTCCGGGTGGTTCGGCTGCAATGGTCATTCTTGCCGGATTCAGTCTTGCGCGGTTTCA
AAGCGGGGCATTGTTCTCCGGCGCGATAGACAAGCTCCTGTGGCCCCTGGCACAGGTCCTCATTCCATATTACCTCATCG
TAGCGGCTTACGCGCTCGCCTGGGGCGAAGTGCCCTGGGCCTCGATCTTTCTCGTCGGCAATTTCGCTTTTGCCGATCCT
GAGCGGCACAGCATGGTGCCCTATCTCTATTGGTTCATCGAAGCCTATAGCCAGATGCTGCTGGTGTTCGGCGCTCTGTT
CGCCGTCCCCTCCATTCGACGGGCGGCGGCAAAGAGGCCTTTCGCCGCGGGCATGATCCTGTTCGTGGCGGCATTCGCGG
CGCGAATGGCCTTTCCACTCCTCGTCGAGATCGGCAATCGCCAGGTATTCACCCTTCCATGGATCTTCTACATGGCAGTC
CTGGGCTGGTGTGCGGCCATAGCCGCAACGTGGAAAGAGAAATCGGCTGTGATGCTCGCGGGAACGGGTGTTTTCCTGTT
TCTAGGACTTTACGAGGGCGTGTGGATTGGTACGAAGATCAAATATCTGCTGCAGATCATTGTTCTGGCAGCACTGCTCT
TCCTGCCGCGCGTCCGCCTGCCGCAATGGTCAGCGCGCCTGATCCTGCCGCTTTCTGCCGCAAGTTTCCATATCTACATA
CTGCACCGTTTCGTGCCGGAACTGCTGCTGCAACCGATGCAATCCTTGACGTCGCCCGTCGTCTTCGCCGTTGCCTCTAT
TCTTGGCGGCATAGCCCTCGGGGCAGCGGCCTGGCTGGTGCAGCGACAGCTGGTGGCCTGGCTGGCTGCACGGGAGAGCC
GTATCTTGCTCGCCCCGCGAGACGGGCCCGGCAGCGGTCTCGCGTCAGGCAGCCCACTTTTTGCCGTCGGTCCGCAGGAA
GAATACCAGATCCAGCGTCAATGA

Upstream 100 bases:

>100_bases
CTGACCACCGTCAGGCCGCGCTCGCTGTCGCAGTTGCCGTTTCGGGCTGCGTTTTGATACTTGACGCCCAAACTCAGCAA
TAATATTCCGCCTCGCTAAA

Downstream 100 bases:

>100_bases
CGGCCGCTCGAGCGCCGTCAAGATCGCGTGGGACTGGCCCCGGTGATGGGTCTGATGGTTGAAGAAATGCGCGAGCGTTC
GCCCGAGTCTTTCGGTTACC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 887; Mature: 887

Protein sequence:

>887_residues
MEVVAVDVDSSMSPMSVASLAARGERPALLLRGGSIITYRELAQRVGRLASQWRGGRGLVMLEAELSDHSIVGYLAALEA
GHAVALCAGASMVTAREVFNPEYCYRRCDGRWRMERLDTDPVDPHPQLAVLLSTSGSTGYGKCVRLSYANIQSNALSIAG
YLGLSASDRGCLTLPLHYSYGLSILNTHLSVGASVYLPGSSISDDGFLDDLAESGSSSFTGVPYSYDLLEKVGFRERDFP
KLRFMTVAGGRMSPELVRRYDEHLSARGASFFVMYGQTEATARMAYMPPESLRGREDRIGIAIPGGSLTIVDEEGRTIQA
ADQAGELVYRGPNVMMGYASSRADLARGAELSELRTGDLASRDAEGYFRIVGRTKRLSKIAGLRIGHDSLEAALERHGIS
AAVVGNDTEVHAYYAGDRDPEEVCRLLLQASGLTAMQVGASRLNCLPRLVSGKIDYQSLAVPPAATQCRRFERQSVETVF
ARIFYPKKIRSEDSFLSLGGDSLRFVQLSIGLEKILGELPESWEKLPIGELAARSSRKGKIGQIATDLLIRALAIILVVL
HHETLWPIPGGSAAMVILAGFSLARFQSGALFSGAIDKLLWPLAQVLIPYYLIVAAYALAWGEVPWASIFLVGNFAFADP
ERHSMVPYLYWFIEAYSQMLLVFGALFAVPSIRRAAAKRPFAAGMILFVAAFAARMAFPLLVEIGNRQVFTLPWIFYMAV
LGWCAAIAATWKEKSAVMLAGTGVFLFLGLYEGVWIGTKIKYLLQIIVLAALLFLPRVRLPQWSARLILPLSAASFHIYI
LHRFVPELLLQPMQSLTSPVVFAVASILGGIALGAAAWLVQRQLVAWLAARESRILLAPRDGPGSGLASGSPLFAVGPQE
EYQIQRQ

Sequences:

>Translated_887_residues
MEVVAVDVDSSMSPMSVASLAARGERPALLLRGGSIITYRELAQRVGRLASQWRGGRGLVMLEAELSDHSIVGYLAALEA
GHAVALCAGASMVTAREVFNPEYCYRRCDGRWRMERLDTDPVDPHPQLAVLLSTSGSTGYGKCVRLSYANIQSNALSIAG
YLGLSASDRGCLTLPLHYSYGLSILNTHLSVGASVYLPGSSISDDGFLDDLAESGSSSFTGVPYSYDLLEKVGFRERDFP
KLRFMTVAGGRMSPELVRRYDEHLSARGASFFVMYGQTEATARMAYMPPESLRGREDRIGIAIPGGSLTIVDEEGRTIQA
ADQAGELVYRGPNVMMGYASSRADLARGAELSELRTGDLASRDAEGYFRIVGRTKRLSKIAGLRIGHDSLEAALERHGIS
AAVVGNDTEVHAYYAGDRDPEEVCRLLLQASGLTAMQVGASRLNCLPRLVSGKIDYQSLAVPPAATQCRRFERQSVETVF
ARIFYPKKIRSEDSFLSLGGDSLRFVQLSIGLEKILGELPESWEKLPIGELAARSSRKGKIGQIATDLLIRALAIILVVL
HHETLWPIPGGSAAMVILAGFSLARFQSGALFSGAIDKLLWPLAQVLIPYYLIVAAYALAWGEVPWASIFLVGNFAFADP
ERHSMVPYLYWFIEAYSQMLLVFGALFAVPSIRRAAAKRPFAAGMILFVAAFAARMAFPLLVEIGNRQVFTLPWIFYMAV
LGWCAAIAATWKEKSAVMLAGTGVFLFLGLYEGVWIGTKIKYLLQIIVLAALLFLPRVRLPQWSARLILPLSAASFHIYI
LHRFVPELLLQPMQSLTSPVVFAVASILGGIALGAAAWLVQRQLVAWLAARESRILLAPRDGPGSGLASGSPLFAVGPQE
EYQIQRQ
>Mature_887_residues
MEVVAVDVDSSMSPMSVASLAARGERPALLLRGGSIITYRELAQRVGRLASQWRGGRGLVMLEAELSDHSIVGYLAALEA
GHAVALCAGASMVTAREVFNPEYCYRRCDGRWRMERLDTDPVDPHPQLAVLLSTSGSTGYGKCVRLSYANIQSNALSIAG
YLGLSASDRGCLTLPLHYSYGLSILNTHLSVGASVYLPGSSISDDGFLDDLAESGSSSFTGVPYSYDLLEKVGFRERDFP
KLRFMTVAGGRMSPELVRRYDEHLSARGASFFVMYGQTEATARMAYMPPESLRGREDRIGIAIPGGSLTIVDEEGRTIQA
ADQAGELVYRGPNVMMGYASSRADLARGAELSELRTGDLASRDAEGYFRIVGRTKRLSKIAGLRIGHDSLEAALERHGIS
AAVVGNDTEVHAYYAGDRDPEEVCRLLLQASGLTAMQVGASRLNCLPRLVSGKIDYQSLAVPPAATQCRRFERQSVETVF
ARIFYPKKIRSEDSFLSLGGDSLRFVQLSIGLEKILGELPESWEKLPIGELAARSSRKGKIGQIATDLLIRALAIILVVL
HHETLWPIPGGSAAMVILAGFSLARFQSGALFSGAIDKLLWPLAQVLIPYYLIVAAYALAWGEVPWASIFLVGNFAFADP
ERHSMVPYLYWFIEAYSQMLLVFGALFAVPSIRRAAAKRPFAAGMILFVAAFAARMAFPLLVEIGNRQVFTLPWIFYMAV
LGWCAAIAATWKEKSAVMLAGTGVFLFLGLYEGVWIGTKIKYLLQIIVLAALLFLPRVRLPQWSARLILPLSAASFHIYI
LHRFVPELLLQPMQSLTSPVVFAVASILGGIALGAAAWLVQRQLVAWLAARESRILLAPRDGPGSGLASGSPLFAVGPQE
EYQIQRQ

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=302, Percent_Identity=26.4900662251656, Blast_Score=85, Evalue=4e-16,
Organism=Homo sapiens, GI157311624, Length=387, Percent_Identity=22.9974160206718, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI157311622, Length=387, Percent_Identity=22.9974160206718, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI58082049, Length=387, Percent_Identity=22.9974160206718, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI145693145, Length=275, Percent_Identity=30.1818181818182, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1788107, Length=277, Percent_Identity=28.158844765343, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1789201, Length=370, Percent_Identity=25.1351351351351, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560308, Length=322, Percent_Identity=25.776397515528, Blast_Score=77, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17559526, Length=366, Percent_Identity=25.9562841530055, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=399, Percent_Identity=24.3107769423559, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24581924, Length=297, Percent_Identity=28.6195286195286, Blast_Score=89, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 96589; Mature: 96589

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: PS50075 ACP_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVVAVDVDSSMSPMSVASLAARGERPALLLRGGSIITYRELAQRVGRLASQWRGGRGLV
CEEEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCEEEHHHHHHHHHHHHHHCCCCCCEE
MLEAELSDHSIVGYLAALEAGHAVALCAGASMVTAREVFNPEYCYRRCDGRWRMERLDTD
EEEECCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCHHHHHHHCCCCEEHHHCCCC
PVDPHPQLAVLLSTSGSTGYGKCVRLSYANIQSNALSIAGYLGLSASDRGCLTLPLHYSY
CCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCEEEEEEEHHH
GLSILNTHLSVGASVYLPGSSISDDGFLDDLAESGSSSFTGVPYSYDLLEKVGFRERDFP
CHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCC
KLRFMTVAGGRMSPELVRRYDEHLSARGASFFVMYGQTEATARMAYMPPESLRGREDRIG
CEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHEEEEECCCHHHCCCCCCEE
IAIPGGSLTIVDEEGRTIQAADQAGELVYRGPNVMMGYASSRADLARGAELSELRTGDLA
EEECCCEEEEEECCCCEEEECCCCCCEEEECCCEEEECCCCHHHHHCCCCHHHHCCCCCC
SRDAEGYFRIVGRTKRLSKIAGLRIGHDSLEAALERHGISAAVVGNDTEVHAYYAGDRDP
CCCCCCEEEEECCHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCH
EEVCRLLLQASGLTAMQVGASRLNCLPRLVSGKIDYQSLAVPPAATQCRRFERQSVETVF
HHHHHHHHHHCCCHHHHHCHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHH
ARIFYPKKIRSEDSFLSLGGDSLRFVQLSIGLEKILGELPESWEKLPIGELAARSSRKGK
HHHHCHHHHCCCCCEEECCCCCEEEEEECCCHHHHHHHCCHHHHHCCCHHHHHCCCCCCC
IGQIATDLLIRALAIILVVLHHETLWPIPGGSAAMVILAGFSLARFQSGALFSGAIDKLL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHCCCCCHHHHHHHHH
WPLAQVLIPYYLIVAAYALAWGEVPWASIFLVGNFAFADPERHSMVPYLYWFIEAYSQML
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECEEECCCCCCCCHHHHHHHHHHHHHHH
LVFGALFAVPSIRRAAAKRPFAAGMILFVAAFAARMAFPLLVEIGNRQVFTLPWIFYMAV
HHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEHHHHHHHHH
LGWCAAIAATWKEKSAVMLAGTGVFLFLGLYEGVWIGTKIKYLLQIIVLAALLFLPRVRL
HHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHCCCCCC
PQWSARLILPLSAASFHIYILHRFVPELLLQPMQSLTSPVVFAVASILGGIALGAAAWLV
CCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRQLVAWLAARESRILLAPRDGPGSGLASGSPLFAVGPQEEYQIQRQ
HHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEECCCHHHHCCCC
>Mature Secondary Structure
MEVVAVDVDSSMSPMSVASLAARGERPALLLRGGSIITYRELAQRVGRLASQWRGGRGLV
CEEEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCEEEHHHHHHHHHHHHHHCCCCCCEE
MLEAELSDHSIVGYLAALEAGHAVALCAGASMVTAREVFNPEYCYRRCDGRWRMERLDTD
EEEECCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCHHHHHHHCCCCEEHHHCCCC
PVDPHPQLAVLLSTSGSTGYGKCVRLSYANIQSNALSIAGYLGLSASDRGCLTLPLHYSY
CCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCEEEEEEEHHH
GLSILNTHLSVGASVYLPGSSISDDGFLDDLAESGSSSFTGVPYSYDLLEKVGFRERDFP
CHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCC
KLRFMTVAGGRMSPELVRRYDEHLSARGASFFVMYGQTEATARMAYMPPESLRGREDRIG
CEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHEEEEECCCHHHCCCCCCEE
IAIPGGSLTIVDEEGRTIQAADQAGELVYRGPNVMMGYASSRADLARGAELSELRTGDLA
EEECCCEEEEEECCCCEEEECCCCCCEEEECCCEEEECCCCHHHHHCCCCHHHHCCCCCC
SRDAEGYFRIVGRTKRLSKIAGLRIGHDSLEAALERHGISAAVVGNDTEVHAYYAGDRDP
CCCCCCEEEEECCHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCH
EEVCRLLLQASGLTAMQVGASRLNCLPRLVSGKIDYQSLAVPPAATQCRRFERQSVETVF
HHHHHHHHHHCCCHHHHHCHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHH
ARIFYPKKIRSEDSFLSLGGDSLRFVQLSIGLEKILGELPESWEKLPIGELAARSSRKGK
HHHHCHHHHCCCCCEEECCCCCEEEEEECCCHHHHHHHCCHHHHHCCCHHHHHCCCCCCC
IGQIATDLLIRALAIILVVLHHETLWPIPGGSAAMVILAGFSLARFQSGALFSGAIDKLL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHCCCCCHHHHHHHHH
WPLAQVLIPYYLIVAAYALAWGEVPWASIFLVGNFAFADPERHSMVPYLYWFIEAYSQML
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECEEECCCCCCCCHHHHHHHHHHHHHHH
LVFGALFAVPSIRRAAAKRPFAAGMILFVAAFAARMAFPLLVEIGNRQVFTLPWIFYMAV
HHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEHHHHHHHHH
LGWCAAIAATWKEKSAVMLAGTGVFLFLGLYEGVWIGTKIKYLLQIIVLAALLFLPRVRL
HHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHCCCCCC
PQWSARLILPLSAASFHIYILHRFVPELLLQPMQSLTSPVVFAVASILGGIALGAAAWLV
CCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRQLVAWLAARESRILLAPRDGPGSGLASGSPLFAVGPQEEYQIQRQ
HHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEECCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]