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The map label for this gene is potH [H]

Identifier: 150395980

GI number: 150395980

Start: 812527

End: 813360

Strand: Direct

Name: potH [H]

Synonym: Smed_0756

Alternate gene names: 150395980

Gene position: 812527-813360 (Clockwise)

Preceding gene: 150395979

Following gene: 150395981

Centisome position: 21.48

GC content: 62.23

Gene sequence:

>834_bases
ATGCTCGCGCGCTCCCACAATCTTGGCGCGGCGCTCCTTCTGGCGCCGCTTCTCCTGCTCCTTGCCGTGGCATACGGCAT
CCCGTTTCTTGGCGTGATGAGATGGAGCTTTACTCTGCCCGAGCCAGGGCTCGCGCAATATCTTACGGTTTTCACCGACC
CGTTGGTGCAATCGGTTTTCGTGCGGACTGCGCGCATCTGTGCGCTCGTCACGGTGACGGCCGTCGCTGCTGCCTATGCC
ATTGCCTATGTTTGGGTGCGCGGCACGCCGGGTCAGCGGCTCATTGCGGAATACTGCATCCTCGTGCCGTTCTGGATCTC
GGTGCTGACCCGCGCCTTTGGCTGGGTGGCACTTCTTTCGAACCGTGGGCTCATCAATAGCTGGCTGCAGTCGCTCGGCA
TCCTCGGCGAGCCTCTCGCTCTCGTCCGCAACGAGTTCGGCGTCGTCGTCGGGATGACGCATTTCCTTATACCGTTCGCG
GTGTTTCCGATCGCATCGGCGATGCGCGCCGTCGACGAACGCGTGCTGCTTGCCGCGCGCGGTCTGGGCGCAAGCCGCAC
CCGCACCTTCTGGTCGGTATTCGTGCCTTTGACCATGTCGGGAATTATCGGCGCCGCATTGATCGTTTTCGTCTTCGCGC
TCGGCTTCTTCGTCACACCGGCAATTCTCGGCGGCGGCCGCAGCGTCATGGTGGCAGAGCTGGTCTACCTTCGCATATTC
CAGAGTCCGGACTGGGGTCTTGGTGCTGCCATCAGCGTGACCCTCGTCCTGATCGTCGGCCTGCTGACGGCAGCCCTGTT
CCGGTTTTTGCGGTTTTCCCAGATGGCAGGTTGA

Upstream 100 bases:

>100_bases
AATATGAGCAAGCAGATACCGCTTAACATGGAATGGTACGCAGAGCATTACGGGCCGGCGCTCAACGAATTCACCAAAAT
CGTTTCCGCCTGAGCCGCCG

Downstream 100 bases:

>100_bases
AGATGAGTGCATATCGTCCTGGCCCTCTTGCCCTGTTCTTCGCCTTCGTGGTGATAATCTTCCTGCTGATGCCGCTGCTG
GCTGTCGTACCGGTGTCGTT

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MLARSHNLGAALLLAPLLLLLAVAYGIPFLGVMRWSFTLPEPGLAQYLTVFTDPLVQSVFVRTARICALVTVTAVAAAYA
IAYVWVRGTPGQRLIAEYCILVPFWISVLTRAFGWVALLSNRGLINSWLQSLGILGEPLALVRNEFGVVVGMTHFLIPFA
VFPIASAMRAVDERVLLAARGLGASRTRTFWSVFVPLTMSGIIGAALIVFVFALGFFVTPAILGGGRSVMVAELVYLRIF
QSPDWGLGAAISVTLVLIVGLLTAALFRFLRFSQMAG

Sequences:

>Translated_277_residues
MLARSHNLGAALLLAPLLLLLAVAYGIPFLGVMRWSFTLPEPGLAQYLTVFTDPLVQSVFVRTARICALVTVTAVAAAYA
IAYVWVRGTPGQRLIAEYCILVPFWISVLTRAFGWVALLSNRGLINSWLQSLGILGEPLALVRNEFGVVVGMTHFLIPFA
VFPIASAMRAVDERVLLAARGLGASRTRTFWSVFVPLTMSGIIGAALIVFVFALGFFVTPAILGGGRSVMVAELVYLRIF
QSPDWGLGAAISVTLVLIVGLLTAALFRFLRFSQMAG
>Mature_277_residues
MLARSHNLGAALLLAPLLLLLAVAYGIPFLGVMRWSFTLPEPGLAQYLTVFTDPLVQSVFVRTARICALVTVTAVAAAYA
IAYVWVRGTPGQRLIAEYCILVPFWISVLTRAFGWVALLSNRGLINSWLQSLGILGEPLALVRNEFGVVVGMTHFLIPFA
VFPIASAMRAVDERVLLAARGLGASRTRTFWSVFVPLTMSGIIGAALIVFVFALGFFVTPAILGGGRSVMVAELVYLRIF
QSPDWGLGAAISVTLVLIVGLLTAALFRFLRFSQMAG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=254, Percent_Identity=30.7086614173228, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1787369, Length=220, Percent_Identity=30.9090909090909, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1787713, Length=250, Percent_Identity=25.2, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29888; Mature: 29888

Theoretical pI: Translated: 11.05; Mature: 11.05

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLARSHNLGAALLLAPLLLLLAVAYGIPFLGVMRWSFTLPEPGLAQYLTVFTDPLVQSVF
CCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VRTARICALVTVTAVAAAYAIAYVWVRGTPGQRLIAEYCILVPFWISVLTRAFGWVALLS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRGLINSWLQSLGILGEPLALVRNEFGVVVGMTHFLIPFAVFPIASAMRAVDERVLLAAR
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLGASRTRTFWSVFVPLTMSGIIGAALIVFVFALGFFVTPAILGGGRSVMVAELVYLRIF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
QSPDWGLGAAISVTLVLIVGLLTAALFRFLRFSQMAG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLARSHNLGAALLLAPLLLLLAVAYGIPFLGVMRWSFTLPEPGLAQYLTVFTDPLVQSVF
CCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VRTARICALVTVTAVAAAYAIAYVWVRGTPGQRLIAEYCILVPFWISVLTRAFGWVALLS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRGLINSWLQSLGILGEPLALVRNEFGVVVGMTHFLIPFAVFPIASAMRAVDERVLLAAR
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLGASRTRTFWSVFVPLTMSGIIGAALIVFVFALGFFVTPAILGGGRSVMVAELVYLRIF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
QSPDWGLGAAISVTLVLIVGLLTAALFRFLRFSQMAG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]