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The map label for this gene is mrpA [H]

Identifier: 150395831

GI number: 150395831

Start: 655816

End: 658191

Strand: Direct

Name: mrpA [H]

Synonym: Smed_0607

Alternate gene names: 150395831

Gene position: 655816-658191 (Clockwise)

Preceding gene: 150395829

Following gene: 150395832

Centisome position: 17.34

GC content: 62.42

Gene sequence:

>2376_bases
ATGGATGCCGCGGCCCTGACATTTCTGGCCCTCGGTCTTCCCTTTGCCGCGGCCCTCATCGCACCGGCCCTCACTCGGTT
CCTCGGCGGGAATGCTGCCTGGCTCCTGGCGCTTGCGCCATTCGCGATCTTCGTTCATTTCGCCGGCTTCGTTCCGGAAG
TCGCCGCTGGCGAAATCGTCACCGGCGGCTATGCCTGGATACCGTCGTTCAACGTCAGCTTCTCCTGGCTGATCGATGGC
TTGTCGCTTACCTTCGCTCTTCTGATTTCGGGCATCGGGACCCTGATCGTCCTTTATTCGGGCGGCTATCTGAAGGGACA
TCCCGATCAGGGCCGCTTCTTCTCGTTCATCCTGATGTTCATGGGATCGATGCAGGGGCTGGTCGTGTCGGACAGCTTCC
TGATGCTCTTCGTCTTTTGGGAACTGACGTCCATCACTTCGTTCCTGCTGATCGGCTTCGATCACCGGCGCGAGGCGGCG
CGCCGCGCGGCTTTGCAGGCCCTGGTGGTCACGGGCGGAGGCGGGCTCGCCTTGCTGGCCGGGCTTATCATCATCTGGAA
CGTCAGCGGCGTCACCCAATTCTCGCTGCTCCTGACGTTCGGCGACGAACTGAAGGAGAGCCCGTTCTATCTCGCAGCAC
TGCTCCTTGTCCTCGGCGGCGCCTTCACCAAGTCGGCACAGTTTCCGTTTCACTTCTGGCTGCCGAATGCCATGGAGGCG
CCGACTCCGGTCTCGGCTTACCTGCATTCGGCGACGATGGTGAAGGCGGGCGTCTATCTTCTCATGCGGCTCAACCCGGT
GCTCGGCGGCACGACCGAGTGGCAGGTCCTGCTGCCGCTTTTCGGTGTGACGACCCTTATTGCCGGAGCTGCGCTGGCCG
TGCGCCAAACGGATCTCAAAATGATGCTGGCCTATACGACGATGTCGTCGCTCGGCCTGCTGGTCATGTTGACCGGGCTC
GGCTTGCCCTATGCGATCGAAGCCGCCGTGCTCTATCTGGTGGCGCATGCCCTTTTCAAGGGGGCCCTGTTCATGGTGGC
CGGCATCATCGACCATGAAACCGGTACGCGCGACCTGACGAAGCTCGGCGGCCTGCGCACGGCGATGCCGTTGACCTTTG
TCATAGCTCTTACGGCGGCCTTGTCGATGGGGGGGCTGCCGCCCTTTTTCGGGTTCCTTGCAAAAGAGGAAATCTACACC
GCACTCTCCGGTTTCGACCGCCGTTCCATGGTTTTCGCGGCATTGACCATTCTCGGCAATGGGCTGATGTTCGCCGTCGG
ATTTGCGATCGCCCTGAAGCCGTTTCTCGGCCCGAAGGTCGACACGCCCAAGCCTCCCCACGAGGCTCCGGTGCTCCTCT
GGCTAGGTCCGGCCATTCTGGCCGCAAAAGGTCTTTCAATGGCCCTGATGTCGGGCTTCGTTCATCGCTACGTGACCTCG
CCCATGGCGTCGGCCGTCGCCGGCGAGCCGAGAAGCGCCACGATATCGCTGGTGCCGCATTTCGGCGTGGCGCTGTTGCT
TTCCGTCGCGACGCTGGCGGTCGGCGTCGTTCTGTTCCTCAATCTGGCTCGGCTGCGCGCGGCCATGGTGGCGGTTCTTG
CGGATATCGGCTGGGGACCCGATCGGGGCTTCGACCAGTTCATGCGTGGCCTCGTGCGCTTTGCGGTCACCGCCACCCGT
CGACTGCAGGGGGGCCGGCTCGACACCTATATGACCATGACCTTCGTGCTGGTCGCCGCCGTCATGCTGGCGTTGCCGCT
GCGCTATGGCGAATTCCCGCGCGCGCCGTTCTTTCCGGCGGACGTTCCGCTCCACGAATTCGCGATCATGGCGATCGCAG
CCCTTGGCCTCTTCGCCGTGGTGATGGCCGCGGATCGGCTGACGGCGATCGTCTCCCTCGGCATTCAGGGCTTTGCCGTG
GCGATGCTCTTTCTGCTTTACGGCGCCCCCGATCTTGCCTTCACCCAATTCATGATAGAGACACTGTCGGTCGTCGTTCT
GGCGCTGGTGATGACGCGGTTGCGGCTGTCTCCCTCGGACCACAGGCCGCTCAGACAGAAGCTGCCCGACTTTACAATCG
CGCTCGCCTGCGGAGCCGGGTTCGGTCTTTTCCTCCTGAGGGTCACCGGCGTGCCGTTCGATTCGACGCTGACGGACTTT
TTCAATCTCTATTCGAAGTCGATCGCGCACGGTGCAAATGTAGTCAACGTCATCATAGTCGACTTCCGCGGCACGGATAC
GCTCGGCGAAATTGCGGTGGTGCTGGTTGCCGGCCTGTCAATCCTTTCGCTCGTGCGGCTGCGGGCGGGATCGTTGAAAC
GTGGTGAGCTGACCGAGATTGCGGCCGCCGATGCGAGTTCGAAGGAGCTGGTATGA

Upstream 100 bases:

>100_bases
CTTCGCAACGAGGCCGATTTCCCTTGAGGCGGGCCCGCCCTTCTTCTATGTCCGGTGGAAGATTGACTGAACGATCAATG
AGAAGGAGCGTGTCGACACG

Downstream 100 bases:

>100_bases
AGTCGATTATATTTCGGACCGTCGCCCCCTTCCTCACCGGATTGATGATGCTCTTTTCGATCTTCGTTCTGCTGCGCGGC
CACAATGAACCCGGGGGCGG

Product: putative monovalent cation/H+ antiporter subunit A

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 791; Mature: 791

Protein sequence:

>791_residues
MDAAALTFLALGLPFAAALIAPALTRFLGGNAAWLLALAPFAIFVHFAGFVPEVAAGEIVTGGYAWIPSFNVSFSWLIDG
LSLTFALLISGIGTLIVLYSGGYLKGHPDQGRFFSFILMFMGSMQGLVVSDSFLMLFVFWELTSITSFLLIGFDHRREAA
RRAALQALVVTGGGGLALLAGLIIIWNVSGVTQFSLLLTFGDELKESPFYLAALLLVLGGAFTKSAQFPFHFWLPNAMEA
PTPVSAYLHSATMVKAGVYLLMRLNPVLGGTTEWQVLLPLFGVTTLIAGAALAVRQTDLKMMLAYTTMSSLGLLVMLTGL
GLPYAIEAAVLYLVAHALFKGALFMVAGIIDHETGTRDLTKLGGLRTAMPLTFVIALTAALSMGGLPPFFGFLAKEEIYT
ALSGFDRRSMVFAALTILGNGLMFAVGFAIALKPFLGPKVDTPKPPHEAPVLLWLGPAILAAKGLSMALMSGFVHRYVTS
PMASAVAGEPRSATISLVPHFGVALLLSVATLAVGVVLFLNLARLRAAMVAVLADIGWGPDRGFDQFMRGLVRFAVTATR
RLQGGRLDTYMTMTFVLVAAVMLALPLRYGEFPRAPFFPADVPLHEFAIMAIAALGLFAVVMAADRLTAIVSLGIQGFAV
AMLFLLYGAPDLAFTQFMIETLSVVVLALVMTRLRLSPSDHRPLRQKLPDFTIALACGAGFGLFLLRVTGVPFDSTLTDF
FNLYSKSIAHGANVVNVIIVDFRGTDTLGEIAVVLVAGLSILSLVRLRAGSLKRGELTEIAAADASSKELV

Sequences:

>Translated_791_residues
MDAAALTFLALGLPFAAALIAPALTRFLGGNAAWLLALAPFAIFVHFAGFVPEVAAGEIVTGGYAWIPSFNVSFSWLIDG
LSLTFALLISGIGTLIVLYSGGYLKGHPDQGRFFSFILMFMGSMQGLVVSDSFLMLFVFWELTSITSFLLIGFDHRREAA
RRAALQALVVTGGGGLALLAGLIIIWNVSGVTQFSLLLTFGDELKESPFYLAALLLVLGGAFTKSAQFPFHFWLPNAMEA
PTPVSAYLHSATMVKAGVYLLMRLNPVLGGTTEWQVLLPLFGVTTLIAGAALAVRQTDLKMMLAYTTMSSLGLLVMLTGL
GLPYAIEAAVLYLVAHALFKGALFMVAGIIDHETGTRDLTKLGGLRTAMPLTFVIALTAALSMGGLPPFFGFLAKEEIYT
ALSGFDRRSMVFAALTILGNGLMFAVGFAIALKPFLGPKVDTPKPPHEAPVLLWLGPAILAAKGLSMALMSGFVHRYVTS
PMASAVAGEPRSATISLVPHFGVALLLSVATLAVGVVLFLNLARLRAAMVAVLADIGWGPDRGFDQFMRGLVRFAVTATR
RLQGGRLDTYMTMTFVLVAAVMLALPLRYGEFPRAPFFPADVPLHEFAIMAIAALGLFAVVMAADRLTAIVSLGIQGFAV
AMLFLLYGAPDLAFTQFMIETLSVVVLALVMTRLRLSPSDHRPLRQKLPDFTIALACGAGFGLFLLRVTGVPFDSTLTDF
FNLYSKSIAHGANVVNVIIVDFRGTDTLGEIAVVLVAGLSILSLVRLRAGSLKRGELTEIAAADASSKELV
>Mature_791_residues
MDAAALTFLALGLPFAAALIAPALTRFLGGNAAWLLALAPFAIFVHFAGFVPEVAAGEIVTGGYAWIPSFNVSFSWLIDG
LSLTFALLISGIGTLIVLYSGGYLKGHPDQGRFFSFILMFMGSMQGLVVSDSFLMLFVFWELTSITSFLLIGFDHRREAA
RRAALQALVVTGGGGLALLAGLIIIWNVSGVTQFSLLLTFGDELKESPFYLAALLLVLGGAFTKSAQFPFHFWLPNAMEA
PTPVSAYLHSATMVKAGVYLLMRLNPVLGGTTEWQVLLPLFGVTTLIAGAALAVRQTDLKMMLAYTTMSSLGLLVMLTGL
GLPYAIEAAVLYLVAHALFKGALFMVAGIIDHETGTRDLTKLGGLRTAMPLTFVIALTAALSMGGLPPFFGFLAKEEIYT
ALSGFDRRSMVFAALTILGNGLMFAVGFAIALKPFLGPKVDTPKPPHEAPVLLWLGPAILAAKGLSMALMSGFVHRYVTS
PMASAVAGEPRSATISLVPHFGVALLLSVATLAVGVVLFLNLARLRAAMVAVLADIGWGPDRGFDQFMRGLVRFAVTATR
RLQGGRLDTYMTMTFVLVAAVMLALPLRYGEFPRAPFFPADVPLHEFAIMAIAALGLFAVVMAADRLTAIVSLGIQGFAV
AMLFLLYGAPDLAFTQFMIETLSVVVLALVMTRLRLSPSDHRPLRQKLPDFTIALACGAGFGLFLLRVTGVPFDSTLTDF
FNLYSKSIAHGANVVNVIIVDFRGTDTLGEIAVVLVAGLSILSLVRLRAGSLKRGELTEIAAADASSKELV

Specific function: Mnh complex is a Na(+)Li(+)/H(+) antiporter involved in Na(+) and/or Li(+) excretion and Na(+) resistance. Na(+)/H(+) antiport consumes a transmembrane electrical potential, and is thus inferred to be electrogenic. Does not transport K(+), Ca(2+) or Mg(2+

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=347, Percent_Identity=35.1585014409222, Blast_Score=179, Evalue=1e-44,
Organism=Homo sapiens, GI251831116, Length=377, Percent_Identity=26.2599469496021, Blast_Score=83, Evalue=1e-15,
Organism=Escherichia coli, GI1788614, Length=350, Percent_Identity=37.1428571428571, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1788829, Length=348, Percent_Identity=35.9195402298851, Blast_Score=180, Evalue=3e-46,
Organism=Escherichia coli, GI1788831, Length=352, Percent_Identity=31.25, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1788613, Length=337, Percent_Identity=29.673590504451, Blast_Score=117, Evalue=4e-27,
Organism=Escherichia coli, GI1788827, Length=439, Percent_Identity=25.9681093394077, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI145693160, Length=330, Percent_Identity=26.6666666666667, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI2367154, Length=215, Percent_Identity=28.8372093023256, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 84253; Mature: 84253

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAAALTFLALGLPFAAALIAPALTRFLGGNAAWLLALAPFAIFVHFAGFVPEVAAGEIV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
TGGYAWIPSFNVSFSWLIDGLSLTFALLISGIGTLIVLYSGGYLKGHPDQGRFFSFILMF
ECCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCEECCCCCCHHHHHHHHHH
MGSMQGLVVSDSFLMLFVFWELTSITSFLLIGFDHRREAARRAALQALVVTGGGGLALLA
HCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEECCCHHHHHH
GLIIIWNVSGVTQFSLLLTFGDELKESPFYLAALLLVLGGAFTKSAQFPFHFWLPNAMEA
HHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCC
PTPVSAYLHSATMVKAGVYLLMRLNPVLGGTTEWQVLLPLFGVTTLIAGAALAVRQTDLK
CCCHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MMLAYTTMSSLGLLVMLTGLGLPYAIEAAVLYLVAHALFKGALFMVAGIIDHETGTRDLT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
KLGGLRTAMPLTFVIALTAALSMGGLPPFFGFLAKEEIYTALSGFDRRSMVFAALTILGN
HHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
GLMFAVGFAIALKPFLGPKVDTPKPPHEAPVLLWLGPAILAAKGLSMALMSGFVHRYVTS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECHHHHHHCCHHHHHHHHHHHHHHHH
PMASAVAGEPRSATISLVPHFGVALLLSVATLAVGVVLFLNLARLRAAMVAVLADIGWGP
HHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DRGFDQFMRGLVRFAVTATRRLQGGRLDTYMTMTFVLVAAVMLALPLRYGEFPRAPFFPA
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DVPLHEFAIMAIAALGLFAVVMAADRLTAIVSLGIQGFAVAMLFLLYGAPDLAFTQFMIE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHH
TLSVVVLALVMTRLRLSPSDHRPLRQKLPDFTIALACGAGFGLFLLRVTGVPFDSTLTDF
HHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCHHHHHH
FNLYSKSIAHGANVVNVIIVDFRGTDTLGEIAVVLVAGLSILSLVRLRAGSLKRGELTEI
HHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
AAADASSKELV
HHCCCCCCCCC
>Mature Secondary Structure
MDAAALTFLALGLPFAAALIAPALTRFLGGNAAWLLALAPFAIFVHFAGFVPEVAAGEIV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
TGGYAWIPSFNVSFSWLIDGLSLTFALLISGIGTLIVLYSGGYLKGHPDQGRFFSFILMF
ECCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCEECCCCCCHHHHHHHHHH
MGSMQGLVVSDSFLMLFVFWELTSITSFLLIGFDHRREAARRAALQALVVTGGGGLALLA
HCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEECCCHHHHHH
GLIIIWNVSGVTQFSLLLTFGDELKESPFYLAALLLVLGGAFTKSAQFPFHFWLPNAMEA
HHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCC
PTPVSAYLHSATMVKAGVYLLMRLNPVLGGTTEWQVLLPLFGVTTLIAGAALAVRQTDLK
CCCHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MMLAYTTMSSLGLLVMLTGLGLPYAIEAAVLYLVAHALFKGALFMVAGIIDHETGTRDLT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
KLGGLRTAMPLTFVIALTAALSMGGLPPFFGFLAKEEIYTALSGFDRRSMVFAALTILGN
HHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
GLMFAVGFAIALKPFLGPKVDTPKPPHEAPVLLWLGPAILAAKGLSMALMSGFVHRYVTS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECHHHHHHCCHHHHHHHHHHHHHHHH
PMASAVAGEPRSATISLVPHFGVALLLSVATLAVGVVLFLNLARLRAAMVAVLADIGWGP
HHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DRGFDQFMRGLVRFAVTATRRLQGGRLDTYMTMTFVLVAAVMLALPLRYGEFPRAPFFPA
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DVPLHEFAIMAIAALGLFAVVMAADRLTAIVSLGIQGFAVAMLFLLYGAPDLAFTQFMIE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHH
TLSVVVLALVMTRLRLSPSDHRPLRQKLPDFTIALACGAGFGLFLLRVTGVPFDSTLTDF
HHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCHHHHHH
FNLYSKSIAHGANVVNVIIVDFRGTDTLGEIAVVLVAGLSILSLVRLRAGSLKRGELTEI
HHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
AAADASSKELV
HHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11356194 [H]