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The map label for this gene is miaB [H]

Identifier: 150395268

GI number: 150395268

Start: 41068

End: 42486

Strand: Direct

Name: miaB [H]

Synonym: Smed_0040

Alternate gene names: 150395268

Gene position: 41068-42486 (Clockwise)

Preceding gene: 150395267

Following gene: 150395269

Centisome position: 1.09

GC content: 62.86

Gene sequence:

>1419_bases
ATGACCCAGGAAACCGCTCTTTTGCCGACGTCGCCCGAAGCGGGCGACCTGAACGTCCCGGCGCGCAAGGTCTTCGTCAA
GACTTACGGCTGCCAGATGAACGTCTATGACTCCGATCGCATGTCGGATGCGCTGTCGCGCGACGGCTATGTTGCGACCG
ATGTCTTGGAAGAGGCCGATTTTGTCCTGCTCAATACCTGCCATATTCGCGAAAAGGCCGCAGAGAAGGTCTATTCGGAG
CTCGGCCGCCTGCGTGACCTGAAGAAGGCGAAGGCGCGCGAGGGCCGCGAGATGCTGATCGGCGTTGCCGGTTGCGTCGC
CCAGGCCGAGGGCAAGGAGATTCTCCGCCGGGCGCCTGCAGTCGATCTTGTCATTGGGCCGCAGACCTACCACCGCCTCC
CGGAAGCGCTGAGGCGGGCCCGCACCGGCGAGCGCATCGTGGAAACGGATTATGCCATCGAGGACAAGTTCGTCCATTTG
CCGGCGCCAGACAAGGCCAAGACTCGTGCGCGCGGCGTTACGGCCTTCCTGACGGTACAGGAAGGCTGCGACAAGTTCTG
TACCTTCTGCGTCGTTCCCTACACACGCGGCTCGGAAGTCTCACGTCCCGTGGCACAGATCGTCGCGGAGGCGGAGAAGC
TCGTTGAGGGCGGCGTGCGCGAAATCACCCTGCTCGGTCAGAATGTCAACGCCTGGCACGGTGCCGGTCCGCACGGCCGC
GAATGGGGGCTCGGCGATCTCTTGCGGCGTCTCGGCGAGATCGAGGGGCTCGCGCGCCTGCGATACACCACCAGCCACCC
CCGCGACATGGACGACAGCCTGATCGAGGCGCATCGGTCGATGGCGAAGCTGATGCCCTATCTGCATCTGCCGGTGCAGT
CAGGCTCGGATCGTATCCTGAAGGCGATGAACCGGCGCCATACGGCAGCCGAATATCTGGCGCTCGTCGCGCGCATTCGA
GCGGTGCAGCCGGACCTTGCGCTTTCGGGCGACTTCATCGTCGGCTTCCCCGGCGAGACCGATGAGGACTTCGAGGATAC
GCTGCGCCTTGTAGATGCCGTGAACTATGCACAGGCTTTTTCGTTTAAGTACTCGACGCGTCCCGGCACGCCGGGCGCCG
AACTCGAGCAACAGGTTCCCGAGGACGTGAAGGCGAAGAGATTGGAAATATTGCAAGCTTTGCTCATCAAGCAGCAGCGC
GGCTTCGCCGAAGCCTGCGTCGGCCGCGAGATCGACCTGCTGCTGGAGAAGCCGGGCCGCATGCCCGGGCAGCTCGTCGG
TCGCTCGCCATGGCTGCAGCCCGTGAATGTTGATGCAAAAGCATCGCAAATCGGTGACATTATCAGAGTGCGAATCACCA
AGGCCGGGCCGAACAGCCTGTTTGCCGAGATGATCGGGGAAACGGAAGCAAGGAGCTGA

Upstream 100 bases:

>100_bases
CTCATTCAGGGATCACCCTGAAACGACGGTTGATATCGACCGGCGCCCTACCGGCCCGAAAATGCTTCAAATTTGGGGCG
CTATGCACTAAAGAGCCGGC

Downstream 100 bases:

>100_bases
CCGCTTGAACGGACACGAACTGATTTCTTCCTCACCGCGCCAGTCGAAGACATCCGCGACGGACGCCAATCACTTCGTGC
TCACCTTCGAGAACAACCGC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTQETALLPTSPEAGDLNVPARKVFVKTYGCQMNVYDSDRMSDALSRDGYVATDVLEEADFVLLNTCHIREKAAEKVYSE
LGRLRDLKKAKAREGREMLIGVAGCVAQAEGKEILRRAPAVDLVIGPQTYHRLPEALRRARTGERIVETDYAIEDKFVHL
PAPDKAKTRARGVTAFLTVQEGCDKFCTFCVVPYTRGSEVSRPVAQIVAEAEKLVEGGVREITLLGQNVNAWHGAGPHGR
EWGLGDLLRRLGEIEGLARLRYTTSHPRDMDDSLIEAHRSMAKLMPYLHLPVQSGSDRILKAMNRRHTAAEYLALVARIR
AVQPDLALSGDFIVGFPGETDEDFEDTLRLVDAVNYAQAFSFKYSTRPGTPGAELEQQVPEDVKAKRLEILQALLIKQQR
GFAEACVGREIDLLLEKPGRMPGQLVGRSPWLQPVNVDAKASQIGDIIRVRITKAGPNSLFAEMIGETEARS

Sequences:

>Translated_472_residues
MTQETALLPTSPEAGDLNVPARKVFVKTYGCQMNVYDSDRMSDALSRDGYVATDVLEEADFVLLNTCHIREKAAEKVYSE
LGRLRDLKKAKAREGREMLIGVAGCVAQAEGKEILRRAPAVDLVIGPQTYHRLPEALRRARTGERIVETDYAIEDKFVHL
PAPDKAKTRARGVTAFLTVQEGCDKFCTFCVVPYTRGSEVSRPVAQIVAEAEKLVEGGVREITLLGQNVNAWHGAGPHGR
EWGLGDLLRRLGEIEGLARLRYTTSHPRDMDDSLIEAHRSMAKLMPYLHLPVQSGSDRILKAMNRRHTAAEYLALVARIR
AVQPDLALSGDFIVGFPGETDEDFEDTLRLVDAVNYAQAFSFKYSTRPGTPGAELEQQVPEDVKAKRLEILQALLIKQQR
GFAEACVGREIDLLLEKPGRMPGQLVGRSPWLQPVNVDAKASQIGDIIRVRITKAGPNSLFAEMIGETEARS
>Mature_471_residues
TQETALLPTSPEAGDLNVPARKVFVKTYGCQMNVYDSDRMSDALSRDGYVATDVLEEADFVLLNTCHIREKAAEKVYSEL
GRLRDLKKAKAREGREMLIGVAGCVAQAEGKEILRRAPAVDLVIGPQTYHRLPEALRRARTGERIVETDYAIEDKFVHLP
APDKAKTRARGVTAFLTVQEGCDKFCTFCVVPYTRGSEVSRPVAQIVAEAEKLVEGGVREITLLGQNVNAWHGAGPHGRE
WGLGDLLRRLGEIEGLARLRYTTSHPRDMDDSLIEAHRSMAKLMPYLHLPVQSGSDRILKAMNRRHTAAEYLALVARIRA
VQPDLALSGDFIVGFPGETDEDFEDTLRLVDAVNYAQAFSFKYSTRPGTPGAELEQQVPEDVKAKRLEILQALLIKQQRG
FAEACVGREIDLLLEKPGRMPGQLVGRSPWLQPVNVDAKASQIGDIIRVRITKAGPNSLFAEMIGETEARS

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=485, Percent_Identity=35.0515463917526, Blast_Score=285, Evalue=8e-77,
Organism=Homo sapiens, GI28872782, Length=485, Percent_Identity=35.0515463917526, Blast_Score=285, Evalue=9e-77,
Organism=Homo sapiens, GI93277076, Length=473, Percent_Identity=26.8498942917548, Blast_Score=169, Evalue=4e-42,
Organism=Escherichia coli, GI1786882, Length=449, Percent_Identity=47.8841870824053, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI1787057, Length=463, Percent_Identity=30.4535637149028, Blast_Score=189, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17553146, Length=486, Percent_Identity=33.9506172839506, Blast_Score=258, Evalue=6e-69,
Organism=Caenorhabditis elegans, GI71996771, Length=368, Percent_Identity=26.9021739130435, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI21356207, Length=481, Percent_Identity=32.2245322245322, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI19922432, Length=459, Percent_Identity=28.1045751633987, Blast_Score=160, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 52240; Mature: 52109

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQETALLPTSPEAGDLNVPARKVFVKTYGCQMNVYDSDRMSDALSRDGYVATDVLEEAD
CCCCCCCCCCCCCCCCCCCCHHHEEEEEECCEEECCCCHHHHHHHHCCCCCHHHHHHCCC
FVLLNTCHIREKAAEKVYSELGRLRDLKKAKAREGREMLIGVAGCVAQAEGKEILRRAPA
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
VDLVIGPQTYHRLPEALRRARTGERIVETDYAIEDKFVHLPAPDKAKTRARGVTAFLTVQ
CEEEECCHHHHHHHHHHHHHHCCCCCHHCCCEECCCEEECCCCCHHHHHHCCCEEEEEHH
EGCDKFCTFCVVPYTRGSEVSRPVAQIVAEAEKLVEGGVREITLLGQNVNAWHGAGPHGR
HHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCC
EWGLGDLLRRLGEIEGLARLRYTTSHPRDMDDSLIEAHRSMAKLMPYLHLPVQSGSDRIL
CCCHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
KAMNRRHTAAEYLALVARIRAVQPDLALSGDFIVGFPGETDEDFEDTLRLVDAVNYAQAF
HHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHH
SFKYSTRPGTPGAELEQQVPEDVKAKRLEILQALLIKQQRGFAEACVGREIDLLLEKPGR
EEEECCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHCCCCC
MPGQLVGRSPWLQPVNVDAKASQIGDIIRVRITKAGPNSLFAEMIGETEARS
CCHHHCCCCCCCCCCCCCCHHHHHCCEEEEEEECCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TQETALLPTSPEAGDLNVPARKVFVKTYGCQMNVYDSDRMSDALSRDGYVATDVLEEAD
CCCCCCCCCCCCCCCCCCCHHHEEEEEECCEEECCCCHHHHHHHHCCCCCHHHHHHCCC
FVLLNTCHIREKAAEKVYSELGRLRDLKKAKAREGREMLIGVAGCVAQAEGKEILRRAPA
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
VDLVIGPQTYHRLPEALRRARTGERIVETDYAIEDKFVHLPAPDKAKTRARGVTAFLTVQ
CEEEECCHHHHHHHHHHHHHHCCCCCHHCCCEECCCEEECCCCCHHHHHHCCCEEEEEHH
EGCDKFCTFCVVPYTRGSEVSRPVAQIVAEAEKLVEGGVREITLLGQNVNAWHGAGPHGR
HHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCC
EWGLGDLLRRLGEIEGLARLRYTTSHPRDMDDSLIEAHRSMAKLMPYLHLPVQSGSDRIL
CCCHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
KAMNRRHTAAEYLALVARIRAVQPDLALSGDFIVGFPGETDEDFEDTLRLVDAVNYAQAF
HHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHH
SFKYSTRPGTPGAELEQQVPEDVKAKRLEILQALLIKQQRGFAEACVGREIDLLLEKPGR
EEEECCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHCCCCC
MPGQLVGRSPWLQPVNVDAKASQIGDIIRVRITKAGPNSLFAEMIGETEARS
CCHHHCCCCCCCCCCCCCCHHHHHCCEEEEEEECCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]